Effects of sulphur dioxide inhalation on activities of GST, G6PD and LPO in mice

  Objective   To investigate the effects of sulphur dioxide inhalation on the activities of GST, G6PD and changes of lipid peroxidation in male mice.

  Methods   Sixty male Kunming strain mice were randomly divided into three large groups, each one is twenty.Each large group was composed of sulphur dioxide inhalation sub-group and control sub-group.Sulphur dioxide inhalation concentrations in three groups were respectively(22±2)mg/m³(low group), (64±3)mg/m³(medium group), (148±23)mg/m³(high group).At the end of experiments the activities of GST, G6PD and content of TBARS were measured in brain, lung, heart, liver, kidney of mice respectively.

  Results   In the case of sulfur dioxide inhalation concentration as 148±23 mg/m³, the activities of GST, G6PD in brain, lung, heart, liver, kidney of mice were observed significantly decreased(P < 0.01 or P < 0.05).The TBARS levels in brain, lung, heart, liver, kidney were significantly increased(P < 0.01 or P < 0.05).

  Conclusion   Sulphur dioxide inhalation can induce injury of mice through the oxidative stress, which was a severe effect of the activity changes of GST, G6PD and TBARS.Sulphur dioxide can induce oxidative damage of many organs in mice.