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2004 Vol. 20, No. 5

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Study on replication status of HBsAg positive pregnant women and newborns
SHI Xiao-hong, WANG Su-ping, LI Shu-zhen,
2004, 20(5): 513-514. doi: 10.11847/zgggws2004-20-05-01
Abstract(1135) HTML (247) PDF 820KB(52)
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  Objective   In order to explore the replication status of PBMC HBV of HBsAg positive pregnant women and their newborns.   Methods   151 pregnant women and 153 newborns were collected between 2001 June and 2002 December in Infectious Hospital of Taiyuan city. PBMC HBV DNA were deter mined by selected poly merase chain reaction (sPCR).   Results   15(9.93%) out of 151 HBsAg positive pregnant women had HBV cccDNA in their PBMC, 7(4.58%) out of 153 newborns had HBV cccDNA in their PBMC, 55(36.42%) out of 151 HBsAg positive pregnant women had HBV rcDNA in their PBMC, 35(22.88%) out of 153 new borns had HBV rcDNA in their PBMC.   Conclusion   PBMC was the replication site of HBV out of liver. PBMC HBV replicatlon of newborns was related to the replication of their mothers.
Obesity induced by high energy diet and its effects on lipid metabolism of rat
SUN Wen-guang, SUN Chang-hao
2004, 20(5): 514-515. doi: 10.11847/zgggws2004-20-05-02
Abstract(1044) HTML (224) PDF 332KB(44)
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  Objective   To study obesity induced by high energy diet and its effects on lipid metabolism of rat.   Methods   The rats were divided into two groups, one fed with basic diet and the other with high energy diet. At the end of the 7th week, different indexes were tested such as body weight, body fat content, blood lipid, leptin, SOD, MDA and GSH-Px.   Results   High energy diet induced obesity. Compared with basic diet group, the levels of triglyceride, cholesterol and leptin in serum increased significantly (P < 0.05 and P < 0.01). The activity of GSH-Px increased and SOD decreased significantly (P < 0.05), the level of MDA increased, but there was no significant diference between two groups (P > 0.05).   Conclusion   High energy diet induced obese rats to cause leptin resistance and the disorder of lipid metabolism, and made the activity of body antioxidation lower.
Relation between excessive iodine intake and mental development of children
ZHAO Jin-kou, ZHANG Qing-lan, SHANG Li,
2004, 20(5): 516-518. doi: 10.11847/zgggws2004-20-05-03
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  Objective   To describe the relationship of excessive iodine intake with intellectual development of children.   Methods   Iodine concentrations in drinking water and in urine were assessed in 13 townships in Feng, Pei and Tongshan counties of Jiangsu Province. Based on the median iodine concentration in drinking water, the townships were divided into six groups: water iodine < 100(A), 100-(B), 300-(C), 500-(D), 700-(E) and ≥900μg/L(F). IQ was measured. School children aged 8-15 years with a combined Raven test, weight, height, and urinary iodine concentration data were also collected and compared among 6 groups.   Results   For children' aged > 11 years, the mean IQ scores of group D(105.5), group E(105.2) and group F(102.3) were significantly lower (P < 0.01) than the mean IQ score of group A(108.9). IQ was related negatively to concentration of iodine in drinking water and in the urine(β=-0.803, β=-0.546 respectively). The IQ of children aged ≤11 years in group F was also lower than their counterparts in group A.   Conclusion   Excessive iodine intake appeared to impair cognitive development of schoolchildren.   Results   supported the premise that the maximum allowable iodine concentration in drinking water should be set at 300 or 800μg/L in urine.
2004, 20(5): 518-518. doi: 10.11847/zgggws2004-20-05-04
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Detetion of methicllin staphylococcus aureus and survey on staphylococcus aureus enterotoxin
LONG Jin, CHENG Qing, YU Shou-yi
2004, 20(5): 519-520. doi: 10.11847/zgggws2004-20-05-05
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  Objective   To evaluate of Latex fixation tests for detection of methicillin resistant staphylococcus aureus and to detect of staphylococcus aureus enterotoxin.   Methods   130 staphylococcus aureus isolates extracted clinically were classified into methicllin resistant staphylococcus aureus (MRSA) and methicllin sensitive staphylococcus aureus (MSSA) based on the antibiotics sensitive test, A latex fixation tests to detect penicillin-bindingprotein 2a, was compared with the MecA PCR for the detection of methicllin resistance in staphylococcus aureus and the detection of staphylococcus aureus enterotoxin by Enzyme linked immunosorbent assay test.   Results   Of the 65 MecA gene positive isolates, 62 were positive by the latex fixation, and all of the 63MecA gene negative strains were negative by latex fixation tests. MRSA with enterotoxin were 67 strains, by comparison, MSSA were 19 strains.   Conclusion   Latex fixation test was simple, fast and accurate for detection of MRSA and great emphasis should be on the detection of staphylococcus aureus enterotoxin in clinical laboratory.
Case-control study on risk factors of childhood cerebral palsy
QIU Hong-bin, WANG Zhi-guo, WANG Bao-lai,
2004, 20(5): 520-521. doi: 10.11847/zgggws2004-20-05-06
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  Objective   To indentify possible risk factors of cerebral palsy(CP)in children.   Methods   A hospital-based 1: 2 matched case-control study was carried out in 103 children with CP and 206 controls individually matched for sex and age were selected for each case. The data were analyzd by conditional Logistic regression.   Results   Eleven risk factors were suggested from univariate analysis, namely mother occupation, abnormal perinatal history, maternal malnutrition and anemia during pregnancy, premature birth, lower birth weight, lower apgar score, neonatal diseases. The risk factors confirmed by multivariate analysis were mother occupation, premature birth, neonatal asphyxia.   Conclusion   Relevant risk factors of CP could be seen primarily in gestational and perinatal periods. Their prevention could reduce the incidene of cerebral palsy.
Effects of estradiol on EA As release by deltamethrin in glial cell
CHEN Liang, SHI Nian, WU You-tong,
2004, 20(5): 522-523. doi: 10.11847/zgggws2004-20-05-07
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  Objective   Using rat primary glial cells as model in vitro to investigate the effects of estrogen on the release of EAAs by deltamethrin.   Methods   Using HPLC to detect the EAAs release of different 17β-estradiol dose level (10-5, 10-8, 10-11 mol/L) in the glial cells treated with deltamethrin. Meanwhile, the estrogen receptor(ER)antagonist tamoxifen was used to investigate the mechanism of estrogen.   Results   The Glu release in the glial cells was increased significantly by 2×10-5 mol/L deltamethrin, and 10-8 estradiol can partly block this release. The effect of 10-8mol/L estradiol can not be antagonisted by tamoxifen.   Conclusion   Estradiol showed a neuroprotective effect on the release of Glu by deltamethrin in the glial cells.
Effect of nanosized silicon dioxide on expressions of interleukin-4 and fibroblast growth factor-b in lungs of rat
JIANG You-hong, DONG Jing, CHEN Ying,
2004, 20(5): 524-525. doi: 10.11847/zgggws2004-20-05-08
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  Objective   To study the effect of nanosized silicon dioxide on expression of IL-4 (interleukin-4) and FGF-b (fibroblast growth factor-b) in silicotic lungs of rats.   Methods   Female Wistar rats were exposed to lower dosage (20mg/rat) of nanosized silicon dioxide and normal silicon dioxide by intratracneal injection. Rats were killed on the 1st month and 2nd month respectively to observe the pathological changes of silicotic rats by means of HE and VG dyeing and expression of IL-4 and FGF-b by means of immunohistochemical technique.   Results   (1) Pathological examination showed that pulmonary fibrosis lesions of rat lung were much lighter in nanosized silicon dioxide groups than that of normal silicon dioxide groups. (2) The expression of IL-4 and TGF-β1 in nanosized silicon dioxide groups was higher than control groups and lighter than normal silicon dioxide groups. The expression of IL-4 was heavier on the 1st month than on the 2nd month both in nanosized silicon dioxide groups and normal silicon dioxide groups. And the expression of FGF-b was similar to both the first and the second month and in both normal silicon dioxide groups and nanosized silicon dioxide groups.   Conclusion   The effect of pulmonary fibrosis lesions was light in nanosized silicon dioxide. The expressions of IL-4 in rats lungs were low. FGF-b may have no effect on the fibrosis of the lungs of rats.
Cell apoptosis induced by ceramide in human colon cancer cells
LI Bai-xiang, ZHANG Tao
2004, 20(5): 526-527. doi: 10.11847/zgggws2004-20-05-09
Abstract(779) HTML (256) PDF 329KB(63)
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  Objective   The effect of cell apoptosis induced by ceramide was investigated in human conlon cells(HT29).   Methods   The curve of cell growth(MTT test)was used. Cell apoptosis was detected under fluorescent and electronic microscope and expressed bcl-2, Fas, p53 protein.   Results   Ceramide inhibited remarkably the cell growth and induced cell apoptosis. The expression of Fas protein in HT-29 cells increased as the dose increased and the expression of p53 protein decreased as the dose increased.   Conclusion   Ceramide can significantly inhibit the growth of HT-29 cells and induce apoptosis.
Research on solar-UV individual exposure of undergraduate in Shenyang
YU Dao-jun, LIU Yang, Masaji Ono,
2004, 20(5): 528-529. doi: 10.11847/zgggws2004-20-05-10
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  Objective   To explore personal exposure to solar-UV radiation of underg raduates in Shenyang.   Methods   Personal exposure to solar-UV radiation of under graduate in Shenyang in four seasons was measured with UVR individual monitors. And at the same time the measurement of environmental ultraviolet radiation dose was carried out.   Results   The research show ed the distribution of individual UVR exposure of each period in daytime, of daily cumulative amount and in different season. The percent personal ambient UVR exposures was less than 1.0 percent.   Conclusion   The distribution of UVR exposure of underg raduates in daytime was corresponded with outdoor-time on the whole. Individual variation was mostly caused by personal behavior. Different season had great effect on UVR exposure. The differ ence between personal UVR exposure and environmental dose was large.
Effect of fluoride in drinking water on children's dental caries
WANG Yang, WANG Cai-sheng, XIANG Quan-yong,
2004, 20(5): 530-531. doi: 10.11847/zgggws2004-20-05-11
Abstract(893) HTML (231) PDF 334KB(32)
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  Objective   To study the relationship between the fluoride in drinking water and the prevalence of children's dental caries, and try to find the suitable concentration of fluoride in drinking water for dental caries prevention.   Methods   Epidemiologic methods were used to investigate the prevalence of children's dental caries in Xinhua (drinking water fluoride mean and range in mg/L: 0.36±0.11, 0.19-0.76, children aged 8-13 years old, 290) and Wamiao (2.46±0.80, 0.574.50, 222 children aged 8-14 years old). Children were divided into nine subgrouups according to the drinking water fluoride: < 0.5, 0.5-, 1.0-, 1.5-, 2.0-, 2.5-, 3.0-, 3.5 and≥4.0 mg/L. To explore the dose-response relationship between the drinking water fluoride and the prevalence of children's dental caries, and calculate the BMDL(the LowerBound Confidence Limit on BenchMark Dose).   Results   As the fluoide in drinking water increased the prevalence of children's dental caries reduced gradually when the fluoride in drinking water below 2.72 mg/L, but there were positive relationship when drinking water fluoride over 2.72 mg/L. The dose-response relationship between the fluoride in drinking water and the prevalence of children's dental caries was a U-shape. Considering the complicated cause of dental caries and other sources of dietary fluoride, the RfD (reference value dose) were calculated to be 1.55 mg/L and 1.29 mg/L.   Conclusion   The suitable concentration of fluoride in drinking water for prevention dental caries was 1.0-1.5 mg/L in the investigated area.
Investigation on dengue virus from Aedes albopictus field in Guizhou
SHU Li-ping, ZUO Li, HAO Mu,
2004, 20(5): 532-534. doi: 10.11847/zgggws2004-20-05-12
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  Objective   To study molecular epidemiology of dengue virus from Aedes albopictus field in Guizhou province.   Methods   18 different population of Aedes albopictus larvals were collected in Guizhou. The total RNA from mosquito were extracted. The viral NS1 gene fragment were amplified with reverse transcriptase polymerase chain reaction(RT-PCR). And the products were digested with Mav I for typing. Dengue virus in mosquito was isolated with C6/36 cells. Then the viral antigen was detected by indirect immunofluorescence assay(IFA).   Results   One strain of dengue virus was isolated with C6/36 cells from field Aedes albopictus in Mawei town. It was identified as dengue virus type 2 by IFA with monoclonal antibody and RT-PCR. Dengue virus type 2 or 4 RNA were detected in A edes albopict us field collected from Kaili, Huangguoshu and Zhenyuan areas in Guizhou, respectively.   Conclusion   These findings highlighted the existence of a silent epidemic in Guizhou.
2004, 20(5): 534-534. doi: 10.11847/zgggws2004-20-05-13
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Detection of p16 protein of colorectal cancer
CHENG Su-qin, LI Jian-biao
2004, 20(5): 535-536. doi: 10.11847/zgggws2004-20-05-14
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  Objective   To detect the expressio n of p16 protein in colorectal cancer and its significance in clinical application.   Methods   Immuno histo chemistry S-P was used to detect the p16 protein of colorectal cancer.   Results   The positive rate of p16 protein was 75.7% in 37 cases, In high, mediate and low differentiated colorectal cancers was 87%, 17%, respectively, the positive rate (100%) in colonic tumor was higher than the recta's tumor, and then between them exist the evidence differences. In Dukes stage, the ABand CD positive rate was 81% and 69%, respectively. The lymph node without metastasis was higher (positive rate 81%) than the lymph node with metastasis's positive rate 69%.   Conclusion   The p16 protein was associated with the occurrence and development of colorectal cancer, and the same as the histology classification, Dukes stages and whether metastasis of lymph node. The p16 protein can be considered the indication of nature cause of disease and prognosis of colorectal cancers in the future clinical applications.
Evaluation on SF-36 to assess quality of life of patients with pulmonary tubercolosis
DUAN Qiong-hong, Dick Chamla, NIE Shao-fa,
2004, 20(5): 537-539. doi: 10.11847/zgggws2004-20-05-15
Abstract(1137) HTML (276) PDF 472KB(66)
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  Objective   To evaluate the reliability, validity and sensitivity of Chinese version SF-36 to assess the quality of life of PTB patients.   Methods   Subjects were interviewed by using Chinese version of SF-36. Validity of the scores was tested by factor analysis and reliability coefficients were estimated by Cronchbach's a with 0.7 as a standard. Sensitivity was evaluated by comparison of PTB cases and controls.   Results   Scores of SF-36 in both groups were valid(The patterns of correlations between 8 scales of SF-36 and rotated two factors supported it)with reliabilit y coefficients as estimated by Cronchbach's a above 0.7. Scores of SF-36 in both groups had high sensitivity(all scales of TB patients were lower than that of the control).   Conclusion   Chinese version of SF-36 was a valid and reliable tool for assessment of quality of life, and cultural diversity might require slight modifications of SF-36. There was a need of large scale general health survey to produce normative data for Chinese population.
2004, 20(5): 539-539. doi: 10.11847/zgggws2004-20-05-16
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Expression of recombinant hARRG cDNA in E.coli and purification of hARRG protein
HE Ling, PAN Ze-min, TAN Xiao-hua,
2004, 20(5): 540-541. doi: 10.11847/zgggws2004-20-05-17
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  Objective   To construct expression vector of the recombinanthuman arsenic resistance related gene(hAR-RG), induce its expression in DE3 and isolate and purify expression product, for studying the physiochemistry characteristic, function and immune activity of the protein, and further researching the arsenic resistant effects of human.   Methods   hARRG cDNA was subcloned into prokaryotic expression vector Pet11C. The recombinant protein expression was induced by IPTG, then, the protein was purified by anions Ion-exchange column Sepharose and examined by SDS-PA GE gel.   Results   hARRGcDNA was successfully subcloned into prokaryotic expression vector Pet11C and expressed in E. coli and the protein was purified by anions Ion-exchange column successfully.   Conclusion   Pet11C excpression vector containing hARRG cDNA wassuccessfully constructed, the cell DE3 transformed with expression vector capable of expression the gene and a hARRG protein could be purified by anions Ion-exchange column Sepharose.
Effect of dietary fatty acids on fatty acids composition of serum phospholipids in diabetes mellitus model rats
SHI Ji-hong, SHI Hong-li, FANG Jin-chong,
2004, 20(5): 542-544. doi: 10.11847/zgggws2004-20-05-18
Abstract(726) HTML (195) PDF 270KB(32)
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  Objective   To investigate the influence of dietary n-3 compared with n-6 PUFA on the FA composition of S-PL and glucose metabolism in OLETF rats, a model of spontaneous type 2 diabetes mellitus.   Methods   20 male OLETF rats of 14-week age were divided into 2 groups randomly. Isocaloric diets contained either n-6 PUFA 141.6 mol/(100 mol·fats)2 or n-3 PUFA 1 20.4 mol/(100 mol·fats)2 buthad identical contents of SFA, MUFA and PUFA. An OGTT and measur ement of insulin level was performed at 24 weeks of age after 10-week treatment. The FA composition of S-PL in the two groups of rats was observed as well.   Results   The food intake and bodyweight gain were comparable in n-6 and n3 groups. The results of OGTT and measurement of serum insulin level showed that the n-3 diethad a more beneficial effect on insulin sensitivity than the n-6 diet. The S-PL in the rats of the n-3 diethad higher contents of 18: 1(n-9), total MUF As, 18: 2(n-6), n-3 series PUFAs and lower contents of 20: 4(n-6), total n-6 PUFAs than in the n-6 diet group.   Conclusion   The n-3 diethad a more beneficial effect on glucose metabolism than the n-6 diet. The FA composition of S-PL was influenced by the dietary fats but not a direct reflection of dietary FA composit ion in OLETF rats.
2004, 20(5): 544-545. doi: 10.11847/zgggws2004-20-05-19
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Study on microecoenvironment of legionella pneumophila with its proliferative speed and propagationg in air-conditioning cooling water
GUO Chang-yi, SU Jin, RUAN Su-yun,
2004, 20(5): 546-549. doi: 10.11847/zgggws2004-20-05-20
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  Objective   To explore the cause that chlorodisinfectant unable to control proliferation and propagationg of legionella pneu mophila f or a long time. It is to provide guide for seeking the way that can control proliferation and propagationg of legionella pneumophila in air-conditioning cooling water for a long time.   Methods   By periodically detecting increase or reduce of legionella pneumophila and concent ration of total organic carbon(TOC), No3--N, total bacterial member and alga in 7 air-conditioning cooling water and constituting ecopatterns of legionella pneumophila as well as tesing legionella pneu mophila in air at the same time, proliferation and propagationg of legionella pneu mophila were investigated in air-conditioning cooling water.   Results   The appear or disappear and raise or down of legionella pneumophila member has mainly relation to water temperature, total organic carbon(TOC), No3--N, and alga member(Raphidiopsis, Pediastrum and Freubaria). In the best ecoenvironment coped from air-conditioning cooling water, proliferative speed of legionella pneumophila can attain to a fold as an hour and keep the multiplicative proliferation in this experimentally 3 days. When legionella pneu mophila member in the water attained to 4.2×104/ml, legionella pneumophila was tested in the air 30 centimetre above the water. When legionella pneumophila just was given, its proliferative speed also attained to a fold as an hour in the ecoenvironment of alga member reduce but proliferative speed was more and more down. After 3 days legionella pneumophila member in the ecoenvironment of alga member reduce only was 1/160 as that member in the ecoenvironment of alga member increase.   Conclusion   Legionella pneumophila in the air-conditioning cooling water below 20℃ was rather liveless than rest. The proliferative speed of legionella pneumophila was significanly related with alga member, total organic carbon (TOC)and No3--N besides temperature. It might be a fundamentally controlling legionnaires'disease outbreak method to destroy legionella pneumophila ecoenvironment and reduced its proliferative speed to cut off its propagationg among cooling towers by a certain rate removing alga, total organic carbon(TOC)and No3--N in air-conditioning cooling water.
2004, 20(5): 549-549. doi: 10.11847/zgggws2004-20-05-21
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Cross-section study on effect of smoking, drinking and other risk factors on stroke and its subtypes in elders
CHANG Qing, HE Yao, NI Bin,
2004, 20(5): 550-551. doi: 10.11847/zgggws2004-20-05-22
Abstract(1182) HTML (253) PDF 337KB(68)
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  Objective   To study the relationship of smoking, drinking and other risk factors with stroke and its subtypes in elders in Beijing, China.   Methods   The sutdy was a cross-section study including 2 096 old people in a community in Beijing by means of cluster sampling. Trained investigators finished the question-papers by face to face investigation and SPSS 1115 was used to analyze the data. There were 2 096 subjects.   Results   In univariate analyses, age, hypertension, course of hypertension, smoking, coronary heart disease, diabetes mellitus, hyperglycemia、HDL and the family history of stroke were risk factors to strok (P < 0.05). Smoking increased the risk of total stroke, OR(95% CI)=1.17(1.01-1.35), while drinking decreased the risk of total stroke and ischemic stroke in the male, 0.69(0.51-0.94) and 0.71(0.52-0.98) respectively. Multivariate analyses showed after adjusting for potential confounders(body mass index, passive smoking, history of coronary heart disease, diabetes mellitus, hyperlipemia and heart disease etc), hypertension increased the risk of ischemic, hemorrhagic and total stroke and smoking increased the risk of total stroke and hemorrhagic stroke, 1.19(1.02-1.39)and 1.53 (1.03-2.27) respectively, while drinking still decreased the risk of total stroke (OR=0.78, 95% CI=0.61 to 0.99).   Conclusion   Smoking was the risk factor to ischemic and total stroke, while moderate drinking reduced the risk.
Study on serum zinc level and relevant factors among preschool children
ZHOU Li, LIU Xiao-li, DUAN Li-hua,
2004, 20(5): 552-553. doi: 10.11847/zgggws2004-20-05-23
Abstract(715) HTML (179) PDF 309KB(43)
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  Objective   To investigate serum zinc level and relevant factors among preschool children in Shenzhen city, and to provide scientific basis of making policy for children health protection.   Methods   300 preschool children from 3 representative infants'schools in Shenzhen city were selected by several stages cluster sampling method. The questionnaire survey was conducted and the nutrilites of serum zinc was also determined in the laboratory. The descriptive analysis and Logistic Regression Analysis were performed by SPSS10.0.   Results   (1) The serum zinc level of Shenzhen preschool children was as follows: the median was 18.25μmol/L; the minimum was 3.62μmol/L; the maximum was 60.85μmol/L. There was nonsignificant difference between males and females(t=1.721, P=0.086);There was non-significant difference among different ages(F=0.95, P=0.417). (2)There were 5.32% children whose serum zinc was lower than normal level, and there was non-significant difference between males and females(χ2=1.035 3, P=0.309). (3)The three relevant factors on the serum zinc status: children supplementary zinc and mother's education were protective factors; having suffering respiratory diseases in the latest two months was risk factors.   Conclusion   The serum zinc deficiency existed among preschool children in Shenzhen, which was related to several factors, and lower than other areas in China.
2004, 20(5): 553-553. doi: 10.11847/zgggws2004-20-05-24
Abstract(683) HTML (217) PDF 69KB(19)
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Effect of taurine on retinal ultrastructure of rats with early-stage diabetic retina
XU Zhao-xia, MI Man-tian, XU Hong-xia
2004, 20(5): 554-555. doi: 10.11847/zgggws2004-20-05-25
Abstract(830) HTML (295) PDF 211KB(33)
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  Objective   To observe retinopathy of diabetic rats, and to investigate the effect of taurine on ultrastructure of ratsith early-stage diabetic retinopathy.   Methods   50 SD rats were chosen and divided at random into normal group (CON), one-month diabetic group(DM1), one-month taurine-treated diabetic group(DM1+T), three-month diabetic group (DM3), three-month taurine-treated diabetic group. Diabetic rat was induced by injected streptozocin into abdominal cavity once. Diabetic rats were treatedith taurine that were mixedith the food. The ratsere sacrificed at the time of one month, three months after the model being created. Retinal tissues of ratsere observedith optical microscope and transmission electronic micoscope.   Results   Taurine can effectively protect retinal capillaries and improve optical receptor functions.
Study on immunoassay for ochratoxin A
JIANG Tao, LI Feng-qing, WANG Huan-yu,
2004, 20(5): 556-558. doi: 10.11847/zgggws2004-20-05-26
Abstract(1034) HTML (276) PDF 488KB(85)
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  Objective   To develop a sensitive, specific and rapid enzyme-linked immunosorbent assay for detection of ochratoxin A(OA). To develop rapid detection kits that possessing patent of our country.   Methods   To produced hybridoma cell lines excreting monoclonal antibodies against OA by using B cell hybridom technique. And to obtain the monclonal antibodies against OA.   Results   The indirect competitive inhibition enzyme-linked immunosorbant assay(ELISA)was developed for detection OA in rice and wheat. The limit of detection concentration of OA was 0.5 ng/ml. The linear range of standard curve was between 2-500 ng/ml, the linear equation was Y=-0.272X+1.07(r=0.997 8). The recovery of OA was between 79.0%-119.7%. The OA contamination level of rice and wheat that were sold in Beijing was surveyed by using the method. Contamination rate of wheat was 60.71%, the maximum level was 8.26μg/kg; the contamination rate of rice as 17.86%, the maximum level was 3.44μg/kg.   Conclusion   The detection method was simple, fast sensitive and can meet the demand of practical work.
2004, 20(5): 558-558. doi: 10.11847/zgggws2004-20-05-27
Abstract(819) HTML (208) PDF 131KB(21)
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Estrogen receptor competitive binding assay of bisphenol A and para-nonylphenol
HUANG Yi-na, CHENG Wei-bo, XU Pei-yu,
2004, 20(5): 559-561. doi: 10.11847/zgggws2004-20-05-28
Abstract(1109) HTML (327) PDF 466KB(46)
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  Objective   The estrogen-like activity of bisphenol A and p-nonylphenol was examined with the estrogen receptor competitive binding assay.   Methods   Adulthealthy female SD rat were ovarietomized and sacrificed 2 weeks later. Cytosol estrogen receptor were abstracted from the uteri. Aliquots of 150μg cytosol protein were added with 1μCi/mol 3 HE2 50μl and increasing concentrations of unlabeled E2, BPA and p-NP, the total volume of reponse was 200μl. The mixtures were incubated at 4℃ 16-18 hours. DCC methods removed free labeled-ligand. At last, the radioactivity was measured.   Results   The percent inhibitory values(IC50)of E2 was 1×10-9 mol/L. BPA and p-NP can inhibit of 3 H-E2 binding to rat uterine cycotol estrogen receptor with an IC50 of 2.08×10-5 mol/L and 2.66×10-5 mol/L, respectively. The relative binding affinity(RBA)of BPA and p-NP were 0.004 8 and 0.003 8, respectively. The binding capacity of BPA to ER was bigger than that of p-NP.   Conclusion   Binding to ER maybe was the mechanism that BPA and p-N P mimicked estrogen-like activity.
2004, 20(5): 561-561. doi: 10.11847/zgggws2004-20-05-29
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Study on reproductive toxicity of subchronic cadmium exposure in male rats
WANG Wen-xiang, LIAO Hui-zhen, CAO Jian-ping,
2004, 20(5): 562-563. doi: 10.11847/zgggws2004-20-05-30
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  Objective   To explore the effects of subchronic cadmium exposure on male sexual systems.   Methods   24 male rats of SD-strain were divided into four groups.0, 0.1, 0.2 and 0.4 mg Cd2+ /(kg·bw)were injected subcutaneously in different groups for 5 weeks. After the exposure, the rats were sacrificed and the testis, epididymis were separated to detect.   Results   The testis tissue coefficient increased in the high dose group; sperm concentration, viability decreased, while sperm malformation rate increased in every dose groups. The hemoglobin, MDA contents increased significantly in the middle and high dose groups, but the content of MT increased only in high dose group.   Conclusion   Subchronic cadmium exposure can induce male sexual glands injury, especially to the testis. The mechanism may relate to peroxiodative injury, the lack of protection effect of MT.
2004, 20(5): 563-563. doi: 10.11847/zgggws2004-20-05-31
Abstract(646) HTML (185) PDF 129KB(29)
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Study on radioprotective effect of bacillus subtilis
YAN Yan, XU Jian-hua, YANG Fei
2004, 20(5): 564-565. doi: 10.11847/zgggws2004-20-05-32
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Abstract:
  Objective   To research the radioprotective effect of bacillus subtilison 60Co-γ-ray-induced injury.   Methods   Mice were treated with three different dosesof bacillus subtilis in 60 days. All groups were irradiated once with 60Co γ-ray on thirtieth-day, and then the difference in survival rate, average survival time, the changes in white blood cells, quantity of DNA in marrow cell and serum hemolysin were observed.   Results   The 30-day survival rate, average survival time, the WBC counts, quantity of DNA in marrow cell and serum hemolysin of Bacillus subtilis-protected mice were all much higher than those of the control group.   Conclusion   Bacillus subtilis had significant anti-radiation effect.
Restraining effect of ebselen on peroxidizing damage caused by alcohol to rat
ZHANG Jing, ZHANG Hong-yu, LIN Kai,
2004, 20(5): 565-566. doi: 10.11847/zgggws2004-20-05-33
Abstract(763) HTML (220) PDF 201KB(26)
Abstract:
  Objective   To realize the restraining effect of the synthesized ebselen(Ebs)on the peroxidizing damage.   Methods   5.0 g/(kg·d)of alcohol and 0.5-25.0 mg/(kg·d)of Ebs were given to the rats'stomach, and the activity of the glutathione peroxidase(GPx)in blood plasma and the amount of the lipid peroxide(LPO)in blood serum and the ratio were observed one time respectively at the end of the third week and the sixth week.   Results   Compared with the alcohol group, the GPx's activity of the Ebs group whose dose was over 5.0 mg/kg increased and the amount of LPO decreased at the end of the third week, the GPx's activity of the Ebs whose dose was over 5.0 mg/kg and the change of the amount of LPOgroup had the dose-response relatio n significantly at the end of the sixth week, the ratio between the GPx and LPO increased apparently.   Conclusion   The synthesized Ebs can restrain the oxidizing damage, and the effective dose was 5.0 g/(kg·d)of Ebs(3w)and 0.5 g/(kg·d)of Ebs(6 w).
2004, 20(5): 567-568. doi: 10.11847/zgggws2004-20-05-34
Abstract(759) HTML (210) PDF 338KB(21)
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2004, 20(5): 568-568. doi: 10.11847/zgggws2004-20-05-35
Abstract(802) HTML (232) PDF 216KB(26)
Abstract:
2004, 20(5): 569-570.
Abstract(900) HTML (253) PDF 202KB(40)
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Effects of Se on COX-2 mRNA expression
WANG Shu-ran, YANG Bao-feng, SUN Chang-hao,
2004, 20(5): 570-571.
Abstract(733) HTML (206) PDF 373KB(28)
Abstract:
  Objective   To investigate effects of Se, in the form of sodium selenite, on COX-2 mRNA expression, a cancer close related gene.   Methods   The amount of COX-2 mRNA of HT-29 cells, which were cultured in DMEM containing the concentration of 250, 500 and 1 000 nmol/L Se for 4, 8 and 24 h, was detected by real time PCR.   Results   The expression of COX-2 mRNA were down regulated significantly by 500 and 1 000 nmol/L Se for 24 hours.   Conclusion   Se had chemoprotective effect on human colon cancer through down regulating COX-2 gene expression and did good to man's suitable diet and offer a background for develoing Se anticancer drug.
2004, 20(5): 572-573.
Abstract(573) HTML (184) PDF 321KB(44)
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Survey of knowledge, attitude, behavior and practice related to STI/HIV among male homosexuality in Shenyang
GU Yuan, QU Peng, XU Lu,
2004, 20(5): 573-574.
Abstract(804) HTML (219) PDF 347KB(55)
Abstract:
  Objective   To investigate the KABP related to STI/HIV and describe the high risk behaviors that can cause AIDS epidemic among men who have sex with men in Shenyang.   Methods   342 male homosexuality were surveyed in the bars using copies of a questionnaire and conducted voluntary test.   Results   Most of the participants were well educated, but had poor knowledge of STI/HIV, and they obtained the knowledge by watching TV or reading newspapers or through internet.59.1% were tested for HIV antibody and syphilis, 2 MSM were reported HIV antibody positive(1%)syphilis infected rate were 20.8%.11.7% participants considered themselves had high criticality, and 47.1% reckoned maybe had half possibility of infection.The median age of the first sex was 18 years, average number of male sex partners was 4.4, and 36.3% of the participants had female in the past six months.91.5% had unprotected anal intercourse with male partners within the nearest six months.Only 11.1% of them reported consistent condom use with their male sex partners.The main reasons of not using condom were unlike or unnecessary.   Conclusion   High-risk sexual behaviors were ubiquitous among MSM, poor knowledge of STI/HIV and low rate of condom using made it feasible occurring potential HIV epidemic in this population.It was imperative to carry out targeted interventions for MSM to turne the tide.
2004, 20(5): 575-576.
Abstract(691) HTML (198) PDF 333KB(25)
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2004, 20(5): 576-576.
Abstract(494) HTML (192) PDF 200KB(40)
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2004, 20(5): 577-577.
Abstract(742) HTML (237) PDF 134KB(31)
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Survey on reproductive tract infection and intervention by family planning system in Beijing
ZHANG Li-jie, CEN Yang, ZHOU Da-chen,
2004, 20(5): 578-579.
Abstract(752) HTML (182) PDF 190KB(43)
Abstract:
  Objective   In order to make plans foreffective intervention measures, to survey the capacity of developing reproductive tract infection(RTI)intervention in county level family planning(FP)system of Beijing city.   Methods   A crosssectional study was conducted to investigate the FP agencies and the staff of county level in Beijing city with standard questionnaires.   Results   86% county level FP agencies in Beijing had propaganda equipments.86% counties in Beijing could develop diagnose and threat trichomonas, candidal vaginitis and 71% counties could diagnose and treat bacterial vaginitis.All the counties were not capable of diagnosing and treating other sexual transmitted diseases.FP staff grasped some basic know ledge on RTI which was the basic to accept further training on RTI intervention.they were willing to develop RTI intervention.   Conclusion   The existing equipments and technique provided basic to develop RTI intervention.Expanding the service and promoting the knowledge and skill among FP staff were necessary for further developing RTI prevention.
2004, 20(5): 580-581.
Abstract(730) HTML (207) PDF 185KB(37)
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2004, 20(5): 581-581.
Abstract(491) HTML (193) PDF 121KB(20)
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2004, 20(5): 582-583.
Abstract(641) HTML (211) PDF 1380KB(40)
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Survey on mother-to-child transmission of HIV and TTV
DENG Li-ping, GUI Xi-en, WANG Xi
2004, 20(5): 583-584.
Abstract(714) HTML (307) PDF 200KB(27)
Abstract:
  Objective   To estimate the mother-to-child transmission of HIV and TTV in highly epidemic rural area of AIDS.   Methods   228 high-risk women of childbearing age and 180 children aged 0-14 years were investigated.their medical histories of blood donation or transfusion are collected, blood samples are taken and serum are separated for anti-HIV and anti-TTV test.   Results   39 HIV infected cases are found among 103 children who are born by HIV-positive mother after 1993.the number of children infected with HIV through vertical transmission increases from 1993 to 2001.5 antiTTV is positive among 76 children who born by anti-TTV positive mothers.   Conclusion   Mother-to-child transmission is one tranmisson route of HIV and TTV in highly epidemic rural area of AIDS.Vertical transmission of HIVis the major route for children with HIV/AIDS.the main reason leading to children HIV infection is the lack of prenatal HIV counseling and testing for the high-risk women of childbearing age and lake of interventions.the counter measures must be taken to control the further transmission of AIDS in order to protect the health of women and children in highly epidemic areas of AIDS.
Meta-analysis on risk factors of osteoporosis
PENG Ji, LIANG Yuan, LU Zhu-xun
2004, 20(5): 585-586.
Abstract(878) HTML (340) PDF 335KB(149)
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  Objective   To provide evidence for osteoporosis(OP)prevention and control though probing the main risk factors related to the incidence of OP.   Methods   The result of 12 epidemiological studies on risk factors of OP from 1993 to 2003 were analyzed by Meta analysis, and odds ratio(OR)was the effect index.The number of case and control were 1 228 and 1 528, respectively.   Results   The pooled OR value are agedness(3.56), insufficient physical activity(2.83), low level dietary calcium(2.33), BMI under 20(2.06), smoking(1.98)and alcohol consumption(1.70).   Conclusion   Changing the bad living habit and increasing dietary nutrient can make for prevention of OP incidence.
2004, 20(5): 586-586.
Abstract(627) HTML (196) PDF 121KB(22)
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2004, 20(5): 587-587.
Abstract(636) HTML (188) PDF 190KB(28)
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2004, 20(5): 588-589.
Abstract(594) HTML (172) PDF 197KB(19)
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2004, 20(5): 589-589.
Abstract(580) HTML (225) PDF 193KB(28)
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2004, 20(5): 590-591.
Abstract(740) HTML (266) PDF 312KB(20)
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2004, 20(5): 591-591.
Abstract(671) HTML (171) PDF 68KB(24)
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2004, 20(5): 592-593.
Abstract(587) HTML (190) PDF 185KB(32)
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2004, 20(5): 593-593.
Abstract(1229) HTML (376) PDF 116KB(21)
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2004, 20(5): 594-594.
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2004, 20(5): 595-596.
Abstract(653) HTML (240) PDF 309KB(40)
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Effects of supplementary L-arginine on thymus and spleen in heat-stressed rats
ZHANG Yun-shan, LUO Hai-ji, JI Yan-hong,
2004, 20(5): 596-597.
Abstract(655) HTML (238) PDF 385KB(28)
Abstract:
  Objective   To inquire into the effects of heat stress on the body weight, thymus indexes, spleen indexes, respiration, rectal temperature and the protection function of supplementary L-Arginine(L-Arg)on heat-stressed rats.   Methods   According to the factorial experiment design for three factors, hebetic male Sprague Dawley rats were randomly divided into A, B, C groups.The relevant indexes change such as the thymus index were observed in SD rats which were exposed under normal temperature and different hyperthermia with supplied different concentration L-Arginine by gavage.   Results   In normal temperature, the body weight, thymus and spleen indexes increase of the groups supplemented with L-Arginine were higher than those of the control groups gavaged with water(P < 0.05), but the changes of respiration and rectal temperature had no significant difference(P > 0.05).In the differenthigh temperature heat-stressed condition, the body weight, thymus weight and spleen weight of the heat-stressed rats all decreased in both supplementation of L-Arginine and no supplementation of L-Arginine groups, but these decresses were less in rats with supplementation of L-Arginine groups than those with no supplementation of L-Arginine groups.And the thymus weight and spleen weight of heat stress groups were temperature and time of heat stress dependent.The respiration and rectal temperature followed the increase of temperatare were accelerated and elevated within limits, but there was no significant difference between the groups supplemented with L-Arginine and group without supplemented with L-Arginine(P > 0.05).   Conclusion   Supplied suitable dose of L-Arginine can induce the increase of the body weight, thymus index and spleen index in normal temperature and relieve the atrophy of the thymus and spleen in heat stress.
2004, 20(5): 598-599.
Abstract(719) HTML (209) PDF 310KB(77)
Abstract:
Construction and expression of recombinant expression vector of SARS virus E2 protein
ZHAO Hong-li, SONG Ceng-hui, NIE Jing,
2004, 20(5): 599-600.
Abstract(846) HTML (260) PDF 208KB(72)
Abstract:
  Objective   To construct expression vector and expression in E.coli of SARS virus E2 protein.   Methods   the E2 gene was inserted into pBV220 vector, the recombinant vector of E2 was identificated by restriction endouclease digestion and PCR.E2 protein was expressed in E.coli via induction in 42℃.   Results   the analysis for recombinant vector digested by EcoR I and Pst I and PCR demonstrated that the E2 gene was inserted into pBV220.SDS-PAGE showed that the relative molecular mass(Mr)of the expressed E2 protein was about 16600.   Conclusion   Expression vector of E2 protein was constructed successfully and expressed effectively in E.coli.these results provided an essential preparation for obtaining a larger quantity of recombinant E2 protein for further study.
2004, 20(5): 601-602.
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2004, 20(5): 602-602.
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2004, 20(5): 603-603.
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2004, 20(5): 604-604.
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2004, 20(5): 605-605.
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2004, 20(5): 606-607.
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2004, 20(5): 607-607.
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2004, 20(5): 608-608.
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2004, 20(5): 609-609.
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2004, 20(5): 610-610.
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2004, 20(5): 611-612.
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2004, 20(5): 612-612.
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2004, 20(5): 613-614.
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2004, 20(5): 614-614.
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2004, 20(5): 615-616.
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2004, 20(5): 616-616.
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2004, 20(5): 617-617.
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2004, 20(5): 618-618.
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2004, 20(5): 619-620.
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2004, 20(5): 621-623.
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2004, 20(5): 623-623.
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2004, 20(5): 624-625.
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2004, 20(5): 626-627.
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2004, 20(5): 627-627.
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2004, 20(5): 628-629.
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2004, 20(5): 630-630.
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2004, 20(5): 631-631.
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2004, 20(5): 632-633.
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2004, 20(5): 634-634.
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2004, 20(5): 635-635.
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2004, 20(5): 636-637.
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2004, 20(5): 637-637.
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2004, 20(5): 638-640.
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2004, 20(5): 640-640.
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