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2012 Vol. 28, No. 12

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Comparison of HIV/AIDS prevalence among men who have sex with men between Suzhou and Yangzhou city
CHEN Xin, ZHU Ying-ying, HUAN Xi-ping
2012, 28(12): 1548-1552. doi: 10.11847/zgggws-2012-28-12-01
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Objective To compare the prevalence of human immunodeficiency virus/sexually transmitted disease(HIV/STD)as well as its related behaviors among men who have sex with men(MSM)between Suzhou and Yangzhou city and to provide evidence for specific intervention on the diseases.Methods Betw een April and July 2009,305 MSM in Suzhou city and 300 MSM in Nanjing city were investigated with a cross-sectional questionnaire survey.Blood samples were collected for HIV/STD test.Results There were significant differences in age,marital status,census register, education,occupation,monthly income,know ledge about AIDS among the MSM between the two cities.MSM in Suzhou were more likely to have multiple homosexual partners and have unprotected anal sex with men than MSM in Yangzhou. The prevalence rate of HIV infection in Suzhou was 9.2%(28/305)and the rate was 10.0%(30/300)in Yangzhou, without significant difference between the two cities.The prevalence rate of syphilis was 19.7%(60/305)in Suzhou, which was significantly lower than 31.3%(94/300)in Yangzhou.Conclusion There were some differences in demographics and risk behaviors between MSM in the two cities.Related measures of HIV/AIDS prevention and control should be adjusted to local conditions.
Evaluation of comprehensive prevention among HIV serodiscordant couples in Xinjiang
NI Ming-jian, CHEN Xue-ling, WANG Ji-liang
2012, 28(12): 1552-1554. doi: 10.11847/zgggws-2012-28-12-02
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Objective To explore suitable prevention method among human immunodeficiency virus(HIV)serodiscordant couples in Xinjiang for reducing the transmission risk among the group.Methods The serodiscordant couples were enrolled for an one year comprehensive intervention on prevention of HIV infection.Questionnaire survey was conducted at the beginning and the end of the intervention.At the same time,blood test was carried out once a quarter.Results The serodiscordant HIV positive rate in the couples was 2.17%(8/368).The number of sero-conversion was reduced quarterly during the follow up.The results from questionnaire survey showed that the know ledge rate of AIDS raised from 87.9%(471/536)to 91.0%(405/445);the rate of condom use in every sex in the previous year increased from 65.5%(311/475)to 81.8%(350/438);the rate of condom use in the latest sex increased from 80.6%(382/474)to 90.7%(359/396).The proportion of sex after drunk in the laest year was reduced from 18.8%(89/474)to 12.4%(49/396).The proportion of sex after drug use in the previous year declined from 11.4%(54/475)to 7.1%(28/396).The proportion of receiving health education,condom and HIV test regularly from health workers in the previous year increased.Conclusion The comprehensive intervention on prevention of HIV infection among serodiscordant couples in Xinjiang is very effective.
Nutritional assessment of children born to HIV infected mothers in Henan province
WANG Qi, HAN Ping, SUN Ding-yong
2012, 28(12): 1555-1558. doi: 10.11847/zgggws-2012-28-12-03
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Objective To evaluate the nutrition status of children born to human immunodeficiency virus(HIV)-infected mothers in rural area of Henan province.Methods Totally 160 children were included in the research.Z scores of weight for age,height for age,and w eight for height were calculated using WHO Anthro 2010 at birth and 1,3,6,9, 12,and 18 months of age.Z scores based on WHO standard were used to evaluate nutrition status of the children.Results Among the children,128 were HIV negative and 32 HIV positive.The incidence of underw eight in the HIV-negative children was lower than in HIV-positive children except for the children at the age of 3 months(P<0.05).The incidence of stunning in HIV-negative children was lower than in HIV-positive children at 6,12,and 18 months of age(P<0.05). The growth trend of Z values for age-related weight(WAZ)in HIV-negative children was better than in HIV-positive children(P<0.05)and the WAZ ranged from -0.59 to 0.79 for HIV-negative children and -1.41 to -0.04 for HIV-positive children.There were significant differences in Z values for age-related height(HAZ)at 9 and 18 months of age between two groups and HAZ ranged from -1.22 to -0.17 for HIV-negative children and -1.89 to -0.14 for HIV-positive children.The length and weight of the children at different months of age were lower than the normal standards significantly.Conclusion The incidence of malnutrition in the children born to HIV infected mothers in rural area of Henan province is high,especially in the HIV-positive children.
Pathogenic mycoplasmas infection and cellular immune function in males with HIV infection in Jiangsu province
CHENG Hao, QIAN Yan-hua, WU Jian-ru
2012, 28(12): 1558-1561. doi: 10.11847/zgggws-2012-28-12-04
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Objective To investigate the prevalence of Ureaplasma ruealyticum(Uu),Mycoplasma hominis(Mh),Myoplasma genitalium(Mg),Myoplasma fermentas(Mf),Myoplasma penetrans(Mpe),and Myoplasma pirum(Mpe) infection and to explore the relation between mycoplasma infection and immune function among males with human immunodeficiency virus(HIV)infection.Methods A questionnaire survey was conducted to collect related information.The first void urine was collected to detcet Uu,Mh,Mg,Mf,Mpe,Mpi and venous blood samples were collected to measure peripheral blood T lymphocytes and Th1/Th2 cytokines.EXCEL and SPSS 11.5 were adopted in data analysis.Results The infection rates of Uu,Mh,Mg,Mf,Mpe,Mpi among the males were 51.7,34.0,29.0,6.6,1.6,and 16.3%, respectively,and the ratio of the males with CD4+ cells count less than 200/μL was 21.8% and 50.9% of the male with the count of CD4+ cells between 200 and 500/μL.Among the males,the median level(ng/mL)of interleukin-2,interleukin-4,interleukin-6,interleukin-10,interferon-γ,and tumor necrosis factor-α(TNF-α)was 145.059,1.315,0.420, 2.405,4.083,7.157,respectively.The levels of CD3+,CD4+,CD8+,and TNF-α of the males with AIDS-associated mycoplasmas infection were significantly lower than those of without the infection(UCD3=2.86,P=0.004; UCD4=2.73,P=0.006;UCD8=2.40,P=0.017;UTNF-α=2.71,P=0.007).Conclusion Infections of 6 pathogenic mycoplasmas exist among males with HIV infection and the infection may be associated with host immune function and should be concerned in clinical practice.
Quality of life and its influential factors among AIDS patients in Zhumadian city
WANG Lu, YAN Rui-xue, GE Zeng
2012, 28(12): 1561-1564. doi: 10.11847/zgggws-2012-28-12-05
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Objective To study the quality of life and its influential factors among AIDS patients in Zhumadian city of Henan province,and to provide information for improving the quality of life of AIDS patients.Methods The World Health Organization's Quality of Life Instrument-Short Version(WHOQOL-BREF)(Chinese version)and a self-edited questionnaire were used to investigate 2 325 AIDS patients who were registered in "HIV serodiscordant family" and≥ 18 year-old and agreed to participate in a face-to-face interview.The score of quality of life of every domain and the risk factors of the score were analysed with logistic regression.Results AIDS patients' scores in the physical,psychological, social relations,and environmental domain were 12.94±1.94,12.38±1.93,13.82±2.44,and 12.39±1.92,respectively.Scores for self perceived quality of life and self perceived health status were 3.11±0.70 and 3.14±0.87.Multivariate analysis indicated that male,farmer,having AIDS related symptoms,and receiving antiviral treatment were risk factors associated with physical domain in the AIDS patients.Having sex with stable partners within the last 3 months,the transmission due to former plasma donation were protective factors for physical domain in the AIDS patients.Male,farmer, having AIDS related symptoms,having sexually transhitted disease(STD)related symptoms within the last one year were risk factors for psychological domain in the AIDS patients.Having sex with stable partners within the last 3 months,the transmission due to former plasma donation were protective factors for psychological field in the AIDS patients.Male,famer,having AIDS related symptoms were risk factors for social field in the AIDS patients.Having sex with stable partners within the last 3 months,the transmission due to former plasma donation and blood transfusion were protective factors for social field of AIDS patients.No factors were found to be significantly associated with environmental domain in the AIDS patients.Conclusion Gender,profession,whether or not having AIDS related symptoms,having STD related symptoms within the last one year,receiving antiviral treatment,having sex with stable partners within the last 3 months, and transmission routes were the main factors associated with the quality of life in the AIDS patients.
Prevalence of mood disorders among residents in Guangxi Zhuang Autono-mous Region
MA Zhen-yu, FENG Qi-ming, CHENG Qiang
2012, 28(12): 1565-1568. doi: 10.11847/zgggws-2012-28-12-06
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Objective To examine the prevalence of mood disorders among residents in Guangxi Zhuang Autonomous Region.Methods From July 2007 to December 2007,a total of 18 219 residents aged more than 15 years were randomly selected from 6 prefecture-level cities and 10 counties with stratified multi-stage cluster sampling.All subjects were investigated by face-to-face interviews.The Chinese Version of Composite International Diagnostic Interview(CI=DI 3.0)provided by the Chinese Center for Disease Control and Prevention was used as the screening tool and the diagnoses were made according to the International Classification of Diseases-10th edition.Results The general current prevalence and the general lifetime prevalence of mood disorders in the residents were 4.50‰ and 6.70‰.The risk factors of mood disorders were female gender,minority,and divorce based on logistic regression analyses.The general current prevalence was higher in the women than in the men(odds ratio[OR]=1.47,95% confidence interval[CI=:1.05-2.23)and higher in the minorities than in the Han(odds ratio[OR]=1.30,95% CI=:1.06-1.76).The divorsed individuals show ed higher current and lifetime prevalence of mood disorders.Conclusion Mood disorder is one of the most common mental disorders among the residents in Guangxi.The minority and divorsed individuals had a higher prevalence of mood disorder.Major deperession should be most concerned for the mood disorders among the residents.
Associations of physical activity and obesity with mental health among middle school students in Xuzhou,Jiangsu province
ZHANG Zhao-cheng, LU Qing-yun, WENG Ting-ting
2012, 28(12): 1568-1572. doi: 10.11847/zgggws-2012-28-12-07
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Objective To describe the associations of physical activity(PA)and obesity with mental health among middle school students in in Xuzhou,Jiangsu province.Methods A total of 5 730 boys and girls were selected from 10 middle schools with randomly clustering sampling and surveyed with a questionnaire and physical examination. Depressive symptoms of the students were assessed using Children's Depression Inventory(CDI)and their anxiety symptoms were assessed with the Screen for Child Anxiety Related Emotional Disorders(SCARED).Self-reported PA and other sociodemographic information were also assessed.Descriptive statistics and logistic regression model were used.Results The prevalence rate of obesity was 12.3%;the prevalence rate of depressive symptoms was 17.5%,and that of anxiety symptoms was 27.7%.Among the adolescents,self-reported rates of participating in moderate,vigorous, and insufficient PA were 13.6%,32.2%,and 63.2%,respectively.After adjusting for screen time,obesity and other potential confounders,insufficient PA was positively associated with depressive(odds ratio[OR]=1.18,95% confidence interval[CI=]:1.02-1.37)and anxiety symptoms(OR=1.15,95% CI:1.01-1.30).Insufficient PA as a moderate factor enhanced the association between obesity and depressive symptoms in junior high school students with the OR from 1.33 to 1.50.Conclusion Insufficient PA and obesity were closely related to the prevalence of mental health among middle school students in Xuzhou.Insufficient PA moderated the association between obesity and depressive symptoms in junior high school students.
Effects of overweight and obesity on children’s learning achievement and mental health
SHI Qian, WANG Li, WANG Fang-fang
2012, 28(12): 1573-1576. doi: 10.11847/zgggws-2012-28-12-08
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Objective To study the effects of overweight and obesity on children's learning achievement and mental health.Methods A total of 1 582 grade 3 students from 5 elementary schools in Taiyuan city were selected with random cluster sampling method.Primary and secondary mental health scales were performed for the studetns to evaluate their mental health status.Results The prevalence rate of overw eight and obesity in the students were 11.5% and 9.4%.The prevalence rates of overw eight and obesity were significantly higher in the boys(14.4% and 12.2%)than in the girls(8.5% and 6.5%).The learning achievement of normal children(188.95±10.76)was significantly better than that of obesity children(186.56±9.83)and the total score of mental health of normal children(466.83±52.07)was significantly better than that of obesity children(453.25±56.41).Multiple stepw ise regression analyses show ed that learning scores,father's educational level and mother's educational level were the main influence factors of children's learning achievement,and mother's educational level,gender and children's body mass index were the main influence factors of children's mental health.Conclusion The effect of obesity on children's learning achievement and mental health is significant.
Personality traits and parental rearing patterns among Mongolian and Han university students:a comparative study
ZHANG Jing, YAN Yan, XI Xue-song
2012, 28(12): 1576-1579. doi: 10.11847/zgggws-2012-28-12-09
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Objective To explore the relationship between personality traits and parental rearing patterns among Mongolian and Han university students and to provide basis for mental health education in the students.Methods A total of 722 Mongolian and Han college students were surveyed with Egna Minnen Barndoms Uppfostran(EMBU), Eysenck Personality Questionnaire(EPQ),and General Informational Questionnaire.Results All scores of EMBU factors for Han students were obviously higher than those of Mongolian students except for parents'emotional warmth(t=-7.14-3.03,P<0.001).In the Mongolian students,parents of girls were assigned higher scores in over-interference and over-protection than those of the boys(t=-4.06,P<0.001 or t=-2.40-2.21,P<0.05).In EMBU, there were no significant gender differences among the Han university students(t=-1.45-0.76,P>0.05).All scores of EPQ factors were statistically different betw een the Han and Mongolian students(t=-6.47-3.80,P<0.001).The Mongolian girls had a higher score in EPQ-psychoticism and the Han girls had a higher score in EPQ-neuroticism than boys(t=-4.10,P<0.001 or t=-2.05,P<0.05).Correlation and regression analyses revealed that the personality of both Han and Mongolian students exhibited close correlations with parental rearing patterns.Conclusion There are differences in personality traits and parental rearing patterns betw een Han and Mongolian students and parental rearing patterns have profound effects on personality among the students.
Sleep behavior and risk of uterine leiomyomata
ZHAI Shu-na, YAN Jia, LI Yan
2012, 28(12): 1580-1583. doi: 10.11847/zgggws-2012-28-12-10
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Objective To explore the association between sleep behavior and uterine leiomyomata(UL)risk in middle aged and elderly women.Methods A hospital-based retrospective study was conducted.Cases(n=385)were pathologically confirmed with UL and controls(n=385)were women with different diseases other than gynecologic diseases.All subjects were interviewed using a structured interviewer-administrated questionnaire and sleep behavior of the subjects were inquired with Pittsburgh Sleep Quality Index(PSQI)simutaneously.The odds ratio(OR) and the corre-sponding 95% confidence interval(CI)for UL and component scores of sleep behavior were estimated with multiple logistic regression model.Results The case group was significantly higher in grades of subjective sleep quality,habitual sleep efficiency,sleep disturbances,and overall PSQI scores than matched controls(P<0.05).Sleep quality,habitual sleep efficiency,sleep disturbances,and overall PSQI scores were associated with UL in univariate analysis.After adjustment for multiple potential confounders,compared with the controls,for the cases with scores≥2,sleep quality(OR=2.030,95% CI:1.261-3.181;P<0.001),habitual sleep efficiency(OR=2.437,95%CI:1.465-4.056;P<0.001),sleep disturbances(OR=1.564,95% CI:1.002-1.862;P=0.023),and overall PSQI scores were significantly related with the risk of UL.Compared with the women with the score<5,the women with the scores of 5-10 and >10 had approximately two times risk of UL(OR=1.833,95%CI:1.308-2.497;P=0.002;OR=1.923,95%CI:1.515-2.687;P=0.014).UL risk may increase with the increment of component score(P<0.05).Conclusion Sleeping may be a risk factor of UL and premenopausal women should raise the consciousness of the sleep influence on UL and do regularly gynecological examinations to prevent the disease.
Health status among left-behind children aged 1-4 years with both parents working out in rural Anhui province
CHENG Shi, ZHENG Hai-feng, PANG Pei
2012, 28(12): 1583-1586. doi: 10.11847/zgggws-2012-28-12-11
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Objective To assess physical and mental health status and the prevalences of nutrition disorder and accidental injury among left-behind children in rural Anhui for the improvement of health in the children.Methods Totally 424 left-behind children aged 1-4 years were selected from 12 villages in 2 counties of Anhui province,in which 212 were with both parents working out and 212 with both parents working at hometow n(control group).Physical development,peripheral blood hemoglobin,trace elements such as iron,zinc,magnesium,copper,iron,and calcium,urinary iodine,infant neuropsychological development,and accident injury were evaluated.Results The prevalences of malnutrition,nutritional anemia,zinc deficiency,iodine deficiency,calcium deficiency,and iron deficiency in the children with both parents working out were 4.7%,19.8%,46.2%,21.7%,4.7%,and 6.6%,significantly higher than those in the control group(0.9%,8.5%,34.4%,12.7%,0.9%,and 1.9%)(P<0.05 for all).The prevalences of mental retardation and edge intelligence were significant higher in left-behind children than those of control group(3.8% vs 1.9%, 20.8% vs 10.4%)(P<0.05).The prevalences of animal bite injury in the left-behind children was higher than in the controls,whereas,the prevalences of knife,sharp stab or cut injury,fish or other animal bone sticking in throat in the control group were higher than those in the children with both parents working out(P<0.05).Conclusion High rates of micronutrient deficiencies and neuropsychological development disorder among left-behind children were observed.The results suggest that attention to infant feeding and early education should be taken among guardians of rural left-behind children with both parents working out.
Effect of deltamethrin on gamma-glutamylcysteine synthetase activity and reduced glutathione content
LI Huang-yuan, WU Si-ying, SHI Nian
2012, 28(12): 1587-1589. doi: 10.11847/zgggws-2012-28-12-12
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Objective To explore the effect of deltamethrin(DM)on gamma-glutamylcysteine synthetase(γ-GCS) activity and reduced glutathione(GSH)content in PC12 cells.Methods PC12 cells were treated with 0,1,10,100 μmol/L DM for 6 hours or 12 hours,respectively.Cytotoxicity of DM on PC12 cells was measured by methyl thiazolyl tetrazolium(MTT)assay.The cytosolic fraction of PC12 cells was used to determine reduced glutathione(GSH)content andγ-GCS activity by reversed-phase high performance liquid chromatographic assay with o-phthalaldehyde pre-column derivatization.Results The survival rate of PC12 cells treated with 100μmol DM for 6 hours or 12 hours was lower than that of control group(P<0.01,P<0.001).In PC12 cells treated for 6 hours,there was no effect onγ-GCS activity and GSH content.In PC12 cells treated for 12 hours,there was no difference inγ-GCS activity and GSH content between low concentration and control group.Theγ-GCS activity of the high concentration group was significantly lower than that in the control group(P<0.05).Conclusion These findings demonstrate thatγ-GCS activity in PC12 cells could be inhibited by high concentration of DM treatment for a long time.
Cloning expression,purification and characterization of cashew nut allergen Anao2
LIU Fei-yan, JIA Meng-yang, LIU Zhi-gang
2012, 28(12): 1589-1591. doi: 10.11847/zgggws-2012-28-12-13
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Objective To clone and express the gene of the major allergen Anao2 from cashewnut.Methods The open reading frame(ORF)of Anao2 was cloned and inserted into the expression vector pMAL-c.The vector was transformed into Escherichia coli BL21(DE3)and the protein expression was induced by isopro-pyl-,-D-thiogalcatopyranoside(IPTG).Results The cloned ORF containing 1 332 bp and encoding 443 amino acids was authenticated to be Anao2.The recombinant Anao2 protein induced by IPTG was consistent with the actual value.Conclusion Cashew recombinant Anao2 protein is obtained.
Potective effect of geranylgeranylacetone on multiple organ injury in heatstroke rats
GAO Jun-tao, ZHAO Yong-qi, REN Kuang
2012, 28(12): 1592-1594. doi: 10.11847/zgggws-2012-28-12-14
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Objective To examine whether geranylgeranylacetone(GGA)could attenuate heat-induced multiple organ dysfunction in rats.Methods Fifty female anesthetized Wistar rats of clean degree were randomly divided into normothermic control group(NT,n=10),heatstroke model group(HS,n=10),lowdose GGA group(GGA1,n=10), moderate dose GGA group(GGA2,n=10),and high dose GGA group(GGA3,n=10).The model and GGA groups were orally administered vehicle(arabic gum)or GGA of 50 mg/kg,100 mg/kg,and 200 mg/kg,respectively,followed by the exposure to heat(43℃ for 70 min)before recovery at room temperature(RT,24℃).The normal control group rats were treated with vehicle and were kept at RT.A DKB-501S high precision water bath and circulation chamber of water were used to establish heatstrake model.The environment temperature was kept at 22-24℃ and the water temperature was kept at 45.0℃ for more than one hour,and then the temperature in the chamber was kept at 43.0℃.Results Compared with normothermic controls,all vehicle-treated heatstroke rats displayed hepatic and renal dysfunction,including increased levels of serum urea nitrogen,creatinine,aspartate aminotransferase,and alanine aminotransferase.The heat-stress response indicators were all significantly suppressed by GGA pretreatment.The pathological changes of lungs in heatstroke rats were alos attenuated by GGA pretreatment.Conclusion GGA preconditioning attenuates heat-induced multiple organ injury in rats.
Genetic subtypes and distribution of Seoul hanta virus in Liaoning province
GENG Ying-zhi, TIAN Jiang, LIU Yun
2012, 28(12): 1594-1596. doi: 10.11847/zgggws-2012-28-12-15
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Objective To investigate the genetic subtype and distribution of Seoul hahta virus in Liaoning province.Methods Rat lung samples and serum samples of the patients were collected in major epidemic areas and hantavirus antigens in the rat lungs were detected by indirect immunofluorescence assay.Partial M and S segments were amplified by nested reverse-transcription polymerase chain reaction(RT-PCR)using hantavirus specific primer and then sequenced, analyzed and compared with known sequences.The phyologenetic trees were constructed to analyze subtypes and geographical distribution.Results The Seoul hanta virus(SEOV)were carried by Rattus norvegicus in Liaoning province. The analytic results of partial M and S segments carried by rat lungs and patients indicated that the homology and variation of SEOV were high.The homology of nucleotides sequence was above 98.8% for samples from similar geographical areas,with a high homology for adjacent geographical areas.Conclusion The popular SEOV is S3 subtypes in Liaoning province and the gene subtypes distribution is single.
Prenatal exposure to PFOS alters individual genes NQO1 promoter methy-lation levels of liver in postnatal SD rats
SHEN Jie, XIA Wei, WAN Yan-jian
2012, 28(12): 1597-1599. doi: 10.11847/zgggws-2012-28-12-16
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Objective To examine the possibility of early epigenetic alteration in perfluorooctane sulphonate(PFOS)-exposed rat liver.Methods Pregnant Sprague-Dawley(SD)rats were exposed to PFOS at doses of 0.1,0.6, and 2.0 mg/kg/d and 0.05% Tween 80 as control by gavage from gestation day 2 to 21.The dams were allowed to give birth and liver samples from weaned(postnatal day 21)offspring rats were analyzed for individual genes such as NAD(P)H:quinone oxidoreductase(NQO1)and carnitine palmitoyltransferase 1A(CPT1A)promoter methylation level.Results In PFOS exposed weaned rats,compared to the control,methylation of critical CpG sites in NQO1 promoter was found up to 10% methylated in the livers of treated rats.Conclusion Early induced hypermethylation in critical cytosines within the NQO1 gene promoter region may be a significant biomarker of hepatic PFOS burden,though their direct role in PFOS induced-hepatotoxicity,including its potential carcinogenic action,needs further research.
Correlation between interleukin-1β-511C/T polymorphism and chronic heart failure
LI Ke-yan, LI Jin-zhao, QU Bao-ze
2012, 28(12): 1605-1607. doi: 10.11847/zgggws-2012-28-12-19
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Objective To explore the correlation between polymorphism at position-511C/T in the promotor region of interleukin-1β(IL-1β)and chronic heart failure.Methods A total of 160 subjects were selected and divided into chronic heart failure group and healthy group.Genomic DNA was extracted from 5ml vein blood and polymerase chain reaction-restriction fragment length polymorphism technique was applied to analyze the polymorphsim of IL-1β-511C/T.Results The results showed the frequency of T was higher in chronic heart failure group than in healthy group(56.25% vs 38.75%,P<0.05).Conclusion The polymorphism at position-511C/T in IL-1β is associated with chronic heart failure and T allele is the susceptibility genetic mark in some patients.
Relationship between polymorphisms of CYP1A1,CYP1B1 genes and susceptibility to recurrent abortion
ZHU Zhuang-yan, ZHAO Fu-xi, FU Xiao-min
2012, 28(12): 1607-1609. doi: 10.11847/zgggws-2012-28-12-20
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Objective To investigate the relationship between polymorphisms of CYP1A1 and CYP 1B1genes and the susceptibility to recurrent pregnancy loss(RPL).Methods The MspI polymorphism of CYP1A1 and the polymorphism in exon 3 codon 432(C-G of CYP1B1)were detected with the methods of polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)and allele specific-PCR(AS-PCR)in a case-control study including 81 cases of RPL and 98 healthy controls.Results There was no significant correlation between MspI polymorphism and RPL susceptibility(χ2=0.335,P=0.846).There were significant differences in the genotype distributions or allele frequencies of CYP1B1 L432V polymorphism between the two groups(χ2=7.467,P=0.024;χ2=9.129,P=0.003). Compared with wild-type C/C,the susceptibility of recurrent abortion with the genotypes of homozygotic mutation G/G and heterozygotic mutation C/G were increased by 2.620 and 1.954 times,respectively.Compared with allele C,the risk of recurrent abortion with allele G was increased by 2.038.Conclusion The gene polymorphism of CYP1B1 in exon 3 condon 432(C-G)might be a genetic risk factor of RPL and the allelic polymorphism of CYP1B1 L432V increases the risk of recurrent abortion.The results do not show that polymorphism of MspI is associated with the susceptibility of RPL.
Relationship between methylation capacity and skin lesions among patients chronically exposed to arsenic through drinking water
LI Xin, LI Bing, LIU Shi-yi
2012, 28(12): 1610-1612. doi: 10.11847/zgggws-2012-28-12-21
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To explore the relationship between methylation capacity and skin lesions among the patients chronically exposed to arsenic through drinking water.Methods Patients with arsenic related skin lesions were graded according to the Chinese Standard of Diagnosis for Endemic Arsenicosis.Levels of inorganic arsenic(iAs),monomethylated arsenic(MMA),dimethylated arsenic(DMA)in blood were detected by atomic absorption spectrophotometer with an arsenic speciation pretreatment system.iAs%,MMA%,and DMA% were calculated.Total arsenic(tAs),the first methylation ratio(FMR)and the second methylation ratio(SMR)were calculated as iAs+MMA+DMA,(MMA+DMA)/tAs and DMA/(MMA+DMA),respectively.Results There were no significant gender differences in the level of iAs,MMA,DMA,tAs,iAs%,MMA%,DMA%,FMR,and SMR.No significant difference in FMR was found among the mild,moderate and advanced group of skin lesions.SMR of moderate and advanced group(0.36±0.11, 0.37±0.08)were markedly lower than that of mild(0.48±0.11),while MMA% of moderate and advanced group(0.50±0.06,0.52±0.03)was significantly higher than that of mild(0.41±0.09).Significant negative correlation was found between SMR and the grade of skin lesions(r=-0.429,P<0.05).Conclusion The results suggest that lowsecondary arsenic methylation capacity and high MMA proportion are associated with the severity of arsenic-related skin lesion.
Addiction severity of drug dependent patients with methadone maintenance therapy in Dehong prefecture,Yunnan province
ZHANG Guang, LIU Hui, XUE Hui
2012, 28(12): 1612-1614. doi: 10.11847/zgggws-2012-28-12-22
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Objective To investigate the addiction severity of drug dependent patients with methadone maintenance therapy and its influential factors.Methods Totally 462 heroin dependent patients receiving methadone maintenance therapy in Dehong prefecture,Yunnan province were randomly selected with proportionally stratified sampling and interview ed with the measurement instrument of addiction severity index to measure their addiction severity in past one month and lifetime,and the influential factors related to addiction severity.Results Among the 462 patients,451 patients(97.6%)were male and 270 patients(58.44%)were married,with an average age of 37.80±9.13 years.The patients had a score of 1.02±0.42 for total addiction severity and and scores of 0.11±0.18,0.47±0.15,0.09±0.07, 0.08±0.13,0.01±0.03,0.23±0.14,and 0.05±0.11 for the seven functional domains of medical health,employment/self support,drug use,alcohol use,illegal activity,family relation,and psychiatric health,respectively.The addiction severity for the patients with different w ays of drug use was different in medical health,alcohol use,illegal activity,and family relations before receiving the treatment(F=5.450,P=0.001).The addiction severity for family/social relations of the patients with first use of drug at the age of younger than 16 years was significantly higher(P<0.05).The adition severity for drug use and alcohol abuse was different among patients with the drug use years of less than 5,5-10,and longer than 10 years before methadone maintenance therapy.Conclusion Addiction severity of heroin dependent patients adhering to methadone maintenance therapy could be decreased.
Prevalence of type 2 diabetes and its risk factors among residents in Zhangjiagang city
GU Zeng-hui, CHEN Xin-feng, LIU Xiang-lin
2012, 28(12): 1615-1618. doi: 10.11847/zgggws-2012-28-12-23
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Objective To investigate the prevalence of type 2 diabetes mellitus(T2DM)among residents in Zhangjiagang city and to explore risk factors of T2DM.Methods A multi-stage stratified sampling was used to select the residents above 18 years old and living in Zhangjiagang city more than 6 months.The data were collected with a questionnire survey,physical examination and laboratory test.Logistic regression was used to analyse risk factors related to T2DM.Results Totaly 8 175 residents(3 690 male and 4 485 female)were selected.The prevalence of T2DM was 6.86% and that of the impaired fasting glucose(IFG)was 10.39%.No significant difference in the prevalence between the male and female was observed(χ2=0.739,P>0.05).After adjusting for gender and education level,the results of logistic regression analyses showed that aging,family history of diabetes,blood pressure,central obesity,high total cholesterol(TC),high triglyceride(TG)were risk factors of T2DM,while exercise was a protective factor.Central obesity, high blood pressure,high TC and high TG were all significantly associated with T2DM in univariate analyses.Conclusion Aging,family diabetes mellitus history,central obesity,exercise,blood pressure,TC,and TG are main influencing factors of T2DM among residents of Zhangjiagang city.
Personality types and characteristics of serious physical assaulters in marriage
WU Lei, HONG Wei
2012, 28(12): 1618-1620. doi: 10.11847/zgggws-2012-28-12-24
Abstract(1452) HTML (200) PDF 329KB(48)
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Objective To explore the personality characteristics of spousal batterers who committed serious violence and to provide solid reference for prevention and management tactics on domestic violence.Methods Case-control design and stratified sampling methods were used.A total of 200 families of long-term residents in Xianyang city,Shaanxi province were surveyed with Conflict Tactics Scale and Personality Diagnosis Questionnaire(PDQ)4th vision.Independent sample t-test and chi-square analysis were used to examine the difference betw een the two groups.Results The PDQ total score(34.24±16.13 vs 27.05±19.56),scores in schizoid subtype(1.75±1.43 vs 1.17±1.08),schizotypal subtype(2.58±1.61 vs 1.89±1.66),antisocial subtype(3.28±4.17 vs 1.93±3.35),borderline subtype(3.33±1.81 vs 2.53±2.06),and dependent subtype(3.52±2.10 vs 2.52±2.20)of battlers group were significantly higher than those of control group(P<0.05).The number of people who reached the cutting-off point of antisocial dimension in battlers group was significantly greater than that in control group(χ2=5.49,P<0.05).Conclusion The mental health condition of serious spouse-batters was problematic with some negative personality characteristics.
Seasonal succession of ticks in small wild animals in Chengmai county of Hainan province
SUN Ding-wei, WANG Shan-qing, ZHAO Wei
2012, 28(12): 1620-1622. doi: 10.11847/zgggws-2012-28-12-25
Abstract(1316) HTML (207) PDF 517KB(48)
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Objective To examine the seasonal fluctuation of ticks in small wild animals in Chengmai county,Hainan province.Methods Ticks were collected from the body surface of small wild animals.Results From April 2009 to March 2010,totally 8 species of hosts were caught and all the ticks collected from these hosts were Ixodes granulatus with the totall parasitic index and rate of 2.57 and 38.98%(118/303),respectively.The peaks of the index were observed in April for total index and adult vector parasitic rate.While the peaks for larvae and nymphs(1.83 and 3.33)were observed in November and January.The highest value of total parasitic rate was in April(76.19%).Peaks of parasitic index and rate for Tupaia belangeri and Niviventer fulvescens were all in April,while two peaks for Rattus rattus hainanicus were recorded in April 2009 and January 2010.Parasitic index and rate of Tupaia belangeri was highest among the hosts(7.64 and 69.57%).Conclusion Ticks in Chengmai area are of seasonality with the most active month in April.The fittest host for I.granulatus is Tupaia belangeri.
Nutritional status of Tujia and Miao minority middle school students in Xiangxi autonomous prefecture
ZHANG Fu-lan, ZHANG Tian-cheng, WEN Li-zhong
2012, 28(12): 1623-1625. doi: 10.11847/zgggws-2012-28-12-26
Abstract(1092) HTML (182) PDF 330KB(57)
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Objective To examine the nutritional status of Tujia and Miao minority middle school students in Xiangxi Tujia and Miao autonomous prefecture for the improvement of minority students'nutritional status.Methods Totally 3 402 Tujia and Miao minority students were selected and their height and weight were measured and evaluated with the method of height with standard weight.Results In 2009,the malnutrition rate in the Tujia and Miao students was 2.32%(39/1680)and 1.51%(26/1722);the underw eight rate was 21.67%(364/1 680)and 18.82%(324/1 722); the overw eight rate was 7.02%(118/1 680)and 9.47%(163/1 722);the obesity rate was 3.27%(55/1 680)and 4.76%(82/1 722),respectively.The underw eight rate of Tujia students was oviously higher than than of Miao students; the overw eight and obesity rate of Miao students were obviously higher than those of Tujia students.The underw eight rate of female students was higher than that of male students obviously;the obesity rate of the male students was higher than that of the female students obviously.The malnutrition rate of the senior students was higher than that of the junior students and the junior students,especially the junior students in low grade had a higher obesity rate.Conclusion Underand over-nutrition exist in Tujia and Miao minority middle school students at the same time and measures should be taken in different groups according to their different nutritional status.
Epidemic characteristics and disease burden of injuries among community inhabitants in Nanning city
ZHOU Rong-jun, YAO Mei-hong, YANG Li
2012, 28(12): 1625-1627. doi: 10.11847/zgggws-2012-28-12-27
Abstract(1131) HTML (165) PDF 315KB(41)
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Objective To examine the epidemic characteristics and burden of injuries among community inhabitants in Nanning city.Methods A household questionnaire survey was used to collect information about the incidence,disease burden and major influencing factors of injuries among urban inhibitants selected with random cluster sampling.Results The overall incidence of injury was 28.34%.Falls(8.73%),blunt/sharp object injury(6.24%)and traffic injuries(3.92%)were the leading three injuries.Slippery roads(32.65%),knife/glass injury(51.43%),violation in driving(54.54%),water scald(63.16%),food poisoning(71.43%),animal bites(55.56%)were major factors for falls, blunt/sharp object injury,traffic injuries,burn/scald,poisoning,and animal injury.The total annual economic loss caused by injuries was 3 965.8 thousands RMB yuan.Conclusion The injuries result in heavy economic burden to the society and families in urban area of Nanning city and health education and intervention should be strengthened in the urban population.
Surveillance on mosquito vectors of dengue fever and dengue virus in Longgang district of Shenzhen city,2009-2011
ZHOU Jian-ming, WANG De-quan, LIN Lin
2012, 28(12): 1628-1630. doi: 10.11847/zgggws-2012-28-12-28
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Objective To explore the infection of dengue virus(DV)in mosquitoes and to determine the prevalence of serum anti-DV IgG antibody among healthy people in Longgang district of Shenzhen city for assessment of dengue fever epidemic risk.Methods Surveillance on the density and species of mosquito was conducted in 10 major subdistricts and 21 gymnasiums in Longgang district.The DV was identified with real-time reverse transcription PCR(RTPCR)and anti-DV IgG antibody level in healthy population was detected with enzyme-linked immunosorbent assay(ELISA).Results A total of 25 556 mosquitoes were collected from 2009 to 2011 and among the mosquitoes Aedes togoi and Aedes albopictus accounted for 2.45% and 1.60%,respectively.The indexes of mosquito and egg trap in schools(18.34%)and construction sites(11.29%)were above average value.The average mosquito density in hospitals(27.67 mosquitoes)was the highest among different sites.The real-time RT-PCR results showed that two positive samples were found and the maximum nucleotide homology with DV epidemic strains ofⅡsubtype was over 98%.The positive rate of the serum anti-DV IgG antibody of healthy population was 4.2%.Conclusion Although the density of Aedes mosquitoes was low,but the prevalence of viral-infection caused by mosquitoes was high.The results indicate that people in Longgang area are susceptible population for dengue virus and mosquito control and epidemic surveillance on imported cases should be strenthened.
Prevalent features of hepatitis E among residents in Wuhan from 2001 to 2010
KONG De-guang, YU Bin, LUO Tong-yong
2012, 28(12): 1631-1633. doi: 10.11847/zgggws-2012-28-12-29
Abstract(1118) HTML (153) PDF 321KB(60)
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Objective To analyze the incidence and epidemiological trend of hepatitis E(HE)among residents in Wuhan from 2001 to 2010.Methods Descriptive epidemiological and correlation analytical methods were used.Results Totally 5 011 cases of HE were reported in Wuhan from 2001 to 2010,with an average incidence of 6.20 per 100 000 and a downward trend(χ2=836.53,P<0.001).No obvious seasonal distribution was observed for the reported HE cases,but the incidence was relatively higher in winter and spring.The average incidence of HE was higher in the urban than in the rural(χ2=1 284.65,P<0.001).The incidence of HE increased with the increment of age(r=0.816,P< 0.001)and was the highest in the risidents aged 55 to 84 years,and higher in male than in female(χ2=848.01, P< 0.001).Among the all cases,retirees accounted for 28.46 percent and workers were 16.74 percent.Conclusion In recent years,the incidence of HE was high among the residents in Wuhan city and was higher in urban residents,male population,retirees,and the people aged over fifty.
Relationship between dietary structure and perimenopausal syndrome in women of Tai’an city
SUN Zheng, LIU Feng-yun, HAO Yan-qing
2012, 28(12): 1633-1635. doi: 10.11847/zgggws-2012-28-12-30
Abstract(815) HTML (121) PDF 321KB(42)
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Objective To explore perimenopausal women's dietary structure,the prevalence of menopause syndrome and its related factors.Methods Totally 862 female outpatients from 3 hospitals were selected.The patients were aged between 40 and 60 years,with uterus and one or two ovaries,and with menstrual changes or menopause syndrome symptoms.The menopausal symptoms were graded by improved Kupperman evaluation standard and the diet structure was evaluated with the standard of "The China Dietary Guidelines(2007)".Results The menarche age of the perimenopausal women was 14.8±1.9 years and the menostasis age was 49.2±4.8 years.There were 799(92.69%)patients having one or more menopause symptoms.Linear correlation analyses showed that improved Kupperman symptom score was correlated with menopause syndrome score.The dietary structure of the patients was not consistent with the recommended value of the Chinese balanced diet guide.Conclusion Most of the perimenopausal women's dietary structure was not rational.The relationship between dietary behavior and menopausal symptoms needs further research.
Hospitalization expenditure among cerebral infarction patients with New Cooperative Medical Scheme,2007-2010
YUAN Sha-sha, Clas Rehnberg, MENG Qing-yue
2012, 28(12): 1636-1639. doi: 10.11847/zgggws-2012-28-12-31
Abstract(1137) HTML (203) PDF 355KB(38)
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Objective To explore the changes and influencing factors of hospitalization expenditure among cerebral infarction patients from 2007 to 2010.Methods Retrospective analysis was used.The data on hospitalization expenditure for 5 319 cerebral infarction patients with New Cooperative Medical Scheme(NCMS)were extracted from NCMS information system in a county of Shandong province.Descriptive statistics and factor analysis were used to analyze different expenditure types.Results The average inpatient expenditure increased by 57.7%;medicine expenditure(63.23%) was the dominant part of the expenditure for all hospitals of different levels,followed by treatment(15.57%)and examination(12.28%).For the components of the medicine,the expenditure of western drug decreased from 78.56% in 2007 to 65.02% in 2010,while that of Chinese patent medicine increased from 20.96% in 2007 to 34.43% in 2010.Four factors were extracted by factor analysis,including "treatment,western medicine,examinations and materials" factor,Chinese patent medicine factor,Chinese herbs factor,and bed factor.Conclusion Western medicine,Chinese patent medicine, treatment and examination were four main components of the increase of hospitalization expenditure.Chinese patent medicine and examination were the items with higher increaed expenditure for hospitals at town level and western medicine for hospitals at county level and above.
2012, 28(12): 1639-1640. doi: 10.11847/zgggws-2012-28-12-32
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Detection of human calicivirus in different water samples from Guangzhou city
ZENG Ai-hua, JIN Xiao-bao, ZHU Jia-yong
2012, 28(12): 1641-1643. doi: 10.11847/zgggws-2012-28-12-33
Abstract(1065) HTML (157) PDF 325KB(41)
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Objective To assess the prevalence of human calicivirus(HuCV)contamination in different water samples from Guangzhou city.Methods Totally 268 tap water,715 mineral w ater,119 living sew age,and 53 pool water samples were collected.All the samples were detected with real-time fluorogenic quantitative PCR for HuCV(including GⅠ,GⅡNorw alk-like viruse[NLVs],and Sapora-like viruses[SLVs]).Results The positive rate of HuCV for the samples was 9.24%,11.32%,0,and 0,respectively;the positive rate of GⅠNLVs,GⅡNLVs,GⅠSLVs,and GⅡSLVs for domestic sewage samples was 2.52%,7.56%,0 and 0;the positive rate of GⅠ/GⅡNLVs and GⅠ/GⅡSLVs for pool water samples was 1.89%,9.43%,0,and 1.89%,respectively.GⅠNLVs and GⅡNLVs were detected in one domestic sewage sample;GⅡNLVs and SLVs contamination were detected in one pool water sample.Conclusion The drinking water in Guangzhou area is safe,but different genotypes of HuCV contamination exists in other water samples.
2012, 28(12): 1645-1648. doi: 10.11847/zgggws-2012-28-12-35
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2012, 28(12): 1648-1649. doi: 10.11847/zgggws-2012-28-12-36
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2012, 28(12): 1652-1654. doi: 10.11847/zgggws-2012-28-12-38
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2012, 28(12): 1655-1656. doi: 10.11847/zgggws-2012-28-12-39
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2012, 28(12): 1657-1658. doi: 10.11847/zgggws-2012-28-12-40
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2012, 28(12): 1658-1659. doi: 10.11847/zgggws-2012-28-12-41
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Electrochemical determination of diethylstilbestrol with carboxylic carbon nanotube modified electrode
YU Chun-mei, ZHOU Xiao-hui, JI Wan-yu
2012, 28(12): 1663-1665. doi: 10.11847/zgggws-2012-28-12-44
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Objective To establish a new method for the determination of octylphenol(OP)using modified electrode.Methods c-CNT-chitosan modified electrode was constructed by immobilizing carboxylic carbon nanotubes on the surface of chitosan modified glassy carbon electrode with a self-assembly method.The electrochemical behavior and determination of OP with modified electrode were investigated by cyclic voltammetry and differential pulse voltammetry in pH 6.0 phosphate buffer solution.Results In phosphate buffer solution at pH of 6.0,the calibration curve for OP determination could be obtained with the linear range of 8.33×10-8-2.0×10-5 mol/L and the estimated detection limit of 3.67×10-8 mol/L(S/N=3).The relative standard deviation was 3.6% for ten determinations of 3.0×10-6 mol/L OP using the same electrode.The recoveries ranged from 92.5%-106.5% for the samples detected.Conclusion The method is simple and sensivite and could be applied to the determination of OP in samples.
Subspecies typing of Vibrio parahaemolyticus in aquatic products with pulsed-field gel electrophoresis
ZHANG Xin, WANG Qi, ZHANG Hui-yuan
2012, 28(12): 1666-1668. doi: 10.11847/zgggws-2012-28-12-45
Abstract(1118) HTML (153) PDF 335KB(52)
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Objective To assess subspecies typing of Vibrio parahaemolyticus strains from aquatic products with pulsed-field gel electrophoresis(PEGE).Methods A total of 80 selected isolates obtained from aquatic products were characterized with PFGE method.Genomic DNA w as digested with restriction endonucleases NotI and SfiI.Results The isolates were grouped into 68 PFGE patterns by NotI and SfiI.Moreover,there were 70 PFGE patterns after compared with the results of the two restriction enzymes.The discrimination power of NotI was 78.8% and that of SfiI was 76.3%.Fourteen strains were from Canada four strain groups according to the Dice coefficient of 100%,thereinto,the Dice coefficient of four strains was 91.8% and the Dice coefficient of ten strains was 93.3%.Three strains form Norway and two strains from New Zealand belonged to 2 groups,with the Dice coefficient of 100%.There were no other same strains detected.Conclusion The results indicate that PFGE with both NotI and SfiI could be used in discriminant analysis for Vibrio parahaemolyticus,but NotI is more effective.The clustering analysis showes a clear disagreement among the strains and their sources.Furthermore,the strains with virulence genes are included in the same or contiguous clusters.
Evaluation on external quality control of laboraties for iodine deficiency disorders in Guangdong province
LIU Li-ping, YANG Tong, ZHONG Wen
2012, 28(12): 1668-1669. doi: 10.11847/zgggws-2012-28-12-46
Abstract(940) HTML (167) PDF 257KB(23)
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To evaluate the capability of laboratories for iodine deficiency disorders(IDD)for the improvement of the laboratories in Guangdong province.Methods The assessment results of external quality control for IDD laboratories in centers for disease control and prevention at provincial,municipal,and county level were analyzed and the capability in IDD detection and its influencing factors were explored.Results The rates of response for urine iodine detection were 100% for the laboratories at provincial and municipal level during the period of 2001-2011 and with an average qualification rate of 97.4% for all the laboratiories.The detection results were qualified in every year.The response rates for salt iodine detection were 100% except for no responses in 2001 and 2002,with an average qualification rate of 96.9% for the period.Conclusion The capability of the laboratories for IDD in Guangdong province was improved yearly during the period of 2001-2011.But the staff training and internal qulaity control need to be reinforced.
2012, 28(12): 1670-1672. doi: 10.11847/zgggws-2012-28-12-47
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2012, 28(12): 1673-1674. doi: 10.11847/zgggws-2012-28-12-49
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2012, 28(12): 1675-1675. doi: 10.11847/zgggws-2012-28-12-50
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2012, 28(12): 1676-1676. doi: 10.11847/zgggws-2012-28-12-51
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论文
Proliferation and apoptosis of SMMC-7721 induced by cadmium and its mechanism
WANG Yu, FAN Mei, GAO Ying-wei
2012, 28(12): 1599-1601. doi: 10.11847/zgggws-2012-28-12-17
Abstract(1143) HTML (179) PDF 518KB(46)
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Objective To study the effection on proliferation and apoptosis of SMMC-7721 cells induced by cadmium and its mechanism.Methods SMMC-7721 cells were incubated with 0,40,80,160μmol/L CdCl2 for 0-48 hours.Cell viability was measured by methyl thiazolyl tetrazolium assay(MTT).The occurrence of apoptosis was determined by flow cytometry.Absorption spectrometry was adopted to measure the relative activity of Caspase-3.The expression of Caspase-3 gene in SMMC-7721 cells after treatment with cadmium was determined by the methods of reverse transcription polymerase chain reaction(RT-PCR)and western-blot analysis.Results Cell viability decreased in dose-dependent manner with the dose increase of cadmium.The inhibition ratio of cell proliferation incubated with 40,80,160μmol/L CdCl2 was 33.94%,48.04%,and 68.54%,respectively.Compared with 0μmol/L cadmium,the inhibition ratio increased obviously(P<0.01).The apoptosis rate of SMMC-7721 cell incubated with 40,80,160μmol/L CdCl2 was 25.77±4.66%,34.97±9.25%,and 55.14±5.67%,respectively.Compared with 0μmol/L cadmium,the apoptosis rate increased obviously in the incubated cells with 40,80,160μmol/L cadmium(P<0.01 for all).With the dose increase of cadmium,we found that the enzyme activity of Caspase-3 increased obviously.RT-PCR analysis revealed that the incubated cell with 40μmol/L cadmium for 12,24 and 48 hours,the expression of Caspase-3 mRNA was 4.1,3.7, and 3.9 times higher compared with that of the controls.Western blot analysis revealed that in the incubated cells with 40 μmol/L cadmium for 12,24,and 48 hours,the expressin of Caspase-3 was 5.8,6.0,and 7.2 times higher compared to that of the controls,respectively.Conclusion CdCl2 can obviously restrain the proliferation and induce apoptosis of SMMC-7721 cell.The results suggest that Caspase-3 could play an important role in cadmium-induced cell apoptosis.
Effects of chronic stress on spatial learning-memory function in mice and its synaptic mechanism
LI Ya, CHEN Ya-jing, SHI Jian-xun
2012, 28(12): 1602-1604. doi: 10.11847/zgggws-2012-28-12-18
Abstract(1107) HTML (218) PDF 322KB(58)
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Objective To study the effect of chronic stress on spatial learning-memory and the role of membrane fluidity and free calcium concentrations([Ca2+]i)of prefrontal cortical and hippocampal synaptosomes in mice of different age.Methods The chronic stress model of mice was induced by stimulation of multiple stressors for 21 days.The spatial learning-memory ability of mice were tested using Morris water maze task.The changes of synaptosomal membrane fluidity and[Ca2+]i of prefrontal cortex(PFC)and hippocampus(HP)in mice brain were also examined.Results The escape latency and the swimming time of first quadrants in young and aged control mice were 63.62±4.38 and 38.63±3.04,and 76.46±3.32 and 36.24±2.65.Compared with the young control mice,the ability of spatial learning and memory were significantly decreased(P<0.05,P<0.01)in aged control mice.The anisotropy(r value)of membrane in PFC and HP were 0.1917±0.0015 and 0.1946±0.0012 in aged control mice,and significantly higher than those of the young control mice(P<0.01,P<0.05).The[Ca2+]i in PFC and HP were 1.263±0.050 and 1.465±0.063,and obvious increased compared with those of the young control mice(P<0.05,P<0.05).After chronic stress,the escape latency and the swimming time of first quadrants in young stress mice were 64.32±4.56 and 34.56±2.83.Compared with the control mice,the ability of spatial learning and memory of the stress mice were significantly decreased(P<0.01,P<0.05).The rvalue of membrane in PFC and HP were 0.1846±0.0008,0.1888±0.0010 in young stress mice and significantly higher than those of the control mice(P<0.001,P<0.01).The [Ca2+]i in PFC and HP were 1.368±0.135 and 1.956±0.170,with obvious increases compared to control mice(P<0.05,P<0.01). The aged stress mice had more serious changes after chronic stress.Conclusion The aging and the chronic stress cause damage of spatial learning and memory function in mice which may be related to the changes of membrane fluidity and [Ca2+]i of synaptosomes.
2012, 28(12): 1643-1644. doi: 10.11847/zgggws-2012-28-12-34
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2012, 28(12): 1650-1652. doi: 10.11847/zgggws-2012-28-12-37
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2012, 28(12): 1660-1661. doi: 10.11847/zgggws-2012-28-12-42
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2012, 28(12): 1661-1662. doi: 10.11847/zgggws-2012-28-12-43
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2012, 28(12): 1672-1672. doi: 10.11847/zgggws-2012-28-12-48
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