Advance Search

2006 Vol. 22, No. 9

Display Method:
Investigation on paternal-fetal transmission of hepatitis B virus in neonates
ZHOU Xiaohui, WANG Ying, YU Guiqin,
2006, 22(9): 1025-1026. doi: 10.11847/zgggws2006-22-09-01
Abstract(995) HTML (317) PDF 372KB(50)
Abstract:
  Objective   To evaluate the infection and prevalence of hepatitis B virus(HBV)in the neonates whose fathers were chronic HBV carriers.   Methods   230 neonates whose fathers were chronic HBV carriers and mothers were negative for any HBV marker were chosen to detect HBV marker(HBVM), 40 neonates whose parents were both negative for any HBV marker were controlled.   Results   The serum HBV DNA of 44 neonates in studied group(19.13%)were detected positive from their umbilical blood whereas those in controlled group had negative for HBVM.The serum HBV DNA were 6.851±2.317(logarithm)in the neonates and 7.345±1.572(logarithm)in their fathers.There was not correlation between the neonates and their fathers in serum HBV DNA levels.   Conclusion   The HBV infectious rates among the neonates whose fathers are HBV carriers are relatively higher.The hepatitis B virus can be transmitted from the fathers to their neonates vertically.The level of serum HBV replication of fathers with chronic HBV carrier is no correlation with their neonates.
2006, 22(9): 1026-1027. doi: 10.11847/zgggws2006-22-09-02
Abstract(891) HTML (202) PDF 193KB(37)
Abstract:
Investigation on RTI/sex related knowledge, attitude and performance in senior high school students
LIANG Xiu, YU Li, ZENG Guang,
2006, 22(9): 1028-1029. doi: 10.11847/zgggws2006-22-09-03
Abstract(1309) HTML (266) PDF 190KB(32)
Abstract:
  Objective   To find out the situation of the senicr high school students in Shenzhen city about RTIs/sex related knowledge, att itude, performance(KAP), asking for help and seeing a doctor.And to discuss the solution.   Methods   The liberal and vocational senior high school students in Shenzhen city were chosen as visitors and investig ators and the sociological visiting and epidemio logical questionnaire investigating methods were used.   Results   The awareness rate about AIDS knowledge was 50%, The awareness rate about RTI symptom was 0, The rate of giving correct answer about condom knowledge was 64.55%, the rate of using condoms was low.Their attitude to puppy love and sex behavior before marriage were acceptable and even encour aging.Most of them saw a doctor in private clinique and asked their friends or classmates for help.   Conclusion   The sex concept and sex behavior of the high school students trend to more open day by day, but their knowledge about sex, sex diseases and preventive knowledge of AIDS are less, their manner of asking for help and seeing a doctor are unsafe.Therefore, AIDS education development in high school should combine with healthy sex educat ion closely, to guide the high school students to ask for help and see a doctor properly will be an important preventive measure.
Clinical characteristics and risk factors of Norwalk-like virus infection in Children
LIU Yi, NIE Jun, ZHAN Huichun,
2006, 22(9): 1030-1031. doi: 10.11847/zgggws2006-22-09-04
Abstract(1382) HTML (254) PDF 349KB(41)
Abstract:
  Objective   To understand the clinical characteristics and risk factors of Norwalk-like virus(NLVs)infection in children with diarrhea.   Methods   Fecal specimens were collected from children with acute gastroenteritis and their parents were inquired with a questionnaire at the same time.The specimens were detected for rotavirus(RV)and NLVs by ELISA and RT-PCR respectively.The questionnaire data was analysed by 1:1 paired case(NLVs)-case(RV)comparision study and 1:1 paired case-contol study for its clinical characteristic and risk factors.   Results   648 fecal specimens were collected and detected.As a result, 339 RV positive specimens and 80 NLVs positive specimens were found.The clinical characteristic between NLVs cases and RV cases was different.Among the earliest symptoms, vomitting in NLVs cases(62.82%)was more often occuring than in RV cases(14.10%).The amount of cases with vomiting in NLVs cases were more than in RV cases, also.But the cases with the symptoms such as fever, the highest temperature, the number of days in fever and diarrhea, transfusion or not and the times to see a doctor were more commonly occurring in RV cases than that in NLVs cases.The probable risk factors of NLVs infection were analyzed by one-variable and mutivariable logistic regression and four risk factors were determined in the end, such as eating crude or cold foods, touching with diarrhea cases, having the habit of sucking hands and less washing hands.   Conclusion   The clinical characteristic between NLVs cases and RV cases is different.The symptoms of RV cases are more severe than those of NLVs cases, but the burden brought by NLVs infection cannot be neglected.
Analysis on situation of obesity and its influence factors in children
LIU Junxiang, HUANG Zongneng, SU Hongwei
2006, 22(9): 1032-1033. doi: 10.11847/zgggws2006-22-09-05
Abstract(1704) HTML (312) PDF 206KB(187)
Abstract:
  Objective   To know the situation of Children's obesity in Chongqing region and its dangerous factors, and to provide implementary intervention basis for parents, educational system implementintervention, and prevent relevant diseases in the manhood.   Methods   1 867 Children were sampled from 3~16 years at random in this region, data were collected by questionnaires, Body mass index(BMI)method was used as obesity's diagnosis standard, Children were divided into obesity group and normal contrast group by BMI≥28.0 kg/m2 recommended in China.   Results   Children's obesity rate 12.59% (male 13.83%, female 9.74%).Influence factons analysis rev ealed family's history of obesity; amount of daily carnivorous food intaking; the addition of nutr itie food; time of breast milk feeding and time of the addition of solid food and watching TV were the main influence factors.VC was protection factor.   Conclusion   Children's obesity rate of Chongqing region is obviously hig her than that of previous years level, and displays a developing, early implementary intervenging measures have important meaings to prevent adolescent and manhood obesity and other relevant diseases at this stage.
2006, 22(9): 1033-1034. doi: 10.11847/zgggws2006-22-09-06
Abstract(825) HTML (220) PDF 191KB(29)
Abstract:
Association of calcium sensing receptor gene A986S polymorphism with bone mineral density in Chinese premenarche girls
XIAO Xincai, SU Yixiang, LUO Xiaolin
2006, 22(9): 1035-1037. doi: 10.11847/zgggws2006-22-09-07
Abstract(911) HTML (213) PDF 278KB(36)
Abstract:
  Objective   To assess the proportion of genotypes of CASRA 986S gene polymorphism, and to investigate if the polymorphism of CASRA986S gene is associated with bone mineral density(BMD)in premenarehe Chinese girls.   Methods   152 healthy premenarche girls of Han ethic(9~11.5y)were recruited.BMD and BM Cat the lumber spine(L1-L4), proximal femur, and total body were measured by DXA; Allele-specific PCR was used to amplify A986Spolymorphisms of the CASR gene.   Results   The proportions of genotype AA, AS and SS of CASRA 986S gene were 91.44% 7.89% and 0.67%, respectively.The values of bone mass and bone marker in the subjects with the Sallele decreased by 5% (TBBMD), 10% (TBBMC) and 4.6% (L1-L4L SBMD)and 3.5% in Ostocalcin as compared with those without the Sallele(P < 0.01, respectively).The differences in BMD and BM Cbetween subjects with and without the Sallele remained(P < 0.01)even including the terms of age, height, weight, calcium intake, fat mass, lean mass and physical activity in the general liner model.   Conclusion   Significant association exists between a common polymorphism and bone mineral density in prelnenarehe Chinese girls.The Sallele of A986S polymorphism is also a potential predisposing factor of low bone mineral density.
Relationship between some family factors and accident-proneness among school-aged children
JIN Huiqing, YU Wansheng, LI Yingchu,
2006, 22(9): 1037-1038. doi: 10.11847/zgggws2006-22-09-08
Abstract(1261) HTML (263) PDF 215KB(36)
Abstract:
  Objective   To explore the relationship between some family factors and acciden-tpro neness among school-aged children, and appro ach measures for unintentional injury prevention.   Methods   Factors related to family were collected by structured questionnaire both for acciden-tprone school-aged children and their controls, χ2 Test and unconditional Logistic regression analysis were performed to demonstrate the relationship between these family factors and acciden-tproneness.   Results   There were significances between cases and controls in parents.education and occupation, family structure and scale, guardians and their health state, superv ision behaviors.Multivariate Logistic regression analysis showed that loweducation for father, without parents.supervision, abuse and violence were risk factors of acciden-tproneness for school-aged children.   Conclusion   It is important to elevate education level for rural population and teach them right method to treat children.Farmers should not leave their children to larg-escale families, or to those guardians who are nothealth enough or prefer to punish children, when they move to urban.Additionally, schools and society should give more loving and care to the children from special families such as single-parent family.
2006, 22(9): 1039-1040. doi: 10.11847/zgggws2006-22-09-09
Abstract(777) HTML (206) PDF 209KB(39)
Abstract:
Analysis on heritabilities of physical growth items in child twins
LI Yuling, JI Chengye, LU Shunhua,
2006, 22(9): 1040-1041. doi: 10.11847/zgggws2006-22-09-10
Abstract(1391) HTML (311) PDF 190KB(59)
Abstract:
  Objective   To analyze the heritabilities of physical growth items anditsr elated factors.   Methods   9 development items of 218 twin pairs aged 6 to 13 were investigated.The heritabilities were estimatded using intraclass correlation coefficient from the adjusted data for age.   Results   Most of the estimated her itabilities of these items were above 60%, and with sex difference to some extent.   Conclusion   Child development is mainly determined by the genetic factors.The effect of gender on her itabilities is more obvious for weight, head length and head breadth than other items.
2006, 22(9): 1042-1043. doi: 10.11847/zgggws2006-22-09-11
Abstract(853) HTML (226) PDF 201KB(33)
Abstract:
Analysis on drowning among children in rural areas of Guangxi province
NONG Quanxing, YANG Li
2006, 22(9): 1043-1044. doi: 10.11847/zgggws2006-22-09-12
Abstract(1387) HTML (333) PDF 120KB(46)
Abstract:
  Objective   To find out the distributive characteristics and the influencing factors of drowning among children aged during 1 and 14 in rural areas and to provide basis for the prevention and reduction of such incident.   Methods   Selecting 20 towns were selected as a cluster sampling study.133 drow ning children were screened out aged 1~14 from JAN 1.2002 to DEC 31.2004.Astudy on the epidemiological feature of these drowning was conducted.   Results   Boys was higher than girls.The majority occurred during the warmer months: summer and autumn.The places where drowning occurred was mainly at pond and river, Fall into water took the leading type of rural children drowning.Incidence site was the dominating site among these cases.The drowning prevention about safety water was short of.   Conclusion   Intervent ion measures should be carried out to modify the influence factors of drow ning.
2006, 22(9): 1045-1046. doi: 10.11847/zgggws2006-22-09-13
Abstract(810) HTML (167) PDF 179KB(44)
Abstract:
2006, 22(9): 1047-1048. doi: 10.11847/zgggws2006-22-09-14
Abstract(732) HTML (192) PDF 206KB(26)
Abstract:
Epidemiology survey on virological diarrhea among children in rural of Qinhuangdao city
YE qing, TANG Jingyu, HU Haikuan,
2006, 22(9): 1048-1049. doi: 10.11847/zgggws2006-22-09-15
Abstract(1162) HTML (231) PDF 351KB(44)
Abstract:
  Objective   To investigate the epidemiological characterstus of rotanvirus and caliciviruses among children under 5 years of age with acute diarrhea.   Methods   Two viruses were detected by enzyme linked immunosorbent assay [ELISA]and reverse transcription-polymerase chain reaction(RT-PCR), some caliciviruses were cloned and sequenced.   Results   1 236 fecal specimens were detected for rotavirus, 46% was positive and 393 rotavirus negative which fecal specimens were detected for caliciviruses 31.6% was positive.The distribution of month-old The highest was 12 month group for rate of Two virus were infected.followed rotavirus was 9 month group(26.1%)6 month group(14.4%)and calicivirus was 6 month group(25.6%), 9~month group(17.9%).The distribution of seasons two riruses maily were infected in 12 month, 1month and 2 month.Rotavirus Gserotyping results identified G3(45%)to be the most common strain.followed by G1 (35%), G2(8%), G4(3%), G9(0.6%).mixed infections(1%)and 8% of the strains remained untypeable.Caliciviruses.results identified: All strains belonged to NLV GVⅡ in which 6strains GⅡ-3, 2 strains GⅡ-4 and 3 stains GⅡ-7.   Conclusion   Rotavirus is the main cause of diarrhea in chidren and caliciviruses is the next one.
2006, 22(9): 1050-1050.
Abstract(1143) HTML (258) PDF 180KB(59)
Abstract:
Effect of vitamin E on antioxidative activity and DNA damage in rats
SUN Yongye, MA Aiguo, HAN Xiuxia
2006, 22(9): 1051-1052. doi: 10.11847/zgggws2006-22-09-17
Abstract(1052) HTML (220) PDF 327KB(58)
Abstract:
  Objective   To investigate the effect of vitamin E supplementation on antioxidative acivity and DNA oxidative damage in rats.   Methods   42 weaning Wistar rats were randomly divided into 3 groups including control group, S1 and S2 groups.They were fed with fully compounded fodder for 8 weeks.The content of vitamin E in each group diet was separately 24.18, 70.83, 114.04 mg/kg.Whole blood was collected by the end of the trial.The level of plasma VE was analyzed by fluorescene spectrometry.Plasma glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)were analyzed by kits.DNA damage were analyzed by single-cell gel electrophoresis(SCGE).   Results   The levels of plasma VE in S1 and S2 groups were higher than that of the control group.There was no difference of plasma GSH-Px and MDA between the S1, S2 groups and the control group.Intrinsic DNA damage had no differences.Induced by 5, 10 and 25μmol/L H2O2, DNA damage were enhanced while there were still no differences among those groups(P > 0.05).   Conclusion   VE supplementation has no effect on the plasma GSHPx and MDA of healthy young rats and it shows no protective activity on the intrinsic and H2O2 induced DNA damage.
2006, 22(9): 1052-1052.
Abstract(649) HTML (190) PDF 130KB(24)
Abstract:
Effects of high dose VE and VC on cell activities in rats
WANG Qiuzhen, MA Aiguo, SUN Yongye,
2006, 22(9): 1053-1055. doi: 10.11847/zgggws2006-22-09-19
Abstract(1265) HTML (319) PDF 504KB(79)
Abstract:
  Objective   To study the effects of high dose VE and VC on peripheral blood lymphocyte proliferation and anti-DNA oxidative damage activities and erythrocyte membrane fluidity.   Methods   Wistar rats were randomly divided into six groups including control, VC, VE1, VE2, VE1+VC and VE2+VC groups.The trial lasted 8 weeks.Fluorescence polarization method, methyl thiazolyl tetrazolium(MTT)and comet assay were used to detect erythrocyte membrane fluidity, lymphocyte transformation rate and DNA oxidative damage respectively.   Results   In VE1〔33 mg/(kgºd)〕group, plasma MDA was lowered while erythrocyte membrane glutathione peruxidase(GSHPx)activity increased significantly.P(fluorescence polarization)and η(microviscosity), which were inversely related with membrane fluidity, were significantly lowered.Compared with the control, lymphocyte transformation rate in VE1 group was significantly increased by 146.54% and DNA oxidative damage induced by 10μmol/1 H2O2 was significantly lowered.There was significant antagonism effect between 1 000 mg/(kgºd) VC and 33 mg/(kgºd)VE and lowered cell function was seen in the combined group.   Conclusion   Relatively high dose VE could effectively enhance lymphocyte proliferation, DNA stability and erythrocyte membrane fluidity.Buthigh dose VC could cause antagonism effect.Too excessive VE and VC showed no favourable effects and whatever more the activities of peripheral blood cell might be decreased.
Effects of excessive Vitamin A, Zn or both intaken on rat spleen
ZHOU Yuanyuan, WANG Yu, MA Jianhua
2006, 22(9): 1055-1056. doi: 10.11847/zgggws2006-22-09-20
Abstract(1188) HTML (238) PDF 203KB(34)
Abstract:
  Objective   To study the effect of excessive VitA, Zn or both intaken on lipid peroxidation and antioxidant status and cell cycle of normal adult rat spleen.   Methods   The rat model was induced by lavaging solution containing VitA, Zn or both.After 2w and 3w, half numbers of every group were killed and measured the contents of total antioxidation pabilit y (T-AOC), the glutathione perox idase(GSH-Px), the malondialdehyde(MDA), the supero xide dismutase(SOD), and the cell cycle of spleen was detected by flowcytometry.   Results   The T-AOC decreased significantly in every test group than the control after 3w.The SODlevel of the united gr oup and Zn group were significantly higher than the control.The expression in cell cycle blocked in the Sand G2/M phase increased significantly in the VitA group and united group.   Conclusion   Ex cessive VitA, Zn or both intaken could lead to a certain extent damage in rat spleen in short term, especially VitAand Zn both intaken.
Effect of vitamin E on antioxidative enzyme and ATPase activity of hepar mitochondria in rats
XU Hongwei, HAN Xiuxia, DU Wei,
2006, 22(9): 1057-1058. doi: 10.11847/zgggws2006-22-09-21
Abstract(967) HTML (250) PDF 217KB(73)
Abstract:
  Objective   To study the effect of vitamin E(VE)on antio xidative enzyme and ATPase activity of hepar mitochondria in rats.   Methods   40 Wistar rats were randomly divided into 4 groups based on body weight, which was control, VE1, VE2, and VE3 group.The control was fed with normal rat diet and the other three inter fered groups were supplemented with different levels of VE enriched diets which contained VE 335, 1 340, 5025 mg/kg, respecively.The perido of the trial was about 10 weeks.At the end of the trial, the mitochondric were extracted from hepars.The activties Na+-K+-ATPase, Ca2+-Mg2+-ATPase; Superoxide dismutase(SOD); Glutathione peroxidase(GSH-Px)and the contents of malo ndialdehyde (MDA)were measured.   Results   Compared with the control group, VE1 group increased the activities of Na+-K+-ATPase, Ca2+-Mg2+-AT Pase, SOD, GSH-Px(P < 0.05), and decr eased the contents of M DA(P < 0.05).Compared with the control group, the activities of antioxidant enzymes and ATPase in the hepar mitochondria of VE2;VE3 group had not much change.Compared with VE1 group, VE2;VE3 group decreased the activit ies of Na+-K+-AT Pase, Ca2+-Mg2+-ATPase, SOD, GSH-Px(P < 0.05), increased the contents of MDA(P < 0.05).   Conclusion   335 mg/kg VE could increase the activities of antioxidant enzymes and ATPase.1 340, 5 025 mg/kg VE could not further incr ease the activities of antiox idant enzymes and ATPase.
Relationship between absorption of dietary calcium and Bsm I polymorphism of VDR gene in childbearing women
HUANG Zhenwu, DONG Jie, PIAO Jianhua,
2006, 22(9): 1058-1059. doi: 10.11847/zgggws2006-22-09-22
Abstract(999) HTML (261) PDF 363KB(34)
Abstract:
  Objective   To examine the relationship between the absorption of dietary calcium and Vitamin D3 receptor (VDR)gene Bsm I RFLPs in childbearing women.   Methods   Forty subjects were selected from three hundred young famale students aged 18~23 in 2001.After identifying the VDR gene FokI RFLPs, the women were given the representative diets during the 3-day adaptation period and the 12-day metaboism period.The copy food and drinking for measurement of untrients and also the complete feces each day were collected.   Results   As expected, the significant difference of the absorption of diet calcium between VDRBsm Igene RFLPs(bb < Bb)in chidbearing women was observed(P < 0.05).   Conclusion   More attention should be paid to the low calcium absorption of chidbearing women with Bb in our country.
Anti-fibrosis effects of vitamins on rats with immune hepatic fibrosis
LI Qing, MA Yuxia, LIU Dianwu,
2006, 22(9): 1060-1062. doi: 10.11847/zgggws2006-22-09-23
Abstract(1108) HTML (233) PDF 472KB(24)
Abstract:
  Objective   To research and to compare the effects of several vitamin on rats with hepatic fibrosis.   Methods   The rats with immune hepatic fibrosis were treated by VA, β-CAR, VC and VE.The anti-fibrosis effects were evaluated and compared with histopathology of liver tissue, four fibrosis serum marker and the expression of TGFβ1 mRNA and TIMR-1 mRNA in liver tissue.   Results   The semiquantitative scoring system(SSS)of liver tissue were 8.00±4.14, 6.25±4.47, 13.13±7.54 and 5.06±3.06 in VAβ, -CAR, VC and VE group respectively.The SSS was significantly lower in VA β, -CAR and VE group compared with model group, but no significantly difference between VC and model group was found.The general scoring of four fibrosis serum markers in VAβ, -CAR and VE group was lower than that in model group.The general scoring in VE group was also lower than that in VA and VC group but no significantly difference berween VE and β-carotene group.The expression of T GFβ1 mRNA in liver tissue was markekly β-carotene group.The expression of TGFβ1 mRMA in liver tissue was markedly inhibited by VAβ, -CAR and VE and the expression of TIMP-1 mRNA in liver tissue was also inhibited by VE.   Conclusion   VAβ, -CAR and VE all have anti-fibrosis effects on rats and VE is more excellent than VA on anti-fibrosis effects but no difference compared withβ-CAR.No effects are found in VC group.
2006, 22(9): 1062-1062. doi: 10.11847/zgggws2006-22-09-24
Abstract(872) HTML (252) PDF 129KB(37)
Abstract:
Association of vitamin D receptor polymorphism with susceptibility to esohageal cancer
HUANG Kefeng, LI Huiqing
2006, 22(9): 1063-1064. doi: 10.11847/zgggws2006-22-09-25
Abstract(812) HTML (214) PDF 319KB(30)
Abstract:
  Objective   To explore the association between vitamin D receptor(VDR)polymorphism and the susceptibility to esophageal cancer.   Methods   Polymerase chain reaction-restriction fragent length polymorphism(PCR2RFLP)method was used.A restriction fragment length polymorphism in the 3'region of VDR gene, TaqⅠ, was tested in 92 patients with esophageal cancer and 115 healthy controls.   Results   Allele frequencies of TaqⅠpolymorphism had significant differences between the two groups.Compared with people with TT genotype, those with Tt and tt genotypes had less risk to esophageal cancer.   Conclusion   There is an association between VDR plymorphism and the susceptibility to esophageal cancer, and Tt and tt genotypes decline the susceptibility to esophageal cancer.
Protective effects of vitamin E on peripheral nerve function of rabbits exposed to lead
HUANG Chenping, LIN Lin, YE Xiaolei,
2006, 22(9): 1064-1065. doi: 10.11847/zgggws2006-22-09-26
Abstract(1133) HTML (235) PDF 209KB(40)
Abstract:
  Objective   To study the effects of Vitamin E(VE)on the lipid peroxidation and Nerve Conduction Velocity (NCV)of rabbits exposed to lead(Pb).   Methods   24 rabbits were randomly divided into 3 groups(Pb group(Ⅰ), Pb+VE group(Ⅱ)and control group).Ⅰ and Ⅱ group rabbits drank water of 0.1% lead acetate freely for 8 weeks, Ⅱgroup rabbits were still given 2 mg/kg VE ih, 2 times a week.Control group rabbits drank no Pb water and had no VE treatment.At the end of the experiment, the thigh NCV, Malondialdehyde(MDA)and Tota-l Superox ide Dismutase(T-SOD)in plasma and brain were mearsued.   Results   The NCV of Ⅰ group were sig nificantly decr eased(P < 0.01)than those of the control andⅡ groups.There were significantly negative correlation between NCV and plasma or brain SOD(r values: -0.408, -0.464, P < 0.05), but the differences of SODor MDA among the groups were still insignificant in statistically.   Conclusion   Lead poisoning can decrease NCV, which has a relationship with the level of lipid perox idation.The effects of lipid perox idation may be the mechanism of peripheral neur opathy induced by lead poisoning.VE can alleviate the har mful effects of lead by its anti-oxidation and impr oving the function of peripher al nerves.
Construction of cell models transfected with HBV adr subtype
ZHAO Yanfang, YAN Yongping, ZHANG Lei,
2006, 22(9): 1066-1068. doi: 10.11847/zgggws2006-22-09-27
Abstract(1135) HTML (279) PDF 640KB(35)
Abstract:
  Objective   To establish HBV replication cell models.   Methods   Using molecular subclone technique,pcDNA3-3 HBV was constructed by insertion of 9 600 bp fragment of HBV adr subtype genome into the EcoRⅠ and HindⅢ sites of pcDNA3.Hep G2 was transfected with pcDNA3-3 HBV by using lipofectamine transfection reagent and screened with antibiotic G418.   Results   The plasmid pcDNA3-3 HBV was constructed successfully.After stable transfection,HBsAg and HBeAg were positive in the supernatant which tested by ELISA.S gene and pre-S gene were also positive which tested by PCR.cccDNA was found in the cells by PCR,and HBV pre2S1 mRNA was measured by RT-PCR.   Conclusion   pcDNA3-3 HBV can be expressed,transcribed and replicated in Hep G2 cells.
Protection effect of metal net-like defense cloth on cell after electromagnetic pulse
JIN Hua, WANG Dewen, PENG Ruiyun,
2006, 22(9): 1068-1069.
Abstract(1194) HTML (261) PDF 345KB(45)
Abstract:
  Objective   To study the protection effect of metal net-like defense cloth in vivo and genistein on J urkat cell after electromanetic pulse(EMP).   Methods   Wistar rats and Jurkat cells were exposed to high power EMP of 6×104 V/m as experimental models.By various methods such as biochemical tests, light microscopy, methyl thiazolyl tetrazolium(MTT), flow cutometry, the protection effect of metal net-like defense cloth and protein tyrosine kinase(PTK)inhibitor Genistein on immune organization and cell were investigsted.   Results   (1) Peripheral blood lymphocyte of rats sheltered with metal net-like defense cloth(EM-4)recovered, more quickly than that of rats unsheltered; (2)Pretreatment with gensitein could partially block the degression of EMP on the proliferation activity and Jirkat cell in S phsase instantly.   Conclusion   (EM-4) and PTK inhibitor Genistein are effective to protect the immune function, cell proliferation activity and DNA synthesis from EMP radiation.
Relationship between deletion of GSTM1, GSTT1 genes and susceptibility to lung cancer
YAO Wu, WANG Na, WU Yongjun,
2006, 22(9): 1070-1072. doi: 10.11847/zgggws2006-22-09-29
Abstract(3512) HTML (449) PDF 536KB(80)
Abstract:
  Objective   To study the relationship between the deletion of glutathione S transferase M1(GSTM1), glutathione S transferase T1(GSTT1)and susceptibility to lung cancer and its mechanism.   Methods   Using the method of PCR to detect the genotypes of GSTM1 and GSTT1 in 77 lung cancer patients and 107 healthy people.Using SDS-PAGE and Western-blot to detect the expression of P16 protein in normal lung tissue.   Results   There were 58.4% (45/77)patients with GSTM1 null genotype in all cases and 42.1% (45/107)persons with GSTM1 null genotype in the healthy people, and there was significant difference between the two groups(χ2=4.811, P=0.028), and its OR was 1.938(95% CI=1.095~3.581);There were 57.1% (44/77)patients with GSTT1 null genotype in all cases and 50.5% (54/107)persons with GSTT1 null genotype in the healthy people, and there wasn't significant difference between the two groups(χ2=0.802, P= 0.371);There were 26 cases with GSTM1 null/GSTT1 null genotype in patients which was significantly higher than those in the healthy people, and its OR was 2.451(95% CI=1.067~5.633).There was significant difference on the levels of P16 protein between individuals with GSTM1 null genotype and those with GSTM1 non-null genotype as well as the group of GSTM1/GSTT1 combined null genotype and those of GSTM1/GSTT1 combined positive(P < 0.05).   Conclusion   GSTM1 null genotype and GSTM1/GSTT1 combined null genotype laid in much more lung cancer patients than healthy people, and it could highly increase the risk of lung cancer occurrence.The deletion of GSTM1 gene and GSTM1/GSTT1 combined null genotype were related to the low expression of P16 protein.
Inhibition of sulfur dioxide on cytochrome P4501 A in rats
QIN Guohua, MENG Ziqiang
2006, 22(9): 1073-1074. doi: 10.11847/zgggws2006-22-09-30
Abstract(902) HTML (221) PDF 376KB(42)
Abstract:
  Objective   To study the effects of sulfur dioxide(SO2)on hepatic and pulmonary cytochrome P4501A1 (CYP1A1)and 1A2(CYP1A2)in rats.   Methods   Male Wistar rats were housed in exposure chambers and treated with different concentrations of SO2.Fluorescence spectrophotometry was used to study the activities of CYP1A1 and 1A2.The mRNA levels of CYP1A1 and 1A2 were analyzed in livers and lungs by using a real-time reverse transcription-polymerase chain reaction(real-time RT2PCR)assay.   Results   The mRNA levels of CYP1A1 and 1A2 in lungs and livers, the activities of CYP1A1 in livers and those of CYP1A1 and CYP1A2 in lungs were significantly decreased in rats exposed to SO2 at 14 and 28 mg/m3.Furthermore, the decreases of activities and mRNA levels of these P450 enzymes caused by SO2 at different concentrations in lungs and livers of rats followed linear dose-response curves.The hepatic CYP1A2 activities were decrased in rats exposed to SO2 at 56 mg/m3.   Conclusion   SO2 exposure can suppress the activities and mRNA levels of CYP1A1 and 1A2 in the lungs and livers of rats.The capacity of the liver and the lung to metabolize drugs and xenobiotics can be compromised in both animals and humans.
Relationship between endemic water exposure and prevalence of schistosomiasis in mountainous type
ZHONG Bo, ZHANG Yi, LAI Yuhua,
2006, 22(9): 1075-1077. doi: 10.11847/zgggws2006-22-09-31
Abstract(1032) HTML (246) PDF 245KB(38)
Abstract:
  Objective   To study dos-eresponse relationship forendemic waterexposure and prevalence of schistosomiasis of mountain type, in order to evaluate the advantages and disadvantages and applicability of the 3 indexes of endemic water exposure.   Methods   One thousand and thirty three residents of the target population who were living around Qiong Sea were selected at random in November 2001.Three slices of Kato-Katz and 3 times Hatching for 3 stool samples to each residence were used to diagnosis schistosomiasis infection.The frequency of endemic waterex posure(frequency), the average time of endemic waterexposure(Index A)and the average density of endemic waterexposure(Index B)during April to October 2001 of the target population were studied by review questionnaire.   Results   The dos-eresponse relationship was existed among three endemic waterexposure and schistosomiasis prevalence.In formerly study, the typical dose-response relationship was existed (χ2=17.76, P < 0.01)between frequency and prevalence in the natural village with schistosomiasis prevalence > 10%;theidentical dose-response relationship was existed(χ2=11.08, P < 0.01)between Index A and prevalence in the natural village with schistosomiasis prevalence < 10%.The dose-response relationship was not clear when the relation among indexes of endemic waterexposure and schistosomiasis prevalence was fluctuation under other condition.   Conclusion   In the mountainous epidemic type of schistosomiasis, the frequency can be used for the endemic water exposure evaluation of the population with higher prevalence, index A can be used for the endemic water exposure evaluation of the population with lower prevalence.
2006, 22(9): 1077-1077. doi: 10.11847/zgggws2006-22-09-32
Abstract(823) HTML (239) PDF 114KB(33)
Abstract:
Association between manganese superoxide dismutase(MnSOD)9 Ala/Val genetic polymorphisms and coronary artery disease
CHI Dongsheng, LING Wenhua, MA Jing,
2006, 22(9): 1078-1080. doi: 10.11847/zgggws2006-22-09-33
Abstract(893) HTML (193) PDF 335KB(91)
Abstract:
  Objective   To study the associations between manganese super oxide dismutase 9 Ala~Val genetic polymorphisms(MnSOD9 Ala/Val)and coronary artery disease(CAD), plasma activities of total superoxide dismutase(T-SOD), Mn-SOD, as well as plasma concentration of maleic dialdehyde(MDA).   Methods   Using PCR-RFLP method to identify genotype of MnSOD9 Ala/Val genetic polymor phisms, and using colorimetry to detect plasma activities of T-SOD, MnSOD and plasma concentration of MDA in 262 CAD patients and 100 controls.   Results   Compared with controls, the plasma act ivities of TSOD and MnSOD reduced obviously[(23.61±16.51)U/ml vs(44.01±22.68)U/ml and(21.56±13.11)U/ml vs(28.79 ±8.65)U/ml, P < 0.001 andP < 0.001, respectively), while plasma MDAco ncentration in creased markedly[(2.47± 0.73)nmol/ml vs(2.15±0.55)nmol/ml, P < 0.01).There were more AA genoty pe and A allele of MnSOD 9 Ala/Val genetic polymorphisms(64.2% vs 43.0%, P < 0.001 and 80.0% vs 67.0%, P=0.014, respectively)than controls in CAD patients.The activ ity of plasma MnSOD depressed in individuals with MnSOD AA genotype compared with those with VV genotype(22.87±13.47 vs 32.04±9.19 U/ml, P < 0.01).MnSOD correlated negatively with MDA(r=-0.15, P < 0.01).   Conclusion   The plasma activities of T-SOD and MnSOD reduce obviously, in contrary, plasma concentration of MDA increases mar kedly in CADpat ients.The activity of plasma MnSODdepress in individuals with MnSOD AAgeno type.
Inhibition of High effect cypermethrin on Ca-ATPase of Blattella germanica
MA Yi, MA Min, ZHOU Tianhong,
2006, 22(9): 1080-1081. doi: 10.11847/zgggws2006-22-09-34
Abstract(1112) HTML (322) PDF 212KB(24)
Abstract:
  Objective   To more perfectly undestand the operational mechanisms of high effect cyper methrin by researching the dosage effect and the time effect between high effect cypermethr in and Ca-ATPase activities of Blattella germanica.   Methods   Quantitative high effect cypermethr in of different concentration was sprayed upon the method of GB 13917.1-13917.8-92 for insecticide registered testing indoo reffect.The activities of Ca-ATPase were determined by using ATPase Kit.   Results   Inhibition of high effect cyper methrin on Ca-ATPase activities of Blattella germanica showed concentration gradient effectin 48 hours.The results showed the correlation of concentration of high effect cypermethr in and inhibiting time effect on Ca-ATPase activities of Blattella germanica in 144 hours and death rate of Blattella germanica in 72 hours was positive relationship.   Conclusion   Ca-ATPase is one of the targets of high effect cypermethrin.Because of sensitivity decrease of Na-K-ATPase, tar get resistance may be one of the important resistant mechanisms to high effect cypermethrin.
Micronuclei and DNA damage in peripheral lymphocyte of workers occupationally exposed to octachlorodipropyl ether
TANG Meng, YE Mingxian, YUAN Zhenhua,
2006, 22(9): 1082-1083. doi: 10.11847/zgggws2006-22-09-35
Abstract(1487) HTML (294) PDF 190KB(38)
Abstract:
  Objective   To understand the effects of octachlorodipropyl ether on the health of workers.   Methods   To make an epidemiological survey of the wor kers occupationally exposed to octachlorodipropyl ether(S2)by micronucleus test and single cell gel electrophoresis assay(SCG E).   Results   There was no significant increase in frequency of micronuclei of those-exposed to octachlorodipropyl ether(S2)as compared with the control group.DNA damage was more severe in the group exposed to octachlorodipropyl ether(S2).   Conclusion   Occupational exposure to S2 producing process may cause DNAdamages in peripheral Lymphocyte of workers.
Roles of heat shock protein 70 family in antigen presentation
HUANG Weiyan, YU Quan, ZHAO Mei
2006, 22(9): 1084-1085. doi: 10.11847/zgggws2006-22-09-36
Abstract(1027) HTML (291) PDF 294KB(32)
Abstract:
  Objective   To study the ability o f Heat shock protein 70 family in the procession of the antigen presentation and discuss the relationship of Hsp70 family and the tumor immunogenesis.   Methods   Antisense hsp70, ant isense hsc70 and B7 express plasmid were transfected into tumor cell line B16, and the expression of heat shock protein 70 and the effect of inhibiting Hsp on Tlymphocyte proliferation were detected after 24 hours.   Results   The expression of heat shock protein 70 were inhibited in B16 after transfection of pLXSN-antihsp70, pLXSN-antihsc70, the efficiency of T cell proliferation was declined.In the B16 cell with pLXSNmB7, hsp70 expression were promoted and the efficiency of T cell prolifer ation was increased.   Conclusion   Inhibiting the expression of Hsp70 family can induce the decline of the efficiency of T cell proliferation.Transfect ing pLXSNmB7 into tumor cell can improve the expression of Hsp70 family and the ability of antigen presentation.
Epidemic analysis of Cionorchis sinensis infection in Dalian city
CUI Yu, ZHEN Lili, CHEN Fengyi,
2006, 22(9): 1086-1087. doi: 10.11847/zgggws2006-22-09-37
Abstract(1015) HTML (322) PDF 369KB(33)
Abstract:
  Objective   To analysis breeding ground of Clonorchis sinensis and explore the epidemiology basis lead to Clonorchis sinensis.   Methods   Through investigating fresh-water fishes infected by liver fluke worms in Fuzhou town to Dalian area, its epidemiology was analysed.   Results   Metacer caria had existed in the gills and muscular tissue of the fish from the stream flow of Fuzhou town and the fishponds of farmers.After inoculation for 2 months, some eggs from catcs feces were found, at the same time, some adults from catcs hepatic ducts and bile ducts were obtained.According to identifying of etiology, these adults were confirmed to Clonorchis sinensis.   Conclusion   Fuzhou town existed breeding ground of Clonorchis sinensis.Improving eating habits and strengthening health propaganda were significant means of controling the infection and dissemination of clono rchiasis.
Analysis on musculature proteome of rat fetus by two-dimensional gel electrophoresis
ZHOU Haitao, FU Weineng, JIN Chunlian,
2006, 22(9): 1087-1088. doi: 10.11847/zgggws2006-22-09-38
Abstract(1094) HTML (249) PDF 315KB(19)
Abstract:
  Objective   To establish protocol of two-dimensional Gel Electrophor esis for analysing the proteome of mus-culature of Wistar rat fetus.   Methods   Proteins extracted from tibia-fibulae musculature isolated from 21-day ratfetus were loaded on immobile pH gradient gels to process the first dimension isoelectric focusing electro phoresis and then transfer to SDS poly acrlamide gel for the second dimension electrophoresis.Following the electrophoresis Coomassie Brilliant Blue staining or silver staining was performed, and the images were scanned and analyzed with Image Master 5.0 software package.One set or protein spots matching from three separate gels were cut for MALDI-TOF mass spectrometry analysis.   Results   Images with good reproducibility of the proteome of musculature were obtained after tow dimensional electrophoresis.The reproducibility of 2D gelelectr ophoresis was verified through comparing of peptide mass fingerprints form matching protein spots of three separate gels which sug gested a same protein(light chain 1 of skeletal muscle myotonin).   Conclusion   The utilization of 2D electrophoresis can satisfactorily separ ate muscular proteins from ratembryos and achieve a high resolution and good repr oducibility for protein identification.
Distribution of non-iodized salt and intervention effect in areas with iodine deficiency in Shandong province
GUO Xiaowei, QIN Qiliang, LIU Chuanjiao,
2006, 22(9): 1089-1090. doi: 10.11847/zgggws2006-22-09-39
Abstract(1207) HTML (277) PDF 203KB(61)
Abstract:
  Objective   To investigate update distribution of non-iodized salt in iodine deficiency areas so as to assess the effect of controlling it in Shandong province.   Methods   Edible salts were sampled and tested at the levels of producers and households by cross-section survey.Palpation of the thyroid of school-age children was carried out, their urinary iodine level was detected by random sampling.The indicators were compared with previous ones.   Results   The rate of iodized salts from factories was 94.6% with mean value(32.7±6.4)mg/kg.Only in 1999 the rate of it was below 90%.27, 423 samples of edible salts from households were tested.The proportion of iodized salt was 95.5%, qualification iodized salt 91.2% and non-iodized salt 4.5%.The ratio of iodized salt was 5.9%, qualified iodized 91.1% and non-iodized salt 4.1% in whole province.The proportions of iodized salt in 87.6% of counties were over 90%, 74.2% in qualification rate(≥90%)and 12.4% in non-iodized salt rate(> 10%).75.0% of counties with non-iodized salt were at coastal regions, 25% were at inland.The number of the counties was lower in 2002 or 2003 than in 1999, but 2004 was more then before.Goiter prevalence in school-age children was 7.7%.Their urinary iodine median was 199.1 μg/L.   Conclusion   The rates of iodized salt and qualification iodized salt have reached the tar get of IDD elimination at the levels of province.It should strengthen synthesizing intervention activit ies against non-iodized salt in the same costal counties for the goal.
Homology analysis of resistant plasmids in nosocomial infection Escherichia coli
SHI Fuyan, BAI Yana, FAN Jingchun,
2006, 22(9): 1091-1092. doi: 10.11847/zgggws2006-22-09-40
Abstract(921) HTML (261) PDF 334KB(28)
Abstract:
  Objective   To understand the epidemic condition about the resistant plasmids and their homology of the E.coli obtained from two hospitals in Gansu province.   Methods   The antibiotic sensitivity test, plasmid elimination, plasmidpro-file, restriction endonuclease profile were used in analyzing tho E.coli resistant plasmids.   Results   25 strains of E.coli were isolated from 196 samples in two different hospitals, the isolation r ate was 12.8%.76.0% isolates were resistant to the eight common antibiotics in different degree.Only 28.0% strains were just resistant to one antibiotics, the multi-rcsistant strains amount for 32.0%.There were 19 isolates car ried plasmids(76.0%).The results of plasmid elimination showed 12 strains carried resistant plasmid, and the one with 24.8kb was popular plasmid.The endnuclease profiles revealed that the same source strains had certain homology, there were similiarendonuclease profiles among strains in different wards.   Conclusion   The popular plasmids in different wards had certain homology, and they can be transferred among different strains of bacteria.
A case-control study on influencing factors of asthma in adults
ZHOU Xiaobin, JI Xia, GE Yunjie,
2006, 22(9): 1093-1094. doi: 10.11847/zgggws2006-22-09-41
Abstract(1535) HTML (332) PDF 206KB(62)
Abstract:
  Objective   To explore the influencing factors for ast hma in adults.   Methods   Ahospital-based case-control study was undertaken in 116 asthma patients with 158 controls without asthma by using questionnaire.Available data were analysed by Uni-and Multi-unconditional Logistic regression methods.   Results   12 factors were identified to be related to asthma when single factor Logistic regression analysis.8 factors in muitiple Logistic regression mo del were: the history of dustallergy(OR=9.38), using eider down quilt at home(OR=7.66), the history of food allergy(OR=4.03), the history of allergic rhinitis(OR=3.76), often suppression and depression(OR=3.33), occupational dust and gas expourse(OR=2.47), father smoking(OR=1.49)and breastfeedign(OR=0.29).   Conclusion   Adult asthma was a disease to be associated with muitiple-factors such as lifetyle, environment and gene.The history of dust allergy, using eider down quilt at home, the history of food allergy, the history of allergic rhinitis, often suppression and depression, occupational dust and gas expourse, father smoking increased risk of asthma and breastfeeding decr eased risk of asthma in adults.
Eytotoxicity of volatile organic compounds on Chinese hamster lung cells
XIAO Yun, CHEN Junqiang, ZHOU Huashi,
2006, 22(9): 1095-1096. doi: 10.11847/zgggws2006-22-09-42
Abstract(1146) HTML (295) PDF 125KB(62)
Abstract:
  Objective   To evaluate the cytotoxicity of volatile organic compounds i.e.formaldehyde and benzene on Chinese hamster lung(CHL)cells in vitro.   Methods   CHL cells were exposed to formaldehyde and benzene at different concetration respectively and simultaneously.Cell growth was tested by methylthiazoletrazolium(MTT)assay and absorbency was determined to calculated the cell relative growth rate.   Results   According to MTT assay,the IC50(48 h)of benzene for inhibiting CHL cell proliferation was 48.35μg/ml and the IC50 (48 h)of formaldehyde was 75.10μg/ml.The results showed that a synergistic effect existed between the two chemicals.   Conclusion   Formaldehyde and benzene have a direct cytotoxicity on CHL cells.The toxicity exposed to formaldehyde and benzene simultaneously is greater than that of exposed to formaldehyde and benzene respectively.
Application and assessment of bar code technology in Guangzhou-biobank in cohort study
ZHU Tong, LI Chengyi, JIANG Chaoqiang,
2006, 22(9): 1097-1098. doi: 10.11847/zgggws2006-22-09-43
Abstract(867) HTML (233) PDF 454KB(48)
Abstract:
  Objective   To establish a large biological sample bank from a great number of participants for genetic cohort study.An accurate quick identification must be ensured for each biological sample in the operation, which helped to make it viable for the long term follow-up in the biobank.   Methods   The bar code-identifying technology was applied in the conduct of all procedures including sample collection, handling, storage and retrieval.   Results   A new operating pattern using automatic bar-coding as the principal technology was developed.The biological sample bank including 10 thousand individuals was established.The implementation of the bar code-identifying information system in the huge biobank reduced the handling time for every sample and work efficiency was increased 1.5 times.   Conclusion   Using bar code technology can efficiently avoid errors in distinguishing samples.The developed operating pattern worked accurately, dependably and efficiently in our laboratory.It helps us to achieve computerization completely in medical research and improve the quality of genetic cohort study.
Study on characteristics of arsenic metabolism and DNA damage between mother and child under arsenic exposure
LI Xin, XU Yuanyuan, LI Bing,
2006, 22(9): 1099-1100. doi: 10.11847/zgggws2006-22-09-44
Abstract(816) HTML (189) PDF 327KB(53)
Abstract:
  Objective   To explore the characteristics of arsenic metabolism and DNA damage of children under arsenic exposure through the detection of arsenicals and 8-OHdG in urine, and to explore whether there is a difference of arsenic exposed effects between children and adults.   Methods   Arsenicals were measured by the hydride generation-cold iron captureatomic absorption spectrophotometry; 8-OHdG levels were detected by detection kit.   Results   The level of inorganic arsenic (iAs), monomethylarsonate acid(MMA), dimethylarsinic acid(DMA)and total arsenic(tAs)of female and children of the exposed group were significantly higher than those of control's.Between the arsenic exposed mother and child, the level of iAs, DMA, tAs and DMA/tAs of the child were markedly higher than those of their mother's, while MMA/(MMA+DMA)was significantly lower than that of their mother.There was no significant difference of 8-OHdG level between mother and child.   Conclusion   The secondary arsenic methylation of children under arsenic exposure is stronger than that of adults.There is no significant difference of DNA damage between arsenic exposed children and adults.
Distribution of Connexin 37 gene C1019T polymorphism in Han population in Shanxi province
XIE Xuejian, LI Lansun, GUO Wenyi,
2006, 22(9): 1101-1102. doi: 10.11847/zgggws2006-22-09-45
Abstract(1151) HTML (178) PDF 255KB(20)
Abstract:
  Objective   To investigate the distribution of the gap junction protein relat ed to cardiovascular disease Connexin 37(Cx37)gene C1019T polymor phism in the Han population in Shanxi province.   Methods   The polymerase chain reaction-restriction fragment length polymor phism(PCR-RFL P)technique was used to examine Cx37 gene C1019T polymorphism of 116 unrelated healthy persons.χ2 test was used to compare the geno type and allele frequencies of Han population with those of otherethnic populations.   Results   The allele frequencies of Cx37 gene C1019T were as follows: C=68.5%, T=31.5%.The genotype frequencies were as follows: C/C=63.8%, C/T=30.2%, T/T=6.0%;The allele frequencies of the male were as follows: C=76.1%, T=23.9% and the genotype frequencies were as follows: C/C=59.2%, C/T=33.8%, T/T=7.0%;No significant difference was found in the frequencies of Cx37 gene C1019T genotype and alleles between men and women in Chinese Han population in Shannxi province.(P > 0.05).There were significant differences between Chinese Han population and Swedish population(P < 0.05), but no significant difference was detected between Chinese Han population and those in Japan and Taiwan(P > 0.05).   Conclusion   The frequencies of Cx37 gene C1019T genotype and allele in Shannxi Han population are not different from oriental population, but are different from Hesperian population.
Evaluation on chlorination effect and halogenated hydrocarbon production of hospital wastewater treated by membrane bioreactor
WANG Keli, SUN Yingxue, ZHANG Feng,
2006, 22(9): 1103-1104. doi: 10.11847/zgggws2006-22-09-46
Abstract(997) HTML (316) PDF 326KB(32)
Abstract:
  Objective   To study the advantages that membrane bioreactor(MBR)was used in disinfection of hospital waste water.   Methods   Plate count for the tolal bacteria, filter membrane method for the fecal coliform group and headspace gas chromatography for the halogenated hydrocarbon.   Results   The proper dose of sodium hypochlorie, contact time, total halogenated hydrocarbon production after disinfection of influent and effluent were 14.0 mg/L, 60 mm, 26.40μg/L and 1.0 mg/L, 1.5 min, 7.98μg/L respectively.   Conclusion   Higher disinfectant efficiency and less disinfection by-products(DBPs) production were achieved as for disinfection of MBR effluent at lower dose of disinfectant.For better disinfect effect of hospital wastewater, the dose of disinfectant and contact time could not be intensified bindly.
Analysis on genotype of mumps virus strain circulating in Beijing city
CHEN Meng, CHEN Lijuan, ZHANG Tiegang,
2006, 22(9): 1105-1106. doi: 10.11847/zgggws2006-22-09-47
Abstract(1251) HTML (254) PDF 376KB(47)
Abstract:
  Objective   To understand geographic distribution of mumps virus genotypes and variation of circulating mumps virus strains in Beijing.   Methods   SH genes were analyzed.Genetic homology between the Beijing strains and other reference mumps strains were analyzed.   Results   17 strains were all genotype F, and VAC-S79 was genotype A.The circulating viruses showed 5.4% sequence divergence, and 4.8% sequence divergence from Z77.58(Lanzhou); 7.0% from Z77.60 (Shanghai); 14.6%~15.9% from VAC-s79.   Conclusion   Genotype F is the main genotype of circulating strains in Beijing.Although all the circulating strains have genotype F, there are some variations between them.As the rate of variation is tend to increase, it seems necessary to follow up the molecular epidemiology.
2006, 22(9): 1106-1107. doi: 10.11847/zgggws2006-22-09-48
Abstract(914) HTML (236) PDF 201KB(39)
Abstract:
Expressions of genes related to carbohydrate and lipid metabolisms in diabetic rats with zinc supplementation
SUN Zhong, WU Yuntang, ZHANG Wanqi,
2006, 22(9): 1108-1110. doi: 10.11847/zgggws2006-22-09-49
Abstract(1038) HTML (308) PDF 573KB(24)
Abstract:
  Objective   To study the effects of zinc supplementation on related gene expression of liver in diabetic rats.   Methods   cDNA fragments from former research project were cloned, sequenced and BLASTn analyzed.The RT-PCRs were made using the primers designed according to the sequence of cDNA.   Results   The identities between the sequence of Zn-8 and PP2A was 99%.The expression level of DM group(0.5072±0.0574)was obviously lower than those of normal group(1.3303±0.1855, P < 0.05).And The expression level of DM+Zn group(0.7005±0.1563)had some improvement compared with those of DM group(P < 0.05).   Conclusion   Zinc supplementation can improve the expression of PP2A mRNA on liver in diabetic rats.It may be one of the molecular mechanisms that zinc can improve the disorders of glucose and lipids metabolism in diabetic rats.
2006, 22(9): 1110-1110. doi: 10.11847/zgggws2006-22-09-50
Abstract(1064) HTML (328) PDF 282KB(31)
Abstract:
Effect of soybean peptide intake on catabolism of cholesterol in rats
ZHAO Xiujuan, JIA Li, NIU Yucun,
2006, 22(9): 1111-1113. doi: 10.11847/zgggws2006-22-09-51
Abstract(919) HTML (233) PDF 1762KB(63)
Abstract:
  Objective   To investigate the effects of dietary soybean pr otein intake on the concentration of serum lipids in hypercholesterolemic rats and to study the mechanisms of them.   Methods   Following 28 days of inducing the hyperc-holesterolemia model successfully, rats were fed a purified diet containing either casein or soybean protein.After the experimental diets had been fed for 56 days, fasting animals were killed.   Results   Comparing with the rats in casein group, the total cholesterol(TC), trig lyceride(TG)and apolipoprotein B(apoB)in soybean protein group were significantly low(P < 0.05).The concentrations of fecal excretion of bile acids were higher in the rats of soybean protein group(P < 0.05).Whereas, the changes of high density lipoprotein cholesterol, apolipopr otein AI(apoA-Ⅰ)were not significant in these rats.The levels of 3-hydroxy-3-methylglutaryl coenzyme A(HMG-CoA)reductase mRNAand low density lipoprotein receptor mRNA in soybean protein rats were significantly high.   Conclusion   Dietary soybean protein intake may lower TC.Further resear ches are needed for study.
2006, 22(9): 1113-1114. doi: 10.11847/zgggws2006-22-09-52
Abstract(755) HTML (240) PDF 349KB(23)
Abstract:
Cytotoxic effects of ibotenic acid on hippocampal neurons in rats
GUAN Caixuan, SUN Nai, QU Peng,
2006, 22(9): 1115-1116. doi: 10.11847/zgggws2006-22-09-53
Abstract(1159) HTML (262) PDF 499KB(55)
Abstract:
  Objective   To observe Cytotoxic effects of ibotenic acid on hippocampal neurons in rats, and explore toxico-logical mechanism of ibotenic acid(IBO).   Methods   Wistar rats were given IBO, icv, only once.10 days later, the morphological changes of neurons in hippocampus were observed by HE staining and electronmicroscope respectively, amyloid β-protein precursor(APP)gene expression in hippocampus was measured with in situ hybridization, and the product of amyloid β-protein(Aβ)was determined by immunohistochemistry staining.   Results   IBA, icv, induced neuronal degeneration and loss, higher level of APP gene expression, and high product of Aβ in hippocampus in rats.   Conclusion   Upregulation the level of APP gene expression inducing high product of Aβ maybe one of toxicological mechanisms of IBA involved in hippocampal neurons damage in rats.
Effects of mixed pesticides on somatosensory evoked potential and activities of ATPase of brains in rabbits
LIN Li, ZHANG Jing, ZENG Xiaoli,
2006, 22(9): 1117-1118. doi: 10.11847/zgggws2006-22-09-54
Abstract(813) HTML (196) PDF 238KB(32)
Abstract:
  Objective   To explore the effects of mixed pesticides on the Somatosensory evoked potential(SEP)and the activities of ATPase of brains in rabbits.   Methods   Rabbits were divided into 2 mixed pesticide ex posure groups and 1 control group.A group were poisoned by profenofos and fenvalerate, and B group were poisoned by profenofos and methomyl.The SEP were measured in each group before and after poisoning by mixed pesticide, and the activites of Na+-K+-AT Pase and Ca2+-Mg2+-ATPase of the brain was measured in each group after poisoning by mixed pesticide.   Results   Compared with the control group, the potential periods of N1, P2, N2 in high-dose exposure group in Agroup and Bgroup delayed significantly after poisoning by mixed pesticide(P < 0.01).Compared with the control group, the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase decr eased significantly in high-dose exposure groups and middle-dose groups in A group and B group after poisonging by mixed pesticide(P < 0.05, P < 0.01).   Conclusion   The mixed pesticides can harm the functions of central nervous system, which must be related to the decrease of the activities of Na+ K+-ATPase and Ca2+-Mg2+-ATPase in brain.
2006, 22(9): 1119-1119.
Abstract(733) HTML (206) PDF 228KB(26)
Abstract:
2006, 22(9): 1120-1121. doi: 10.11847/zgggws2006-22-09-56
Abstract(778) HTML (157) PDF 442KB(28)
Abstract:
2006, 22(9): 1121-1121. doi: 10.11847/zgggws2006-22-09-57
Abstract(815) HTML (221) PDF 371KB(27)
Abstract:
2006, 22(9): 1122-1123. doi: 10.11847/zgggws2006-22-09-58
Abstract(969) HTML (494) PDF 227KB(17)
Abstract:
Investigation on knowledge, attitude and practice among college students
TANG Weiming, SHI Chao, ZHOU Qin,
2006, 22(9): 1123-1124. doi: 10.11847/zgggws2006-22-09-59
Abstract(1780) HTML (378) PDF 341KB(78)
Abstract:
  Objective   To realize HIV/AIDS related knowledge,attiude and practice(KAP)among college students and provide the strategy for AIDS prevention.   Methods   A self-filled questionnaire on HIV/AIDS was used randomly among students of ten universities in Nanjing.   Results   98.9% of the students once heard the HIV/ADS,86.3% of them know that this disease was caused by virus,which called HIV;but only 3719% students knew that the World Aids Day was the first day of December.And 57.1% students thought that HIV could be infected by mosquito.At the same time 51.9% students said that they would keep a distance from their friends if they had infected HIV/AIDS,and 49.3% students said that would use condom when they had sex with others.   Conclusion   As a particular pary with education,college students'KAP rate on HIV/AIDS is high,and their attiude on HIV/AIDS is correctitude.Their slef-protection awareness is well.
Logistical regression analysis on influence factors of college students' life quality
SHU Jianping, MAO Zongfu, YIN Ping,
2006, 22(9): 1125-1127. doi: 10.11847/zgggws2006-22-09-60
Abstract(1615) HTML (271) PDF 469KB(80)
Abstract:
  Objective   To discuss the primary influence factors and interventions of univeysity students'life quality.   Methods   1171 Students who major in engineering, management or medicine were asked to fill in WHO's Quality of Life (WHOQOL)-100 and the self-made "influential factors questionnaire".Variable were set as whole life quality, general health situation and the 6 fields of life quality.All scores were processed by logistical regression analysis through monovariate and multivariate.   Results   Excepting the field of spiritual support, classificatory calculating accuracy of 6 models in order were 97.1%, 94.9%, 96.2%, 98.6%, 69.6 and 97.7%.There were 15 factors that influence life quality of univeysity students.The top six primary factors were currenthealth situation, interests in one's major, network motivation, monthly expenditure, gender, and distressed of co-relations.Other factors were studying expectations, real achievement, Grades, Majors, unable to act as monitor, suspending one's schooling for certain reasons, getting punishment for some reasons, failure in examination and parents'education degree.   Conclusion   The life quality of college students are influenced by such factors as individual, university, family and society.The interventions should be focused on strengthening physical health, cultivating positive attitudes and improving economical situation.
2006, 22(9): 1127-1127.
Abstract(815) HTML (194) PDF 116KB(17)
Abstract:
Analysis on obesity and its risk factors among inhabitants of Bortala prefecture of Xinjiang autonomous region
WANG Kun, AO Youte, ZHAO Lei,
2006, 22(9): 1128-1130. doi: 10.11847/zgggws2006-22-09-62
Abstract(1414) HTML (256) PDF 266KB(25)
Abstract:
  Objective   To find out the epidemiological characteristics of overweight and obesity in the inhabitant over 30 year-old from Mongolians, Kazakans, Uygurs and Hans in Bortala Prefecture of Xinjiang, and the relation between obesity and cardiov ascular risk factor.   Methods   The epidemiological study was carried out in 3732 Inhabitants over 30 year-old by cluster sampling.   Results   The prevalence rates of overweight and obesity in this region were 36.02% and 27.39% respectively, which were higher in city than in country.There was not a significant difference in total over weight/obesity between male and female; Male.s overw eight was higher than female's; Female's obesity was higher than male's.Risk of suffering from overweight and obesity was highest in Mongolians.po pulation, lowest in Kazakans.Male of Mongolians.and female of Uygurs.obesity were very high.The risk factors of overweight and obesity were age, gender, nationality, vocation, education level, drinking, meat.Male was much more than female in overweight/obesity with cardiovascular risk factor.   Conclusion   The high morbidity of overw eight and obesity is becoming one of the prominent public health problems in the region investigated, especially obesity.Male of obesity in four ethnic group is high risk group of cardiov ascular disease.
Survey on health status among civil aviation pilots
WANG Qing, CHEN Dafang, HUANG Aiqun,
2006, 22(9): 1131-1132. doi: 10.11847/zgggws2006-22-09-63
Abstract(1325) HTML (257) PDF 179KB(71)
Abstract:
  Objective   To study the health condition of an arilines.pilots, and to offer scientific basis for promoting the health condition of pilots and ensuring the safety of flight.   Methods   Clustering the sample cases from the pilots of an airlines, giving annual physical examination to the same group of pilots from 2000 to 2004, making analysis with disease prevalence rates and type of disease.   Results   (1) The general prevalence rate of this group of pilots were 39.5%, 44.2%, 50.3%, 52.4% and 58.5% from 2000 to 2004, there was an obvious increase in each year(P < 0.01).(2)The main diseases of the pilots were for med of hyperlipide mia, bad eyesight, hearing loss, fatty liver and hyper tension.The prevalence rate of hyperlipidemia had an obvious increase in each year(P < 0.01).(3)The prevalence rate of hyper lipide mia, hearing loss, fatty liver and anatomy disease in the age group above 45 were more higher than that of the age group below 45(P < 0.01).   Conclusion   The main diseases threatening the health of pilots are hyperlipide mia, bad eyesight, hearing loss, fatty liver and hypertension.Healthy knowledge and effective measures should be taken for pilots, accordingly prevent disease and improve their health condition.
2006, 22(9): 1133-1134. doi: 10.11847/zgggws2006-22-09-64
Abstract(790) HTML (153) PDF 209KB(29)
Abstract:
Effect of arsenic trioxide on apoptosis and cell cycle of NB4 cell
DI Chunhong, GU Shaohua, TAN Xiaohua,
2006, 22(9): 1134-1136. doi: 10.11847/zgggws2006-22-09-65
Abstract(1092) HTML (219) PDF 308KB(24)
Abstract:
  Objective   To investigate the changes of apoptosis and cell cycle inducecd by arsenic trioxide(As2O3)inleukemia cell line NB4 cell in vitro.   Methods   NB4 cells were treated with As2O3 in different co ncentrations fo 12, 24 and 36h.The cytotoxity of NB4 cell were detectexd by(MTT)assay.The apoptotic cell was determined by Annexin VFITC-PI double staining method and measured by flow cytometry(FCM).Cell cycle was examined by PI fluoresence.   Results   1~8 Lmol/L As2O3 could induce NB4 cell in a time-and-dose dependent manner in vitro.2~8 Lmol/L As2O3 could induce NB4 cell obvious apoptosis.The apoptosis rate of NB4 cells treated by As2O3 for 12 and 24 h sho wed a line relationship with the concention of As2O3, and for 36 and 48 h the NB4 cell mainly showed later apopotsis.Flow cytometry analysis showed that the number of G2/M phase cells increased gradually with raised concentrations treatment of As2O3.   Conclusion   As2O3 may inhibit the proliferation of NB4 cell.The mechanism is probably through blocking the cell cycle of G2/M phase and induced NB4 apoptosis in a time-and-dose dependent manner.
2006, 22(9): 1137-1138. doi: 10.11847/zgggws2006-22-09-66
Abstract(817) HTML (180) PDF 205KB(19)
Abstract:
Inhibition effects of apoptin gene on murine sarcoma 180 in vivo in mice
WANG Xiaojie, WANG Qiuying, ZHAO Hongli,
2006, 22(9): 1138-1139. doi: 10.11847/zgggws2006-22-09-67
Abstract(1121) HTML (255) PDF 316KB(15)
Abstract:
  Objective   To study the inhibition effects of apoptin gene on inducing regression of murine sarcoma 180 (S180)and prolonging the survival term of mice in vivo.   Methods   Mice bearing S180 were used as the model and treated with the apoptin gene.The survival term, weight of the tumor and the apoptosis of tumor cells were detected respectively.   Results   pcDNA A3 plasmid, which contains apoptin gene, could result in regression or complete remission of S180 grown as xenografts in mice and significantly increased their survival long term by intratumoral transfer.Early after intratumoral injection, apoptin mRNA could be detected in significant quantities by RT-PCR.Furthermore, cell death and disruption of the tumor integrity were apparent in the transduced regions.   Conclusion   This exper imental gene therapeutic strategy constitutes a unique example of specific antitumor activiy using apoptin gene.
2006, 22(9): 1140-1141. doi: 10.11847/zgggws2006-22-09-68
Abstract(919) HTML (210) PDF 200KB(39)
Abstract:
Analysis of anxiety and depression among cardio-cerebrovascular disease patients in comprehensive hospitals
ZENG Gang, FU Chaowei, LUAN Rongsheng,
2006, 22(9): 1141-1142. doi: 10.11847/zgggws2006-22-09-69
Abstract(1293) HTML (247) PDF 367KB(39)
Abstract:
  Objective   To investigate the prevalence and its determinants of anxiety and depression among cardio-cerebrovascular disease patients of comprehensive hospitals in Chengdu city.   Methods   3 comprehensive hospitals were selected by convenience sampling method, among which 152 patients with coronary heart disease and 100 patients with stroke were collected continuously.All the subjects were assessed by Hospital Anxiety and Depression Scale(HADS), Hamilton anxiety scale (HAMA)and Hamilton depression scale(HAMD)by psychiatrist.   Results   5.6% paients were identified as depression, 6.7% as anxiety, 92.9% patients with depression as co-morbid of anxiety, 76.5% patients with anxiety as co-morbid of depression.No depression patient was diagnosed as depression, and received anti-depression treatment in the past, only 11.8% anxiety patients were diagnosed as anxiety and received anti-anxiety treatment in the past.Unemployment, current poor health condition and heavy work fatigue were risk factors for depression and anxiety.   Conclusion   The rate of the co-morbidity of anxiety and depression is quite high, and also the rate of diagnosis and treatment is pretty low.
2006, 22(9): 1143-1144. doi: 10.11847/zgggws2006-22-09-70
Abstract(822) HTML (178) PDF 265KB(37)
Abstract:
2006, 22(9): 1144-1144. doi: 10.11847/zgggws2006-22-09-71
Abstract(802) HTML (197) PDF 126KB(32)
Abstract:
2006, 22(9): 1145-1146. doi: 10.11847/zgggws2006-22-09-72
Abstract(1017) HTML (309) PDF 134KB(27)
Abstract:
Application of local indicators of spatial autocorrelation to analysis of disease cluster
CHEN Bingwei, XU Biyun, LI Deyun,
2006, 22(9): 1146-1147. doi: 10.11847/zgggws2006-22-09-73
Abstract(1039) HTML (322) PDF 410KB(44)
Abstract:
  Objective   To explore application of spatial autocorrelation and local indicators of spatial autocorrelation in disease.   Methods   Taking the evaluating data of iodine deficiency disorders(IDD)of Sichuan province in 2000, Moran's I statistic and local Moran's I coefficient of level of median of salt iodine were calculated, and the map of local Moran's I was made.   Results   The Moran's I statistic of salt iodine was 0.2560(P < 0.05), local Moran's I were bigger than 0 in most region. Conclusions On the whole, there was spatial cluster about salt iodine in Sichuan province and from local, and there still were some region whose salt iodine were different from neighboring region.
2006, 22(9): 1148-1150.
Abstract(568) HTML (142) PDF 467KB(41)
Abstract:
2006, 22(9): 1150-1150. doi: 10.11847/zgggws2006-22-09-75
Abstract(830) HTML (235) PDF 131KB(24)
Abstract:
2006, 22(9): 1151-1152. doi: 10.11847/zgggws2006-22-09-76
Abstract(859) HTML (204) PDF 200KB(25)
Abstract:
2006, 22(9): 1152-1152. doi: 10.11847/zgggws2006-22-09-77
Abstract(825) HTML (175) PDF 213KB(36)
Abstract: