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ZHANG Hong-wei, XU Yong-jun, ZHANG Li-xia, . Construction of ScFv gene fragment of mouse[J]. Chinese Journal of Public Health, 2003, 19(5): 567-568.
Citation: ZHANG Hong-wei, XU Yong-jun, ZHANG Li-xia, . Construction of ScFv gene fragment of mouse[J]. Chinese Journal of Public Health, 2003, 19(5): 567-568.
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Construction of ScFv gene fragment of mouse

    Abstract
  •   Objective   To assemble the antibody heavy chain and light chain variable domains by the molecular recombinant technology.
      Methods   Using the PCR, immuno globulin variable(V)region genes are amplified from spleen cells.The VH(variable heav y)and VL(variable light)genes are then linked into ScFv.
      Results   The VH and VL gene are cloned and the ScFv gene fragment are assembled.The fregment is 750 bp.
      Conclusion   ScFv is small molecular and ligated to phage vector and screen fusion protein.The technology is a simple and potential method to make antibody.
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