2004 Vol. 20, No. 3
Display Method:
2004, 20(3): 260-261.
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Objective To study the environmental!risk factors of lung cancer in Nanjing population. Methods A 1:1 match case-control study was carried out,which involved 63 cases of primary pulmonary squamous cell carcinoma and 89 cases of pulmonary adenocarcinoma.The environmental risk factors were analyzed by conditional logistic regression model. Results Chronic bronchitis and quantity of smoking were found to be risk factors to lung squamous cell carcinoma.The OR value was 8.55,2.35,respectively,and population attributable risks(PAR)for each of them was 18.22%,58.80%,respectively,the total PAR was 68.71%.The risk factors related to lung adenocarcinoma were chronic bronchitis,the history of pulmonary tuberculosis,bad cooking habituation,use of coal stove and family histoty of cancer.The respective OR value was 5.16,4.12,2.91,2.02,2.59,and PAR was 8.15%,11.91%,27.30%,29.98%,10.35%,respectively.Summary PAR for the five factors above was 63.06%. Conclusion The development of pulmonary squamous cell carcinoma and adenocarcinoma was related with various environmental factors respectively.Strengthening the control of the environmental risk factors was suggested to reduce the incidence of lung cancer.
2004, 20(3): 262-263.
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Objective To explore the spatial distribution of oncomelenia snails in marshland of Jiangning county in 2000. Methods The Spatial Scan Statistics and the Geographic Information System(GIS)were used to detect the spatial aggregation of Oncomelania hupensis in marshland of Jiangning County.And the semi-variogram was established to analyze the spatial auto-correlation of snails's distribution. Results The GIS for snail habitats showed that the habitats in marshland mainly distributed along the Yangtze river in 2000.And the Spatial Scan Statistics detected 2 spatial clusters for alive snails and 2 for snails infected by schistosome in marshland respectively.The further analysis demonstrated that the semivariogram for the distribution of snails in spatial were spherical model, which means that the variation of snails distribution in spatial were related with the distance apart when the distance was less than 0.030 1 and the variation can be estimated by the semi-variogram. Conclusion The spatial aggregations and auto-correaltion of alive snails indicated that there were some factors suitable for the survival of snails in marshland of Jiangning county.So the study on the spatial distribution of oncomelenia snails was very useful for the further research on the risk factors.
2004, 20(3): 264-265.
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Objective To explore the benefit effects of soybean isoflavone on BMD and bone biomechanics property in ovariectomized rats. Methods The rats were sham-operationed and divided into 6 groups.There were Ovx group(ovariectomized control group)soybean isoflavone group, estrogen group and Sham group.After feeding sixteen weeks, the rats were killed and femur were separated.BMD and flexural strengh and flexural elastic module were measured.Bone wet weights and dry weights and ash weights were weighed. Results The BMD of Ovx group decreased significantly and femur biomechanics markers changed obviously.Soybean isoflavone and estrogen can increase BMD(P < 0.01)and express dose-dependent manner.Flexural strengh and flexural elastic module increase.Femur length and different state weights in estrogen group were significant lower than other groups and there were no obvious changes in other groups. Conclusion Soybean isoflavone and estrogen can increase femur BMD and had benefit effects on bone biomechanics property.
2004, 20(3): 266-267.
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Objective To explore the effect of black rice outer layer fraction(BRF)on CD 4 + T, CD 8 + T lymphocyte expression in apoE-deficient mice and find the possible mechanism of BRF in anti-atherogenesis. Methods CD 4 +T and CD 8 +T lymphocyte expression in aortic sinus and atherosclerotic lesion area in aortic sinus were assessed by immunohistochemistry and image analysis, respectively. Results BRF inhibited CD 4 +T lymphocyte expression and atherosclerotic lesion area in aortic sinus. Conclusion BRF dramatically reduced CD 4 +T lymphocyte expression and this effect maybe related to its antiatherogenesis.
2004, 20(3): 268-270.
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Objective To establish a rapid, sensitive and specific diagnostic assay for Hantavirus with microarray techniques. Methods Specific primers and probes were designed according to the conservative and specific DNA sequence of 76118 strain and R22 strain.The probes were spotted on glass slides to form microarrays.The Cy3-labled single strand DNA fragment prepared by dissymmetical PCR were hybridized with the probers on the glass slides.The microarrays were scanned and analyzed with a scanner. Results DNA microarray could detect the different typed DNA of HTN and SEO with adequate specificity and sensitivity. Conclusion The successfully developed DNA microarray and techniques might be a very useful method for diagnosis and prevention, and widely applied in specific pathogen detection of infectious diseases such as hemorrhagic fever with renal syndrome.
2004, 20(3): 270-271.
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Objective To investigate the influence of selenium and fluoride on DNA damage and apoptosis in human hepatocytes in vitro. Methods The DNA damage rate, cellular apoptosis, and cell cycle in human hepatocytes were measured after hepatocytes were incubated with fluoride and/or selenium for 12 hours in vitro. Results The DNA damage rate, apoptosis, and cell number in Sphase in hepatocytes in the fluoride group were significantly higher than the control group and the fluoride-exposed and selenium-treated groups(P < 0.05).The DNA damage rate and apoptosis in hepatocytes in the selenium group were higher than control group, but there was no significant difference between selenium group and control group(P > 0.05). Conclusion Some dose of selenium can antagonize DNA damage and apoptosis in hepatocytes induced by fluoride.
2004, 20(3): 272-273.
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Objective To study the effect of grape procyanidin(GPC)on N-nitroso compounds-induced expression of poly(ADP-ribose)polymerase(PARP)mRNA in rat's hepatic cells. Methods 0.5% NaNO2 was infused into Wistar rats to induce hepatocytes.mutation, and GPC was infused by 100 and 10 mg/kg respectively through mouth of the two experimental groups at the same time.Aftereight weeks, hepatic tissue was fixed in vivo and situ hybridization was applied to measure the expression level of PARP mRNA in rats.hepatic cells, and its expression level was quantitatively analyzed by an image analysis instrument. Results The positive cells rate of PARP mRNA expressed in the induced-injury group was 30.12% and optical density was 0.354 3±0.041 9, and those of the high-dose GPC group were 11.48% and 0.268 7± 0.017 2, and the difference between the two groups had statistical significance(P < 0.01). Conclusion GPC had inhibitory effect on N-nitroso compounds-induced abnormal expression of PARP mRNA in hepatic cells of rats.
2004, 20(3): 274-275.
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Objective To construct a recombinant plasmid containing staphylococcus aureus toxic protein gene. Methods A couple of primers were designed for PCR according to the known sequence of SED.The gene obtained by amplification from plasmid DNA of staphylococcus aureus by PCR technique was cloned into plamid of p ET32a directionally.The recombinant plasmid p ET32a was transferred into competent J M109.The recombinants were screened and identified by restriction analysis and PCR, the cloned gene was sequened. Results The size of amplified PCR products was 317bp.The correct recombinant plasmid p ET32a was isolated and confirmed by restriction analysis and PCR, DNA sequencing showed the DNA sequence of the cloned gene was the same as the published sequence. Conclusions The SED toxic gene was first successfully amplified and cloned into plasmid PET32a in our country.It provided the basic material for studying the pathogenesis.
2004, 20(3): 277-278.
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Objective To explore the effects of environmental pollution of nonferrous metal to health of population. Methods The 1 104 people in area polluted with nonferrous metal and 632 people in control area were selected with complete group sampling and their health condition were surveyed. Results (1) The prevalence rate was significant different between polluted and control area(P < 0.01);(2)The prevalence rates of the disease of respiratory system, nervous system, skin, arthritis, toothache, bone hyperplasia, protrusion of intervertebral disc and damage of kidney were significant different between polluted and control areas(P < 0.01);(3)The prevalence rates of cold, trachitis, asthma were significant different between polluted and control areAS(P < 0.01), the prevalence rates of pharyngitis, hypersensitivity rhinitis were marked different between polluted and control areas(P < 0.05);(4)Theprevalence rate in people aged over 50 years was significant different between polluted and control areas(P < 0.01). Conclusion Environment pollution of nonfemousmetal can influence the people's health.
2004, 20(3): 279-280.
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Objective Correlativity between Seminal plasma immunosuppressive factor(SPIF), pH value, liquefacient duration and infection of Ureaplasma Urealytlcum(UU)from normal fertile and infertile men was studied. Methods To select infertile men to detect regular indexes in seminal fluid and SPIE, pH value, liquefacient duration.To detect infection rate of unpreaplasma and urealyticum in group with normal detected values and group with at least one abnormal index, respectively. Results The contents of SPIF were super high or low, pH value was abnormal and liquefacient duration was very long in the four infertile groups.The positive rates of UU infection within the four infertile groups were significantly higher than that of normal fertile men. Conclusion Infection of UU may induce changes of the content of SPTF, pH value and liquefacient duration.
2004, 20(3): 280-281.
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Objective To investigate the effect of alcohol consumption on the testis spermatogenesis and serum sexual hormones testosterone(T) luteinizing hormones(LH)follicle stimulating hormones(FSH) of male rats. Methods 40 healthy Sprague-Dawley adult male rats were randomly divided into four groups.Different doses of alcohol(0, 2.7, 4.5, 7.5 g/kg) were adminstrated to the rats for 13 weeks by a gastric tube.The sperm counting, motility and the percent of abnormal sperm were observed.Serum sexual hormones(T, LH, FSH)were determined.The pathol gical changes of testicle tissue of rats were simultaneously observed by microscopy. Results Compared with control group, the sperm counting and motility of the three alcohol groups were decreased(P < 0.05).The frequency of abnormal sperm was increased(P < 0.05).Light microscopic evaluation of oestes revealed germ cells degeneration in tubular lumina of alcohol-treated rats.The degree of testicular injury was associated with the dose of alcohol.Serum T level of each alcohol-treated group was significantly lower than that of the control group(P < 0.01).Serum L H and FSH levels of alcohol-treated rats were also lower than those of controls(P < 0.05). Conclusion Alcohol is a known testicular toxin and its chronic use leads to spermatogenesis and steroidogenesis inhibition as well as hypothalamic-pituitary axis dysfunction in gonadotropin release.
2004, 20(3): 282-283.
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Objective To evaluate the reliability and validity of SF-36 scale to pulmonary tuberculosis patients and to explore feasibility in TB patients. Methods SF-36 scale was applied to survey quality of life of TB patients under cluster sample. Results Result of factor analysis constant with hypotheses in structure validity analysis.Cronbach coefficient of all domains are greater than 0.7 except General Health domain.Therefore, reliability was good for TB patients. Conclusion SF-36 sacle was useful for measurement and assessment of TB patients qulity of life.
2004, 20(3): 284-285.
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Objective To search for the mechanism of tea polyphenols on cardiovascular diseases, the expressions of NADPH oxidase subuints in endothelial cells were observed. Methods Tea polyphenols were co-incubated with bovine carotid artery endothelial cells(BCA ECs)in vitro and the intracellular NADPH oxidase subunits p22phox and p67phox proteins were detected using western blotting method. Results Both 0.4 and 4.0μg/ml tea polyphenols(either green tea or black tea)down-regulated NADPH oxidase p22phox and p67phox expressions in a dose-negative relationship(P < 0.05). Conclusion Tea polyphenols can regulate the enzyme that played an important role in the process of ROS production in endothelial cells, which may be benefical for the prevention of endothelial cell dysfunction and the development of cardiovascular diseases.
2004, 20(3): 286-288.
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Objective To assess the schistosomiasis infection rate of the surveillance spots. Methods One surveillance spot was sampled at random from the twelve national surveillance spots, in which resident were screened by blood detection first and then were diagnosed by stool examination.The factors related with schistosomiasis infection were survey be questionnaire, and the infection rate of schistosomiasis was assessed by multiple imputation. Results In Quanxing village, the proportion of stool-positive resident assessed by the multiple imputation was about 20% and the proportion of resident who would show positive in the underwent stool examination was 8 percent among the 415 resident who were negative by IHA detection. Conclusion The proportion of stool-positive resident was underestimated in Quanxing village, and the underestimated proportion was about 5 percent.
2004, 20(3): 289-290.
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Objective To make sure whether there is significant correlation between environmental pesticides exposure and breast cancer morbidity or mortality in agricultural women. Methods To collect both using data of pesticides in suburban areas from 1990 to 2000 and breast cancer morbidity and mortality data of Shenyang in 2000.To regard pesticides using density of 11-year aver age as independent variable, age-group breast cancer morbidity and mortality as dependent varible, respectively.The coefficients of rank corrleation and linear regression between pesticides exposure and breast cancer morbidity and mortality are carried out by spss 10.0 statistical software. Results The positive significant correlation is observed between pesticides using density and breast cancer morbidity and mortality in people of 35-64 age group, respectively.The coefficients of correlation and P value are 0.829(0.042), 0.943(0.005), respectively.Further analysis finds that 50-59 age group morbidity and 40-49 age mortality has significant positive correlation with pesticides using density.The coefficient of correlation and value are 0.829(0.042), 0.886(0.019), respectively.Simple regression analysis finds that significant doseresponse relation exists between 35-64 age mortality and pesticides using density.The regression coefficient is 4.448(P < 0.05). Conclusion Environmental pesticides exposure is relative to morbidity and mortality of breast cancer in agricultural women.Linear relation is significant between 35-64 age mortality and pesticides using density.
2004, 20(3): 291-292.
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Objective To explore the genetic characterization and genotype of measles viruses circulating in Shenzhen city during the year of 2000-2002. Methods Extracting RNA from throat swabs and urine of the measles patients, using RT-PCR amplification measles virus hemagglutinin gene of 571bp.PCR products were cloned into PM D18-T vector and the homogeneity of the virus isolated were compared with the strains in genebank. Results H genes homogeneity was between the range of 96.2%-100% compared with other wild-type viruses of H1 genoty pe, the homogeneity was 96.4%-99.3%. Conclusion Shenzhen circulating measles viruses were belong ed to the clade H1.
2004, 20(3): 293-294.
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Objective Based on a nested case control study on the risk factors of type 2 diabetes and IFG, mathematical models for the risk factors of the disease were developed. Methods Waist hip ratio or waistline was used for the predicting index of population at high risk of 2 type diabetes, and the effectiveness was evaluated. Results For under the age of 40 years, the sensit ivities were 100%.The specificities were 80% and 77% and Youden's index were 0.80 and 0.77, respectively.The effectiveness of over 40 years was lower than under the age of 40. Conclusion WHR or waistline was a simple and effective predicting index for under 40 years of age.
2004, 20(3): 295-296.
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Objective In order to study the effects of vitamin E and vitamin C on the injury of alveolar macrophages(AM) exposed to quartz in vitro. Methods Different dosages of vitamin E and vitamin C were respectively added into AM medium, cultured with exposure of quartz in vitro.Cell activities of the three groups were observed.Also nitric oxide(NO) and the activity of nitric oxide synthase(NOS) in AM were carried out. Results Vitamin E and vitamin C could increase survival rate and the activity of AM.At the same time, activities of NO and NOS were decreased, especially the combination of VE and VC both at 100 μmol/L consistency, having the dramatic inhibitory effect. Conclusion VE and VC can inhibit the activity of NOS, decrease the amount of NO, antagonize the injury of AM exposed to quartz.
2004, 20(3): 296-297.
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Objective To examine the change in salt iodine concentration at households and to afford basic statistics for government decision-making. Methods To analyze data on iodine concentration collected in biennial survey of iodine deficiency disorders(IDD)from 1997 to 2002 in Jiangsu province. Results The coverage rate and adequacy rate of household iodized salt rose from 90.8%、65.5% in 1997 to 97.9%、96.3% in 2002, respectively.Mean concentration of iodine in salt dropped from(48.51±24.24)mg/kg in 1997 to(32.65±5.94)mg/kg in 2002.The number of areas where coverage rate and adequacy rate of iodized salt were all above 90% increased over the period of 1997 to 2002. Conclusion Implementation of universal salt iodization was potentially sustainable.It was advised that amount of iodine added to salt in mild IDD areas could be reduced to be(30±5)mg/kg while still meeting the requirements.
2004, 20(3): 298-299.
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Objective To investigate the factors relating to the purification of HAV. Methods It was performed by the orthogonal experiment method to analyze some factors relating to the purification of HAV with chloroform. Results The matches between the powers of sonication and the sample volumes of virus were important factors in the titration of virus.It was better to use PBS and NS as washing buffer than MEM.The quality of chloroform also influenced the purification of HAV. Conclusion The purification of HAV was influenced by several factors, and it was necessary to optimize these factors to reduce the influence on the activity of HAV.
2004, 20(3): 300-301.
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Objective To analyze temporal trends and effect of age on primary liver cancer(PLC)incidence in Qidong. Methods Age-period-cohort models was used to analyze data of twenty five years PLC incidence in Qidong to delineate period, birth cohort and age effect. Results There are some temporal period effects in Haidong and Lüsidistricts; a significant moderation or decrease trends in incidence risk begin with cohort around 1958-1962. Conclusion There was no descending trend of PLC incidence totally and the incidence risk of the birth cohorts after the 50's of the 20th century drop off.
2004, 20(3): 302-304.
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Objective To evaluate the effectiveness of community-based comprehensive prevention and control on cardio-cerebro-vascular disease(CVD)in large scale population through interventions with high efficacy. Methods Guzhen community with 63 thousands of population were selected for interventions, which included hypertension management, balance nutrition, smoking control and diabetes management. Results After 10-year interventions, the rates of awareness, management and control of hypertension were 70.29%, 80.50% and 45.80% respectively.The levels of blood pressure among intervention site were reduced by 7.23 mmHg for systolic blood pressure and 3.92 mmHg for diastolic blood pressure.The levels of CVD related knowledge, attitude and behavior(KAB)were increased clearly.The incidence and mortality of stroke were downtrend year after year, and reduced 41.07/100 000 and 38.73/100 000 respectively in six years.While the incidentage was postponed.The economic analyses made known that the ratio of benefit-cost was 2.32 mean. Conclusion Community-based comprehensive prevention and control on CVD was effective, but the power and extent of interventions must be enhanced.
2004, 20(3): 305-306.
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Objective To investigate the toxicity and edible safety of L.wheeleri, and analyze the nutritional composition, try to acquire scientific evaluation for L.wheeteri. Methods The toxicity in different tissues of samples was tested by mouse bioassay system.The Knowledge, attitude and behavior on pufferfish in denizens was investigated.The nutritional composition of L.wheeleri was tested. Results The toxicity of L.wheeleri was not detected.The denizens think it as a toxin-free pufferfish species to be consumed.It contained rich essential amino acids and useful mineral elements such as Mg, Zn.The contents of EPA & DHA in liver were rich. Conclusion L.wheeleri in Dapeng Bay had rich nutrient but toxin-free, can be considered as a safely consumble pufferfish species.
2004, 20(3): 307-308.
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Objective To learn the distribution feature of beta 3-adrenergic receptor β(3-AR)Trp64Arg mutation in school-aged children in Nanjing and its relationship with childhood obesity. Methods β3-AR Trp64Arg mutation was detected with the method of RFL P among 177 school children, their height and weight were measured. Results The mutative C allee was 0.24 for all the children, 0.226 for boys and 0.267 for girls.The frequency of C allee and the genotype Trp64Arg was higher in obese girls.The frequency of the genotype Trp64Arg was 70.3%, 71.4% for obese girls with obese parents or obese mothers.Body weight and BMI of the children with β3-AR Trp64Arg mutation were also higher.The risk for obesity in girls with β3-AR Trp64Arg mutation was 4.5. Conclusion There was distribution of β3-AR Trp64Arg mutation in school-aged children in Nanjing, especially in obese girls.It may be related to obesity.
2004, 20(3): 309-310.
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Objective To establish the method for the determination of biological essential elements K、Na、Mg、P、Cu、Fe、Mn、Zn in Fetal Cerebra. Methods The samples were digested by closed-vessel microwave HNO3+H2O2 dissolution system.The solution was directly analyzed by ICP-AES for the determination of K、Na、Mg、P、Cu、Fe、Mn、Zn. Results The detection limits of each element was between 0.007 mg/L and 0.1 mg/L.Standard linear comelationr≥0.999.Standard deviations were below 2%.Recoveries of spiked saniples were of the range 93.7% and 116.1%.Two standard reference materials nanlely "pig Liver GBW08551" and "Human Hair GBW07601" were used an quality control standards and the analysis results conformed well to the certified values. Conclusion The method was proved to be simple, rapid, accurate and reliable.And it has been applied to the analysis of the sample with satisfactory results.
2004, 20(3): 311-312.
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Objective To study the effects of plumbum(Ⅱ) or cadmium(Ⅱ) on DNA damage of germ cells of male mice in vitro. Methods The DNA damage was observed by means of SCGE assay. Results Different dose plumbum(Ⅱ) or cadmium(Ⅱ) could cause DNA damages in male mice germ cells and there was significant dose-effect relationship. Conclusion Plumbum(Ⅱ) or cadmium(Ⅱ) could cause DNA damage of germ cells in vitro.
2004, 20(3): 312-313.
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Objective To identify risk factors in patient with metabolic syndrome(MS). Methods A case-control study of 95 cases with MS and 130 control subjects were performed.Statistical analysis of univariate, logistic regression were used to identify risk factors of MS. Results Age, family history of diabetes and alcohol intake enter the logistic equation by statistic significance(P < 0.05).OR value of age was 1.15, OR value of family history of diabetes was 2.79, OR of alcohol intake was 4.05. Conclusion Age, family history of diabetes and alcohol intake were risk factors for MS.
2004, 20(3): 314-315.
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Objective To explore the preventive and therapeutic effect ofγ-interferon to experimental silicosis by studying the effect of γ-interferon on lung/body coefficient and hydroxyproline in rats exposed to quartz dust. Methods Female Wistar rats were exposed to lower dose(20 mg/rat)of quartz dust by intratracheal injection.Beginning from the second day, the experimenatal rats were treated withγ-interferon(1×105 IU/rat)daily.Rats were killed after 1 and 2 month.Changes of lung/body coefficient and lung hydroxyproline content were determined. Results The lung/body coefficient showed no significant difference between quartz control groups and treated groups.The lung hydroxyproline of treated groups were significantly lower than that of quartz control groups(P < 0.05). Conclusion γ-interferon could significanlty reduce lung hydroxyproline content of quartz exposed rats.This denoted thatγ-interferon may inhibit or delay early silicotic fibrosis.
2004, 20(3): 316-317.
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Objective To probe the laws of the cross-adapting response induced by low dose radiation and low concentration mitomycin C. Methods The human lymphocytes, bone marrow cells of mice were treated by low dose X-ray or low concentration mitomycin C before treated by high dose X-ray or high concentration mitomycin C.Some targets were observed, such as chromosome aberration of human lymphocytes, bone marrow cells of mice, micronuclear(MN), sister chromatied exchange(SCE). Results The targets of above all appeared cross-adapt response. Conclusion Cross-adapt response can be inducd by low dose radiation or by low concentration mitomycin C not only in vivo but also in vitro.
2004, 20(3): 318-319.
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Objective To explore components of organic chemicals and polluted levels of important organic materials in human body, evaluate its potential hazard on population health. Methods The venous blood specimens from 50 health women were collected.Qualitative analysis organic chemicals and quantitative detective important organic materials in venous blood by gas chromatography/mass spectrometer(GC/MS)after extraction with hexane. Results 158 kinds of organic materials were detected from all blood samples, a significantly high frequency of organic chemicals included 9-Octadecenoic acid(82%), heptadecene-(8)-carbonic acid(66%), di-n-butyl phthalate(62%), thiosulfuric acid(H2S2O3), S-(2-aminoethyl) ester(34%), 14-Bate-H-pregna(28%), et c.Di-n-butyl phthalate with highly checked out frequency and toxic effect was determined quantity. Conclusion Organic pollutants existed in human body and some of them were highly checked out.Di-n-butyl phthalate with highly checked out rate and highly concentration had reproductive developmental toxicity as prority control pollution in USEPA.It would bring about a potential adverse effect on population health(for example reproductive toxicity).It should cause our highly attention(special child-bearing period women).
2004, 20(3): 320-322.
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Objective To study the effects of Chlorfenapyr on DNA damage of spleen and liver cells in mice. Methods Chlorfenapyr was administrated to mice with 4.9, 9.8 and 19.6 mg/kg doses once by gavage, then the percentage of cells with comets and the DNA migration of spleen cells and liver cells were detected by single cell gel electrophoresis. Results Compared with the control group, the percentage of cells with comets and the DNA migration were increased significantly at all the three tested doses of the two kinds of tissue cells(P < 0.05-0.001);except for the DNA migration of spleen cells, the percentage of cells with comets of spleen cells and liver cells and the DNA migration of liver cells exhibited a significant linear dose-dependent increase(r values were 0.995, 0.9987 and 0.9999 respectively, P < 0.05);at the identical dosage criterion, the percentage of cells with comets of liver cells increased statistically at all the three tested doses as compared to the corresponding dose spleen cells groups(P < 0.01), so did the DNA migration of the 4.9 mg/kg dose of liver cells(P < 0.05). Conclusion Chlorfenapyr can induce the DNA damage of spleen cells and liver cells, and the effects on the DNA damage of liver cells is stronger than that of spleen cells.
2004, 20(3): 323-324.
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Objective To investigate the effect of exogenous DNA on the production of lipid peroxide(LPO)and the activities of superoxide dismutase(SOD)in aging rat's cardiac muscle, liver and kidney. Methods Twenty healthy spragueDawley(SD)male rats were chosen and randomly divided by abdominal cavity with 0, 3.0 mg of DNA preparation in every day for 22 days.All rats of each group were killed at the 23th days and their cardiac muscel, liver, kidney were collected and used in determining contents of LPO and activites of SOD. Results The contents of LPO was significantly lower in DNA group than in the control group.The activites of SOD were increased in DNA group. Conclusion DNA may have the effect on cleaning oxygen free radical and antioxidant action wich can delay the aging of cardiac muscle, liver and kidney.
2004, 20(3): 324-325.
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Objective To use daily records of local family planning practice and assess the perinatal mortality and its determinants in a less developed rual area. Methods The data of pregnancy related daily records provided by township family planing service stations were anlaysed. Results The perinatal mortality rate(PNMR)and stillbirth rate(SBR)in studied area were 69 and 24 per 1 000 births, respectively, and the early neonatal mortality rate(ENMR)was 46 per 1 000 live births.The male and female ENMR were 29 and 69 kper thousand live biths, respectively.The PNMR was associated with parity and townksipo economic level. Conclusion The PNMR in the studied county was higher than that in rural areas reported earlier.The results by parity and infant sex reflected the impact of the local economy and existing family planning policy on perinatal health.
2004, 20(3): 327-328.
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Objective To investigate dactylogram information of the hypoxia stress peptide(HSP)with the Nuclear Magnetic Resonance(NMR)and to establish the fast method for detecting the HSP to provid the new measure for screening the crowd easy to sickness. Methods To detect the blood from the wistar rats treated in the hypoxic environment and the mountain sickness patients with the NMR, and detect to the HSP with the reagent dispensed by ourselves, optimization the experiment condition. Results Character absorption cusp was exited at the definite wave-length tn the serum from the 104 wistar rats, but only two was exited in the control group(62 cases), there was significant difference between the two groups.The cases of the chemistry migration expressed high of the mountain sickness and the control group were 40(43)and 2(43), showing significant difference between the two groips(P < 0.01).The NMRIH character chemistry migration expressed both the group of the experiment of the mountain animal and the group of clinical patients.The reaction time and wave-length were 30min and 560nm in the method of fast detecting. Conclusion The HSP was exited in the body fluid of the mountain sickness patients, the chapter of the HSP was different from the other substance as known; The method of fast detecting can provid the new medilum to select the soldier for the army and to screen the easy-sickned crowd.
2004, 20(3): 334-335.
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Objective In order to assess the infection condition of HBV, HCV, HGV, HSV and CMY in Intravenous Drug Users(IVDUs). Methods It is detected that anti-HBV, HCY, HGV, HSV, CMY markers by ELISA in 406 sera and 102 normal controls.While we detected HCV RNA by RT-PCR and Fluorescence Quantitative PCR methods. Results Anti-HGV, HSV and CMV positive is 1.97%, 2.71% and 3.45% respctively, total HBV infection rate is 36.45%, HBsAg carrier 5.91%, HBeAg positive 3.96% in IVDUs.HBV infection rate is 17.65%, HBsAg carrier 0.98%, HBeAg positive 1.96% in the controls.Others are negative.The infection rates and coinfection rates are very prominent in the injector cousers of IVDUs(49.01%). Conclusion It indicates that IVDUs is high risk population of blood transmission virus infection, especially HCV infection.
2004, 20(3): 336-337.
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Objective To study the occurrence of depression symptoms and associated factors in middle school students of Chengdu urban area. Methods Multiple random sample method was used in the study.1 421 middle school students were sampled.Self-editted questionnare(including age, sex, family circumstance, experience in childhood, etc.)and Beck Depression Inventory(BDI)were used in the sampled students. Results The mean score of BDI was 10.98±7.39.The mean score of female was higher than that of male(P < 0.01).The most common depression symptoms included fatigue, guilt, depression mood, dissatisfaction, etc.The higher BDI scores were expressed in students of graduating class, parents' divorce family, remarried family, notharmony of family members, not reared by parents and violence or suicide behaviors in family members. Conclusion and teachers should discover and intervene the students' depression symptoms as early as possible.
2004, 20(3): 338-339.
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Objective A cross-sectional study for the knowledge of violence in hospital among doctors and nurses was conducted in order to provided the basis for prevention in the future. Methods 505 hospital workers were investigated with uniform questionnaire by cluster sampling. Results The study showed that the hospital workers knew something about the knowledge of hospital violence, but the general was low, only 36.4% subjects' answers were right.The following were dlought as main risk factors, such as the wrong induction of media, incoordination between doctor and patient, low Disposition of patients, and the malpractice. Conclusion In order to prevent it occurring, we should develop the right education, improving the relation between doctor and patient, rising the quality of health service.
2004, 20(3): 339-340.
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Objective To investigate the quality of life and its influential factors of urban poor people. Methods The quality of life of 237 poor people and 261 normal people living in the city of shenyang is assessing by the short form 36.The test and logistic regression are used to test the impacts of main influential factor. Results The quality of life of poverty group and control group were signifcantly different.The influential factor of physical health of poor people were age, sex, cost of medical treatment, debts, aimsgiving and sickness; p The influential factor of mental health of poor people were cost of medical treatment, cost of education, debts and aimsgiving.When years of poverty increase, the scores of domains decrease except RE. Conclusion The quality of life of urban poor people is lower than normal population significantly, particularly for poor people between 35 and 50 years old.The quality of life of poor people is influenced by age, sex, cost of medical treatment, debts, aimsgiving and sickness, cost of education.
2004, 20(3): 341-342.
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Objective To study the relationship between quality of life and satisfaction of life of middle-age intellectual. Methods The 463 middle2age intellectual aged 40-59 were measured by questionnaires and their physical examination was conducted.The data was analyzed with factor analysis and canonical correlation analysis. Results The relationship of quality of life and satisfaction of life of middle-age intellectual was positive correlation(r=0.674 8);Satisfaction of life was mostly showed by relation among people, family life, medical health care and body health. Conclusion The key to promote quality of life was to improve satifaction of life.
2004, 20(3): 351-352.
Abstract:
Objective To investigate early-breast-development girls social abilities, behavior problems and the related factors. Methods 43-early-breast development girls aged 6-11 were investigated with Achenbach Child Behavior Scale(for parents)and self-made questionnaire on childrens family environment.Another 160 girls were selected as control group, who were investigated with Achenbach only. Results The average scores of early breast development girls activity, sociality, and school achievements were 4.49, 4.83 and 5.46.There were no differences between the early breast development girls(23.3%)and controls(20.6%)on prevalence of behavior problem.The total score and the scores of delinquent behavior, aggressive behavior, cruel behavior of early breast development girls were significantly higher than controls(P < 0.05).The result indicated that the number of girl-friends was related with behavior problems of girls early breast development girls. Conclusion Early-breast-development girls behavior problems should not be ignored.The proper pubertal development education and comprehensive measures were necessary to prevent or to cure behavior problems of early breast development girls.
2004, 20(3): 355-356.
Abstract:
Objective The reactive oxygen species(ROS)production and A TPase in human bronchial epithelial cell line induced by cooking oil fume condensation were studied through measuring extra celluar and intracellular ROS levels including super oxide anions(2)and hydrogen peroxides(H2O2), and ATPase contents. Methods Sepectrometric method and fluorometric method were used to measure the ROS reduced by COFC.And the activities of ATPase assayed by biochemical method. Results The ROS correlated with the decrease of contents of A TPase. Conclusion Membrane ATPase activity was inhibited by ROS, especially Ca2+ATPase and Mg2+ATPase.
2004, 20(3): 363-364.
Abstract:
Objective To assess resistance risk to cypermethr in and predict the rate of r esistance development in housefly(Musca domestica). Methods Houseflies collected from natural group were cultured in laboratory.Resistant strain to cypermethrin in housefly was obtained while LD50 was deter mined with the method of topical application.Based on the method described by Tabashnik(1992), the realized heritability(h2)to cypermethrin in housefly was estimated and the rate of resistance development was predicted. Results Continuously selected with cypermethr in for 10 generations at the average survival percentage of 54.5% in laboratory, the strain of housefly had a 50-fold increase in resistance to cypermethrin.The realized heritability(h2)of resistance to cyper methrin in housefly was estimated to be 0.157 7.The rate of resistance development for 10 fold in the housefly was predicted based on the estimation of the realized heritabilit y(h2), and it only needs 2.2-4.8 generations under the selected pressure of mortality ranged 50%-90%. Conclusion The resistance to cypermethrin in housefly in creased rapidly and the realized heritability(h2)was stable in 10 generations.
2004, 20(3): 369-370.
Abstract:
Objective During the spreading of respiratory epidemic, to change all-air central air conditioning system in order to clean dust and sterilize air, and to reduce the second pollution and cut off the way of spreading virus. Methods Standing on the dust removing, the wet filler is used to be the function part of dust collection and sterilization. Results The wet dust collection with filler can usually remove the 25-40 percent of dust with a diameter of 1 micron, and 75-98 percent of 5 micron, but the pressure loss is 30-130 pascal. Conclusions The wet dust collection with filler can clean, sterilize, humidify and cool the air.It can replace the first dust collection in the traditional air conditioning, and it is important to stop the virsus spreading by the system, improve the air quality and save the energy.
2004, 20(3): 377-378.
Abstract:
Objective To study the change of age structure of popoulation and its relationship to development of health business in Henan Province. Methods To analyse the date of the 3rd, 4th and 5th overall survey of population by SAS software. Results The average increase rate of the population was 1.75% per year during 1982 and 1990, and it was only 0.65% during 1990 and 2000.The age median of population increased 10 years compared with that in 1982.The number of the elderly people aged over 60 years shared 10.17% of all, and the trend was ascending.The cities where lived about 17% people of all had more than 50% health resources of the total. Conclusion The age structure of population changed to the old, and the area health planning must be made immediately.