Advance Search

2010 Vol. 26, No. 9

Display Method:
2010, 26(9): 1089-1090. doi: 10.11847/zgggws2010-26-09-01
Abstract(1172) HTML (196) PDF 210KB(47)
Abstract:
2010, 26(9): 1091-1091. doi: 10.11847/zgggws2010-26-09-02
Abstract(976) HTML (123)
Abstract:
2010, 26(9): 1092-1092. doi: 10.11847/zgggws2010-26-09-03
Abstract(1889) HTML (361) PDF 131KB(69)
Abstract:
2010, 26(9): 1093-1093. doi: 10.11847/zgggws2010-26-09-04
Abstract(1210) HTML (174) PDF 132KB(35)
Abstract:
2010, 26(9): 1094-1094. doi: 10.11847/zgggws2010-26-09-05
Abstract(918) HTML (196) PDF 159KB(56)
Abstract:
Predictive value of obesity indicators to diabetes risk
ZENG Wei, HUANG Xiao-bo, JIA Yong
2010, 26(9): 1095-1097. doi: 10.11847/zgggws2010-26-09-06
Abstract(1309) HTML (188) PDF 275KB(93)
Abstract:
Objective To evaluate the predictive values of body mass index(BMI),waist circum ference(WC),waist/hipratio(WHR),and waistheight ratio(WHRt) to diabetes risk.Methods Data came form the 2008 Chengdu Diabetes Survey,a population based cross sectional study,and 5205 residents aged 40-70 years were enrolled.Results WHRt showed positive correlation with WC(r=0.9384 in male,r=0.9526 in female,P < 0.05).WHR in male and WC in female showed low corre lation with height(r=0.0278 in male,r=0.029 4 in female,P > 0.05).WHR in male and WHtR in female were found to have the large stareas under the ROC curve relative to diabetes(c=0.641 5 in male,c=0.669 2 in female).Logistic reg ression analysis showed that WHR in male and WC in female were the best predictors related to obesity among the 4 de liberated indexes to diabetes risk(odds ratio[OR]=3.107 in male,OR=2.684 in femle).After controling the in teraction of 4 indexes,BMI combined with WC,WHR or WHRt could not improve the predictive value in the model.Conclusion The results suggest that WHR in male and WC in female are the best predictors related to obesity among the 4 evaluation indicators.The best cut off points of WHR in male and WC in female are 0.90 and 83 centmieter,respectively,and the application of the predictors in defferent a reaor population needs to be researched.
2010, 26(9): 1097-1098. doi: 10.11847/zgggws2010-26-09-07
Abstract(1006) HTML (191) PDF 184KB(45)
Abstract:
Analysis of influential factors for abnormal glucose metabolism with cumulative odds logistic regression
LIU Wen-xuan, LIU Dian-wu, LI Zhe
2010, 26(9): 1099-1101. doi: 10.11847/zgggws2010-26-09-08
Abstract(1371) HTML (198) PDF 287KB(40)
Abstract:
Objective To explore main influential factors for abnormal glucose metabolism and to provide scientific evidence for conducting diabete smellitus type 2(T2DM) prevention programe.Methods A sample of 3212 community residents aged over 40years was chosen from Baoding city with randomcluster sampling.The residents completed a question naire and received physical examination and laboratory test.The cumulative loggstic regression was used in the data analysis.Results DM family history,waist circum ference,trigly ceride,waisthip ratio,engaged in technical work,systolic blood pressrue,age,body mass index,ill temperorirr itability,consumption of fruit,gender,reproductive history of fetal macro somia(only to female) were significantly associated with the progress of abnormal glucose metabolism.Among them,female and a high consumption of fruit were protective factors,the others were risk factors.Conclusion A bnormal glucose metabolism is related to many factors.T2DM could be prevented by adjusting diet,taking physical exercise,controling weight,blood pressure and serum lipid,keeping mental health,and stopping smoking and a lcohol drinking.
Influence factors of gestational diabetes mellitus in Shenyang, a case-control study
TIAN Yu, YANG Liu, SHI Jing-pu
2010, 26(9): 1101-1102. doi: 10.11847/zgggws2010-26-09-09
Abstract(1300) HTML (157)
Abstract:
Objective To investigate the influential factors of gestational diabetes mellitus(GMD),and to provide evidence for etiologica l study of GDM.Methods Seventy four GDM patients were chosen through screening in Maternal and Child Health Unita s case group,and 76 healthy pregnant women were chosen at the same unit and same period as control group.Results Based on the analysis,GDM was probably a ssociated with obesity in pregnancy(odds ratio[OR]=15.139,95% confidence interval[CI]=2.910-78.765),body mass index before pregnancy(OR=1.317,95% CI=1.094-1.584),DM family history(OR=4.531,95% CI=1.065-19.284) and quantity of fruit intaking during pregnancy(OR=0.472,95% CI=0.241-0.926).Conclusion Obesity in pregnancy,BMI before pregnancy and DM family history are probably risk factors for GDM,and the quantity of fruit intake during pregnancy may be a protective factor for GDM.
Relationship between expression of adiponectin mRNA in adipose and placenta tissue and gestational diabetes mellitus
LUO Yi-ping, ZHAO Yi-qiao, XUE Yao-ming
2010, 26(9): 1103-1104. doi: 10.11847/zgggws2010-26-09-10
Abstract(1084) HTML (162) PDF 242KB(56)
Abstract:
Objective To investigate the expression of adiponectin mRNA in adipose and placentatissue among normal pregnant women and gestational diabetes mellitus(GDM) patients in order to evaluate the role of adiponectin in GDM.Methods Reverse trascription polymerase chain reaction(RT-PCR) was used to detectad iponectin expression level in adipose and placenta of 60 GDM cases and 60 normal controls after caesarean section delivery.Results The expression of adiponectin was significantly decreased in the adipose tissue of GDM cases campared to that of the normal controls(1.05±0.12 vs 1.28±0.14,P < 0.01) Conclusion There sults prove that the a lteration of adiponectin mRNA expression in adipose play an important role in the patho genesis of GDM.
Knowledge of diabetes in residents of Yinchuan city
WANG Fang, YANG Zhen-gui, ZENG Cheng-hui
2010, 26(9): 1105-1106. doi: 10.11847/zgggws2010-26-09-11
Abstract(1176) HTML (206) PDF 199KB(38)
Abstract:
Objective To investigate the knowledge rate and health behavior of diabetes mellitus among residents of Yinchuan city for conducting health education and health promotion in communities.Methods With stratified random sampling,residents elder than 20 years in two communities of Yinchuan were selected and investigated.Results Atotal of 1.084 eligible questionnaires were collected with an anwser rate of 95.76%.Only 12.73% of respondents anwsered all six questions about diabetes correctly.There was nosignificant difference in diabetes knowledge rate between participants of different age.There were significant differences in effects of diet and phy sical excercise on diabetes between male and female(P=0.037).Conclusion The residents of Yingchuan city have low knowledge rate of diabetes and the situation needs to be improved.
Influential factors of diabetic nephropathy among type 2 diabetes patients
LIN Rong, WANG Chun-na, LIU Xin-min
2010, 26(9): 1107-1109. doi: 10.11847/zgggws2010-26-09-12
Abstract(1262) HTML (187)
Abstract:
Objective To explore the influencing factors of diabetic nephropathy.Methods A 1:1 sex and age matched case control study was conducted among 139 type 2 diabetic nephropathy(DN) patients.Information was collected through interview for demog raphic data,disease history,family disease history,die tetic habit,behavior pattern,social psychologic character,clinical data and laboratory data.Polymo rphism of methylenete trahydro folate(MTHFR) gene was assessed with polymerase chain reaction(PCR) and restriction fragment length polymorphism(RFLP).The effects of risk factors on DN in type 2 diabetes patients and the potential interaction between the risk factors were analyzed with conditional logistic regression model.Results Univariate conditional logistic regression analysis showed that 667Ta llele of MTHFR gene,long diabetes history,diabetic retinopathy,lower ratio of albumm/globulin(A/G),higher fibrinogen were associated with higher risk of DN incident.Higher education,more exercise,control of sugar intake,higher vegetable in take after DM incident were associated with lower risk of DN incident.The interaction existed between duration of DM and geno type,vegetable in take and exercise,vege table in take after DM.Conclusion The incidene of DN of type 2 diabetes patient is associdted with multiplerisk factors.
Relationship between body mass index, waist circumference, waist to hip ratio, waist to height ratio and type 2 diabetes mellitus: a three-year follow-up study in Nanjing
HONG Xin, LI Jie-quan, LIANG Ya-qiong
2010, 26(9): 1109-1111. doi: 10.11847/zgggws2010-26-09-13
Abstract(1341) HTML (244)
Abstract:
Objective To explore whether the increase of body mass index(BMI),waist circum ference(WC),waist to hip ratio(WHR),and waist to height ratio(WHRt) can predict the risk of developing type 2 diabetes mellitus(T2DM).Methods A population based cross sectional study was conducted among local residents above 35 years in three urban districts and one rural county in July 2004.The subjects who were non T2DM in the base line survey were selected for a follow up survey in July 2007.Multiva riate logistic regression analyisis and area under curve(AUC) of receiver operating characteristics(ROC) were used to evaluate the predictive value of BM,IWC,WHR and WHtR.Results Of 4058 subjects with out T2DM in ba seline survey,3031 partic ipated the follow up survey with a follow up rate of 74.7%.The three year cumulative incidence of diabetes was 2.4%.The risk of developing T2DM increased a long with BMI,WC,WHR,and WHtR.Among the fourindices,higher WC and WHRt ≧0.5 had a higher risk of developing T2DM.Data from ROC curve analysis showed that the AU Cof WHtR was the highest.Conclusion The increase of BMI,WC,W HR and WHRt could predict the higher risk of the development of T2DM.WHtR might be a smiple and most effective index of T2DM.
Relationship between obesity, dyslipidemia and type 2 diabetes
ZHANG Yang-dan, TANG Xiao-jun, LI Ge
2010, 26(9): 1112-1113. doi: 10.11847/zgggws2010-26-09-14
Abstract(1491) HTML (219) PDF 218KB(96)
Abstract:
Objective To study the relationship between obesity,dyslipidemia and type 2 diabetes.Methods Acase control study among 184 type 2 diabetes patients from 3 hospitals and 212 normal health examinees was conducted in Chongqing city during the period of Oct,2008 to Feb,2009.Results The body mass index(BMI),waisthip ratio(WHR),serum total cholesterol(TC),and triglyce ride(TG) of the case group were higher than those of the control group And the test demonstrated that the differences were statistically significant.The over weight and obesity rates(64.67%),the detection rates of high TC(27.17%) and high TG(50.00%) of the case group were higher than those of the control group with significant differences.The multifactor non conditional logistic regression analysis showed that hypercho lesterolemia,overweight and obesity were major risk factors for type 2 diabetes.Conclusion Obesity and dyslipidemia are related to the occurrence of type 2 diabetes.Controlling weight and a reasonable diet are important measures to preventtype 2 diabetes.
Dietary structure among patients with diabetes or impaired glucose regulation in Uygur
SHAO Jie, DING Hong, ZHE Wei
2010, 26(9): 1114-1115. doi: 10.11847/zgggws2010-26-09-15
Abstract(1128) HTML (148) PDF 207KB(40)
Abstract:
Objective To investigate the dietary intake among patients with diabetes mellitus(DM) or impaired glucose regulation(IGR) in Uygur communities,and to provide scientific basis for carrying out diet related education and intervention in communities.Methods With stratified cluster sampling method,a cross sectional study was conducted among 529 subjects older than 40 years in 6U ygur communities of Urumqi.Finally 191 people were selected as survey sample and divided into three groups according to the result of oralglucose to lerancetest:DM group,IGR group,and normal group.In formation and awareness on diet was collected by 24 hours dietary recalled method and questionnaire.Results The in takes of macronutrients,vitamins and minerals of IGR group were more than that of DM group.The in takes of vitam in A,riboflav in,vitam in C,calc ium were all in sufficient for the three groups.The lack of calcium was the most obvious.The in takes of phosphorus,iron and vitam in E were more than that of recomm ended level.Conclusion The die tary structure of DM patients is not reasonable.Nutrition education among DM patients is necessary.
Acute hyperlipidemia model induced by yolk and Trition-WR1339 in mice
FENG Ren-nan, GUO Fu-chuan, LI Ying
2010, 26(9): 1116-1117. doi: 10.11847/zgggws2010-26-09-16
Abstract(1076) HTML (203) PDF 222KB(50)
Abstract:
Objective To establish an acute hyperlipidemia model with yolk and TritionW R 1339 in mice.Methods A cuteanmial models of hyperlipidemia were established in mice with 75% yolk peritoneal in jection(mode lA) and 3% Trition WR 1339 solution caudal vein injection(model B),respective ly.Inadditio n,the effects of smivastatin treamtent on the two acute hyperlipidemia models were investigated.Results After the yolk peritoneal injection,serumtrig lyceride(TG),total chole sterol(TC),low density lipo prote in cholesterol(LDLC),and high density lipoprote in cholesterol(HDLC) of model A were 14.02,7.33,2.98,and 1.03 mmol/L,respectively,which were significantly higher than those of control group A(P < 0.01).After 3% Triton WR 1339 solution cauda lvein in jection,serum TG,TC,LDLC,and HDLC of model B were 16.87,8.71,3.83,and 1.27 mmol/L,respectively,which were significantly higher than those of control group B(P < 0.01).Smivastatin treatment to the mice in the two models significantly decreased serum TG,TC,LDLC,and HDLC.Conclusion The application of 75% yolk peritoneal in jection and 3% Triton WR 1339 solution caudalve in injection can induce acute hyperlip idemia in mice.
Effects of methionine on distribution of arsenicals and NO metabolism in brain of mice exposed to arsenite
ZHANG Jun, WANG Yan, ZHAO Feng-hong
2010, 26(9): 1117-1119. doi: 10.11847/zgggws2010-26-09-17
Abstract(1038) HTML (181) PDF 299KB(29)
Abstract:
Objective To explore the effects of exogenous methionine(Met) on distribution of a rsenicals and nitric oxide metabolism in the brain of mice exposed to arsenite in drinking water.Methods Forty mice were divided randomly into 5g roups with 8 mice in each group.The mice in the exper miental groups were exposed to drinking water contam inated with 50 mg/L arsenic for four consecutive weeks.At the fourth week,the mice treated intraper itoneally with Met at doses of 100,200,and 400 mg/kg·bwand saline solution separately.Twenty four hours afterce ssation of Metadm in istration,the mice were anaesthetized and dissected rapidly.Sam ples of blood and brain were collected mimediately for analysis of arsenic species.Levels of inorganic arsenic(Ais),monomethy larsenic acid(MMA) and dmiethy larsenic acid(DMA) were determ ined with HG AAS method.Activities of nitric oxide synthase(NOS) and the concentrations of nitricoxide(NO) were determined with kits purchased from Nanjing Jiancheng Co.Results Compared to those treated with saline solution,adm inistration of Met significantly decreased the levels of Ais,MMA and total arsenic levels(TAs) in the blood and the levels of Ais,DM A and TAs in the brain.A ctivities of NOS and the levels of NO in As exposure group were significantly lower than those of in the control.However administration of Metcouldame liorate these toxic effects and NO levels in the group treated with 400mg/kg·bw.Met were significantly higher than those in saline solution treated group.Conclusion Exogenous Met could promote methylation of a rsenic,there fore reduce arsenic levels in both blood and brain.Moreover,administration of exogenous methionine couldame liorate the effects of arsenic on NO metabo lism in the brain via decreasing arsenic burden.
Detection of multi-drug resistant Mycobacterium tuberculosis by combined gene-chip and its clinical application
CHEN Hong-bing, WU Li-xia, ZHANG Juan
2010, 26(9): 1119-1121. doi: 10.11847/zgggws2010-26-09-18
Abstract(1297) HTML (247) PDF 301KB(35)
Abstract:
Objective To develop a new genechip for rapid detection of multidrug resistant Myco bacterium tuberculosis(M TB) to streptomy cin(SM),ethamtubol(EMB),rifampin(RFP) and/or isoniazid(INH).Methods The resistances of 300 MTB clinical isolates to SM,EMB,RFP and INH were detected with gene chip.Results Among the 300 MTB clinical isolates,188 isolate swere SM sensitive,112 were SM resistant with most of mutation of aag to agg on rpsL 43 and 88; 251 isolates were EM B sensitive and 49 were EMB resistant with the most of mutation of atg to gtg on embB 306; 251 isolates were RFP sensitive,49 resistant,and the mutation were mostly aag to gag on ropB 516; 210 isolates were INH sensitive,90 resistant,and the mutation were mostly gtg to gag on KatG 315.The sensitivity and specificity of the four antibiotic resistance were 81.1% and 99%,60.0% and 100%,72.0% and 98%,75.0% and 99%,respectively.For the isolates with different results by gene chip and conventional method,gene sequencing was applied,and the results showed that genechip for mutation detection had high specificity.Conclusion The detection for SM,EMB,RFP and INH resistance of MT B by combined gene chip is rapid and specific and could be applied to fast clinic screening.
Effect of Jinkuishenqiwan and micronutrients on testosterone level and anti-oxidation in aging mice induced by D-galactose
FENG Lei, BAO Shu-jing, FENG Chen
2010, 26(9): 1122-1123. doi: 10.11847/zgggws2010-26-09-19
Abstract(1183) HTML (231) PDF 215KB(52)
Abstract:
Objective To observe the effects of Jinku ishenqiwan and micronutrients on testosterone level and antioxidation in aging mice induced by Dga lactose.Methods The aging mice were divided into blank control,model control and four treamtent groups.The treamtent groups were treated with different dose of Jingkuishenqwi an or/and medicaments,respectively,and the control group with distilled water for 6 weeks.The serumtesto sterone,the activity of glutathion peroxi dase(GSHPx) and superox ide dismutase(SOD) in blood,lung,liver,brain and heartwere tested.Results The contents of testosterone for model control and four treatment groups were 1.734±0.102,1.826±0.069,1842±0 082,1.883±1.080,and 2.005±0.120 ng/m L,and the activities of GSHPx were 381.61±24.63,448.67±20.52,480.76±16.87,489.71±19.39,and 511.02±21.16 U/m l,respectively; the contents of SOD in blood were 13347.86±1147.86,16603.16±727.64,18378.42±908.91,18787.70±1322.91,and 19878.67±1148.77 U/g Hb for the mice in the model control group and four treamtent groups.There were obvious differences in the indexes between blank control group and model control group and four treamtent groups(P < 0.05 for all).The contents of GSH Px and SOD in lung,liver,bra in and heart of treamtent groups were increased significantly compared to that of the blank control group(P < 005).Conclusion Jingku ishenqiwan increasesse rumtesto sterone,the activity of GSH Px and SOD in the blood and tissues significantly,and shows a better effect when combined with micronutrient.
Simultenous detection of HIV-1 genes with multi-nested RT-PCR assay
XIAO Xin, WU Shu-zhi, SU Qi-jian
2010, 26(9): 1124-1125. doi: 10.11847/zgggws2010-26-09-20
Abstract(1406) HTML (243) PDF 244KB(49)
Abstract:
Objective To developan easy and reliable multinested RT-PCR assay for diagnosis of HIV-1 infection.Methods Several sets of PCR prmiers were designed to develop a multine sted RT-PCR assay for amplification of gag,pol,and envgene of HIV-1 variants prevailing in Guangxi.To tally 118 HIV positive samples and 48 HIV negative samples were detected with the multi nested RT-PCR assay and the detection resultswere compared to that of Western blot.Results The lowe stlmiit of the multine sted RT-PCR a ssay was 250 copies/mL and the sensitivity,specificity,and repeatability was 96.6%,97.9%,and 98.3%,respectively.The consistency bewteen the multine sted RT PCRassay and We stern blot a ssay was 97.6%.Conclusion A multinested RT-PCR assay with high sensitivity,specificity,repeatability,and time saving was developed and could be applied for auxiliary diagnosis of HIV1 infection.
Detection of acute flaccid paralysis(AFP) cases and poliovirus in Shaanxi province
XU Yi, XIA Xue-qin, LIU Xi-zhen
2010, 26(9): 1126-1127. doi: 10.11847/zgggws2010-26-09-21
Abstract(1324) HTML (184) PDF 205KB(106)
Abstract:
Objective To analyze the epidemiological character of AFP cases and to detect poliovirus in Shaanxi province during 2003-2008 in order to increase the sensitivity of vaccine derived poliovirus(VDPVs) surve illance.Methods Epidemiological analysis was conducted in A FP cases with positive poliov irus detection and VP1 genomic sequencing technique was used to analyze the molecular character of vaccine variant polio viruses.Results From 2003 to 2008,29 poliov irus strains were isolated from AFP cases,and the number of type Ⅰ strains exceed to the summation of type and type Ⅱ strains.75.86% of cases were children.The main paralysis parts of body were single or both lower extremity and lmibs.Fourteen strains were indentified as vaccine variant poliovirus.The vaiant rate of VP1 sequence did not reach 1%.Among these cases,7 cases were not fully vaccinated children and 9 were under 2 years old.Conclusion VD-PV emergence and cVD-PVs circulation still possibly occur in course of polio eradication.It is important to keep high level surve illance and vaccination coverage.
Treatment intention of non-commumicable chronic disease among residents in rural communities
YANG Hua-jie, ZHOU Zhi-heng, WANG Jia-ji
2010, 26(9): 1128-1130. doi: 10.11847/zgggws2010-26-09-22
Abstract(1297) HTML (183) PDF 284KB(238)
Abstract:
Objective To explore the treatment intention of non-communicable chronic disease(NCD) among residents in rural communities of Guangzhou and Zhong shan city for comprehensive prevention and cure of NCD.Methods To tally 278 rural residents from a town of Zhong shan and 20264 from a district of Guang zhou were selected randomly and investigated with a questionnaire.The treamtent intention of NCD and its main influencing factors among the subjects were analyzed.Results The prevalence rate of the non communicable chronic diseases among the residents of Guangzhou and Zhong shan were 12.43% and 9.43%,respectively.In Zhong shan,65.7% of the residents chose the district hospital or the second level hospital for the treamtent and the figure in Guang zhou was 49.2%.The proportion of the residents who chose the third level general hospital or specialized hospital for the treamtent of NCD in Guangzhou(25.9%) was obviously higher than that of in Zhong shan(3.9%,P < 0.05).The propor tions of residents choosing community health center for treamtent of NCD in the two cities were unive rsally low(29.1% and 14.6%) and the proportions of choosing hospital inenterprise or government institution,health care station and other medical institution for treatment were relatively low(1.3% and 10.2% in the two cities).Logisticreg ression analysis showed that age(odds ratio[OR]=1.138),occupation(OR=1.050),physical exercise(OR=0.508) and healthcare knowledge(OR=1.825) were prmiary influencing factors to the treamtent intention of NCD among the residents in rural communities in Guangzhou and Zhongshan city.Conclusion The treamtent intention of NCD among residents in rural communities is not high.Much more work should be done to guideruralre sidentswith NCD to take the treamtent in community health service organization,and to develop standardized management regulation for treamtent of NCD in community.
Risk factors of injury among greenhouse farmers
CUI Ning, WU Cui-zhen, HAN Xiao-chun
2010, 26(9): 1130-1131. doi: 10.11847/zgggws2010-26-09-23
Abstract(1170) HTML (158) PDF 217KB(34)
Abstract:
Objective To explore the prevalence of injury and related factors among greenhouse farmers in Shouguang city,and to provide evidence for effective in tervention.Methods With random sampling,1 018 farmers from 11 towns of Shouguang city were selected and investigated.Occupational injury in previous 12 months and related risk factors were examined.Univariate analysis and multivariate unconditionallo gistic regression analysis were abopted in the analysis.Results The inc idence of occupation related in jury was 71.9%.Physiology condition,psychologic status,social factor,and environment factor were closely related to the occurrence of occupational in jury.Multivariate study showed that health condition(odds ratio[OR]=0.304,95% confidence in terval[CI]=0.173-0.534),greenhouse temperature in summer(OR=0.875,95% CI=0.792-0.966) and in winter(OR=1.245,95% CI=1.124-1.379),humidity in summer(OR=0.942,95% CI=0.914-0.972),width of the greenhouse(OR=1.238,95% CI=1.005-1.525),length of the reenhouse(OR=1.021,95%CI=1.003-1.040),area of the greenhouse(OR=0.355,95% CI=0.181-0.694),height of the green house(OR=0.808,95% CI=0.673-0.969),and wall thickness of the greenho use(OR=0.995,95% CI=0.991-0.998) were related to the occurrence of the in jury.Conclusion The occurrence of in jury related to greenhouse work was correlated to envionmental factors.Effective measurements should be developed to reduce risk behaviors of occupation related injury.
Investigation on diet among college students in a university of Wuhan
HUANG Wan-qi, SUN Yan-feng, ZHOU Dun-jin
2010, 26(9): 1132-1133. doi: 10.11847/zgggws2010-26-09-24
Abstract(1546) HTML (286) PDF 209KB(85)
Abstract:
Objective To know the die tary structure and nutrien tintake among college student,s and to provide scientific basis for nutritional guidance to college students.Methods To tally 742 students were selected with random cluste red sampling in Wuhan Poly technic Univer sity on October 2009.A self designed food frequency questionnaire(FFQ) was used in dietary nutrition investig ation among the students.Results Pearson correlation analysis indicated that all of nutrients were positively correlated.Collage students dietary structure basically conformed to the die tary pagoda,and there was a significant difference between male and female in cereal intake(t=26.202,P=0.000).The average intake of energy,prote in,fat,carbohydrate,vitam in A,vitam in C,vitam in E,phosphorus,iron and zinc per day reached the recomm endednutrient in take(RNI) levels,and the ave rage intake of aneur in,ovoflav in,calcium and dietary fibre perday were below the the RNI levels(78.6-91.7%),but the average intake of cholesterol per day was over the a llow able lmiit of national standard(300 mg).There was no significant diffe rence among the nutrients intake in relation to sex,subjects in college(P > 0.05).Conclusion The college students nutritional status is good.The food frequency questionnaire might be an ideal die tary asse ssment in strument for college students.
Relationship between BMI, WHR and hyperlipidemia in female college faculty
ZHU Ming-yu, CUI Ling-ling, CHEN Mu-hua
2010, 26(9): 1134-1136. doi: 10.11847/zgggws2010-26-09-25
Abstract(1149) HTML (192) PDF 277KB(30)
Abstract:
Objective To understand the prevalence of hyperlip idemia and the level of blood fat in female college faculty and to investigate the relationship between body mass index(BMI),waist to hip ratio(WHR) and hyper lipidemia.Methods We analyzed the data of physica lexamination of 2098 female college staff in 2008 and used logistic regression in analysis of risk factor.Results The overall prevalence of hyper lipidemia was 40.1%.With the increase of age,the blood fat level was gradually increased(P < 0.01).Compared to the people with normal BMI and WHR,the blood fat levels of over weight and obesity were significantly increased(P < 0.01).The proportion of hypertrig lycer idemia was 59.10% among the staff under the age of 35 years and 33.6% among the staff above 55 years old.The proportion of hypertrigly ceridemia decrea sed with the increment of age but the proportion of hyperchole sterolemia and combined hyperlip idemia increa sed with age.Multiple logistic regression analysis showed that age,BM,IWHR were independent risk factors for hype rlipidemia,with the relative risks of 1.059(95% confidence interval[CI]=1.051-1.067),1.147(95%CI=1.106-1.189),45.526(95%CI=4.581-452.433),respectively.Conclusion The age,BMI and WHR are closely related with hyper lipidemia.
Anti-proliferative effect of δ-tocotrienol on colorectal cancer cells
LI Da-ming, DI Jin-ru, WANG Chun-hua
2010, 26(9): 1136-1137. doi: 10.11847/zgggws2010-26-09-26
Abstract(996) HTML (130) PDF 239KB(67)
Abstract:
Objective To investigate inhibitory effect of δ-tocotrienol on prolife ration of colorectal cancer(CRC) SW 620 cells.Methods SW620 cells we retreated with different concentration of δ-tocotrienol(0,20,30,40,60 mol/L).Methyl thiazolylte trazolium(MTT) method was adopted for the detection of cell growth and real time reverse transcription polymerase cha in reaction(RT PCR) for the detection of corre lated mRNA expression.The contents of GSK-3β,APC,and Ax in mRNA were detected.Results δ-tocotrienol inhibited proliferation of CRC-SW 620 cells.After treated with δ-tocotrienol for 24 hr,the survival rate of the cells was decreased by 14.4% to 88.8%.The RT PCR study showed that δ-tocotrienol promoted the expression of Ax in mRNA(F=6.598,P < 0.05),but not the GSK-3β mRNA and APC mRNA(P >0.05).Conclusion δ-tocotrienol can inhibit SW 620 cells proliferation.Its inhibitory mechanism correlates possibly with the adjusting of the associated expression of Ax in mRN A.
Health status and its related factors among Three Gorges migrants in Shandong province
QIN Jing-zhu, YUAN Chang-hai, LIU Bao-feng
2010, 26(9): 1138-1139. doi: 10.11847/zgggws2010-26-09-27
Abstract(1430) HTML (179) PDF 211KB(48)
Abstract:
Objective To explore health status of Three Gorgesmigrants in Shandong province,and to analyze related factors of health status.Methods Totally 617 Three Gorges migrants and 663 local residents were selected using stratified cluster samping method form 4 regions in Shandong province.Faceoface in terview was carried outwith a self made questionnaire.SPSS16.0 was used in analysis.Results The two weeks sickness prevalence among the migrants was 33.9%;the prevalence of chronic diseases was 35.0%,both of which were higher than those of local residentswith significant difference(P<0.05).Age,occupation,living adaptation,the degree of satisfaction with personal income were related to two week disease preva lence(P<0.01).Age,living adaptation,the degree of satisfaction to personal income were related to chronic diseases(P<0.05).Conclusion The health status of the Three Gorgesmigrants is poor.We should provide appropriate health service based on the need of the migrants in order to improve the irhealth status.
Reproductive toxicity and its mechanism of formaldehyde and benzene in mice
YANG Shuang-bo, KUANG Yi-le, LI Chun-Ying
2010, 26(9): 1140-1142. doi: 10.11847/zgggws2010-26-09-28
Abstract(1110) HTML (151) PDF 295KB(40)
Abstract:
Objective To explore the joint reproductivetoxi city and its mechanism of benzene and form a ldehyde in male mice.Methods To tally 108 Kunming male mice were randomly divided in to 9 groups according to 3×3 factorial design.Different dose of form a ldehyde(0,5,10 mg/kg),benzene(0,100,400 mg/kg),and both form a ldehyde and benzene were separately given to the mice by gastric gavage for 5 successive days.A fter 35 days,the mice were sacrificed and their testicular organ coefficient,spermmortality,malformation rate,testicular morphosis changes,testicular cell apoptosis were observed and the activity of Na+-ATP ase and Ca2+-Mg2+-ATP ase,the content of Ca2+ and total antio xidant capacity in testicle were detected.Results The sperm mortality and malformation rate and testicular apoptosis rate in the mice of the highest dose of formaldehy de and benzene combination group were 18.56±2.83,22.03±1.35,9.76±0.76%,respectively,with signifiant increase compared to that of the control(P<0.01).The testicular morphosis was damaged,the activity of Ca2+-Mg2+-ATPase,Na+-K+-ATPase and the content of total antioxidant capacity were decreased;the content of Ca2+ was increased in testicles of the mice of treated groups.There was a significant dose effect relationship(P<0.01)compared with that of the control group.The joint toxic effect of benzene and form a ldehyde exhibited synergistic or additive effect.Conclusion Form a ldehyde and benzene have synergistic or additive toxic effect on reproductive system of male mouse.The mechanism may be related to the inhibition of the A T Pase activity,the impact of total antio xidant capacity,and the disbalance of Ca2+ in mouse testicle.
Effects of dietary fiber on elimination of manganese in rats
YAN Wei, WANG Ke-yue, ZHOU Xi-lei
2010, 26(9): 1142-1143. doi: 10.11847/zgggws2010-26-09-29
Abstract(1247) HTML (186) PDF 211KB(26)
Abstract:
Objective To explore the effect of dietary fiber on the elimination of manganese and other trace elements.Methods Forty eight three week old male SD rats were randomly divided into sixgroups:control group(group A),group with supplement dietary fiber in diet(group B),Mn treatment group(group C),group with treatment of Mn+ 16% fiber in feed(group D),group of Mn+ 8% fiber in feed(group E),and group of Mn+ 4% fiber in feed(group F).The ratswere sacrificed after one month treatment.The change of weight,24 hour manganese content in stool,Mn,Cu,Fe,and Zn contents in brain,liver tissue and blood were determined.Results Compared with the group C(20.87%),24 hour discharge ratios of manganese of in stool of group D and E(29.44%,28.49%)were increased(P<0.05).Mn contents in bra in and liver tissue and blood in groug A(1.37 μg/g,1.78 μg/g,0.24 μg/ml),group D(1.62 μg/g,2.73 μg/g,0.35 μg/m l),group E(2.24 μg/g,2.54 μg/g,0.37 μg/m l),and group F(2.57 μg/g,2.70 μg/g,0.38 μg/ml)were decreased compared to group C(3.59 μg/g,3.22 μg/g,0.48 μg/ml; P<0.01).Cu,Fe and Zn contents in the of brain,liver tissues and blood of the rats had no significant difference among all groups(P>0.05).Compared with group A(35.99%),the change of weight ratio was decreased in groups C and F(25.98%,27.03%;P<0.05).Conclusion Die tary fiber may decrease manganese accumulation by increasing the discharge of Mn in stool.
Effect of marine collagen peptide on wound healing in rat after cesarean section
LIANG Rui, ZHANG Zhao-feng, ZHAO Ming
2010, 26(9): 1144-1145. doi: 10.11847/zgggws2010-26-09-30
Abstract(1120) HTML (206) PDF 216KB(57)
Abstract:
Objective To observe the effect of marine collagen peptide on wound healing in rat afterce sarean section.Methods Ninety six nearterm pregnant SD rats were delivered by cesarean section.Then,the rats were randomly divided into 4 group:sthree groups of marine collagen peptide at doses of 0.13,0.38,1.15 μg/kg·bw,and the saline group through gastric tube every day.Necropsy was performed on postope rative day of 7,14 and 21.The rats in each group were used to study the uter ine bursting pressure,skin tensile strength and hydroxypro line(Hyp)content of the scar.Results The contents of Hyp in skin scartissue for 1.15 μg/kg·bw group was significantly higher than that of the control group at three time points(P<0.05).The skin tensile strength and uterine bursting pressure of 1.15 μg/kg·bw group was significantly larger than that of in control group on the 7th and 21st day(P<0.05).The skinten sile strength of 0.38 μg/kg·bw group was significantly higher than that of in control group on the 21st day(P<0.305).Conclusion The marine collagen peptide can effectively promoted the wounding healing in rats after cesarean section.
Cloning and immunoreactivity of full-length cDNA sequence of Taenia saginata asiatica eukaryotic translation initiation factor 3 subunit
DAI Jia-lin, HUANG Jiang, LIAO Xing-jiang
2010, 26(9): 1146-1148. doi: 10.11847/zgggws2010-26-09-31
Abstract(951) HTML (146) PDF 326KB(35)
Abstract:
Objective To clone and express the gene named as eukaryotic translation initiation factor 3 subunit(EIFs3)of Taenia saginata a sitica and to analyze the mimunogenicity of its recombinant protein.Methods By online analysis of the gene at bioinfom aticswebsites,the gene from the Taenia saginata a siatica full length cDNA plasmid libratory was identi fied and its coding region sequence was analyzed.Then the coding region of the gene was amplified with PCR.The recombinant prote in was detected by sodium dodecyl sulfate polyacry lamide gelelec trophoresis(SDS PAGE)after being induced with isopro pyl thiogalacto side(IPTG)in Ecoli BL 21/DE3 and purified with NI IDA affinity chroma to graphy.In addition,the immuno reactivity of the purified recombinant prote ins was analyzed with We stern blotting.Results As demonstrated by PCR,double enzyme digestion and DNA sequencing of the recom binant plasmid was successively constructed.SDS PAGE results showed that the gene was expressed in E coli BL 21/DE3 and the recom binant prote in could react with Taenia a siatica andtae niarhynchus saginatus infected patientse rum,which indicated its mimuno reacticity.Conclusion The EIFs 3 of Taeniasaginata a sitica was cloned and expressed,and the purified protein has mimunogeni city.
Chronic fluorosis-induced renal tissue impairment in rats
WU Yi, ZHAO Qian, ZHANG Zi-gui
2010, 26(9): 1148-1149. doi: 10.11847/zgggws2010-26-09-32
Abstract(1055) HTML (153) PDF 290KB(56)
Abstract:
Objective To investigate the mechanism of renal impairment induced by fluoride.Methods The rats were freelyadmin istered with low,medium,and high concentration of NaF.The rats were sacrificed 9 months after the treatment,and the oxidation level and nuclear factor B(NFB)expression level in kidney were determined.Results The malondial dehyde contents and the activity of glutathione peroxidase of low dose and high dose groups were obviously higher than that of the control group(P<0.05).There was a non significant downward trend inactivity of superoxide dismutase(P>0.05).The NFB expression level increased with the exposure dose compared with that of the control group with an obvious increase in the high dose group(P<0.05).Conclusion Chronic fluorosis can causeoxidative damage of kidney tissue.
2010, 26(9): 1150-1151. doi: 10.11847/zgggws2010-26-09-33
Abstract(1309) HTML (224) PDF 242KB(30)
Abstract:
Bioinformatics analysis of calcineurin B gene in Taenia saginata
LUO Qiang, DAI Jia-lin, HUANG Jiang
2010, 26(9): 1151-1152. doi: 10.11847/zgggws2010-26-09-34
Abstract(1044) HTML (179) PDF 228KB(24)
Abstract:
Objective To analyze and predict the structure and characteristics of calcineurin Bin Taeniasa ginata(T.saginata)by bioinformatics analysis in order to guide the expermiental research for its biological function and application.Methods With analyzing tools of bioinformatics,the gene of calc ineur in B from the T.sa ginata full length cDNA plasmid library was recognized and the new gene was analyzed with bioinform atics and submitted to GeneBank.Results The gene is 510 bp in length with a coding region of 141-650 coding 170 aminoacids.It is a complete full length gene compared with that of the homolog ues in GenBank.The predicted molecular weight of calcineur in B gene is 19119.3Da.The prote in contains no transmem brane region and subce llular location sequence.It has four "EF-hand" calcium binding regions.Conclusion The cDNA sequence encoding calc ineur in B gene is screened from cDN A library of adult Taeniasa ginata by bioinformatical method.The structure and characteristics of Taeniasa ginata of the gene and prote in of calcineurin Bgene are obtained.
DNA damage of lymphocytes caused by chloropropanol in mice
LIU Yan-qun, WU Li-na, LE Ming
2010, 26(9): 1153-1154. doi: 10.11847/zgggws2010-26-09-35
Abstract(1062) HTML (186)
Abstract:
Objective To observe the damage of lymphocyte DNA induced by 1,3-dichloro-2-propanol(1,3 DC P) and 2-methy l-3-3-3 trichloro-1,2-propanedid(3-MCPD)in male mice with comet a ssay.Methods Fifty-six KM mice were divided randomly intofour groups:control group,low dose group(10 mg/kg),medium dose group(20mg/kg),and high dose group(40 m μg/kg).Peripheral blood of the mice was extracted after exposed for seven days.Comet a ssay was made with peripheral blood lymphocytes.Results The DNA dam age of lymphocytes in mice of the 1,3 DCP group was significantly higher than that of the control group.The rate of DNA damage were 22.90%,46.75% and 73.80%,respectively.Morevoer,DNA migration in high 1,3 DCP group was significantly higher than that of the low 1,3 DCP group.DNA migration was 8.056±2.291,9.378±3.658,and 2.3764±0.315,respectively.After the contamination,no significant difierence of DNA damage in mice were observed among the 3 MCPD groups(P>0.05).Conclusion 1,3 DCP has damage effect on DNA of peripheral bloodlymphocyte in male mice.The expermient of 3-M CPD does not show significant impacton DNA of lymphocytes in male mice.
Effect of acute lead exposure on Ca2+-CamkⅡ signal in hippocampus in rats
ZHANG You-xin, YE Li-ping, BAO Cui-fen
2010, 26(9): 1154-1155. doi: 10.11847/zgggws2010-26-09-36
Abstract(1124) HTML (188) PDF 231KB(33)
Abstract:
Objective To study the effect of acute lead exposure on Ca2+-CamkⅡ signal in rat hippo campus.Methods Hippocam palslices of 30 day sold heathy rat were collected and incubated with lead acetate(20 μmol/L)and non lead ace tate in artificial cerebro spinal fluid(ACSF)for the control,glutam ate,CaM KⅡ inhibitor(KN-93),and glutamic acid + lead ace tate group.Brain slices were collected after 30 min of culture.Western Blot was adopted to detect the activity of cyclic adeno sine-3'-5' mono phosphate response element binding prote in(CREB).Results CREB activity in the lead groupdecrea sed significantly with the time of lead exposure.CREB activity increa sed by 47% in glutam ate group and decreased by 20%,45% in KN-93 and glutamic acid+ lead acetate groups.There was no significant change in total CREB expression.Conclusion A cute lead poisoning may inhibit the activity of Ca MKⅡ and affect the downstream signaling molecules resulting in learning and memory damage.
Analysis on dynamic change of specific IgG antibody induced by schistosomiasis japonica soluble egg antigen with PROC MIXED procedure in SAS
XU Xiao-li, ZHANG Yuan-yuan, LIU Yun
2010, 26(9): 1156-1157. doi: 10.11847/zgggws2010-26-09-37
Abstract(1290) HTML (176)
Abstract:
Objective To analyze the serumlevel of soluble eggantigen(SEA)induced specific antibody IgG in or derto understand the dynamics of the IgG with PRO C MIX ED procedure in SAS.Methods Thirty female mice aged 6-8 weeks were divided into three groups:infection group(group A),the rapy group(group B),and reinfection grouop(group C).Ten cercarie/permice were used to infect the mice of group A and group B.Group B was treated with praziquantel at the dosage of 400 mg/kg 50 days after the infection.Group C was infected for the second time with 10 cercarie/per mice 70 days after the first infection.We used fiber micro tube to collect blood samples from angularvein of mice and preserve them at-20℃ frozen condition.A fterwards,we used enzyme linked mimuno sorbent a ssay(ELISA)to detect seruman tibody levels.Then we used PROC MIX ED procedure in SAS to analyze the fixed effect and ram dom effect of SEA induced spec ific an tibo dy IgG leve.l R esu lts There was no significant difference in IgG antibody at the time between 0 and 20 days a fter the infection.There was also no significant difference in IgG antibody at the time between 70 and 120 days a fter the infection.There were significant differences(P<0.0001)in IgG antibody at the time between 30 and 60 days after the infection.Conclusion The results present a dynamic change of specific antibody IgG induced by schisto somia sisjaponica SEA and coincides well with the biological observatio n.PRO CMIXED procedure could be adoted in the analysis of medical experiment data.
2010, 26(9): 1158-1159. doi: 10.11847/zgggws2010-26-09-38
Abstract(990) HTML (178)
Abstract:
Effects of different iodine intake on distribution and expression of sodium/iodide symporter in rats
YE Yan, LIN Lai-xiang, SUN Yi-na
2010, 26(9): 1159-1160. doi: 10.11847/zgggws2010-26-09-39
Abstract(1030) HTML (171) PDF 279KB(22)
Abstract:
Objective To observe the effects of differentiod ine intake on the dirtribution and expression of sodium/iodide symporter(NIS)prote in in rats with tissue microarray.Methods Wistar rats were randomly divided into three groups:low idoine(LI),normal iodine(NI)and high idoine(HI)based on the iodine levels of the diet.Three months later,urinary iod ine wasm easured and tissue microarray conta ining 48 spots of rattissue was prepared.Immmnohisto chemistry method was used to detect the distribution and expression of NIS prote in in different tissues.Results Compared with NI group,urinary iodine was considerably lower in LI group and higher in HI group,paralleling to the iriodine in takes.On the other hand,the different iodine intake induced changes of NIS expression in some tissues.The expression of NIS in thy roid follicuar epithelial cells was significantly higher in LI group than in NI and HI groups.On the contrary,NIS loacting at renal tubular epithelial cells in HI group streng thened obviously.NIS also distributed in gastric muco sa,in testina lmucosa,glandula ang ularis and ovaries with no obvious difference among the three groups.Conclusion The findings reveal that there are different expressions of NIS in several tissues of rats.Different iodine intake can cause changes of NIS expression in tissues,especially in thyrid and kidney.
Study on β-lactamases gene in extended-spectrum β-lactamases-producing Klebsiella pneumoniae
FU Yong-mei, ZHANG Yong-biao, XI Yun
2010, 26(9): 1161-1163. doi: 10.11847/zgggws2010-26-09-40
Abstract(1097) HTML (200) PDF 381KB(34)
Abstract:
Objective To investig ate the prevalance of lactamases(bla)gene in extended spectrum lactamases(ESBLs)producing Klebsiella pneumoniae(K.pneumoniae)clinicaliso lates.Methods ESBLs were detected with phenotypic screening and confirm a tory tests based on the Clinical and Labo ratory Standards Institute(CLSI).bla(TEM),bla(SHV)and bla(CTX M)genes were am plified by PCR,and the PCR products were cloned and sequenced to identify the irgeno types.Results The positive rates of bla(TEM),bla(SHV)and bla(CTX M)genes were 23.9%(11/46),28.3%(13/46),and 93.5%(43/46),respectively,in 46 strains of ESBLs producing K.pneumoniae.The strains carrying one,two or three kinds of bla genes were 45.7%(21/46),43.5%(20/46),and 6.5%(3/46),respectively.bla(TEM)genes were TEM 1 and bla(SHV)genes were SHV 11,12,28 in all these strains.bla(CTXM)genes were CTXM 15,24,38 in part of these strains.Conclusion CTXM type is the predominant genotypes in ESBLs producing K.pneum oniae,followed by SHV type,some of these strains carry broad spectrumlactam a ses gene TEM 1 or SHV 11.Monitoring the prevalance of lactam a ses genes in ESBLs producing bacte ria contributes torational use of an tibiotics in clinic.
Expression of TIP30 protein in brain astrocytoma
LIU Shu-zhe, GAO Wei-min, TAN Yan-li
2010, 26(9): 1164-1165. doi: 10.11847/zgggws2010-26-09-41
Abstract(1082) HTML (183) PDF 211KB(29)
Abstract:
Objective To explo re putative roles of transactivator of transcricption interactive prote in 30(TIP30)in the initiation and progression of a strocytoma.Methods The expression of TIP30,proliferating cell nuclear an tigen labeling index(PCNA L I),microv ascular density(MVD),apoptosis index(AI)in 69 cases of astrocy tomas and 8 cases of normal braintissue were comparatively studied with immunohistochemical staining method and termindal deoxynuc leotidyl transfernase mediated nick end labeling(TUNEL).Results The expression of TIP30 in astrocy toma was lower than that of normal brain tissue and was negatively related to the histo logical grade of tumor(χ2=16 31,P<0.01).MVD and PCN ALI with positive expression of TIP30 in astrocy toma were significantly lower than that with negative expression(P<0.01,P<0.01).AI with positive expression of TIP30 in astrocy toma was significantly higher than that with negative expression(P<0.01).Conclusion The decrease in expression of TIP 30 may play an important role in the in itiation and prog ression of astrocy tom a by promotingang iogenesis,tumor cell hype rplasia and decreasing apoptosis.
Effects of leptin on expressions of ACC and AMPK in cultured rat adipocytes in vitro
LIU Li, LIU Ya-li, MA Shuang
2010, 26(9): 1166-1167. doi: 10.11847/zgggws2010-26-09-42
Abstract(993) HTML (196) PDF 293KB(34)
Abstract:
Objective To observe effects of leptin on acyl Co A carboxy lase(ACC)and adenosine mono phosphate activated prote in kinase(AM PK)1 mRN A expression in cultured ratadipocytes in vitro.Methods Epididymal adiposetis sues from 3 male Wistar rats were dige sted and adiposetissue derived stromal cells were separated and cultured for adipogenic differentiation.Differentiated cells were incubated with ratrecom binant leptin of 50 nmol/L for 6 hr and expressions of ACC and AMPKα1mRNA were measured with semi quantitive reverse transcript polymerase chain reaction(RT-PCR).Results Ratprimary adiposetissue derived stromal cells could differentiate to adipocytes.The expression of ACC mRNA of leptin treated adipo cytes(0.39±0.29)was significantly lower than that of control(0.75±0.50,P<0.05).However,no significant difference was observed on the expression of AM PKα1 mRN A between two groups(P>0.05).Conclusion Rat recom binant leptin caninhibit fatty acid synthesis of cultured rat adipocytes in vitro,which may be unrela ted to AMPKα1.
2010, 26(9): 1168-1169. doi: 10.11847/zgggws2010-26-09-43
Abstract(963) HTML (195) PDF 217KB(27)
Abstract:
Effect of lead exposure on expressions of three subtypes neural cell adhesion molecule
YE Liu-qing, REN Tie-ling, LIU Tao
2010, 26(9): 1169-1170. doi: 10.11847/zgggws2010-26-09-44
Abstract(1177) HTML (177) PDF 215KB(29)
Abstract:
Objective To study the impact of lead exposure on the expressions of three main subtypes of neural cell adhesion molecule(NCAM).Methods NCAM 120,NCAM 140 and NCAM 180 recom binant plasmids were transfected in to SHSY 5Y cells separately using cationic lipo some transient transfection.After identification with mimuno histochemistry and Western blot,the cells were cocultivated with 1 mol/L or 10 mol/L PbC l2 for 24 or 48 hours.Then the expressions of three subtypes of NCAM were detected with We sternblot.Results After lead exposure for 24 or 48 hours,the expressions of NCAM 120 and NCAM 140 were reduced in the cells compared to that of the negative control group.The AO ratios of N CAM 120 decreased from 0.580(24h r)and 0.225(48hr)to 0.154 and 0.017;while the AO ratios of NCAM 180 decreased from 0.086(24hr)and 0.062(48hr)to 0.042 and 0.046.However,for the cells transfected with NCAM 140,only in the group with 10 mol/L lead exposure,the AO ratios decreased from 1.049(24hr)and 1.027(48hr)to 0.836 and 0.868.Conclusion Lead could influence the expression of the three subtypes of NCAM NCAM 120,NCAM 140 and NCAM 180,and the expresion of NCAM 120 is a ffected mostseriously by lead exposure.
2010, 26(9): 1171-1172. doi: 10.11847/zgggws2010-26-09-45
Abstract(894) HTML (137) PDF 217KB(49)
Abstract:
Urinary iodine levels among residents over age of 30 years in Shenzhen city
SHANG Qing-gang, LIU Xiao-li, XU Jian
2010, 26(9): 1172-1173. doi: 10.11847/zgggws2010-26-09-46
Abstract(1085) HTML (193) PDF 208KB(28)
Abstract:
Objective To understand the status of iodine nutrition among residents over age of 30 years in Shenzhen city.Methods To tally 277 residents from two communities were selected with stratified cluster raendom sampling.The morning urinary iodine was determined.Results The range of urinary iodine was 12.0-1705.5μg/L and the median of urinaryio dine was 167.7 μg/L a,among the residents.The percentages of the residents with urinary iodine <50,50-99,100-199,200-300,and >300μg/L were 1.8%,16.6%,44.8%,23.1%,and 15.5%,respectively.There was no sign ificant difference in urinaryiod ine levels among all age groups.However there was a negative correlation between the urinaryiodine and age with a correlation coefficient of-0.142(P=0.018).The medians of urinaryiodine of all age groups were with in the reconmm ended adequate intake except for the group of 40-49 which was slightly higher.There was no significant difference in the medians of iodine between different gender.Conclusion The iodinenutrition of residents in Shenzhen was in good condition,but populations with low or high iodine still exist and the monitoring is needed.
Hypothyroidism and its risk factors among pregnant women
LI Jian-ning, ZHOU Li-li, ZHAO Ying-chun
2010, 26(9): 1174-1176. doi: 10.11847/zgggws2010-26-09-47
Abstract(1351) HTML (181)
Abstract:
Objective To determine the epidemiological character istics and risk factors of hypothy roidism among pregnan two men.Methods Thyroid function and urinary iodine of 2076 pregnant women and 266 women at gestational age were measured and ultrasound inspection of thyroid was conducted among the women.Results The prevalence of hypothyroidism of pregnan twomen and women at gestational age were 5 3% and 6.8%,respectively.The prevalence of hypothy roidism was 7.6% among the pregnant women with high urinary iodine,which was significantly higher than that of the adequateurinary iodine group and very high urinary iodine group(P=0.026).Positive thyroid peroxidase an tibody ratio of pregnant women in hypothy roidism group was 23.9%,which was significantly higher than those of the normal gro up(P=0.000).Multinomial lo gistic analysis showed that high urinary iodine and positive thyroid peroxidase antibody were in dependent risk factor of hypothy roidism with the oddsratio values of 221(95% confidence in te rval[CI]1.36-3.60)and 4.68(95% CI 2.74-7.98),respectively.Conclusion The autoimmune thyroiditis and iodine overdose are important factors and closely associated with hypothy roidism among pregnant women.
Fatigue status among medical staff in surgical departments
LIU Wei-song, TIAN Ye, WANG Xiao-dan
2010, 26(9): 1176-1177. doi: 10.11847/zgggws2010-26-09-48
Abstract(1345) HTML (256) PDF 210KB(40)
Abstract:
Objective To determine the prevalence and in fluence factors of fatigue among medical staff in surgical deparmtent and to provide scientific evidence for prevention of chronic fatigue syndrome.Methods Altog ether 647 doctors and nurses in surgical deparmtents of three first grade hospitals in Hainan province were selected for a cross sectional survey.All subjects were a ssessed with Fatigue Scale 14(FS 14)and a self administered questionnaire for general information.Results The mean score of FS 14 was 7.83±2.94.The score was significantly higher than that of control subjects.The difference in total fatig ue between different gender was not statistically significant.But the difference in mental fatigue scores was statistically significant between women and men.Multiple linear regression analysis showed that the influential factors of fatigue among the medical staff were without breakfa stfrequently,irregular die,two rking duration,education,night-shift ≥2 times a week,weekly work ing hours,and workplace.Conclusion The influence factors of fatigue among doctors and nurses in surgical department are complex.Therefore,comprehensive measures should be taken to prevent the illness.
2010, 26(9): 1178-1179. doi: 10.11847/zgggws2010-26-09-49
Abstract(1428) HTML (180) PDF 201KB(46)
Abstract:
2010, 26(9): 1179-1179. doi: 10.11847/zgggws2010-26-09-50
Abstract(1156) HTML (183)
Abstract:
2010, 26(9): 1180-1181. doi: 10.11847/zgggws2010-26-09-51
Abstract(1069) HTML (188)
Abstract:
Prevalence of perinatal congenital heart disease and its influencing factors: a case-control study
MEI Jin, WANG Hao
2010, 26(9): 1181-1182. doi: 10.11847/zgggws2010-26-09-52
Abstract(1319) HTML (170) PDF 206KB(51)
Abstract:
Objective To investigate the prevalence and inflcencing factors of perinatal congenital heart disease in Hangzhou city.Methods Retro spective review of the data on reported perinatal defects(birth defect register)from 2006 to 2008 in Hangzhou city was conducted and the preva lence of perina tal congenital heart disease were analyzed.Results To tally 459 cases of perinatal congenital heart disease were found and compared with 977 normal cases born at the same area during the same period.The proportion of different congenital heart disease was 19.39% for ventricularseptal defect,16.12% for atrial septal defect,14.38% for patent ductusarte riosus,12.20% for tetralogy of Fallot,2.40% for aorticste nosis,3.05% for pumlonary stenosis,and 32.36% for other types,respectively.The history of flu of the mother and home decoration during early pregnancy were promotion factors of congenital heart disea se;educational level of mother and folate supplem entation during early pregnancy were protective factors of congenital heart disease(P<0.05).Conclusion Perinatal congenital heart disease is associated with flu of mothe,rhome decoration,educational level of mothe,rand folate supple mentation during early pregnancy.
2010, 26(9): 1183-1184. doi: 10.11847/zgggws2010-26-09-53
Abstract(912) HTML (158) PDF 216KB(52)
Abstract:
Demand and evaluation on early intervention service in parents with child of autism
ZHANG Xin, LI Ai-yue
2010, 26(9): 1184-1185. doi: 10.11847/zgggws2010-26-09-54
Abstract(1043) HTML (160) PDF 219KB(48)
Abstract:
Objective To investigate the evaluation and related needs in parents with children of autism for early intervention service in Tianjin.Methods Overall 59 children with autism were recruited from 5 early intervention agencies in Tianjin.A self designed questionnaire was used to collect related information.Results The average score of the evaluation by the parents on the agencies were 81.07(standard deviation=10.73).The highest score was for "the responsibility and suffurance of the teachers" and the lowest score was for "the ratio of teachers and children".Statistically significant differences were observed in teaching enviroment,ability of teacher,effect of teaching and satisfaction between public agency and private agency(P<0.05).Conclusion The parents with children of autism are satisfied with the early intervention service in Tianjin.These findings must be taken into account in policy making to provide better and more support for the agencies.
2010, 26(9): 1186-1188. doi: 10.11847/zgggws2010-26-09-55
Abstract(1493) HTML (416) PDF 367KB(70)
Abstract:
2010, 26(9): 1188-1188. doi: 10.11847/zgggws2010-26-09-56
Abstract(1014) HTML (167) PDF 137KB(43)
Abstract:
2010, 26(9): 1189-1190. doi: 10.11847/zgggws2010-26-09-57
Abstract(1128) HTML (187) PDF 205KB(39)
Abstract:
2010, 26(9): 1190-1192. doi: 10.11847/zgggws2010-26-09-58
Abstract(1209) HTML (249) PDF 298KB(49)
Abstract:
Hospital visit status of outpatients for basic medical institutions in Gansu province
LIU Yuan-qiang, ZHANG Zhong-nan, MA Guang-bin
2010, 26(9): 1192-1193. doi: 10.11847/zgggws2010-26-09-59
Abstract(935) HTML (123) PDF 207KB(48)
Abstract:
Objective To study the requirements of rural residents on health appropriate technology in Gansu province.Methods With stratified cluster sampling method,1 673 outpatients from Gaolan and Linze counties were chosen to investigate the irhospital visit status.Results The main diseases of the outpatients were acute upper respira to rytract infection,unknown illnes sign or symptom,pharyngitis,laryng itis,ton sillitis and tracheitis,and acute or chronic gastroente ritis.The average cost of each clinic visit was 34.93±2.84,31.41±2.47,and 17.15±2.21 RMB Yuan for hospitals of county level,town level,and village clinic,respectively.The reasons for the outpatients selecting medical in stitution at county or town level were short distance to the hospital,good medical skill,and better service a ttitude of the medical staff.Conclusion We should choose appropriate health technology based on the major disease and different medical demand of outpatients in ruralarea.
2010, 26(9): 1194-1195. doi: 10.11847/zgggws2010-26-09-60
Abstract(1061) HTML (172) PDF 193KB(30)
Abstract:
2010, 26(9): 1195-1195. doi: 10.11847/zgggws2010-26-09-61
Abstract(1222) HTML (180) PDF 121KB(26)
Abstract:
2010, 26(9): 1196-1196. doi: 10.11847/zgggws2010-26-09-62
Abstract(1168) HTML (200) PDF 130KB(214)
Abstract:
2010, 26(9): 1197-1198. doi: 10.11847/zgggws2010-26-09-63
Abstract(1268) HTML (157) PDF 199KB(44)
Abstract:
Cognition of evidence-based medicine among medical staff in Liaoning province
GUO Lei-lei, LIU Kun, ZHANG Lin
2010, 26(9): 1198-1199. doi: 10.11847/zgggws2010-26-09-64
Abstract(1223) HTML (193) PDF 210KB(23)
Abstract:
Objective To investigate the cognition of evidence based medicine(EBM)among medical staffs in Liao ning province and to explore the way to carry out EBM education.Methods A total of 1010 medical staff in 10 third class first grade hospitals in Liaoning province were investigated with a self designed que stionnaire o n personal info rm ation,related know ledge backg round,the cogn ition o f EBM and its influencing factors.Results There were 929 valid que stionnaires.Of all participants,77 persons(8.3%)knew the EBM very much;300 persons(323.3%)knew EBM a little better;426 persons(45.9%)knew EMB generally;126 persons(136%)didn't knew EBM.The main factors of impacting medical staffto gain EBM knowledge were education,occupation,professionaltitle,position,English proficiency,computer level,the frequency of using database,and reading medical literature.The proportions of the medical staffconsidering that EBM should be put into the diploma course,undergraduate crrriculum,and postgraduate course were 23.7%,58.0%,and 16.1%,respectively.Conclusion The cogniton of EBM is poor among medical staff and EBM education should be streng thened.
2010, 26(9): 1200-1201. doi: 10.11847/zgggws2010-26-09-65
Abstract(931) HTML (129) PDF 205KB(47)
Abstract:
Prevalence of iodine deficiency disorders in Bishan and Yunyang county of Chongqing city
SONG Yin-yin, MU Li-hong, LI Ge
2010, 26(9): 1201-1202. doi: 10.11847/zgggws2010-26-09-66
Abstract(1204) HTML (208)
Abstract:
Objective To understand the situation of iodine deficieney disorders(IDD)in Bishan and Yunyang country of Chongqing city in order to provide evidence for formulating prevention and control tactics.Methods With clusterran domsam ple method,urinary iodine,familial iodine salt use,rate of goite,rand in telligence quotient among the children aged 8-10 years were detected.Results To tally 571 urine sam ples were inspec ted.The urinary iodine median was 261.47μg/L.There were 33(5.78%)and 214(37.48%)children with urinary iodine median less than 100μg/L and more than 300μg/L.The iodine salt coverage rate,the qualified rate and qualified iodine salted ible rate was 99.64%,94.77%,and 94.42%,respectively,for 556 family table salt samples.Among 557 pupils,goiter was found in 60(10.36%)children by palpation and 53(9.15%)by Bultrasonog raphy.Conclusion The iodined salt use in the two counties is good,but there are still high level of urinary iodine and goiter rate than the national average.
2010, 26(9): 1203-1203. doi: 10.11847/zgggws2010-26-09-67
Abstract(1050) HTML (237)
Abstract:
2010, 26(9): 1204-1205. doi: 10.11847/zgggws2010-26-09-68
Abstract(960) HTML (192) PDF 220KB(30)
Abstract:
Correlation between life factors and Kaschin-Beck disease in Tibet residents
Ciyang, Xirao-ruodeng, Yangzong
2010, 26(9): 1205-1206. doi: 10.11847/zgggws2010-26-09-69
Abstract(1295) HTML (233) PDF 211KB(42)
Abstract:
Objective To explore the correlation between living factors and Kasch in Beck disease for porviding scientific infarmation on Kasch in Beck disease prevention.Methods Aself designed questionnaire was administered in 905 participants to obtain information on general condition and living factorsn with face to face interview.The partic ipants were from Lhundup,Medro Gongkar county of Lhasa municipa lity,and Sangri county of Lhoka Region.Results The household in come was negatively correlated with Kaschin Beck disease(r=-1.106,P<0.01);99.5% of the Kadchin Bced disease patients were illiterate or with low education.Spearmans rank analysis showed that alcohol drinkng was positively correlated with Kasch in Beck diseas(r=0.191,P<0.05).The highest rate of Kasch in Beck disease was observed in the residents drinking spring water.Conclusion There is a colse link between the occurrence of Kashin Beck disease and living factors.
2010, 26(9): 1207-1208. doi: 10.11847/zgggws2010-26-09-70
Abstract(1046) HTML (214) PDF 213KB(38)
Abstract:
Nosocomial infection among inpatients in general intensive care unit
WANG Shu-hui, ZHOU Cheng-chao, XU Ling-zhong
2010, 26(9): 1208-1209. doi: 10.11847/zgggws2010-26-09-71
Abstract(1149) HTML (204) PDF 214KB(59)
Abstract:
Objective To analyze the state of no socomial infection among inpatients in general in tensivecare unit(ICU),and to provide evidence for reducing hospital infection rate.Methods Using targetm onito ring method we compre hensively analyzed 1719 patients admitted into general ICU from Jan,2004 to Dec,2009.Results The infection rate of the patients was 30.95%.The main infection site was lower respiratory tract and the main bacteria were Gramnegative bacilli and Gram positive cocci.Gramstained negative bacilli were the main pathogens for no socomial infection,among which pseudomonas aeruginosa was on the top causing 13.61% of all infection cases,followed by Staphylococcus aureus causing 9.52% of all infection cases.Conclusion The patients in ICU are susceptible population of no socomial infection.Much more attention should be paid to targetmonitoring in ICU and intervention should be taken to reduce the prevalence of no socomial in fection.
2010, 26(9): 1210-1211. doi: 10.11847/zgggws2010-26-09-72
Abstract(963) HTML (180)
Abstract:
2010, 26(9): 1211-1212. doi: 10.11847/zgggws2010-26-09-73
Abstract(1338) HTML (243) PDF 204KB(28)
Abstract:
2010, 26(9): 1213-1214. doi: 10.11847/zgggws2010-26-09-74
Abstract(1590) HTML (240)
Abstract:
2010, 26(9): 1215-1216. doi: 10.11847/zgggws2010-26-09-75
Abstract(955) HTML (133)
Abstract: