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2004 Vol. 20, No. 3

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2004, 20(3): 257-259.
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2004, 20(3): 259-259.
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Case-control study on environmental risk factors of lung cancer in Nanjing population
LIANG Ge-yu PU Yue-pu, YIN Li-hong,
2004, 20(3): 260-261.
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  Objective   To study the environmental!risk factors of lung cancer in Nanjing population.   Methods   A 1:1 match case-control study was carried out,which involved 63 cases of primary pulmonary squamous cell carcinoma and 89 cases of pulmonary adenocarcinoma.The environmental risk factors were analyzed by conditional logistic regression model.   Results   Chronic bronchitis and quantity of smoking were found to be risk factors to lung squamous cell carcinoma.The OR value was 8.55,2.35,respectively,and population attributable risks(PAR)for each of them was 18.22%,58.80%,respectively,the total PAR was 68.71%.The risk factors related to lung adenocarcinoma were chronic bronchitis,the history of pulmonary tuberculosis,bad cooking habituation,use of coal stove and family histoty of cancer.The respective OR value was 5.16,4.12,2.91,2.02,2.59,and PAR was 8.15%,11.91%,27.30%,29.98%,10.35%,respectively.Summary PAR for the five factors above was 63.06%.   Conclusion   The development of pulmonary squamous cell carcinoma and adenocarcinoma was related with various environmental factors respectively.Strengthening the control of the environmental risk factors was suggested to reduce the incidence of lung cancer.
Spatial analysis of snails distribution in marshland of Jiangning county
ZHANG Zhi-ying, XU De-zhong, PENG Hua,
2004, 20(3): 262-263.
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  Objective   To explore the spatial distribution of oncomelenia snails in marshland of Jiangning county in 2000.   Methods   The Spatial Scan Statistics and the Geographic Information System(GIS)were used to detect the spatial aggregation of Oncomelania hupensis in marshland of Jiangning County.And the semi-variogram was established to analyze the spatial auto-correlation of snails's distribution.   Results   The GIS for snail habitats showed that the habitats in marshland mainly distributed along the Yangtze river in 2000.And the Spatial Scan Statistics detected 2 spatial clusters for alive snails and 2 for snails infected by schistosome in marshland respectively.The further analysis demonstrated that the semivariogram for the distribution of snails in spatial were spherical model, which means that the variation of snails distribution in spatial were related with the distance apart when the distance was less than 0.030 1 and the variation can be estimated by the semi-variogram.   Conclusion   The spatial aggregations and auto-correaltion of alive snails indicated that there were some factors suitable for the survival of snails in marshland of Jiangning county.So the study on the spatial distribution of oncomelenia snails was very useful for the further research on the risk factors.
Effects of soybean isoflavone on bone biomechanics in ovariectomized rats
NA Xiao-lin, LIU Xiao-hua, CUI Hong-bin
2004, 20(3): 264-265.
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  Objective   To explore the benefit effects of soybean isoflavone on BMD and bone biomechanics property in ovariectomized rats.   Methods   The rats were sham-operationed and divided into 6 groups.There were Ovx group(ovariectomized control group)soybean isoflavone group, estrogen group and Sham group.After feeding sixteen weeks, the rats were killed and femur were separated.BMD and flexural strengh and flexural elastic module were measured.Bone wet weights and dry weights and ash weights were weighed.   Results   The BMD of Ovx group decreased significantly and femur biomechanics markers changed obviously.Soybean isoflavone and estrogen can increase BMD(P < 0.01)and express dose-dependent manner.Flexural strengh and flexural elastic module increase.Femur length and different state weights in estrogen group were significant lower than other groups and there were no obvious changes in other groups.   Conclusion   Soybean isoflavone and estrogen can increase femur BMD and had benefit effects on bone biomechanics property.
Inhibiting function of BRF on Tlymphocyte expression in apolipoprote in E deficient mice
XIA Min, ZHU Hui-lian, TANG Zhi-hong,
2004, 20(3): 266-267.
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  Objective   To explore the effect of black rice outer layer fraction(BRF)on CD 4 + T, CD 8 + T lymphocyte expression in apoE-deficient mice and find the possible mechanism of BRF in anti-atherogenesis.   Methods   CD 4 +T and CD 8 +T lymphocyte expression in aortic sinus and atherosclerotic lesion area in aortic sinus were assessed by immunohistochemistry and image analysis, respectively.   Results   BRF inhibited CD 4 +T lymphocyte expression and atherosclerotic lesion area in aortic sinus.   Conclusion   BRF dramatically reduced CD 4 +T lymphocyte expression and this effect maybe related to its antiatherogenesis.
Detection for hantavirus in chigger mite with microarray techniques
ZHU Jin, TAO Kai-hua, CAO Min,
2004, 20(3): 268-270.
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  Objective   To establish a rapid, sensitive and specific diagnostic assay for Hantavirus with microarray techniques.   Methods   Specific primers and probes were designed according to the conservative and specific DNA sequence of 76118 strain and R22 strain.The probes were spotted on glass slides to form microarrays.The Cy3-labled single strand DNA fragment prepared by dissymmetical PCR were hybridized with the probers on the glass slides.The microarrays were scanned and analyzed with a scanner.   Results   DNA microarray could detect the different typed DNA of HTN and SEO with adequate specificity and sensitivity.   Conclusion   The successfully developed DNA microarray and techniques might be a very useful method for diagnosis and prevention, and widely applied in specific pathogen detection of infectious diseases such as hemorrhagic fever with renal syndrome.
Effects of selenium against fluoride on DNA damage and apoptosis of human hepatocytes
XIA Tao, YANG Ke-di, ZHU Qi-long,
2004, 20(3): 270-271.
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  Objective   To investigate the influence of selenium and fluoride on DNA damage and apoptosis in human hepatocytes in vitro.   Methods   The DNA damage rate, cellular apoptosis, and cell cycle in human hepatocytes were measured after hepatocytes were incubated with fluoride and/or selenium for 12 hours in vitro.   Results   The DNA damage rate, apoptosis, and cell number in Sphase in hepatocytes in the fluoride group were significantly higher than the control group and the fluoride-exposed and selenium-treated groups(P < 0.05).The DNA damage rate and apoptosis in hepatocytes in the selenium group were higher than control group, but there was no significant difference between selenium group and control group(P > 0.05).   Conclusion   Some dose of selenium can antagonize DNA damage and apoptosis in hepatocytes induced by fluoride.
Inhibitory effect of grape procyanidin on abnormal expression of parp mRNA in rat's hepatic cells
CONG Hong-qun, ZHONG Jin-yi
2004, 20(3): 272-273.
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  Objective   To study the effect of grape procyanidin(GPC)on N-nitroso compounds-induced expression of poly(ADP-ribose)polymerase(PARP)mRNA in rat's hepatic cells.   Methods   0.5% NaNO2 was infused into Wistar rats to induce hepatocytes.mutation, and GPC was infused by 100 and 10 mg/kg respectively through mouth of the two experimental groups at the same time.Aftereight weeks, hepatic tissue was fixed in vivo and situ hybridization was applied to measure the expression level of PARP mRNA in rats.hepatic cells, and its expression level was quantitatively analyzed by an image analysis instrument.   Results   The positive cells rate of PARP mRNA expressed in the induced-injury group was 30.12% and optical density was 0.354 3±0.041 9, and those of the high-dose GPC group were 11.48% and 0.268 7± 0.017 2, and the difference between the two groups had statistical significance(P < 0.01).   Conclusion   GPC had inhibitory effect on N-nitroso compounds-induced abnormal expression of PARP mRNA in hepatic cells of rats.
Amplification and cloning of staphylococcus aureus enterotox in D(SED) gene
LONG Jun, CHEN Qing, YU Shou-yi
2004, 20(3): 274-275.
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  Objective   To construct a recombinant plasmid containing staphylococcus aureus toxic protein gene.   Methods   A couple of primers were designed for PCR according to the known sequence of SED.The gene obtained by amplification from plasmid DNA of staphylococcus aureus by PCR technique was cloned into plamid of p ET32a directionally.The recombinant plasmid p ET32a was transferred into competent J M109.The recombinants were screened and identified by restriction analysis and PCR, the cloned gene was sequened.   Results   The size of amplified PCR products was 317bp.The correct recombinant plasmid p ET32a was isolated and confirmed by restriction analysis and PCR, DNA sequencing showed the DNA sequence of the cloned gene was the same as the published sequence.  Conclusions   The SED toxic gene was first successfully amplified and cloned into plasmid PET32a in our country.It provided the basic material for studying the pathogenesis.
2004, 20(3): 275-276.
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Effects of environmental nonferrous metal pollution on health of population
NIU Jing-ping, DING Guo-wu, LIU Xing-rong,
2004, 20(3): 277-278.
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  Objective   To explore the effects of environmental pollution of nonferrous metal to health of population.   Methods   The 1 104 people in area polluted with nonferrous metal and 632 people in control area were selected with complete group sampling and their health condition were surveyed.   Results   (1) The prevalence rate was significant different between polluted and control area(P < 0.01);(2)The prevalence rates of the disease of respiratory system, nervous system, skin, arthritis, toothache, bone hyperplasia, protrusion of intervertebral disc and damage of kidney were significant different between polluted and control areas(P < 0.01);(3)The prevalence rates of cold, trachitis, asthma were significant different between polluted and control areAS(P < 0.01), the prevalence rates of pharyngitis, hypersensitivity rhinitis were marked different between polluted and control areas(P < 0.05);(4)Theprevalence rate in people aged over 50 years was significant different between polluted and control areas(P < 0.01).   Conclusion   Environment pollution of nonfemousmetal can influence the people's health.
Study on correlativity between infection of ureaplasma urealyticum and seminal plasma inhibition material, spermatic quality
WANG Yong, HAN Xiao-dong, HOU Ya-yi,
2004, 20(3): 279-280.
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  Objective   Correlativity between Seminal plasma immunosuppressive factor(SPIF), pH value, liquefacient duration and infection of Ureaplasma Urealytlcum(UU)from normal fertile and infertile men was studied.   Methods   To select infertile men to detect regular indexes in seminal fluid and SPIE, pH value, liquefacient duration.To detect infection rate of unpreaplasma and urealyticum in group with normal detected values and group with at least one abnormal index, respectively.   Results   The contents of SPIF were super high or low, pH value was abnormal and liquefacient duration was very long in the four infertile groups.The positive rates of UU infection within the four infertile groups were significantly higher than that of normal fertile men.   Conclusion   Infection of UU may induce changes of the content of SPTF, pH value and liquefacient duration.
Effect of chronic alcohol consumption on rat spermatogenesis and sexual hormones
XIE Li-jun, ZHAO Song, HU Wen-yuan
2004, 20(3): 280-281.
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  Objective   To investigate the effect of alcohol consumption on the testis spermatogenesis and serum sexual hormones testosterone(T) luteinizing hormones(LH)follicle stimulating hormones(FSH) of male rats.   Methods   40 healthy Sprague-Dawley adult male rats were randomly divided into four groups.Different doses of alcohol(0, 2.7, 4.5, 7.5 g/kg) were adminstrated to the rats for 13 weeks by a gastric tube.The sperm counting, motility and the percent of abnormal sperm were observed.Serum sexual hormones(T, LH, FSH)were determined.The pathol gical changes of testicle tissue of rats were simultaneously observed by microscopy.   Results   Compared with control group, the sperm counting and motility of the three alcohol groups were decreased(P < 0.05).The frequency of abnormal sperm was increased(P < 0.05).Light microscopic evaluation of oestes revealed germ cells degeneration in tubular lumina of alcohol-treated rats.The degree of testicular injury was associated with the dose of alcohol.Serum T level of each alcohol-treated group was significantly lower than that of the control group(P < 0.01).Serum L H and FSH levels of alcohol-treated rats were also lower than those of controls(P < 0.05).   Conclusion   Alcohol is a known testicular toxin and its chronic use leads to spermatogenesis and steroidogenesis inhibition as well as hypothalamic-pituitary axis dysfunction in gonadotropin release.
Evaluation on reliability and validity of SF-36 scale in pulmonary tuberculosis patients
HE Chao-yang, ZHANG Bo-ran, LI Mei-hua
2004, 20(3): 282-283.
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  Objective   To evaluate the reliability and validity of SF-36 scale to pulmonary tuberculosis patients and to explore feasibility in TB patients.   Methods   SF-36 scale was applied to survey quality of life of TB patients under cluster sample.   Results   Result of factor analysis constant with hypotheses in structure validity analysis.Cronbach coefficient of all domains are greater than 0.7 except General Health domain.Therefore, reliability was good for TB patients.   Conclusion   SF-36 sacle was useful for measurement and assessment of TB patients qulity of life.
Effect of tea polyphenols on subunit expressions of endothelial cells
YING Chen-jiang, Zhou Yi-kai, ZHANG Xi-ping,
2004, 20(3): 284-285.
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  Objective   To search for the mechanism of tea polyphenols on cardiovascular diseases, the expressions of NADPH oxidase subuints in endothelial cells were observed.   Methods   Tea polyphenols were co-incubated with bovine carotid artery endothelial cells(BCA ECs)in vitro and the intracellular NADPH oxidase subunits p22phox and p67phox proteins were detected using western blotting method.   Results   Both 0.4 and 4.0μg/ml tea polyphenols(either green tea or black tea)down-regulated NADPH oxidase p22phox and p67phox expressions in a dose-negative relationship(P < 0.05).   Conclusion   Tea polyphenols can regulate the enzyme that played an important role in the process of ROS production in endothelial cells, which may be benefical for the prevention of endothelial cell dysfunction and the development of cardiovascular diseases.
Use of multiple imputation to assess infection rate of schistosomisasis japonicum
ZHOU Yi-biao, ZHAO Gen-ming, JIANG Qing-wu
2004, 20(3): 286-288.
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  Objective   To assess the schistosomiasis infection rate of the surveillance spots.   Methods   One surveillance spot was sampled at random from the twelve national surveillance spots, in which resident were screened by blood detection first and then were diagnosed by stool examination.The factors related with schistosomiasis infection were survey be questionnaire, and the infection rate of schistosomiasis was assessed by multiple imputation.   Results   In Quanxing village, the proportion of stool-positive resident assessed by the multiple imputation was about 20% and the proportion of resident who would show positive in the underwent stool examination was 8 percent among the 415 resident who were negative by IHA detection.   Conclusion   The proportion of stool-positive resident was underestimated in Quanxing village, and the underestimated proportion was about 5 percent.
2004, 20(3): 288-288.
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Correlation between pesticides exposure and morbidity and mortality of breast cancer
CHEN Jia-peng, LIN Gang, ZHOU Bao-sen
2004, 20(3): 289-290.
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  Objective   To make sure whether there is significant correlation between environmental pesticides exposure and breast cancer morbidity or mortality in agricultural women.   Methods   To collect both using data of pesticides in suburban areas from 1990 to 2000 and breast cancer morbidity and mortality data of Shenyang in 2000.To regard pesticides using density of 11-year aver age as independent variable, age-group breast cancer morbidity and mortality as dependent varible, respectively.The coefficients of rank corrleation and linear regression between pesticides exposure and breast cancer morbidity and mortality are carried out by spss 10.0 statistical software.   Results   The positive significant correlation is observed between pesticides using density and breast cancer morbidity and mortality in people of 35-64 age group, respectively.The coefficients of correlation and P value are 0.829(0.042), 0.943(0.005), respectively.Further analysis finds that 50-59 age group morbidity and 40-49 age mortality has significant positive correlation with pesticides using density.The coefficient of correlation and value are 0.829(0.042), 0.886(0.019), respectively.Simple regression analysis finds that significant doseresponse relation exists between 35-64 age mortality and pesticides using density.The regression coefficient is 4.448(P < 0.05).   Conclusion   Environmental pesticides exposure is relative to morbidity and mortality of breast cancer in agricultural women.Linear relation is significant between 35-64 age mortality and pesticides using density.
Sequence analysis on hemagglutinin gene of mealses virus isolated in Shenzhen
ZHUO Fei, CHEN Wei-hong, LIU Wei-min,
2004, 20(3): 291-292.
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  Objective   To explore the genetic characterization and genotype of measles viruses circulating in Shenzhen city during the year of 2000-2002.   Methods   Extracting RNA from throat swabs and urine of the measles patients, using RT-PCR amplification measles virus hemagglutinin gene of 571bp.PCR products were cloned into PM D18-T vector and the homogeneity of the virus isolated were compared with the strains in genebank.   Results   H genes homogeneity was between the range of 96.2%-100% compared with other wild-type viruses of H1 genoty pe, the homogeneity was 96.4%-99.3%.   Conclusion   Shenzhen circulating measles viruses were belong ed to the clade H1.
2004, 20(3): 292-292.
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Prediction and evaluation of population at high risk of 2 type diabetes mellitus
ZHOU Ling, HU Xu, XU Tian-liang,
2004, 20(3): 293-294.
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  Objective   Based on a nested case control study on the risk factors of type 2 diabetes and IFG, mathematical models for the risk factors of the disease were developed.   Methods   Waist hip ratio or waistline was used for the predicting index of population at high risk of 2 type diabetes, and the effectiveness was evaluated.   Results   For under the age of 40 years, the sensit ivities were 100%.The specificities were 80% and 77% and Youden's index were 0.80 and 0.77, respectively.The effectiveness of over 40 years was lower than under the age of 40.   Conclusion   WHR or waistline was a simple and effective predicting index for under 40 years of age.
Effects of vitamin on nitric oxide synthase activity of alveolar macrophage
LIU Yi-min, ZHANG Jing, ZHU Hua-feng,
2004, 20(3): 295-296.
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  Objective   In order to study the effects of vitamin E and vitamin C on the injury of alveolar macrophages(AM) exposed to quartz in vitro.   Methods   Different dosages of vitamin E and vitamin C were respectively added into AM medium, cultured with exposure of quartz in vitro.Cell activities of the three groups were observed.Also nitric oxide(NO) and the activity of nitric oxide synthase(NOS) in AM were carried out.   Results   Vitamin E and vitamin C could increase survival rate and the activity of AM.At the same time, activities of NO and NOS were decreased, especially the combination of VE and VC both at 100 μmol/L consistency, having the dramatic inhibitory effect.   Conclusion   VE and VC can inhibit the activity of NOS, decrease the amount of NO, antagonize the injury of AM exposed to quartz.
Analysis on change in iodine concentration in salt at households in Jiangsu province
WANG Pei-hua, ZHAO Jin-kou, ZHANG Qing-lan,
2004, 20(3): 296-297.
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  Objective   To examine the change in salt iodine concentration at households and to afford basic statistics for government decision-making.   Methods   To analyze data on iodine concentration collected in biennial survey of iodine deficiency disorders(IDD)from 1997 to 2002 in Jiangsu province.   Results   The coverage rate and adequacy rate of household iodized salt rose from 90.8%、65.5% in 1997 to 97.9%、96.3% in 2002, respectively.Mean concentration of iodine in salt dropped from(48.51±24.24)mg/kg in 1997 to(32.65±5.94)mg/kg in 2002.The number of areas where coverage rate and adequacy rate of iodized salt were all above 90% increased over the period of 1997 to 2002.   Conclusion   Implementation of universal salt iodization was potentially sustainable.It was advised that amount of iodine added to salt in mild IDD areas could be reduced to be(30±5)mg/kg while still meeting the requirements.
Investigation on factors affecting purification of HAV with chloroform
XIE Zhong-ping, SONG Xia, LI Weng-zhong,
2004, 20(3): 298-299.
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  Objective   To investigate the factors relating to the purification of HAV.   Methods   It was performed by the orthogonal experiment method to analyze some factors relating to the purification of HAV with chloroform.   Results   The matches between the powers of sonication and the sample volumes of virus were important factors in the titration of virus.It was better to use PBS and NS as washing buffer than MEM.The quality of chloroform also influenced the purification of HAV.   Conclusion   The purification of HAV was influenced by several factors, and it was necessary to optimize these factors to reduce the influence on the activity of HAV.
2004, 20(3): 299-299.
Abstract(620) HTML (237) PDF 118KB(33)
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Analysis of temporal trend and effect of age on primary liver cancer incidence
SHEN Qi-jun, ZHANG Xue-feng, CHEN Jian-guo,
2004, 20(3): 300-301.
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  Objective   To analyze temporal trends and effect of age on primary liver cancer(PLC)incidence in Qidong.   Methods   Age-period-cohort models was used to analyze data of twenty five years PLC incidence in Qidong to delineate period, birth cohort and age effect.   Results   There are some temporal period effects in Haidong and Lüsidistricts; a significant moderation or decrease trends in incidence risk begin with cohort around 1958-1962.   Conclusion   There was no descending trend of PLC incidence totally and the incidence risk of the birth cohorts after the 50's of the 20th century drop off.
Effect of community-based comprehensive prevention and control on cardio-cerebro-vascular disease in rural areas
CHEN Wen-li, CAI Cheng-huo, HUANG Ting-biao,
2004, 20(3): 302-304.
Abstract(786) HTML (222) PDF 275KB(63)
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  Objective   To evaluate the effectiveness of community-based comprehensive prevention and control on cardio-cerebro-vascular disease(CVD)in large scale population through interventions with high efficacy.   Methods   Guzhen community with 63 thousands of population were selected for interventions, which included hypertension management, balance nutrition, smoking control and diabetes management.   Results   After 10-year interventions, the rates of awareness, management and control of hypertension were 70.29%, 80.50% and 45.80% respectively.The levels of blood pressure among intervention site were reduced by 7.23 mmHg for systolic blood pressure and 3.92 mmHg for diastolic blood pressure.The levels of CVD related knowledge, attitude and behavior(KAB)were increased clearly.The incidence and mortality of stroke were downtrend year after year, and reduced 41.07/100 000 and 38.73/100 000 respectively in six years.While the incidentage was postponed.The economic analyses made known that the ratio of benefit-cost was 2.32 mean.   Conclusion   Community-based comprehensive prevention and control on CVD was effective, but the power and extent of interventions must be enhanced.
2004, 20(3): 304-304.
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Edible safety and nutritional composition analysis of L.wheeleri
ZHANG Feng-lei, DAI Gui-xun, ZHANG Chun-he,
2004, 20(3): 305-306.
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  Objective   To investigate the toxicity and edible safety of L.wheeleri, and analyze the nutritional composition, try to acquire scientific evaluation for L.wheeteri.   Methods   The toxicity in different tissues of samples was tested by mouse bioassay system.The Knowledge, attitude and behavior on pufferfish in denizens was investigated.The nutritional composition of L.wheeleri was tested.   Results   The toxicity of L.wheeleri was not detected.The denizens think it as a toxin-free pufferfish species to be consumed.It contained rich essential amino acids and useful mineral elements such as Mg, Zn.The contents of EPA & DHA in liver were rich.   Conclusion   L.wheeleri in Dapeng Bay had rich nutrient but toxin-free, can be considered as a safely consumble pufferfish species.
2004, 20(3): 306-306.
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Distribution of beta 3-adrenergic receptor Trp64Arg mutation in school-aged children
MO Bao-qing, LI Zhong, ZHAO Yan
2004, 20(3): 307-308.
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  Objective   To learn the distribution feature of beta 3-adrenergic receptor β(3-AR)Trp64Arg mutation in school-aged children in Nanjing and its relationship with childhood obesity.   Methods   β3-AR Trp64Arg mutation was detected with the method of RFL P among 177 school children, their height and weight were measured.   Results   The mutative C allee was 0.24 for all the children, 0.226 for boys and 0.267 for girls.The frequency of C allee and the genotype Trp64Arg was higher in obese girls.The frequency of the genotype Trp64Arg was 70.3%, 71.4% for obese girls with obese parents or obese mothers.Body weight and BMI of the children with β3-AR Trp64Arg mutation were also higher.The risk for obesity in girls with β3-AR Trp64Arg mutation was 4.5.   Conclusion   There was distribution of β3-AR Trp64Arg mutation in school-aged children in Nanjing, especially in obese girls.It may be related to obesity.
Study on determination of K、Na、Mg、P、Cu、Fe、Mn、Zn in fetal cerebra by microware dissolution and inductively couple plasma-AES spectrometry
LIU Xiao-li, XIE Jian-bin, LIU Gui-hua,
2004, 20(3): 309-310.
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  Objective   To establish the method for the determination of biological essential elements K、Na、Mg、P、Cu、Fe、Mn、Zn in Fetal Cerebra.   Methods   The samples were digested by closed-vessel microwave HNO3+H2O2 dissolution system.The solution was directly analyzed by ICP-AES for the determination of K、Na、Mg、P、Cu、Fe、Mn、Zn.   Results   The detection limits of each element was between 0.007 mg/L and 0.1 mg/L.Standard linear comelationr≥0.999.Standard deviations were below 2%.Recoveries of spiked saniples were of the range 93.7% and 116.1%.Two standard reference materials nanlely "pig Liver GBW08551" and "Human Hair GBW07601" were used an quality control standards and the analysis results conformed well to the certified values.   Conclusion   The method was proved to be simple, rapid, accurate and reliable.And it has been applied to the analysis of the sample with satisfactory results.
2004, 20(3): 310-310.
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Study on DAN damage of germ cells of mice treated with plumbum(Ⅱ) or cadmium(Ⅱ)
LIU Xiao-mei, SHI Long, JIN Ming-hua,
2004, 20(3): 311-312.
Abstract(927) HTML (299) PDF 578KB(54)
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  Objective   To study the effects of plumbum(Ⅱ) or cadmium(Ⅱ) on DNA damage of germ cells of male mice in vitro.   Methods   The DNA damage was observed by means of SCGE assay.   Results   Different dose plumbum(Ⅱ) or cadmium(Ⅱ) could cause DNA damages in male mice germ cells and there was significant dose-effect relationship.   Conclusion   Plumbum(Ⅱ) or cadmium(Ⅱ) could cause DNA damage of germ cells in vitro.
Risk factors of metabolic syndrome
OUYANG Feng-xiu, JIANG Qing-wu, YU Shun-zhang,
2004, 20(3): 312-313.
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  Objective   To identify risk factors in patient with metabolic syndrome(MS).   Methods   A case-control study of 95 cases with MS and 130 control subjects were performed.Statistical analysis of univariate, logistic regression were used to identify risk factors of MS.   Results   Age, family history of diabetes and alcohol intake enter the logistic equation by statistic significance(P < 0.05).OR value of age was 1.15, OR value of family history of diabetes was 2.79, OR of alcohol intake was 4.05.   Conclusion   Age, family history of diabetes and alcohol intake were risk factors for MS.
Effect of γ-interferon on lung silicosis of rats exposed to quartz dust
XIE Ni, CHEN Ying, LIU Wan-yang,
2004, 20(3): 314-315.
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  Objective   To explore the preventive and therapeutic effect ofγ-interferon to experimental silicosis by studying the effect of γ-interferon on lung/body coefficient and hydroxyproline in rats exposed to quartz dust.   Methods   Female Wistar rats were exposed to lower dose(20 mg/rat)of quartz dust by intratracheal injection.Beginning from the second day, the experimenatal rats were treated withγ-interferon(1×105 IU/rat)daily.Rats were killed after 1 and 2 month.Changes of lung/body coefficient and lung hydroxyproline content were determined.   Results   The lung/body coefficient showed no significant difference between quartz control groups and treated groups.The lung hydroxyproline of treated groups were significantly lower than that of quartz control groups(P < 0.05).   Conclusion   γ-interferon could significanlty reduce lung hydroxyproline content of quartz exposed rats.This denoted thatγ-interferon may inhibit or delay early silicotic fibrosis.
2004, 20(3): 315-315.
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Cross-resistant adapting response induced by radiation and mitomycin C
WANG Bin, ZHANG Hai-ying, PIAO Chun-ji
2004, 20(3): 316-317.
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  Objective   To probe the laws of the cross-adapting response induced by low dose radiation and low concentration mitomycin C.   Methods   The human lymphocytes, bone marrow cells of mice were treated by low dose X-ray or low concentration mitomycin C before treated by high dose X-ray or high concentration mitomycin C.Some targets were observed, such as chromosome aberration of human lymphocytes, bone marrow cells of mice, micronuclear(MN), sister chromatied exchange(SCE).   Results   The targets of above all appeared cross-adapt response.   Conclusion   Cross-adapt response can be inducd by low dose radiation or by low concentration mitomycin C not only in vivo but also in vitro.
Analysis on organic compounds component and determination of phthalate acid esters in human body
LIU Hui-jie, SHU Wei-qun, LI Xue-kui,
2004, 20(3): 318-319.
Abstract(796) HTML (251) PDF 661KB(79)
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  Objective   To explore components of organic chemicals and polluted levels of important organic materials in human body, evaluate its potential hazard on population health.   Methods   The venous blood specimens from 50 health women were collected.Qualitative analysis organic chemicals and quantitative detective important organic materials in venous blood by gas chromatography/mass spectrometer(GC/MS)after extraction with hexane.   Results   158 kinds of organic materials were detected from all blood samples, a significantly high frequency of organic chemicals included 9-Octadecenoic acid(82%), heptadecene-(8)-carbonic acid(66%), di-n-butyl phthalate(62%), thiosulfuric acid(H2S2O3), S-(2-aminoethyl) ester(34%), 14-Bate-H-pregna(28%), et c.Di-n-butyl phthalate with highly checked out frequency and toxic effect was determined quantity.   Conclusion   Organic pollutants existed in human body and some of them were highly checked out.Di-n-butyl phthalate with highly checked out rate and highly concentration had reproductive developmental toxicity as prority control pollution in USEPA.It would bring about a potential adverse effect on population health(for example reproductive toxicity).It should cause our highly attention(special child-bearing period women).
Effects of chlorfenapyr on DNA damage of spleen cells and liver cells in mice by single cell gel electrophoresis
LI Xiao-yan, CHEN Xian-jun
2004, 20(3): 320-322.
Abstract(760) HTML (224) PDF 271KB(52)
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  Objective   To study the effects of Chlorfenapyr on DNA damage of spleen and liver cells in mice.   Methods   Chlorfenapyr was administrated to mice with 4.9, 9.8 and 19.6 mg/kg doses once by gavage, then the percentage of cells with comets and the DNA migration of spleen cells and liver cells were detected by single cell gel electrophoresis.   Results   Compared with the control group, the percentage of cells with comets and the DNA migration were increased significantly at all the three tested doses of the two kinds of tissue cells(P < 0.05-0.001);except for the DNA migration of spleen cells, the percentage of cells with comets of spleen cells and liver cells and the DNA migration of liver cells exhibited a significant linear dose-dependent increase(r values were 0.995, 0.9987 and 0.9999 respectively, P < 0.05);at the identical dosage criterion, the percentage of cells with comets of liver cells increased statistically at all the three tested doses as compared to the corresponding dose spleen cells groups(P < 0.01), so did the DNA migration of the 4.9 mg/kg dose of liver cells(P < 0.05).   Conclusion   Chlorfenapyr can induce the DNA damage of spleen cells and liver cells, and the effects on the DNA damage of liver cells is stronger than that of spleen cells.
2004, 20(3): 322-322.
Abstract(542) HTML (162) PDF 127KB(25)
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Effect of DNA preparation on antioxidant action in old rat's tissues
XU Yue-fei, REN Feng, ZHAO Bao-chang
2004, 20(3): 323-324.
Abstract(767) HTML (270) PDF 201KB(31)
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  Objective   To investigate the effect of exogenous DNA on the production of lipid peroxide(LPO)and the activities of superoxide dismutase(SOD)in aging rat's cardiac muscle, liver and kidney.   Methods   Twenty healthy spragueDawley(SD)male rats were chosen and randomly divided by abdominal cavity with 0, 3.0 mg of DNA preparation in every day for 22 days.All rats of each group were killed at the 23th days and their cardiac muscel, liver, kidney were collected and used in determining contents of LPO and activites of SOD.   Results   The contents of LPO was significantly lower in DNA group than in the control group.The activites of SOD were increased in DNA group.   Conclusion   DNA may have the effect on cleaning oxygen free radical and antioxidant action wich can delay the aging of cardiac muscle, liver and kidney.
Perinatal mortality and its determinants in less developed rural area
WU Zhuo-chun, LI Xiao-hong,
2004, 20(3): 324-325.
Abstract(747) HTML (223) PDF 486KB(35)
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  Objective   To use daily records of local family planning practice and assess the perinatal mortality and its determinants in a less developed rual area.   Methods   The data of pregnancy related daily records provided by township family planing service stations were anlaysed.   Results   The perinatal mortality rate(PNMR)and stillbirth rate(SBR)in studied area were 69 and 24 per 1 000 births, respectively, and the early neonatal mortality rate(ENMR)was 46 per 1 000 live births.The male and female ENMR were 29 and 69 kper thousand live biths, respectively.The PNMR was associated with parity and townksipo economic level.   Conclusion   The PNMR in the studied county was higher than that in rural areas reported earlier.The results by parity and infant sex reflected the impact of the local economy and existing family planning policy on perinatal health.
2004, 20(3): 326-327.
Abstract(766) HTML (237) PDF 557KB(32)
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Investigate on nuclear magnetic resonance spectrum and fast detecting method for hypoxia stress peptide
HU Zong-hai, HU Juan, GAO Jian-li
2004, 20(3): 327-328.
Abstract(711) HTML (228) PDF 727KB(37)
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  Objective   To investigate dactylogram information of the hypoxia stress peptide(HSP)with the Nuclear Magnetic Resonance(NMR)and to establish the fast method for detecting the HSP to provid the new measure for screening the crowd easy to sickness.   Methods   To detect the blood from the wistar rats treated in the hypoxic environment and the mountain sickness patients with the NMR, and detect to the HSP with the reagent dispensed by ourselves, optimization the experiment condition.   Results   Character absorption cusp was exited at the definite wave-length tn the serum from the 104 wistar rats, but only two was exited in the control group(62 cases), there was significant difference between the two groups.The cases of the chemistry migration expressed high of the mountain sickness and the control group were 40(43)and 2(43), showing significant difference between the two groips(P < 0.01).The NMRIH character chemistry migration expressed both the group of the experiment of the mountain animal and the group of clinical patients.The reaction time and wave-length were 30min and 560nm in the method of fast detecting.   Conclusion   The HSP was exited in the body fluid of the mountain sickness patients, the chapter of the HSP was different from the other substance as known; The method of fast detecting can provid the new medilum to select the soldier for the army and to screen the easy-sickned crowd.
2004, 20(3): 329-330.
Abstract(606) HTML (204) PDF 400KB(21)
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2004, 20(3): 331-332.
Abstract(681) HTML (211) PDF 1293KB(50)
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2004, 20(3): 333-333.
Abstract(995) HTML (329) PDF 593KB(32)
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Analysis on infection rates of blood transmission virus in intravenous drug users
LI Jian-rong, TIAN Kun-lun, WANG Jing,
2004, 20(3): 334-335.
Abstract(715) HTML (209) PDF 198KB(60)
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  Objective   In order to assess the infection condition of HBV, HCV, HGV, HSV and CMY in Intravenous Drug Users(IVDUs).   Methods   It is detected that anti-HBV, HCY, HGV, HSV, CMY markers by ELISA in 406 sera and 102 normal controls.While we detected HCV RNA by RT-PCR and Fluorescence Quantitative PCR methods.   Results   Anti-HGV, HSV and CMV positive is 1.97%, 2.71% and 3.45% respctively, total HBV infection rate is 36.45%, HBsAg carrier 5.91%, HBeAg positive 3.96% in IVDUs.HBV infection rate is 17.65%, HBsAg carrier 0.98%, HBeAg positive 1.96% in the controls.Others are negative.The infection rates and coinfection rates are very prominent in the injector cousers of IVDUs(49.01%).   Conclusion   It indicates that IVDUs is high risk population of blood transmission virus infection, especially HCV infection.
2004, 20(3): 335-335.
Abstract(755) HTML (200) PDF 216KB(39)
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Study on depression symptoms and associated factors in middle school students of Chengdu urban area
ZHANG Zhi-qun, GUO Lan-ting
2004, 20(3): 336-337.
Abstract(920) HTML (232) PDF 571KB(60)
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  Objective   To study the occurrence of depression symptoms and associated factors in middle school students of Chengdu urban area.   Methods   Multiple random sample method was used in the study.1 421 middle school students were sampled.Self-editted questionnare(including age, sex, family circumstance, experience in childhood, etc.)and Beck Depression Inventory(BDI)were used in the sampled students.   Results   The mean score of BDI was 10.98±7.39.The mean score of female was higher than that of male(P < 0.01).The most common depression symptoms included fatigue, guilt, depression mood, dissatisfaction, etc.The higher BDI scores were expressed in students of graduating class, parents' divorce family, remarried family, notharmony of family members, not reared by parents and violence or suicide behaviors in family members.   Conclusion   and teachers should discover and intervene the students' depression symptoms as early as possible.
Investigation of hospital violenee knowledge among the hospital workers
JING Chun-xia, WANG Sheng-yong, CHEN Zu-hui,
2004, 20(3): 338-339.
Abstract(831) HTML (287) PDF 646KB(31)
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  Objective   A cross-sectional study for the knowledge of violence in hospital among doctors and nurses was conducted in order to provided the basis for prevention in the future.   Methods   505 hospital workers were investigated with uniform questionnaire by cluster sampling.   Results   The study showed that the hospital workers knew something about the knowledge of hospital violence, but the general was low, only 36.4% subjects' answers were right.The following were dlought as main risk factors, such as the wrong induction of media, incoordination between doctor and patient, low Disposition of patients, and the malpractice.   Conclusion   In order to prevent it occurring, we should develop the right education, improving the relation between doctor and patient, rising the quality of health service.
Study on quality of life and its influential factors of urban poor people
FU Hua-peng, LIU Yang, GUO Jing-bo,
2004, 20(3): 339-340.
Abstract(788) HTML (215) PDF 586KB(34)
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  Objective   To investigate the quality of life and its influential factors of urban poor people.   Methods   The quality of life of 237 poor people and 261 normal people living in the city of shenyang is assessing by the short form 36.The test and logistic regression are used to test the impacts of main influential factor.   Results   The quality of life of poverty group and control group were signifcantly different.The influential factor of physical health of poor people were age, sex, cost of medical treatment, debts, aimsgiving and sickness; p The influential factor of mental health of poor people were cost of medical treatment, cost of education, debts and aimsgiving.When years of poverty increase, the scores of domains decrease except RE.   Conclusion   The quality of life of urban poor people is lower than normal population significantly, particularly for poor people between 35 and 50 years old.The quality of life of poor people is influenced by age, sex, cost of medical treatment, debts, aimsgiving and sickness, cost of education.
Relationship between quality of life and satisfaction of life of middle-age intellectual
XU Ya, CHEN Si-dong, LIN Guang-ping
2004, 20(3): 341-342.
Abstract(900) HTML (257) PDF 268KB(32)
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  Objective   To study the relationship between quality of life and satisfaction of life of middle-age intellectual.   Methods   The 463 middle2age intellectual aged 40-59 were measured by questionnaires and their physical examination was conducted.The data was analyzed with factor analysis and canonical correlation analysis.   Results   The relationship of quality of life and satisfaction of life of middle-age intellectual was positive correlation(r=0.674 8);Satisfaction of life was mostly showed by relation among people, family life, medical health care and body health.   Conclusion   The key to promote quality of life was to improve satifaction of life.
2004, 20(3): 342-343.
Abstract(819) HTML (274) PDF 270KB(33)
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2004, 20(3): 344-345.
Abstract(771) HTML (232) PDF 117KB(27)
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2004, 20(3): 345-345.
Abstract(684) HTML (229) PDF 195KB(37)
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2004, 20(3): 346-347.
Abstract(817) HTML (312) PDF 594KB(31)
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2004, 20(3): 347-347.
Abstract(666) HTML (239) PDF 307KB(28)
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2004, 20(3): 348-349.
Abstract(601) HTML (170) PDF 189KB(33)
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2004, 20(3): 349-349.
Abstract(594) HTML (221) PDF 178KB(38)
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2004, 20(3): 350-351.
Abstract(761) HTML (257) PDF 276KB(24)
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Study on social abilities, behavior problems of girls with early breast development
ZHU Hong, CHEN Lin-qi
2004, 20(3): 351-352.
Abstract(704) HTML (245) PDF 1190KB(32)
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  Objective   To investigate early-breast-development girls social abilities, behavior problems and the related factors.   Methods   43-early-breast development girls aged 6-11 were investigated with Achenbach Child Behavior Scale(for parents)and self-made questionnaire on childrens family environment.Another 160 girls were selected as control group, who were investigated with Achenbach only.   Results   The average scores of early breast development girls activity, sociality, and school achievements were 4.49, 4.83 and 5.46.There were no differences between the early breast development girls(23.3%)and controls(20.6%)on prevalence of behavior problem.The total score and the scores of delinquent behavior, aggressive behavior, cruel behavior of early breast development girls were significantly higher than controls(P < 0.05).The result indicated that the number of girl-friends was related with behavior problems of girls early breast development girls.   Conclusion   Early-breast-development girls behavior problems should not be ignored.The proper pubertal development education and comprehensive measures were necessary to prevent or to cure behavior problems of early breast development girls.
2004, 20(3): 353-535.
Abstract(795) HTML (489) PDF 123KB(37)
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2004, 20(3): 354-355.
Abstract(737) HTML (217) PDF 1179KB(32)
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Study on oxidative damage effects of cooking oil fume conden sation on BEAS-2B cell
ZHANG Rong-fang, CHEN Feng, ZHU Mao-xiang,
2004, 20(3): 355-356.
Abstract(718) HTML (238) PDF 413KB(38)
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  Objective   The reactive oxygen species(ROS)production and A TPase in human bronchial epithelial cell line induced by cooking oil fume condensation were studied through measuring extra celluar and intracellular ROS levels including super oxide anions(2)and hydrogen peroxides(H2O2), and ATPase contents.   Methods   Sepectrometric method and fluorometric method were used to measure the ROS reduced by COFC.And the activities of ATPase assayed by biochemical method.   Results   The ROS correlated with the decrease of contents of A TPase.   Conclusion   Membrane ATPase activity was inhibited by ROS, especially Ca2+ATPase and Mg2+ATPase.
2004, 20(3): 357-357.
Abstract(760) HTML (278) PDF 219KB(45)
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2004, 20(3): 358-358.
Abstract(589) HTML (176) PDF 356KB(26)
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2004, 20(3): 359-359.
Abstract(642) HTML (214) PDF 302KB(27)
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2004, 20(3): 360-360.
Abstract(663) HTML (206) PDF 118KB(24)
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2004, 20(3): 361-363.
Abstract(1135) HTML (637) PDF 910KB(69)
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Risk assessment and prediction of resistance to cypermethrin in housefly(Musca domestica)
WANG Zhi-gang, HAI Xiu-Ping, XU Yong-hong,
2004, 20(3): 363-364.
Abstract(807) HTML (234) PDF 217KB(33)
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  Objective   To assess resistance risk to cypermethr in and predict the rate of r esistance development in housefly(Musca domestica).   Methods   Houseflies collected from natural group were cultured in laboratory.Resistant strain to cypermethrin in housefly was obtained while LD50 was deter mined with the method of topical application.Based on the method described by Tabashnik(1992), the realized heritability(h2)to cypermethrin in housefly was estimated and the rate of resistance development was predicted.   Results   Continuously selected with cypermethr in for 10 generations at the average survival percentage of 54.5% in laboratory, the strain of housefly had a 50-fold increase in resistance to cypermethrin.The realized heritability(h2)of resistance to cyper methrin in housefly was estimated to be 0.157 7.The rate of resistance development for 10 fold in the housefly was predicted based on the estimation of the realized heritabilit y(h2), and it only needs 2.2-4.8 generations under the selected pressure of mortality ranged 50%-90%.   Conclusion   The resistance to cypermethrin in housefly in creased rapidly and the realized heritability(h2)was stable in 10 generations.
2004, 20(3): 365-366.
Abstract(814) HTML (247) PDF 624KB(61)
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2004, 20(3): 367-369.
Abstract(1003) HTML (419) PDF 828KB(110)
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Study on effect of wet on dust collection of central air conditioning
MA De-gang, ZHANG Huang, YE Tian-zhen,
2004, 20(3): 369-370.
Abstract(791) HTML (315) PDF 870KB(39)
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  Objective   During the spreading of respiratory epidemic, to change all-air central air conditioning system in order to clean dust and sterilize air, and to reduce the second pollution and cut off the way of spreading virus.   Methods   Standing on the dust removing, the wet filler is used to be the function part of dust collection and sterilization.   Results   The wet dust collection with filler can usually remove the 25-40 percent of dust with a diameter of 1 micron, and 75-98 percent of 5 micron, but the pressure loss is 30-130 pascal.   Conclusions   The wet dust collection with filler can clean, sterilize, humidify and cool the air.It can replace the first dust collection in the traditional air conditioning, and it is important to stop the virsus spreading by the system, improve the air quality and save the energy.
2004, 20(3): 371-372.
Abstract(526) HTML (152) PDF 402KB(35)
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2004, 20(3): 372-372.
Abstract(595) HTML (191) PDF 298KB(48)
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2004, 20(3): 373-373.
Abstract(483) HTML (115) PDF 589KB(31)
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2004, 20(3): 374-374.
Abstract(659) HTML (246) PDF 114KB(30)
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2004, 20(3): 375-376.
Abstract(548) HTML (180) PDF 510KB(26)
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2004, 20(3): 376-376.
Abstract(609) HTML (172) PDF 333KB(28)
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Change of age structure of population and its relationship to development of health business in Henan province
SHI Xue-zhong, DUAN Guang-cai, SHI Song-he,
2004, 20(3): 377-378.
Abstract(832) HTML (260) PDF 919KB(43)
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  Objective   To study the change of age structure of popoulation and its relationship to development of health business in Henan Province.   Methods   To analyse the date of the 3rd, 4th and 5th overall survey of population by SAS software.   Results   The average increase rate of the population was 1.75% per year during 1982 and 1990, and it was only 0.65% during 1990 and 2000.The age median of population increased 10 years compared with that in 1982.The number of the elderly people aged over 60 years shared 10.17% of all, and the trend was ascending.The cities where lived about 17% people of all had more than 50% health resources of the total.   Conclusion   The age structure of population changed to the old, and the area health planning must be made immediately.
2004, 20(3): 379-381.
Abstract(702) HTML (214) PDF 813KB(32)
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2004, 20(3): 381-381.
Abstract(690) HTML (231) PDF 114KB(32)
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2004, 20(3): 383-384.
Abstract(711) HTML (246) PDF 254KB(39)
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