Objective To search aninstant early and rapid diagnostic method to determine coronavirus.
Methods Firstly, mouthwash(NPA)fluid is collected from 163 SARS patients, 28 suspected cases, 43 medically observatory cases and 33 health individuals.FQ-PCR and reagent kits are used to test coronavirus.
Results There are 93(57.1%)SARS patients hospitalized within three days and 70(42.9%)SARS patients hospitalized within four to twenty days.53 of the 93 patients are tested with positive coronavirus.The total positive rate is 57.0%.Furthermore, the positive rate in the first, second and third day of hospitalization are 50.0%, 69.2% and 45.5% respectively.The total positive rate of the 70 patients hospitalized more than four days is only 7.1% That is 3.4% among 28 suspected cases.The positive rate is negative among the 17 hospitalized cases with fever and waiting for determination, 43 medically observatory cases and 33 health individuals.Through testing coronavirus in mouthw ash fluid in different incidence time, it can be found that virus positive rate of the coronavirus is highest in the second day of hospitalization.The positive rate begins to decline in the third day of hospitalization and reaches the lowest level after the fourth day of hospitalization.
Conclusion The method has high sensitivity, specificity and can detect at least one copy of gene.It can meet the demands for clinical diagnosis as well as screening and detecting medically observatory cases and closely contacting persons.It can be used at all times to determine the quantities exactly with little error.The method is used under full-sealed conditions, which can eliminate false positive result.The package is for one person with stable quality and facility in use.It satisfies the demands of Ministry of Public Health and Supervisory of National Food and Drug A dministrative Bureau.It can be used in clinical diagnosis extensively.
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