Measurement of bone growth factor and its clinical significance in patients with humeral shortness

Objective To monitor the levels of seven bone growth factors in humeral shortness patients and normal controls.Methods Peripheral blood of 12 humeral shortness patients and 16 normal controls were collected in Nujiang basin of Tibet.The levels of bone morphogenetic protein-2(BMP-2),bone morphogenetic protein-4(BMP-4),bone morphogenetic protein-7(BMP-7),transforming growth factor-β 2(TGF-β2),fibroblast growth factor receptor-3 (FGFR3),growth differentiation factor-5 (GDF-5),and fibroblast growth factor-23(FGF23) were measured with enzyme-linked immunosorbent assay(ELISA).Results The content of BMP-2,BMP-4,BMP-7,TGF-β2,FGFR3,GDF-5,and FGF23 in humeral shortness patients were 3.35,3.14,568.24,625.09,3.01,3.26 ng/mL,and 161.33 pg/mL,respectively,and those in normal controls were 4.61,3.25,2 300.35,1849.47,5.99,8.09 ng/mL,and 373.47 pg/mL.The contents of BMP-7,FGFR3 and GDF-5 in humeral shortness patients were lower than those of normal controls,and there were significant differences between the two groups.The results of Pearson correlation analysis showed that the seven growth factors were highly correlated in the patient group,while in the normal control group,BMP-2,BMP-4,and TGF-β2 were highly correlated,but the correlations of BMP-7,GDF-5,FGFR3 and FGF23 were weak.Conclusion The causative factor of humeral shortness may be the changes of BMP-7,FGFR3 and GDF-5 and the interactions of various growth factors may affect the mcidence of the disease.