Expression of fetal hemoglobin gene in maternal peripheral blood

  Objective   To explore the method for fetal hemoglobin gene expression from peripheral blood of pregnant women.

  Methods   mRNA extracted from peripheral blood of pregnant women was reversed transcript.ε and γ gene was used as a primer to amplify fetal DNA.PCR reactant were analyzed on agarose gels and observed under gel works system.

  Results   44 samples were amplified with fetal ε and γ gene and 6 with negative in 50 samples.

  Conclusion   RT-PCR methods can be used to amplify ε and γ gene directly from peripheral blood of pregnant women.Little amount of fetal mRNA from maternal blood can be nearly enough for reverse transcription and amplication.Non invasive diagnosis for fetal diseases may be possibility.