Purification and identification of major allergens of common carp

  Objective   To isolate, idntify and purify the major allergens of common carp, which can provide academic basis for clinic.

  Methods   commoncarp proteins were isolated by SDS-poly acrylamide gel electrophoresie(SDS-PAGE)and the primary and secondary allergen proteins were identified by means of Western-blotting.The major allergens were purified by DEAE-Cellulose DE-52 Ion Exchange Chromatag raphy(IEC), and furthermore one of the primary allergen proteins was purified by Sephadex G-75 Gel Chromatography.It's bioactivity was detected by Western-blotting.

  Results   More than nine major protein bands were obtained by SDS-PAGE(50, 46, 36, 34, 26, 25, 23, 20 and 16 kDa), and the primary and secondary allergens were got by Western-blotting(42 kDa 36 kDa).

  Conclusion   The major allergens of common card are 42 kDa, 36 kDa, which are most important in establidhing a good quality and standardized fish allergen in the diagnosis and ther apy of fish allergy.