Study on inhibition of mammary tumor by flavonoids crude extract in wheat germ

  Objective   To investigate whether the flavonoids crude extrct in wheat germ could inhibite the mammary tumor induced by 7, 12-dimethlben z〔a〕anthracene and clarify the possible inhibitory machenism.

  Methods   Seventy female Sprague-Dawley(SD)rats aged 50 days were randomly divided into four groups: control group(10), mammary tumour group(20), low dose experimental group(20)and high dose experimental group(20).Except of rats in control group, each rat in other groups was given 15 mg 7, 12-dimethylben z〔a〕 anthracene(DMBA)dissolved in 1.5ml plantoil for twice every other two week by gavage.While the rats in low and high dose experimental groups were given forage contained of 2% and 10% flavonoids crude extract of wheat germ respectively, rats in control and mammary tumor groups were given basic forage.After 24 weeks administered DMBA to rats, the glutathione peroxidase(GSH-Px), superoxide dismutase(SOD), glutathione Stransferase(GST)activity and glutathion(GSH), malondialdehyde(MDA)level were measured respectively in blood and liver of rats.

  Results   The flavonoids crude extract could enhance the GSH-Px, SOD, GST activity and GSH level in blood and liver of rats, decrease the MDA level(P < 0.05).

  Conclusion   The flavonoids crude extracthad the anti-oxidation, which demonstrated one of mechanisms of chemophylactic effects on mammary tumor.