Application of liquid-phase chip technology in infectious diarrhea pathogen detection

  Objective  To evaluate application value of liquid phase chip technology in the detection of infectious diarrhea pathogens.

  Methods  From April 2015 through April 2016, 179 stool samples of diarrhea cases treated in the intestinal outpatient department of Wuxi People's Hospital (including Children′s Hospital) of Jiangsu province were collected and detected with xTAG® Gastrointestinal Pathogen Panel (xTAG GPP), and the detection results were compared to those with real-time PCR.

  Results  Totally 9 pathogens were detected in the samples from 82 diarrhea cases with xTAG GPP, with a positive detection rate of 45.81% (82/179); while, 9 pathogens were detected in 81 cases with real-time PCR, with a positive detection rate of 45.25% (81/179). The total coincidence rate for the two detections was 92.17% (165/179). The sensitivity and specificity of xTAG GPP for diarrhea pathogen detection were 84.44% and 93.33%, and positive and negative predictive value were 92.68% and 85.71%, respectively.

  Conclusion  Compared with real-time PCR detection, liquid phase chip technology is of high throughput, speed, sensitivity and specificity in common diarrhea pathogen detections, and could be used for rapid pathogenic diagnosis of diarrhea diseases.