Relationship between magnesium and multiplex polymerase chain reaction

  Objective   To get the relationship between magnesium and multiplex polymer ase chain reaction.

  Methods   With different magnesium concentration of buffer and with different magnesium concentration of buffer that without KCl to perform multiplex polymerase cthain reaction.

  Results   At the higher magnesium concentration unspecific products appeared, but they gradually decreased in intensity tow ards 10 mmol/L mag nesium concentrations.At 5 mmol/L mag nesium concentration without KCl salt, there were specific products, but increasing with the magnesium concentration, at 50 mmol/L, the multiplex polymerase chain reaction was completely inhibited, there were no specific products.

  Conclusion   The concentration of magnesium was necessary for Tag DNA polymerase.Tag DNA polymerase required free magnesium.2 mmol/L concentration of magnesium was very best for the five genes multiplex polymerase chain reaction.