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2003 Vol. 19, No. 4

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Effects of zinc nutriture on stress-response and metallothionein in rats
WANG Dong-lan, CHENG Yi-yong, HONG Yan,
2003, 19(4): 385-386. doi: 10.11847/zgggws2003-19-04-01
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  Objective   To obser ve the changes of metallothionein and cortisol of rats in different zincnutriture under psychological condition.   Methods   Sixty rats were divided into six groups:zinc deficient(ZD),pair-fed(PF),control(CT)and the corresponding 3 stress groups(SZ,SP,SC).The psychological stress was induced by light-electric stimuli.   Results   The stressor induced more MT synthesis in brain and liver tissues while the zinc concentrations decreased to some extent in the stress animals.The zinc concentrations in sera,the MT contents in both liver and brain were decreased while the cortisol levels were increased in ZD groups.   Conclusion   It was indicated that maintaining adequate zinc nutriture was beneficial to induce the synthesis of MT in brain and liver of the rats,and to enhance their adapting ability to psychological stress.
Case-control study on risk factors of hemorrhagic fever with renal syndrome
ZHANG Yun, ZHU Jin, TAO Kai-hua,
2003, 19(4): 387-388. doi: 10.11847/zgggws2003-19-04-02
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  Objective   To investigate the risk factors and the transmission route which influences the possible hemorr hagic fever with renal syndrome(HFRS)patients.   Methods   The retrospective questionary survey is applied to identify the risk factors in the HFRS transmission by investigating 100 HFRS patients plus 200 adults fo llowing the ratio of 1:2.   Results   The main risk factors which influence the possible HFRS patients lie in skin wound,immediate contacting mouse,activity history in the plague region,mouse molestation in residence,and keeping cats ordogs by the systematic analysis usingsing lefactor and Logistic regression analysis method.   Conclusion   The above 6 items are the main risk factors in transmitting HFRS and should be avioded.
Effects of selenium on rat's hepatocellular DNA synthesis and cell cycle changes
YU Ri-an, CHEN Xue-min, LU Wen-qing,
2003, 19(4): 389-391. doi: 10.11847/zgggws2003-19-04-03
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  Objective   To explore effects of selenium on rat's hepatocellular DNA synthesis and cell cycle changes.   Methods   So dium selenite at the dose of 5, 10 and 20 μmol/kg were given to rats by i.p.and there were 5 male SD rats in each group.Hepato cellular DNA damage was measured with single cell gel electrophoresis(or comet assay), DNA relatiev content (DNARC)and cell cycle were detected with flow cytometry.   Results   At the dose of 10 and 20 μmol/kg, sodium selenite could reduce cell number of G0/G1 period, but significantly.At the dose of 5μmol/kg, sodium selenite could also reduce cell number of G0/G1 per iod, but insignificantly.The changes of proliferation index and cell number in S and G2/M period were unobvious.Sodium selenite at the dose of 5, 10μmol/kg and 20μmol/kg could increase DNA damage rate and decerease DNARC significantly.There was negative correlation between DNA damage rate and DNA relative content.Correlative coefficient was -0.998 7(P < 0.01).   Conclusion   Selenium at certain dose could change cell cycle and interfere with DNA synthesis throug h increasing DNA damage rate and decreasing DNA relative content in rat liver cells.
2003, 19(4): 391-391. doi: 10.11847/zgggws2003-19-04-04
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Association between polymorphism of angiotensin-converting enzyme gene and hypertension in mongolian people
LIU Li-juan, TONG Wei-jun, ZHANG Yong-hong,
2003, 19(4): 392-393. doi: 10.11847/zgggws2003-19-04-05
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  Objective   To explore the association between polymorphism of angiotensin-converting enzyme(ACE)gene and hypertension in Mongolian people.   Methods   On the basis of prevalence survey, fifty-one hypertensives with family history of hypertension, sixty-four hypertensives without family history of hypertension, thirty-two normotensives with family history of hypertension, eighty-five normotensives without family history of hypertension were selected as subjects in J une 2001, and polymerase chain reaction(PCR)technique was used to examine the insertion/deletion polymorphism of ACE gene.χ2 test was used to compare the differences of I/D polymorphism genetypes frenqency and I/D allele frequency among the four groups.   Results   There was no significant difference in frequency of ACE genetypes(II/D/DD)among these four groups(P > 0.1).Frequency of ID genetype is the highest, then that of II genetype and frequency of DD genetype was the lowest in the all groups.There was also no significant difference in frequency of I and D allele among these four groups.There was a similar phenomenon that frequency of I allele was higher than that of D allele in the all groups.   Conclusion   The I/D polymorphism of ACE gene was not related to hypertension in Mongolian people.
Detection of enterohemorrhagic escherichia coli O157:H7 by multiplex PCR assay
JIN Hui-ying, TAO Kai-hua, LI Yue-xi,
2003, 19(4): 394-395. doi: 10.11847/zgggws2003-19-04-06
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  Objective   To develop a method of multiplex PCR to detect E.coli O157:H7 rapidly, specifically and sensitively.To compare its sensitivity with conv entional PCR(only one sets of primers).   Methods   Four pairs of primers were designed from O antigen, H flagellar antigen and Shiga-like T oxin(SLT)1 and 2 genes.Fourty strains of O157:H7 and non O157:H7 were detected by conventional PCR and multiplex PCR amplification.And the sensitivities of PCR were estimated when the str ain was diluted from 10 1-10 6.   Results   O antigen and H flagellar antigen genes amplification generated amplicons of both 497bp and 625bp in all EHEC O157:H7 strains, SLT 1 and(or)SLT 2 genes amplification generated amplicons of 210bp and(or)484bp in toxinogenic O157:H7.Other non O157:H7 failed to yield any amplicon under comparable conditions.The sensitivity of detection by conventional PCR was shown to be at least 150CFU per PCR tube and > 1 500CFU by multiplex PCR.   Conclusion   This multiplex PCR method was more specifically, rapidly, efficient after enrichment than traditional bacterial detection.It would become a new method to diagnose the toxinogenic O157:H7 and nontoxinogenic strain.
Effects of marching with load of 1.5 kilograms in dry-heat desert on heat shock protein 70 in human lymphocyte
LIU Bing, DWU Tang-chun, WU Zhi-hao,
2003, 19(4): 396-397. doi: 10.11847/zgggws2003-19-04-07
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  Objective   To explore the regulation of heat shock protein 70(HSP70)in human lymphocyte in case of marching without load in dry-heat desert.   Methods   The lymphocyte HSP70 of 54 marchers at different speed at different time and 14 controls were detected.   Results   There was signficant difference between different speeds at the same time(P < 0.05);The lymphocyte HSP70 of group for 3 hours was obviously more than that of the control and the group for 2 hours at 3.5km/h; The level of HSP70 for 1 and 2 hours is more than the control at 5.0km/h(P < 0.05), but less than for 3 hours(P < 0.05).   Conclusion   The lymphocyte HSP70 increased with speed and time.The blood lymphocyte HSP70 was more sensitive than plasma HSP70 in the monitor of heat injure and heat tolerance.
2003, 19(4): 397-397. doi: 10.11847/zgggws2003-19-04-08
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Study on PSB's effect on regulation of lipide
XIONG Qi, ZHANG Zhao-ming
2003, 19(4): 398-400. doi: 10.11847/zgggws2003-19-04-09
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  Objective   To study the effect of photosynthetic bacteria(PSB)on regulation of lipide in serum.   Methods   The model of hyperlipidemia-rat was set up by feeding on high lipid diet.The effect of PSB on hyperlipidemia-rat was studied with the Gynostemma pentaphyllum as the positive control medicine.   Results   PSB could significantly reduce the content of TC, TG, LDL-c in serum, increase HDL-C content in serum, and increase H/L ratio.In addition, PSB could decrease the accumulation of TC in liver.   Conclusion   PSB had better curative effect than Gynostemma pentaphyllum on the regulation of lipide.
2003, 19(4): 400-400. doi: 10.11847/zgggws2003-19-04-10
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Correlation between environmental pesticides exposure and lung cancer morbidity and mortality in agricultural population
HE Qin-cheng, CHEN Jia-peng, ZHOU Bao-sen
2003, 19(4): 401-402. doi: 10.11847/zgggws2003-19-04-11
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  Objective   To make sure whether there is significant correlation between environmental pesticides exposure and lung cancer morbidity or mortality in agricultural population.   Methods   Ecological comparing method is used to collect both 1993-1998 pesticides using data and 1998-2000 lung cancer morbidity and mortality data in Shenyang subdurban area.The coefficients of spearsman correlation between pesticides exposure and lung cancer morbidity or mortality are calculated by SPSS 10.0 statistical software.   Results   The correlation between pesticides using density and morbidity of lung cancer is not significant, except 40-49 in male and 60-69 in female(P < 0.05).Mortality of 1998 in both male and all sex is significant positive correlation with pesticides use density of 1993.The coefficients of correlation are equal to 0.886(P < 0.05).Sex, age-specific analysis show that mortality of 40-49 in male, female and all sex are positive significant correlation with pesticides using density.The coefficients of correlation are 0.886, 0.886 and 0.943, respectively.   Conclusion   There is correlation between environmental pesticides exposure and lung cancer morbidity or mortality to some extent.
Effects of DDVP at different period on enzyme activity in German cockroaches
SHEN Xiao-bing, ZHANG Li
2003, 19(4): 403-405. doi: 10.11847/zgggws2003-19-04-12
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  Objective   To investigate the effects on acety lcholine esterase, car box yester ase, acid-phosphatase, 1-NA and 2-NA activity in German cockro aches exposed to DDVP at different period.   Methods   All enzyme activities were measured by visible spectrophotometry and SAS soft ware was used for statistical analysis.   Results   With the prolonging exposed to DDVP, the degree of acetylcholine esterase inhibition increase gradually at the beg inning and decrease lately.The activity of acid-phosphatase raised generally.The change of carboxy esterase, 1-NA and 2-NA activity were in opposition to acetylcholine esterase activity's.   Conclusion   A cetylcho line esterase was the main target enzyme of DDV P.Acid-phosphatase and 1-NA were the important metabolic enzymes.
Analysis on gene type of isolated strains of orientia tsutsugamushi from Heilongjiang area by way of PCR and RFLP
ZHANG Zhi-qiang, HU Ling-mei, LU Zhi-xin,
2003, 19(4): 405-406. doi: 10.11847/zgggws2003-19-04-13
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  Objective   To determined the gene type of isolated strains of orientia tsutsugamushi(Ot)in Heilongjiang area.   Methods   PCR and RFLP were used to analyse their restriction maps of sta 56 gene of the isolated strains and made a comparison between the isolated stains of Ot and the inter national reference strains.   Results   The distinctive PCR products of the 6 isolated strains from Heilongjiang areas through restriction with HinfⅠ, HhaⅠ, RsaⅠ appeared three different kinds of RFLP restriction maps.The strains MSAa9301, MSAa9303, MSAa9304, MSAp9305 had the similar restriction map with reference strain Gilliam, but they did nothave restriction spot of HinfⅠ, may be classified into the same type; strains MSAa9306 had the same restriction map with reference strain karp, may be classified into the same type; strains MSAP9302 had the same restriction map with reference strain kato, may be classified into the same type.   Conclusion   At last three types: Gilliam, Karp and Kato were exsited in the separated specise six Ot of Heilongjiang area.
Integrated analysis of effects of life style on incidence of malignant tumor
YAO Hong-yan, CHEN Hui, LV Mei-xia,
2003, 19(4): 407-408. doi: 10.11847/zgggws2003-19-04-14
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  Objective   To provide evidence for decision-making on cancer prevention through studying the trend of main life-style risk factors and their impact on cancer in Chinese people.   Methods   To estimate the population at tributable risk percent(PARP)of behavior risk factors and the effect on the cancer incidence.   Results   A ccompanying the decreasing of these risk factors such as smoking, drinking, the intake of fruit and vegetable, the incidence of the cancers(including the lung, stomach, esophageal, liver and rectum and colon cancer)will decrease to some extent.   Conclusion   It would produce significant social effect to streng then the primary prevention in the cancer prevention, which is much potential for prevention of cancer in China.
Toxicity effects of manganese on growth and cell cycle of SN4741 cells
XU Wen, CHEN Jing-yuan, WANG Feng,
2003, 19(4): 409-411. doi: 10.11847/zgggws2003-19-04-15
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  Objective   To study the effects of different doses manganese on neurocyte, SN4741.   Methods   SN4741 was incubated in culture media with 200, 400, 600, 800μmol/L manganese(MnCl2)for 2 days, 4 days and 6 days respectively.The cell growth livingness was examed by MTT and cell growth curve was made.The cell cycle was monitor ed by flow cytometry(FCM), at the same time, morphological changes was investigated by transmisssion electron microscope.   Results   Manganese of different concentrations(MnCl2 200, 400, 600, 800 μmol/L)could suppress the proliferation of SN4741 cells in dose and time-dependent manner.FCM showed that the cell cycle of the SN4741 cells could be inhibited in Speriod at certain observed through flow cytometry(FCM)and transmission electron microscope.   Conclusion   The apoptosis of SN4741 cell induced by mang anese(MnCl2)may be its important mechanism on proliferation inhibition.
Effects of fluoride on proliferation and differentiation of osteoblast in rats
ZHANG Ying, SUN Gui-fan, JIN Ya-ping,
2003, 19(4): 411-413. doi: 10.11847/zgggws2003-19-04-16
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  Objective   Effect of fluoride on proliferation and differentiation of osteoblast in rats was studied in vitro.   Methods   Various concentration of sodium fluoride(NaF)were added to the osteoblasts.The proliferative response to NaF was determined by the percents of reduced Alamar Blue.The Activity of alkaline phosphatase(ALP)was measured by Elisa method.   Results   NaF increased the proliferation of rat osteoblast at low doses(100 μmol/L-1 mmol/L), whereas athigh doses(≥2 mmol/L)it decreased the proliferation; ALP activity was increased at 10-50 μmol/L, and decreased at ≥100 μmol/L.   Conclusion   Fluoride had two-phase effects.At low doses it promoted osteoblast.s proliferation and differentiation while athigh doses ithad a negative effect.
Interaction between mutation of endothelial nitric oxide synthase gene and alcohol drinking in essential hypertension
JIA Chong-qi, ZHAO Zhong-tang, WANG Li-hua,
2003, 19(4): 414-416. doi: 10.11847/zgggws2003-19-04-17
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  Objective   To analy ze the inter action between G894T(Glu298Asp)mutation in the exon 7 of endothelial nitric oxide synthase gene and alcohol drinking in essential hyper tension.   Methods   One hundred and six teen essential hypertensives, screened from a steel factory workers, not taking hypertensive medication, and one hundred and thirty six normotensives, screened from health workers in the same factory were selected as the subjects in this study.Polymerase chain reaction (PCR)and restriction fragment length polymorphism(RFLP)were performed to detect the G894T mutation; Additive model was used to analyze the interaction; Population attributable risk percent(PAR%)was applied to calculate the etiologic fraction.   Results   There was a positive interaction between G894T mutation and alcohol drinking in essential hypertension; Attributable interaction was 3.69;Attributable interaction percent was 48.68%;Pure attributable interaction percent was 561.08%.After adjusting age, sex; smoking, body mass index by logistic regression, there was also positive interaction between G894T mutation and alcohol drinking in essential hypertension; Attributable interaction was 1.13;Attributable interaction percent was 19.09%;Pure attributable interaction percent was 22.97%.Under certain conditions, PAR% was about 10%-15%.   Conclusion   s The inter action between G894T mutation in the exon 7 of endothelial nitric oxide synthase gene and alcohol drinking had a positive effect on the essential hypertension in this studied population.To control the alcohol drinking in the population who had G894T mutation and alcohol drinking simultaneously would produce markedly decrease of the essential hypertension in general population.
2003, 19(4): 416-417. doi: 10.11847/zgggws2003-19-04-18
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Construction of clone and expression vector of gene of vibrio parahaemolyticus
LI Zhi-feng, NIE Jun
2003, 19(4): 418-419. doi: 10.11847/zgggws2003-19-04-19
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  Objective   To construct clone and expression vector of tlh gene of vibrio parahaemolyticus and examine its expression in DE 3 for preparaing monoclonal antibody and dignosing reagent and studying function of tlh.   Methods   Using VP14-90 as template, the tlh gene was amplified by modified PCR with a pair of specific primer which contained the restrictive sites of Eco RV and HindⅢ, and then subcloned into plasmid pGEM-T and expression plasmid pET32a.The recombinant plasmids were identified by restiction endonuclease enzyme analysis and PCR.The recombinant protein expression was induced by IPTG.   Results   The amplified frament was about 1.3kb by showed on the agarose gel electrophoresis and had 99% homeologous with the tlh gene in Genbank.The recombinant protein was about 63 KD.   Conclusion   The PCR method amplified the tlh gene.A clone vector and a expression vector containing tlh gene was successfully constructed, and the cell DE 3 transformed with the expression vector was capable of expression tlh gene.
Study on using DASS-FAT to detect clonorchiasis sinensis
XU Feng-quan, SHI Fa-mao, ZHANG Zhi-hua,
2003, 19(4): 420-421. doi: 10.11847/zgggws2003-19-04-20
Abstract(1031) HTML (260) PDF 202KB(45)
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  Objective   To search the use value of DASS-FAT in diagnosis of infection with clonorchiasis sinensis.   Methods   By the external culture of clonorchiasis sinensis to obtain the ES antigen and join it to Sepharous-4B, using FAT to detect sera antigen of the patients with clonorchiasis sinensis.   Results   The special antibody positive rate was 100% in sera of the patients with clonorchiasis sinensis.In the helath people, the cerebral cysticercosis, and the infection with intestinal worm, the positive rate was 2.04%(28/1372), 2.27%(2/88), and1.80%(1/55).The cross-reactive was nonsignificant(χ2=0.021, P>0.1).The negative rate was 77.78%(28/36)of the antibody in sera with clonorchiasis sinensis after treatment (12 months).   Conclusion   Using DASS-FAT to detect the special antibody in sera of the patients with clonorchiasis sinensis had high specificity and sensitivity, and the cross-reactive was nonsignificant in uninfected with clonorchiasis sinensis. The method was simple, rapid and the result of the test was stable.It can be used in the diagnosis, the examination of the effect of treatment, epidemiology and the basic study in clonorchiasis sinensis.
Relationship between ADHD and lead, magnesium
WANG Gai-qing, DU Ping, WANG Hui-cong
2003, 19(4): 422-423. doi: 10.11847/zgggws2003-19-04-21
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  Objective   To find out the relations among lead, magnesium and ADHD and provide exoteric basis for further individual prevention and cure step.   Methods   Sixty ADHD cases were diagnosed by employing ADHD Behavior Screen with DSM-IV.The control group was confirmed according to 11 matched case-control study design, then lead and magnesium in childrens distal blood and hair were collected and analyzed by atom-absorbed spectr ophotometer(graphite stove and blaze method).Data was analyzed with SAS6.12 statistics softw are.   Results   In ADHD group, the contents of blood lead were hig her(P < 0.001), the contents of blood mag nesium were lower(P < 0.001), the contents of hair mag nesium were lower(P < 0.05)than control group.There was inter actions between lead and magnesium in blood; between magnesium in blood and magnesium in hair.   Conclusion   Lead exposure and magnesium deficithelped to ADHDs occurrence.The inter acting analysis of lead and magnesium in blood showed that they antagonized each other in ADHDs etiology.
Study on relationship between hyperplasia of mammary gland and anxiety, depression, marriage quality
ZHANG He-chuang, GUO Si-zhi, ZHU Lian
2003, 19(4): 424-425. doi: 10.11847/zgggws2003-19-04-22
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  Objective   To explore the correlation between hyperplasia of mammary gland, anxiety, depression, marriage quality.   Methods   To take epidemiology investigation about 804 married women teachers, which are randomly sampled in four universites or colleges, Kunming, south-west China.Based on the above, eighty-six women which have hyperplasia of mammary gland are sampled, matching control group in proportion to 1:1 and adopting questionaire about OLSON, SAS, CES-D to investigate, and then taking analysis between case and control group by the criterion of scores of every table, which is more than or equal to x+s.   Results   The general illness ratio is 27.36%.The illness ratio of the group aged between 31 and 39 is the highest(47.66%); those of the groups aged less than or equal to 30 and between 40 and 49 are 29.47% and 23153%, respectively; that of the group aged more than or equal to 50 is the lowest(7.14%).The rates of anxiety SAS > 50 and depression CES-D > 16 are 26.05% and 30.23%, respectively, which is higher than that of the control group(P≤0.05), which are 29.07% and 24.42%, respectively.The rates of marriage satifactory > 40, couple communication > 40 and sexual intercourse > 41 are 20.93%, 24.42% and 34.88%, respectively, which is higher than that of the control group(P≤0.01)which are 37.21%, 33.72% and 44.19%, respectively.   Conclusion   The negative mood and bad marriage quality and hyperplasia of mammary gland are closely related.The intervention of the common disease must start with the psychology and social factors.
Confirmation of suitable dosage of sulfure mustard(SM) in SD rats for screening preventive and therapeutic drugs
ZHAO Yan-jun, ZHANG Hai-yun,
2003, 19(4): 426-428. doi: 10.11847/zgggws2003-19-04-23
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  Objective   To confirm the suitable dosage of sulfur mustard(SM)in SD rats for screening the preventive and therapeutic drugs.   Methods   SD Rats were used as the animal model, and four groups were established normal control group and three SM groups of different dosages, which were 3, 3.5 and 4 mg/kg(body weight)respectively.Diarrhoea rate and death rate, changes of body weight, general examination and clinical biochemistry of blood, cytological examination of bone marrow in week were observed to evaluate the toxic effetcs induced by different dosages of SM.   Results   General status: There were no animals died in all groups, but the higher the dosage of SM, the higher the diarrhoea rate was, and the percentages were 0%, 20% and 75% respectively.Changes of animals body weight showed inverse proportion to the dosage of SM.General examination of blood: The higher the dosage of SM, the more significantly the difference contrasted to the normal control group, and especially the descent of leukocytes and lymphocytes were the most signficantly.Clinical biochemistry of blood: There was no significant difference between all groups.Cytological examination of bone marrow: All groups showed that proliferation of the marrow cells were active or very active, but the cytological classification were still obviously different.The lymphocytic ratio showed significantly decreasing tendency as the increasing of dosage of SM.However, the erythrocytic ratio showed increasing tendency, while the granulocytic ratio was not obviously changed.   Conclusion   The dosage of 1mg/kg(body weight) of SM was the most suitable dosage for screening the preventive and therapeutic drugs in SD rat.
Poisonous effect of 2-methel-4-chlorophenoxyacetic acid in mice
ZHAO Shu-hua, LI Jing-shun, SUI Chun-sheng
2003, 19(4): 428-429. doi: 10.11847/zgggws2003-19-04-24
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  Objective   To observe the interferring effect of 2-methyl-4-chlorophenox yacetic acid(MCPA)in mice.   Methods   Three treated groups are divided by three dosage of MCPA(20, 100, 200 mg/kg, P.O).The weight, T 4, TSH, cholesterol, the coefficient of spleen are analyzed.   Results   After 17 days, the weight of every treat group are significantly lower than the control groups in the male mice, and the weight decreases with the increasing of the doses in the female mice.Among high-or(and)middle-dose group, TSH in blood and coefficient of spleen are significantly lower, but the content of cholesterol in blood increases compared with other treated gropus and control group.The content of T 4 in blood is abnormal significantly among low, high-dose groups.   Conclusion   MCPA can result in obstruction of mice growing, interruption of endocrine, the wither of spleen.The changes of every indexin female is more sensitive than male.
Study on universal microarray system for detection of common clinical infectious bacteria
ZHAI Jun-hui, SONG Ya-jun, DU Zong-min,
2003, 19(4): 430-431. doi: 10.11847/zgggws2003-19-04-25
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  Objective   For the rapid and efficient detection of biological agents and clinical infectious bacteria, a based detection system which employed 16SrDNA sequence as its detection target was etablished.   Methods   Oligonucleotide probes of 16 strains of bacteria were designed by using some bioinformatics softwares and methods and tested by using this microarray system.   Results   A microarray based detection system was successfully developed.The results showed that most of the bacteria can be determined at the species level.The detection limit for oligonucleotide microarray system was 200 CFU for Pseudomonas aerugi nosa and 235 CFU for Staphylococcus aureus.Aliquots of bacterial suspension were spiked into horse blood, and subject to analyse with microarray system, the sensitivity was greatly decreased to 2 000 CFU of Pseudomonas aeruginosa and 23 500 CFU of Staphylococcus aureus.But with the serial dilution of L egionella pneumoniae chromosomal DNA, a 10 times sensitivity than that of PCR-electrophoresis was observed.As for the bland test for pooled samples, two strains of bacteria in a single sample could be deteced by this method at the same time.   Conclusion   The oligonucleotide microarray could determine most of the test strains at species level.The overall time for sample process, hybridization and data acquisition lasted about 4.5 hours.
Study on toxicity and mutagenic effects of quaternary ammonium compound detergent
LIANG Xiao-yun, LI Shao-qun, DU Yan-yan,
2003, 19(4): 432-434. doi: 10.11847/zgggws2003-19-04-26
Abstract(1168) HTML (292) PDF 264KB(38)
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  Objective   To study general to xicity, hereditary toxicity and mutagenicity of quaternary ammonium compound detergent.   Methods   According to the People's Republic of China standards Procedures and methods for toxicological assessment on food safety.   Results   The LD 50 of quaternary ammonium compound detergent was 4 300 mg/kg·bw.And there had no significant difference between the experimental groups and the control group in accumulative toxicity test, Ames test, micronucleus test and chromosome aber ration test of bone marrow cell etc.In case of sperm shape abnormality test, the rate of abnormal sperm seemed to betwice that of the control group.In 30 days feeding test, there had no dramatically change between the experimental groups and the control group when the indices were the body weight, blood regular test, CH, Bun and Gluin serum etc.But the ACT in serum were higher than that of the control group(P < 0.05).The histopathology test showed that quaternary ammonium compound detergent was harmful to the liver and testis tissues.   Conclusion   Quaternary ammonium compound detergent was a kind of lower toxic substance.It was toxic to liver and testis and had hereditary toxicity to mice, which should be noticeable.
Study on mechanism of apoptosis of gastric cancer cells induced by soybean isoflavone
MA Ji-xiang, Su Jun-ying, LI Hui-qing,
2003, 19(4): 434-436. doi: 10.11847/zgggws2003-19-04-27
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  Objective   To investigate the apoptosis in gastric cancer cells induced by soybean isoflavone, and the relation between this apoptosis and expression of bcl-2 and bax.   Methods   In vitro experiments, MTT assay was used to determine the cell growth inhibitory rate.Transmission electron microscope and TUNEL staining method were used to quantitatively and qualitively detect the apoptosis status of gastric cancer cell line SGC-7901 before and after the soybean isoflavone treatment.Immunohistochemical staining and RT-PCR were used to detect the expression of apoptosis-regulated gene bcl-2 and bax.   Results   Soybean isoflavone inhibited the growth of gastric cancer cell line SGC-7901 in a dose-and time-dependent manner.Soybean isoflavone induced SGC-7901 cells to undergo apoptosis with typically apoptotic characteristics, including morphological changes of chromatin condensation, chromatin crescent formation, nucleus fragmentation and apoptotic body formation by transmission electron microscope and staining positive cells by TUNEL assay.Soybean isoflavone can reduce the expression of apoptosis-regulated gene bcl-2, and improve the expression of apoptosis-regulated gene bax.   Conclusion   Soybean isoflavone was able to induce the apoptosis in gastric cancer.This apoptosis may be mediated by down-expression of apoptosis-regulated gene bcl-2 and up-expression of apoptosis-regulated gene bax.
Test and effect evaluation on hospital sewage treated by electromagnetic fields
WANG Pei, LIN Da-rong
2003, 19(4): 437-438. doi: 10.11847/zgggws2003-19-04-28
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  Objective   To study whether sewage treated with electromagnetic fields can be reused or not.   Methods   Various water quality indicators of sewage before and after treatment were detected.Indicators of treated water were compared with those of the Domestic non-drinking Water Quality Standard.   Results   All indicaters of treated water except COD met the requiremnet of the Domestic Non-drinking Water Quality Standard.   Conclusion   Sewage treated beforehand and clarified with compoud eletromagnetic fields, can get the demand that can be reused.
2003, 19(4): 438-438. doi: 10.11847/zgggws2003-19-04-29
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2003, 19(4): 439-440. doi: 10.11847/zgggws2003-19-04-30
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2003, 19(4): 440-440. doi: 10.11847/zgggws2003-19-04-31
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2003, 19(4): 441-442. doi: 10.11847/zgggws2003-19-04-32
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2003, 19(4): 443-444. doi: 10.11847/zgggws2003-19-04-33
Abstract(737) HTML (192) PDF 187KB(24)
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2003, 19(4): 444-444. doi: 10.11847/zgggws2003-19-04-34
Abstract(698) HTML (192) PDF 248KB(28)
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Analysis on cardiovascular health status of middle-old age and its risk factors in Nanning city
XU Yong-fang, QIN Yu-qiao, CHEN Na-ying,
2003, 19(4): 445-446. doi: 10.11847/zgggws2003-19-04-35
Abstract(1215) HTML (247) PDF 198KB(40)
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  Objective   To analyse cardiovascular health status and risk factors of middle-old aged population in Nanning city.   Methods   Residents aged 35-74 in Nanning city were randomized to be the subject of a survey of cardiovascular health and to study the epidemiological tendency and risk factors of cardiovascular health.   Results   (1) The hy pertention prevalence rate arrived at 22.06% and went up with age.Overweight prevalence rate was 27.45%, of which the male was 31.28% and female was 23.66%.The prevalence for male was higher than that of female(P < 0.05). (2)The myocardia infarction, apoplexy, heart failure prevalence rates were 0.98%, 2.20%, 1.71% respectively. (3)The hyper glycemia, hyper c-holesterolemia, hyper triglyceride prevalence rates were 17.35%, 56.67%, 30.94%. (4)Using multile regression, overweight, alcohol usage, cigarette smoking, hypertension, age, education, chronic disease families were significantly associated with cardivascular disease.   Conclusion   The level of cardiov ascular disease incidence and risk factors were high in residents of Nanning.Countermeasure should be strengened further.
Epidemiological study on PTSD among 7-15 years old children in flood district
LIU Ai-zhong, TAN Hong-zhuan, ZHOU Jia,
2003, 19(4): 447-449. doi: 10.11847/zgggws2003-19-04-36
Abstract(1230) HTML (215) PDF 237KB(137)
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  Objective   To explore the incidence of PTSD and its risk factors among victims of flood, 7-15 years old children in flood district.   Methods   Diag nostic and statistical manual of mental disor ders(Ⅳ Edition)is used to examine and diagnose the victims.   Results   The positive rate of PTSD was 17.7%.The results of logistic regression analysis showed that age, style of flood, experience of flood and behavior problem are risk factors.   Conclusion   PTSD is a common mental disor der among 7-15 years old children after flood in our country.Preventive measures should be taken to protect population from PTSD in flood district.
2003, 19(4): 450-451. doi: 10.11847/zgggws2003-19-04-37
Abstract(689) HTML (170) PDF 174KB(26)
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Survey on people's affordability and willingness for malaria control cost in two ethnic minority villages
XU Jian-wei, SUN Xiao-dong
2003, 19(4): 452-454. doi: 10.11847/zgggws2003-19-04-38
Abstract(814) HTML (244) PDF 263KB(51)
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  Objective   To explore Aini and Jiruo people's affordability and willingness for malaria control cost, and study methodology in the area.   Methods   Household heads were interviewed with a questionnaire and the data were analyzed by statistics.   Results   Aini people's mean annual household and cashincome per capita at Xiangdong village was much more than ethnic Jiruo's at Shizui village, however, the mean annual medical cost per capita was less.This indicated that Aini people had a stronger affordability than ethnic Jiruo. 96.4% (95%CI: 87.5%-99.6%) of Xiangdong families and 84.1% (95%CI: 69.9%-93.4%)of Shizui families who were willing to pay for malaria control, and the mean annual amounts of willing ness to pay (MAAWP) were respectively RMB 13.28±2.21 and 4.35±1.71. However no significance was found in statistics among MAAWP of the upper, middle and lower income families.   Conclusion   People's willing ness to pay was affected by other factors as well as deter mined by their affordability.
Study on sero-indexes among 2690 qualified blood donors
YE Dong-qing, HAO Jia-hu, HUANG Fen,
2003, 19(4): 454-456. doi: 10.11847/zgggws2003-19-04-39
Abstract(848) HTML (246) PDF 558KB(37)
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  Objective   To understand infectious status of other common pathogens among donors and to provide scientific basis for increasing blood quality.   Methods   HAV-IgM, anti-HBc, HBeAg, anti-HBe were tested by enzyme linked immunosorbent assay(ELISA); Anti-EBV-IgA, anti-HCMV and anti-toxoplasm were tested by enzyme immunoassay (EIA)and indirect blood agglutination, respectively.   Results   In the first stage of the study, the percentage of donors that had at least a positive index among HAV, HBeAg, anti-HBe, anti-HBc, anti-HCMV, anti-EBV, anti-toxoplasm(7 indexes)was 30.7%.In the second stage of the study, the percentage of donors thathad at least a positive index among HBeAg, anti-HBe, anti-HBc, anti-HCMV, anti-EBV(5 indexes)was 43.2%.   Conclusion   Besides testing routine indexes, it was necessary that HBeAg, anti-HBc, anti-HCMV, anti-EBV and anti-toxoplasm be tested among donors, which can lower the incidene of transfusion transmitted diseases.
Analysis on accidental injuries based on hospitalized children from 1996 to 2000
ZHAO Xin, ZHANG Wei
2003, 19(4): 457-458. doi: 10.11847/zgggws2003-19-04-40
Abstract(995) HTML (206) PDF 178KB(29)
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  Objective   To investigate the risk factors of accidental injuries in childhood and to provide reference for the prevention of child accidental injuries.   Methods   Aretrospective study was made on 772 cases of accidental injruies hospitalized children in Tangdu hospital from 1996 to 2000.   Results   The rate of accidental injuries cases to all hospitalized children(0-14) years old was 8.13%. Deaths resulting from the injuries was 21, the death rate was 2.72%.It was the first cause for the hospitalized children deaths.The ratio between male and female was 5.43:1. The type of injuries in differential age groups were variant.The main injuries were burns(22.6%), falls(17.4%) and toxications(17.0%) in 1-5 years old group, traffic accidents(34.7%), cuts(24.4%) and sport injuries(22.2%) in 6-9 years old groups; traffic accidents(40.2%) and fighting injruies(18.8%) in 10-14 years old group.   Conclusion   The accidental injuries was a serious problem in children.The main injuries types were influenced by age, gender and other factors.Differential stratag ems should be developed to prevent accidental injuries for differential age group children.
2003, 19(4): 459-459. doi: 10.11847/zgggws2003-19-04-41
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2003, 19(4): 460-461. doi: 10.11847/zgggws2003-19-04-42
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2003, 19(4): 461-462. doi: 10.11847/zgggws2003-19-04-43
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2003, 19(4): 463-464. doi: 10.11847/zgggws2003-19-04-44
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2003, 19(4): 464-464. doi: 10.11847/zgggws2003-19-04-45
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Epidemic characteristic of malaria and preventive therapeatic measures in Longgang district in Shenzhen
WEN Xiang, LIU Shang-lin
2003, 19(4): 465-466. doi: 10.11847/zgggws2003-19-04-46
Abstract(926) HTML (240) PDF 175KB(70)
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  Objective   To analyze malaria epidemic characteristic in Longgang district, and to evaluate effects of preventive and therapeutic measures.   Methods   Using the retrospective study, the data on malaria epidemic situations, preventive and therapeutic measures and effects in Longgang district were collected.   Results   The malaria epidemic characteristic in Longgang district were as follows: (1)The incidence rate was influenced by moving population; (2)The outbreak of malaria centralized among the periods from April to October each year; (3)Most of the suffers were the youth and middle-age people.The incidence rate of malaria decreased from 158.43/10 4 in 1993 to 1.75/10 4 in 2001.   Conclusion   The malaria epidemic in Longgang was influenced mainly by moving population.And the current preventive and therapeutic measures were feasible and effective.
2003, 19(4): 467-467. doi: 10.11847/zgggws2003-19-04-47
Abstract(883) HTML (225) PDF 126KB(25)
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2003, 19(4): 468-469. doi: 10.11847/zgggws2003-19-04-48
Abstract(706) HTML (186) PDF 189KB(25)
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Analysis on monitoring result of IDD in Xining
ZHANG Xiu-li
2003, 19(4): 469-470. doi: 10.11847/zgggws2003-19-04-49
Abstract(1044) HTML (234) PDF 178KB(27)
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  Objective   To understand the present situation of iodine deficiency disoder(IDD)and the prevention effect of iodated salt in Xining city.   Methods   To investigate the present situation of IDD in whole city with probability proportional to size.   Results   In 1999 the children's goiter rate was over 5% in two region(the west region and east region).The no qualified rate of iodated salt in 1997, 1999 is higher than that of 1995, only the median of UI in the west region in 1997 was under 100 μg/L.   Conclusion   The prevention of IDD achieved obvious effect.But the monitor ing result of iodated salt in 1997, 1995 indicated that no qualified iodated salt on market was still the main reason that prevented the prevention effect of IDD.
2003, 19(4): 471-472. doi: 10.11847/zgggws2003-19-04-50
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2003, 19(4): 473-476. doi: 10.11847/zgggws2003-19-04-51
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2003, 19(4): 476-476. doi: 10.11847/zgggws2003-19-04-52
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2003, 19(4): 477-478. doi: 10.11847/zgggws2003-19-04-53
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2003, 19(4): 479-480. doi: 10.11847/zgggws2003-19-04-54
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2003, 19(4): 481-482. doi: 10.11847/zgggws2003-19-04-55
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Rapid biolu minescent technique to detect bacteria in meat
SHU Bai-hua, SUN Dan-ling, WANG Sheng-li,
2003, 19(4): 483-484. doi: 10.11847/zgggws2003-19-04-56
Abstract(1063) HTML (269) PDF 508KB(95)
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  Objective   To establish a method to dete mine the bacteria pollution of meat rapidly.   Methods   The microbial ATP and the somatic ATP are measured by using ATP-biolu minescence technique and removing somatic ATP technique.The microbial ATP content is directly proportional to bacteria count.   Results   The somatic ATP of raw meat must be removed, so the measurement of the micobial ATP content of raw meat takes bout 15 min.The somatic ATP of the cooked meat is released and hydrolyzed, so the detecting time of microbial content of these cooked cured foods can be reduced to 4 min.In the procedure applied detection limit is about 103 cfu/g.   Conclusion   The bacteria polluting situation of meat can be deter mined rapidly and timely by using A TP-biolu minescence technique.The technique can be widely used to foods sanitation.
2003, 19(4): 484-485. doi: 10.11847/zgggws2003-19-04-57
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2003, 19(4): 486-487. doi: 10.11847/zgggws2003-19-04-58
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2003, 19(4): 487-487. doi: 10.11847/zgggws2003-19-04-59
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2003, 19(4): 488-489. doi: 10.11847/zgggws2003-19-04-60
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2003, 19(4): 489-490. doi: 10.11847/zgggws2003-19-04-61
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2003, 19(4): 491-492. doi: 10.11847/zgggws2003-19-04-62
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2003, 19(4): 493-494. doi: 10.11847/zgggws2003-19-04-63
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2003, 19(4): 494-494. doi: 10.11847/zgggws2003-19-04-64
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2003, 19(4): 495-495. doi: 10.11847/zgggws2003-19-04-65
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Analaysis on mental health state and family background of juvenile delinquents
LI Hui-min
2003, 19(4): 496-497. doi: 10.11847/zgggws2003-19-04-66
Abstract(1417) HTML (398) PDF 453KB(72)
Abstract:
  Objective   To study the mental health state of juvenile delinquents, and its relations with their family back-ground and parental rearing pattern.   Methods   Two hundred and forty juvenile delinquents and 240 normal controls were tested with SCL-90, EMBU and family condition questionnaire.   Results   Scores of all factors' of SCL-90 were much higher in juvenile delinquents than in the controls.The criminals' family background was not quite good.They got lower scores in love care and understanding and gothigher scores in punishment, severity, partiality, refusal and negation of EMBU than the controls.Except love care and understanding, the other scales' scores of EMBU were significantly related with all factors of SCL-90.   Conclusion   The mental health level of juvenile delinquents was low.Their family background and parental rearing patterns were responsible for this.
Study on contributing factors of college students in support of society
LIANG Zhi-qun, LU Li, YU Jie,
2003, 19(4): 498-499. doi: 10.11847/zgggws2003-19-04-67
Abstract(1252) HTML (235) PDF 419KB(94)
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  Objective   To explore the contributing factors of college students in suppurt of society.   Methods   One thousand and forty college students were assessed with SSRS, STAI, SAD, TSBI, LES and TCSQ.Data were analysed in a multiple correlation/regression analysis with SPSS.   Results   Score of SSRS was 33.05±3.85.There was significant differences between SSRS and STAI, SAD, TSBI, TCSQ (P < 0.05). The result of logistic analysis indicated that social avoidance, social behavior, trait coping style and life eventhad great influence on social support.   Conclusion   It should be strengthened to develop healthy personality and social competence.
2003, 19(4): 499-500. doi: 10.11847/zgggws2003-19-04-68
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2003, 19(4): 501-502. doi: 10.11847/zgggws2003-19-04-69
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Study on mental health educatin for medical college students
ZHANG Yun-sheng
2003, 19(4): 502-503. doi: 10.11847/zgggws2003-19-04-70
Abstract(1065) HTML (224) PDF 402KB(49)
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  Objective   To study effective measures of mental health education for college students.   Methods   190 and 100 freshmen were selected for a experimental group and a control group.The experimental group was educated on mental health for one semester through classroom teaching, group counseling and individual counseling.   Results   Compared with the control group, there were insignificant differences in sorces of SCL-90 before the education.After the education, the experimental group had significantly lower scores than the control group except somatization and psychotism.   Conclusion   Mental health education can raise mental health level of college students.
2003, 19(4): 504-505. doi: 10.11847/zgggws2003-19-04-71
Abstract(827) HTML (222) PDF 172KB(57)
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2003, 19(4): 505-506. doi: 10.11847/zgggws2003-19-04-72
Abstract(708) HTML (191) PDF 185KB(34)
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Application of weight of information in comprehensive evaluation of behavior and health effect
YI Jing, ZHOU Yan-rong, Wang Rui-hua,
2003, 19(4): 507-510. doi: 10.11847/zgggws2003-19-04-73
Abstract(1009) HTML (231) PDF 303KB(38)
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  Objective   To use the weight of information in the comprehensive evaluation of behavior(live type)and health effect and to explore the value of it.   Methods   To make the weight of information(the weight of shannon)using shannon, then to calculate the RSR and the RSRw which the weight of shannon is used in the RSR, and to comparate the difference of the RSR and the RSRw.   Results   The range(STD, CV)of the RSRw is bigger than that of RSR and the degree of the RSRw is reasonable.YuZhongqu ShaPingba is in middle degree.   Conclusion   The comprehensive evaluation which uses the weight of information is scientific, objective and reasonable, the sub-consciousness of tense is a bad behavior.
Conditional logistic regression analysis and path analysis of genetic factors on cerebral infarction
SHI Bao-lin, ZHANG Jie, LIU Jin-xiang,
2003, 19(4): 510-512. doi: 10.11847/zgggws2003-19-04-74
Abstract(929) HTML (230) PDF 260KB(36)
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  Objective   To explore the relationship between genetic factors and the development of cerebral infarction.   Methods   One hundred and twelve cases who were primary patients of cerebral infarction in the neurology department of the first and the second affiliated hosital of Zhangjiakou Medical College were selected.One hundred and twelve controls who were patients of no cerebral infarction in same hospital during the same period were selected by the 1B1 matched case-control study.One-way and multivariate conditional logistic reg ression analysis and path analysis were used to affirm the risk factors of cerebral infarction.   Results   The incidence age of father and mother, the prevalence of siblings and second-degree relatives had positive relationship with cerebral infaction.The results of path analysis indicated that the residual variation was on the high side, and the ability of explanation was only 26%.   Conclusion   Genetic factor played a role to some extent in the incidence of infarction.The study of path analysis showed that the genetic factor was only one of risk factors, the ability of explanation was only 26%, ther efore, the environment factor was more important than other factors.