Toxicity of 95% captan in rats：an oral toxicity test

Objective To observe the toxic effects of 95% captan and to determine its possible target organ in Sprague Dawley(SD)rats.Methods Eighty SD rats were randomly divided into four groups and administered feeds containing 0(control),4 000,8 000,and 16 000 ppm of captan for 13 weeks.At the end of the study,the blood samples of the rats were collected for hematology and serum biochemistry analyses and tissues of organs were routinely prepared and stained with hematoxylin and eosin for histopathological examination.Results During the experiment,the body weight of the rats in midium and high dose group was significantly lower than that of the control group(P<0.05),and the percentage of neutrophil was increased with statistical significance compared to those of the control group;the absolute organ weights of brain,heart,spleen,liver,kindey and adrenal glands in high dose group were significantly increased compared to those of the control group(P<0.05 for all).The incidence of the edema of forestomach submucosa and inflammatory cell infiltration were significantly higher than those of the control group(P<0.05).Conclusion Based on the study,forestomach is potential toxic target organ of 95% captan.The maximum no-effect dose of captan is estimated to be 4 000 ppm.