Effect of ethanol on apoptosis in spermatogenic cells

  Objective   To study the effect of drinkingalcohol heavily on expression of Bcl-2 and Bax in spermatogenic cells.

  Methods   The animal model of drinking alcohol heavily was administered alcohol through gastric tube in rats.Then the expression of Bcl-2 and Bax in spermatogenic cells were investigated with the immunohistochemistry and image analysis.

  Results   The positive cell expression of bcl-2 in each genocyte was 96±33.74, 156.6±28.52 respectively, which was lower than the control(222.6±56.86, P < 0.01).Over doses use of alcohol for a long time could obviously reduce the number of cells of expressing Bcl-2 and the intensity of its expression(P < 0.01), and increase the number of cells of expressing Bax and the intensity of its ex pression in gonocyte(P < 0.01), increased the number of apoptosis of spermatogenic cells.

  Conclusion   Alcohol could reduced the expression of bcl-2, promoted the expression of Bax in gonocyte and the apoptosis of gonocyte.