Expression of nerve growth factor induced by permethrin treatments

  Objective   To elucidate the mechanism of per methrin on central nervous system(CNS).

  Methods   The levels of brain-derived neurotrophic factor(BDNF)mRNA and protein expression in ratcor tex and hippocampus were examined by RT-PCR, dot blot, flow cytometry analysis and immunochemistry.

  Results   RT-PCR and dot blot analysis indicated the levels of BDNFmRNF expression in rat brain were up-regulated by permethrin(PM)administration at a high dose(PM1, 400mg/kg°d, ip)one times and a low dose(PM2, 200mg/kg°d, ip)for 5 days, especially in cortex.However the protein expression of BDNF was not correspond to the level of BDNF mRNA: in spite of marked increase of BDNF protein expression of rat cortex in PM1 and PM2 groups were observed by flow cytometry analysis and immunochemistry, the levels of BDNF protein in rathippocampus remained unchanged except CA1 region in PM1 group.

  Conclusion   The involvement of induction of BDNF expression in ratbrain may play a role in neur oprotective profiles of CNS injury.