Immune response induced by herpes simplex virus-2 DNA vaccine in mice

  Objective   To evaluate the protective efficacy of DNA vaccine expressing herpes simplex type 2(HSV-2) full-length glycoprotein D(gD).

  Methods   The whole encoding sequence of gD was amplified from HSV-2 genome by PCR, and then cloned into downstream of PCMV of pcDNA3, and resulted in the HSV-2 DNA vaccince-pcDNA3-gD.pcDNA3-gD was inoculated to mice by bilateral intramuscular injection into the rear legs.

  Results   The mice inoculated with pcDNA3-gD could produce specific antibodies and survive after the lethal challenge with HSV-2.The rate of survival rate was 75%.

  Conclusion   pcDNA3-gD can induce specific immune response and may be a candidate DNA vaccine D.