Preparation of conjugate antigen and antibody to parathion

  Objective   To produce the organophate parathion conjugate antigen antibody.

  Methods   The hapten, which reduces the parathion changing-NO₂ into-NH₂, is conjugated diazo-linked with the carrier protein BSA to prepare conjugate antigen.By immunizating the New Zealand rabbit, the antisera is obtained.

  Results   The result by NMR is δ: 6.9-7 (m, 2H, CH₂)6.4-6.5(m, 2H, CH₂)4.1-4.2(q, 4H, -CH2)3.4(s, 2H, NH₂)1.3-1.4(t, 6H, CH₃).The ratio of hapten to BSA is from 35-36 to 1.Test of the antisera by indirected ELISA and immunodiffusion shows that the titer is high and antibody is specific.The titer is 1:128 000.Precipitin bands are observed from the reaction of the antisera with the conjugate by immunodiffusion.The minimum concentration of ELISA inhibition for antisera is 0.16 ng/ml.There is no cross-reactivity between the antisera and serum analogues.The research confirms that the antisera is specific to the parathion.

  Conclusion   The antigen is in effect.The research provides the preparation for producing McAb of parathion and sets up the ELISA method of detecting parathion.