The Foundation of Erythrocyte Membranes Lipid Peroxidation Model Induced by Vc/FeSO4

The condition and thetime course of lipid peroxidation(LP) induced by Vc /FeSO₄ in human erytrocytem embranes invitrow as studied. The results revealed that, when human erythoccytem embranes(0.75mg/ml protein) were incubated with 5μmol/L PBS buffer for 5 minutes, Vc, at low concentration(<1.0 mmol/L) stimulate lP, but at high concenration(>1.mmol/L), inhibated LP. The content of malonaldehyde(MDA) rised rapidly after additon of 0.25mmol/L Vc and 5.0μmol/L FeSO₄, reached maxinal level in 90minutes, very little additional LP occured over the next two hours. The result suggested that in 45 minutes, Vc /FeSO₄ the appropriate scale could induced effectively erythrocytem embranes LP. A model induced by Vc /FeSO₄ was founded preliminarily in our experimnet.