Effect of magnesium supplementation on heat shock gene expression in endothelial cells induced by H2O2

  Objective   The effect of magnesium on heat shock gene expression in endothelial cells induced by H₂O₂ was studied.

  Methods   The cultured human umbilical veinendothelial cells were divided into 4 groups(group of control, group of H₂O₂, group of Mg²⁺ and group of H₂O₂+Mg²⁺).The extent of cellular lipid peroxides was deter mined by measuring the for mation of TBARS, and expression of HSP70 by the method of immunohisto chemistry staining.

  Results   Compared with the group of H₂O₂, the formation of TBARS of groups H₂O₂+Mg²⁺ at all doses was decreased(P < 0.05), and the expression of HSP in the groups H₂O₂+Mg²⁺ at all doses increased significantly(P < 0.05, P < 0.01).

  Conclusion   The effect of magnesium on protecting cells from oxidative stress was probably related to its action on heat shock gene expression.