Objective To study the effects of 9-A Con apoptosis in HL-60 cells and possible intracellular factors involved in the process.
Methods HL-60 cells were treated with 9-AC to induce cell apotosis.Three international accepted criteria for monitoring apoptosis were adopted, including transmission electron microscopy, agarosegel DNA electrophoresis, and fluorescence-activated cell sorter(FACS).
Results 9-AC could induce apoptosis of HL-60 cells, with its concentration most opt imal at 5×10-4 mol/L.Forty-eight hours treatment of HL-60 cells with 9-AC at above concentration induced remar kable apoptotic phenomena as typified as: Ultrastr uctural changes formed the so-called apoptotic bodies, typical DNA ladder, symbolizing inter nucleosomal fragmentation of DNA were observed by using agarosegel electrophoresis of isolated DNA from HL-60 cells.FACS analysis revealed markedly apoptotic peak-subdiploid peak.
Conclusion 9-AC could induce apoptosis of HL-60 cells.