Study on DNA damage and proliferation of testicular cells with lead exposed

  Objective   To study the toxicity mechanism of mice testicular cells by lead exposure.

  Methods  

  Methods   of SCGE (Single cell gel electrophoresis technique)and MTT were used.

  Results   There were different degree DNA damage of testicular cells by lead exposed to group(0.15%), group(0.3%), group(0.6%). The length of DNA migration were significant different to the control groups(P < 0.01). Lead restrained testicular cells proliferation. MDA level were increased and SOD and GSH activity were decreased.

  Conclusion   Lead caused oxidative damage and DNA break damage of mice testicular cells, and restrained testicular cells proliferation.