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2009 Vol. 25, No. 4

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Assessment for immune effectiveness of hepatitis B vaccination among infant population in China
DANG Ru-bo, ZHANG Shun-xiang, ZHANG Wei-dong
2009, 25(4): 385-387. doi: 10.11847/zgggws2009-25-04-01
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ObjectiveTo analyze the effectiveness and benefits of strategy of hepatitis B vaccination implemented among infants for 14 years in China.MethodsOn the basis of available data,the health economic evaluation and decision tree model were determined to analyze system a tically the strategy of infant hepatitis B vaccination for 14 years in China.ResultsA total of 65 229 476 cases(24 423 516 cases in urban area,408 05 960 cases in rural area)hepatitis B virus infection,includding 13 045 894 acute patients,652 294 chronic patients,60 076 cases cirrhos is and 6 007 hepatoma were prevented because of the hepatitis B vaccination among the infants during 1992 through 2005 in China.The BCR was 51.01:1(49.59:1 in urban area,51.91:1 in rural area).ConclusionThe strategy of hepatitis B vaccination performed among the infants in China for 14 years obtained significant results.Decision tree is one of the most useful models to analyze the effectiveness of hepatitis B vaccination.
2009, 25(4): 387-388. doi: 10.11847/zgggws2009-25-04-02
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Epidemiological trend of hepatitis B, liver cirrhosis and liver cancer during 1990-2007 in Shandong province, China
QI Wen-ting, SUN Jian-dong, XU Ai-qiang
2009, 25(4): 389-391. doi: 10.11847/zgggws2009-25-04-03
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ObjectiveTo analyze the epidemio logical trend of hepatitis B,liver cirrhosis and liver cancer during 1990 to 2007 in Shandong province,and to evaluate the effectiveness of hepatitis B prevention and control measures,so as to provide the evidence for policy-making.MethodsBased on the routine incidence data of hepatitis B,mortality data of hepatitis B,liver cirrhosis,liver cancer and demographic data,the incidence rate,mortality rate and age-specific mortality rate were calculated and analyzed with simple linear regression model.ResultsA total of 437094 cases of hepatitis B were reported during 1990-2007 with an average yearly morbidity of 27.32 per 100 000 population and a decreased trend for the 0-9 year-old children.At the same time,the adjusted mortality rate for hepatitis Band liver cirrhosis showed a decreased tendency signifcantly and the combined mortality rate decreased from 17.55/100 000 in 1990 to 4.01/100 000 in 2007.While there was a steady tendency for liver cancer(P=0.999 8).ConclusionThe good immunization work of hepatitis B vaccine can lower the incidence of hepatitis B for the target population and the mortality rates of related liver cirrhosis and liver cancer.
Cytotoxic effect on HL-7702 cells induced by mercuric chloride
ZHANG Jie, JIN Ming-hua, WANG Hua
2009, 25(4): 392-394. doi: 10.11847/zgggws2009-25-04-04
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ObjectiveTo study the effects of mercuric chloride on cell proliferation,DNA damage,cell cycle and expression of MT in HL-7702 cells.MethodsThe MTT assay was used to detect cell proliferation.The DNA damage in cells was determined by SCGE assay.The changes in cell cycle was measured by FCM assay,and the expression of MT in cells was detected by mimunocy to chemistry assay.ResultsThe inhibition of cell proliferation and DNA damage induced by mercuric chloride were time-and dose-dependent(P<0.05).Significant decrease of cells in Sphase and increase in G0/G1 demonstrated that G0/G1 phase blocking and inhibition of DNA synthesis could be induced by mercuric chloride(P<0.05).In addition,the expression of MT increased significantly along with the increasing of doses(P<0.05).ConclusionMercuric chloride had toxic effect on HL-7702 cells.It could decrease activity of enzymes which conta in hydro sulfuryl, induce DNA damage and changes in cell cycles.
2009, 25(4): 394-395. doi: 10.11847/zgggws2009-25-04-05
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Positive selection of ORF1 and ORF2 in hepatitis E virus genome
SHAO Zhong-jun, LI Jing-hua, FAN Jun
2009, 25(4): 396-397. doi: 10.11847/zgggws2009-25-04-06
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ObjectiveTo explore positive selection sites in ORF1 and ORF2 of hepatitis Evirus(HEV)genome.MethodsThirty-one complete genomes of types of HEV were retrieved from GenBank,and were aligned,corrected by codon with MEGA 3.1 software package.Phylogenetic tress were constructed with PHYLIP3.6 package.Adaptive evolution was analyzed with PAML 4.0 package.ResultsIt was revealed that purified evolution prevailed the ORF1 and ORF2 with ω of 0.029 and 0.024 respectively.Ninety-three percent of ORF1 sites and 98 percent of ORF1 sites were conservative with ω of 0.018 by neutral evolution model.No positive selection site was found in ORF1 or ORF2.ConclusionNo positive selection site was found in ORF1 or ORF2,which indicated that positive selection was not prevailing during HEV evolution.
Cloning, expression and sequence analysis of cysteine-rich secretion protein 2 gene from Taenia saginata asiatica
DAI Peng, DAI Jia-lin, Huang Jiang
2009, 25(4): 398-400. doi: 10.11847/zgggws2009-25-04-07
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ObjectiveTo search and identify novel gene by screen ing the cDNA library of adult Taenia saginata asiatica, and to clone and express the gene so as to lay a foundation for the further study of Ta CRISP gene's function.MethodsBy the bioinformatics analyzing tools in bioinformatics webs site,a Ta CRISP 2 full-length gene from the Ta eniasa ginata asiatica full-length cDNA plasmid libratory was identified and the coding region sequence and the characteristics of the deduced prote in were analyzed.The coding region of Ta CRISP was amplified with PCR,endonuc lease digestion and cloned in to the prokaryotic expression vector pET-30a(+)and then expressed in E.coli BL 21 with IPTG induction.The recombinant protein was detected by SDS-PAGE.ResultsA novel cDNA sequence encoding Ta CRISP with a molecular mass of 269738,pI 5.96,which was 1090 bp and encoding 239 aa,and with 1aa to 16aa signal peptide,was found from the cDNA library of Taenia saginata asiatica.The recom binant plasmid pET-30a(+)-TaCRISP was constructed and expressed in fusion prote in.ConclusionA novel gene encoding TaCRISP of Taenia saginata asiatica was identified and its prokaryotic expression vectors was expressed in fusion protein successfully.
Effect evaluation of new rural cooprative medical system (NCMS) insurance fund for serious disease
HAN Ying, ZHENG Jian-zhong, LIANG Wei-ping
2009, 25(4): 400-401. doi: 10.11847/zgggws2009-25-04-08
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ObjectiveTo evaluate the efficiency and equity of NCMSs' fund in Jiangxi province.MethodsStatistic description,comparion analysis and Gini coefficient were involved in the study.ResultsThe compensate propotion of NCMS was 23.57% and 31.33% in Yushe and Loufan county,respectively,and it reached to 41.04% by recompensation.Revenus and expenditure of the fund were basically balanced.The participants suffering the heaviest disease got the most compensation(48.7%)from NCMS,but they were still with the heaviest economic burden.The rate of hospitalization for country man improved from 3.9% to 4.3%,the income Gini coffecient of the patients'families fell from 0.454 to 0.372.ConclusionThe efficency of NCMS fund is high,and vertical equity of the compensation could be observed.But the proect should be improved so as to reduce the disease burden of the disadvantage participants.
Effect of cooking on pesticide residues and application of cooking factors in dietary exposure assessment
LAI Jue-qiong, TIAN Zi-hua, PAN Kang-biao
2009, 25(4): 402-404. doi: 10.11847/zgggws2009-25-04-09
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ObjectiveTo study the removal effects of cooking on organophosporus pesticides in vegetables and the influece of cooking factors in dietary exposure assessment.MethodsThe concentrations of the pesticides were analyzed before and after cooking then cooking factors were calculated.Dietary exposure levels of the pesticides were calculated and compared with or without the consideration of cooking factors.ResultsThe residue levels of the organophosphorus pestitides were different for different kinds of pesticide (F=18.758, P<0.01),and the cooking factors were within the range of 0.06-0.88.According to the percentage of the pesticides removed, the sequence of the pesticides was acephate>chloipyri-fos>fenitrothion>dinethoate>triazophos.The longer the vegetables were cooked, the less residual levels of the organophosphorus pesticides left.The intake levels of organophosphosphorus pesticides were reduced with the consideration of cooking factors.ConclusionWith the consideration of the influence of cooking, more precise assessment for the dietary exposure can be achieved dietary exposure assessment.
Glycometabolism and function of pancreatic cell in type Ⅱ diabetes patients
YANG Guang, WANG Li-bo, WANG De-cai
2009, 25(4): 404-405. doi: 10.11847/zgggws2009-25-04-10
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ObjectiveTo explore the relationship between glycometabolism andpancreatic B cell in type Ⅱ diabetes.MethodsFifty-one patients with newly diagnosed type Ⅱ diabetes and forty-eight noun alpersons were selected.The fasting blood glucose, insulin, C-peptide and HbA1c were deteunfined with radioinm unoassay, enzyme-linked inmunosorbent assay and inmune nephelometry.ResultsFor newly diagnosed type Ⅱ diabetes patients, the values of fasting C-peptide, insulin, HbA1c and fasting blood glucose were higher than that of the nounal(P<0.01),and HbA1c and fasting blood glucose were positively correlated C lucose content changed wih diversification of C-peptide, insulin and HbA1c.Conclusion The value of fasting C-peptide, blood glucose, insulin and HbA1c of type Ⅱ diabetes patients are higher than that of the nounal D fiver sification of C-peptide, insulin, HbA1c can cause blood glucose elevation.
Cloning and prokaryotic expression of actin related protein 2/3 complex subunit 4 of Taenia saginata asiatica
WANG Jie, DAI Jia-lin, HUANG Jiang
2009, 25(4): 406-408. doi: 10.11847/zgggws2009-25-04-11
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ObjectiveTo clone,express and conduct aprelimina ry mimuno reacticity study on genes of Taenia saginata asiaticas' act in related prote in 2/3 complex subunit 4(Arp2/3).MethodsBased on the Arp2/3 sequence template in the library of cDNA of Taenia asiatica,a pair of special primer was synthesized to amplify the gene through PCR technology.The genes were cloned in to prokaryotic expression vector Pet-28α(+)inducing the target genes to conduct expression through IPTG in competent Escherichia coli BL21/DE3 processed by CaCl2.The products of the expression was identified with SDS-PAGE.As the expression took place in an inclusion body,SKL was used to achieve purified recombinant prote in and the immuno reacticity study was conducted with We stern blotting.ResultsPCR,double enzyme digestion and DNA sequencing indicated that pET 28α(+)and Arp2/3 recombinant plasmid was succe ssfully constructed.SDS-PAGE results showed that the gene expression took place in Escherichia coli BL21/DE3,and highly pure prote in was achieved after the dissolving,refolding and particle exchange chroma tography of inclusion body deposits.The recombinant prote inreacted with Taenia asiatica and taeniarhynchus saginatus infected patients' serum,which indicated the mimuno reacticity of the prote in.ConclusionThe Arp2/3 subun it 4 gene is successfully cloned.The recombinant prote in is obtained through expression and purification,and the genes' mimuno reacticity is confirmed,which provides the foundation for further studies of the gene.
2009, 25(4): 408-409. doi: 10.11847/zgggws2009-25-04-12
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Estimated burden of cardiovascular and cerebrovascular diseases in Zhejiang province
YANG Jing, SHEN Qing, TANG Xin-hua
2009, 25(4): 410-412. doi: 10.11847/zgggws2009-25-04-13
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ObjectiveTo estinate the burden of cardiovascular and cerebrovascular diseases in Zhejiang province.MethodsAccording to data ofmorbidity surveillance and epidemiological investigation in Zhejiang province in 2006, the disability-adjusted life expectancy (DALE) and disabled life expectancy (DLE) attributed to cardiovascular and cerebrovascular diseases were calculated following the procedures developed by Sullivan to estinate the burden of the diseases.Results27 391 participants were selected, including 13 965 male and 13 426 female.Life expectancy of residents in Zhejiang was 75.2 years DLE attributed to cardiovascular and cerebrovascular diseases in 0-age group was 2.05 years with the most contribution of hypertension.Adjusted for cardiovascular and cerebrovascular diseases,DALE in 0-age group was 73.15 years The burden of cardiovascular and cerebrovascular diseases in female residents was higher than that of in male residents, and the burden of cardiovascular and cerebrovascular diseases in urban residents was higher than that of in rural residents.ConclusionThe DLE of female and urban residents due to cardiovascular and cerebrovascular diseases is higher than that of male and rural residents.
2009, 25(4): 412-412. doi: 10.11847/zgggws2009-25-04-14
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Cloning, expression and purification of pan-allergen tropomyosin from Squilla oratoria and identification of its allergic activity
ZHAN Zheng-ke, LIU Zhi-gang, JI Kun-mei
2009, 25(4): 413-415. doi: 10.11847/zgggws2009-25-04-15
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ObjectiveTo clone the tropomyosin gene from squilla oratories and produce its recombinant protein and investigate its allergenicity.MethodsThe cDNA of tropom yosin was cloned using specific priners from the total RNA of squilla oratories The cloned gene was inserted into pMD 18-T vector and digested by Nde I and Hind Ⅲ The cDNA was sequenced and then subcloned into pET-28a expression vector The cloned tropom yosin cDNA gene was expressed in Escherichia coliBL21 (DE3) by IPTG induction.The recombinant tropomyosin was purified by metal (Ni2+)chelating affinity chromatography.The allergenicity of the recombinant tropomyosin was examfined by western blotting method.ResultsThe cloned cDNA ORF sequence contained 855 by and encoded 284 aminoacids (GenBank database entry Na EF584510).The recombinant allergen tropomyosinwas highly expressed in E.coli BL21 (DE3) with the molecular weight of about 36 kDa under induction of IPTG and purified by 6-His-tag purification system.And the recombinant allergen was identified as its affinny to IgE antibodies from the shrinp-allergic patient sera.ConclusionRecombinant squilla oratories tropomyos in with good allergenicity was obtained in this study.
Relationship between pre-pregnancy body mass index, gestational weight gain and health of mother and infant
JIN Zi-huan, MA Shu-xiang, DONG Li-hong
2009, 25(4): 415-416. doi: 10.11847/zgggws2009-25-04-16
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ObjectiveTo investigate the influence of the nounal pregnant woman's pre-pregnancy body mass index (BMI) and gestational weight gain on the health of mother and infant.MethodsThree hundreds and sixty cases of monomoiphic priniparas were selected and divided according to the pre-pregnancy BMI and the gestational weight gain into groups of no more than 10, 10-15, 15.5-20,20.5-25kg, and more than 25.5kg A 11 their pregnancy outcomes were followed up.ResultsThe cesarean section rate and neonatal weight of the pre-pregnancy obesity group had no difference compared with the pre-pregnancy noun alweight group(P>0.05).The occurrence rate of pregnancy induced hypertension and gestational diabetes mellitus of the pre-pregnancy obesity group and the group with pregnancy weight gain was more than 15.5kg showed significant increase(P<0.O5).The cesarean section rate of pregnant women with gestational weight gain more than 10.Skg showed an evident increase among the obesity group (P<0.O5).The cesarean sectionmte of those with pregnancy weight gain more than 20.Skg had an evident increase in the nounal group(P<0.05).The gestational weight gain was not related to the neonatal weight(P>0.05).ConclusionThe pre-pregnancy body mass index is a risk factor for the occurrence of pregnancy complications.Excess gestational weight gain is related to pregnancy outcoma and reasonable diet control during pregnancy can not influence the neonatal weight.
Reverse effects of α-NF pre-treatment on protein levels of P53, P21 in HELF cells induced by B(a)P
XU Yong-jun, BAI Xue-tao, ZHANG Hong-wei
2009, 25(4): 417-419. doi: 10.11847/zgggws2009-25-04-17
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ObjectiveTo investigate the effects and mechanisms of B(a)Pon P53,P21 and AhR pathway of HELF cells in vitro.MethodsDifferent doses of α-NF,AhR an tagonist were used to pre-treat human diploid lung fibroblast (HELF)cells for 1h,followed by B(a)Ptreatment for additional 2h with or without S9,or followed by B(a)P treatment for additional 24h without S9.The prote in levels of P53,P21 were detected by western blot to investiga te AhR pathway's role involving in the effects of B(a)P on P53,P21.ResultsPre-treatment with α-NF at doses of 2.5 mol/L or 5.0 mol/L could reverse the increase of P53 levels with B(a)P treatment for 2h without or with S9,respectively.Pre-treatment with α-NF at doses of 2.5 mol/L and 5.0 mol/L could reverse the increase of P53 levels with B(a)Ptreatment for 24h without S9.Pre-treatment with α-NF at doses of 2.5 mol/L and 5.0 mol/L could reverse the increase of P21 levels with B (a)Ptreatment for 24h without S9.ConclusionP53,P21 play important roles in the response of HELF to DNA damage in duced by B(a)P,in which AhR pathway takes part in there sponse.
Effects of Yiqijiedu formula on cell proliferation and genes expressive levels of epithelia in mice
HE Ying-chun, TIAN Dao-fa, LU Fang-guo
2009, 25(4): 419-421. doi: 10.11847/zgggws2009-25-04-18
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ObjectiveTo investiga te the effects of Yiqijiedu formula on cell proliferation of nasal and nasopharyngeal epithelia and the expressivelevels of TRAF2 and p16 in TgN(p53mt-LMP1)/HT mice.MethodsThe cell proliferative characteristics of nasalm uco sa and na sopharyngeal epithelia was evaluated by H-E staining procedures and the expressive levels of TRAF2 and p16 genes were assayed by immunohistochemical methods.At the same time,the genes expressive levels were also identified by western blot. ResultsCompared with tho se of TgN(p53mt-LMP1)/HT mice of normal saline (NS)group(TIC group),hyperp lasia of nasal and/or nasopharyngeal epithelia was not obvious in TgN(p53mt-LMP1)/HT cancerous lesion inducing mice with Yiqijiedu fromula(TMI group)and the occurringrate of precancerous lesions in nasal and/or nasopharyngeal epithelia was reduced significantly(P<0.01).The TRAF 2 expressivelevels(129.45±9.53, 160.96±11.04 of nasal cavity epithelia of TIC group,and TIM group;126.36±12.69,160.88±6.84 in naso phary geal epithelia)we re decrea sed(P<0.01)and p16 expressivelevels(157.21±10.44,131.72±10.06 in nasal epithelia of TIC and TIM group;155.41±11.77,132.63±10.22 in na sopharyngeal epithelia of TIC and TIM group)were increased markedly(P<0.01).TRAF2 and p16 expressive levels were similar to those of C57 BL/6J wild type mice(TRAF2 gene: 165.07±10.58 in nasal epithelia,164.39±7.88 in naso phary geal epithelia;p16 gene:129.62±12.16 in nasal epithelia and 129.12±11.67 in nasopharygeal epithelia)with no significant differece.ConclusionThe effects of N,N'-dinitro sopipera zine(DNP)on nasal/naso phary ngeal epithelial abnormal proliferation of TgN(p53mt-LM P)/HT mice can be inh ibited by Yiqijiedu formula herbal medicine.The effects of Yiqijiedu fromula is closely related with TRAF2 gene down regulation expression and p16 gene expression upregulation in nasal/naso pharyngeal epithelia of TgN(p53mt-LMP1)/Ht mice.
Protective effect of curcumin on damage of HaCaT cells irradiated with UVB
KANG Shun-ai, GUO Cai-xia, WANG Zhi-cheng
2009, 25(4): 422-423. doi: 10.11847/zgggws2009-25-04-19
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ObjectiveTo study the effect of curcum in(Cur)on the imto chondria and some correlation facto rs in HaCaT cells irradiated with UVB and its protection effect on the cells damaged by UVB.MethodsAfter different doses of Cur were added into HaCaT cells irradiated with UVB,the cells were continually cultured.The mitochondria membrane potential(Δψ)and the free intracellular calcium([Ca2+]i)concentration were measured with Rhodamine 123 and Fluo-3/AM fluo rescence,respectively.The prote in expressions of caspase-3 and cytochromc(Cytc)were examined by flowcy tometry.ResultsAs compared with tho sein the normal control group after UVB irradiation,all indices increased significantly(P<0.01).The cell population of olig-Δψ potential was 80.8±2.23%;the[Ca2+]i concentra tion was 71.9±1.61% and the prote in expressions of caspase-3 and Cytc were 29.00±1.60% and 27.5±1.60%,respectively.As compared with tho sein the UVB group,a down-regulation dose-effects of Cur in the Δψ potential and[Ca2+]i concentration and the prote in expressions of caspase-3 and Cytc were shown.ConclusionThe results suggest that Cur could inhibit HaCaT cell apoptosis caused by UVB irradiation through the increase of Δψ potential and the decreases of the[Ca2+]i concentration and the cascade reaction of Cytc and caspase-3,and have protective effect on HaCaT cells damaged by UVB.
Cross-sectional study on house decoration and its health effects in four cities of China
ZHANG Wen-li, FANG Xin, CAO Zhao-jin
2009, 25(4): 424-426. doi: 10.11847/zgggws2009-25-04-20
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ObjectiveTo explore the association between house decoration and residents' health.MethodsUsing simple cluster sampling method,subjects living in Beijing,Tianjin,Shanghai and Nanjing cities in China were selected and detail information on house decoration and family member's health status was collected.ResultsAmong 6 681 residents interviewed,4 008(60.0%)reported different severe discom for table symptoms after living in the new decoration house.The interval from living in the house to the date decoration finished and the time stayed in the decorated house were risk factors of residents' ill symptoms.Decoration materials including hydrophilic dope,wall paper,wooden floor,tile and marble were protective factors,but compound floor,chemical fiber carpet and leather floor were risk factors.Opening kitchen and gas using were risk factors.However smoke evacuation system and ventilation by opening windows were protective factors.ConclusionDifferent severe discom for table symptoms appear after living in new decoration house among the residents.The discom for table symptoms are the result of many decoration factors combined together.
Effects of curcumin on proliferation of hepatic satellite cell in vitro
JI Hui, REN Li-qun, ZHANG Yi-ning
2009, 25(4): 426-427. doi: 10.11847/zgggws2009-25-04-21
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Objective To investigate the effects of curcumin on proliferation and collagen(col)secretion of hepatic satellite cells in vitro.Methods CSFSC-2G were incubated with different concentration of curcumin(0,5,10,15,20,40 μmol/L)for different times(6h,12h,24h,48h and 72h).MTT was used for the detecting of proliferation of HSC.Col Ⅰ and Col Ⅲ were determined by ELISA and flowcytometry was applied to evaluate apoptosis of HSC.Results Curcumin inhibited the proliferation of HSC with dose-dependent manner,especially in 20 and 40 μmol/L curcumin groups at 24h.When HSC was cultured after 24h,the absorbency of Col Ⅰ and Col Ⅲ in 20 μmol/L curcumin group was 0.418±0.019 and 0.554±0.043,respectirely,both lower than those of 0 μmol/L curcumin group significantly(P<0.01).When HSC was cultured after 48h,the ratio of apoptosis in 20 μmol/L curcumin group was 20.31%±2.55% and higer than that of 0 μmol/L curcumin group significantly(P<0.01).Conclusion Curcumin can delay liver fibrosis not only by inhibiting the proliferation and stimulating apoptosis of HSC,but also by decreasing Col Ⅰ and Col Ⅲ secretion.
Study on SOCS-3 and FAS expression of adipose tissues in rats fed with high-fat diet
LIU Li, MA Shuang, LI Yan-xi
2009, 25(4): 428-430. doi: 10.11847/zgggws2009-25-04-22
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ObjectiveTo study the expression of suppress or of cytokine signaling 3(SOCS-3)and fatty acid synthase(FAS)mRNA of adipose tissue in obesity and obesity resistant rats induced by high-fat diet.MethodsSeven male Wistar rats were fed with standard diet as control group and 24 rats fed with high-fat diet.After 8 weeks,5 rats as obesity group and 5 rats as obesity resistant group were selected by body weight increment from high-fat diet group.Serum triglyceride,total cholesterol,SOCS-3 and FAS mRNA of epididymal adipose tissues were detected.ResultsSerum triglyce ride (0.982±0.228 mmol/L)and total cholesterol level(2.213±0.364 mmol/L)of obesity group were significantly higher than that of control(0.717±0.153,1.784±0.175 mmol/L,P<0.05),and total cholesterol level was higher than that of obesity resistant group(1.711±0.190 mmol/L,P<0.05).SOCS 23 and FAS mRNA of obesity group was also higher than that of control and obesity resistant group(P<0.05).ConclusionObesity rats and obesity resistant rats induced by highfat diet might be different in gene regulation,and obesity rats show both increased fat synthes is and possible suppression of leptin signaling transduction.
2009, 25(4): 430-430. doi: 10.11847/zgggws2009-25-04-23
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Analysis of polycyclic aromatic hydrocarbons, organic carbon and element carbon in air particulate matter in Beijing
CUI Rong, GUO Xin-biao, DENG Fu-rong
2009, 25(4): 431-432. doi: 10.11847/zgggws2009-25-04-24
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ObjectiveTo investigate the pollution characteristics of polycyclica romatic hydrocarbons(PAHs),organic carbon(OC)and element carbon(EC)in PM2.5 and PM10.MethodsThe PM2.5 and PM10 samples collected from June 16 to 18 and from June 20 to 22 of 2006 in Beijing were measured and 17 kinds of polycyclica romatic hydrocarbons,organic carbon and element carbon were analyzed.ResultsThe average mass concentrations of PAHs in PM2.5 and PM10 we re 0.011-2.846 ng/m3 and 0.013-4.415 ng/m3,respectively.The average mass concentrations of OC and EC were 28.56 μg/m3,8.75μg/m3 and 41.14μg/m3,15.43μg/m3 in PM2.5 and PM10,respectively.ConclusionIn PM2.5 and PM10,4 to 5 rings PAHs we re the main components of 17 kinds of PAHs,and the total concentration of OC and EC accounted for the similar percentage.Carbon was one of the main components of PM2.5 and PM10 in the air sample in Beijing.
Analysis of psychological health status of university postgraduate students and its influencing factors
FANG Wei, YANG Xing-fen, WANG Sheng-yong
2009, 25(4): 433-435. doi: 10.11847/zgggws2009-25-04-25
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ObjectiveTo analyze the psychological health status of postgraduate students and to find out the factors associated with psychological health.MethodsA purposive samele of postgraduate students from a university of Guangdong province was surveyed by measuring the scale of their psychological stress with SCL-90.ResultsThe scores of SCL-90 factors were lower than that of the noon of college students while some factor scores were higher than the national noon of youth.The top three positive factors were obsessive-compulsive(25.2%),depression(18.9%),interpersonal sensitivity (18.4%).The results of Logistic regression analysis showed that bad relations with roommates, bad relation with spouse or lover, failure in completing the study plans, emotional volatility, suffering from disesteem or discrinination and lack of career planning were risk factors.ConclusionThere exist varying degrees of psychosomatic problems in the special community of students.Mental health education should be enhanced among them.
2009, 25(4): 435-435. doi: 10.11847/zgggws2009-25-04-26
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Quality of life evaluation with SF-36 Chinese version in patients with different cardiac pacemaker implantation
ZU De-ling, ZHUGE Yi, TU Xiao-ming
2009, 25(4): 436-438. doi: 10.11847/zgggws2009-25-04-27
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ObjectiveTo evaluate the quality of life of the patients with different mode of cardiac pacemaker implantation with the SF-36 Chinese version.MethodsOne hundred and twenty-two cases of patients with different mode of permanent pacing were investigated before and after the operation with the SF-36 Chinese version.ResultsSuccessful surgeries on 121 cases were carried out and surgery on one case was unsuccessful.The original cardiac symptoms of 121 patients were improved in different degress after the operation.The scores of SF-36 in 8 aspects of the patients after the operation was higher than that of before the operation(P<0.01 or P<0.05)except for body pain dimension.The preoperative and postoperative scores of the patients with single lumen pacemaker were as following:physical functioning,54.1±20.5 vs 66.8±19.4(P<0.01);role-physical,26.4±11.6 vs68.8±31.6(P<0.01);bodily pain,94.3±17.7 vs 96.8±19.7(P>0.05);general health,26.5±15.4 vs 50.3±23.5(P<0.01);vitality,46.2±10.9 vs 56.7±15.5(P<0.01);social functioning,35.4±11.8 vs 52.9±17.6(P<0.01);role emotional,22.5±14.4 vs 66.1±19.8(P<0.01);mental health,46.3±9.6 vs 58.3±13.8(P<0.01);reported health transition,40.1±12.5 vs 56.7±19.2(P<0.01).The preoperative and postoperative scores of the patients with dual chamber pacemaker were:physical functioning,60.3±14.2 vs 75.4±13.1(P<0.01);role-physical,25.0±11.3 vs 71.9±27.2(P<0.01);bodily pain,95.3±17.7 vs 97.8±19.7(P>0.05);general health,97.8±19.7 vs 58.1±15.9(P<0.01);vitality,48.2±15.7 vs 63.8±13.4(P<0.01);social functioning,38.3±12.7 vs 60.6±15.4(P<0.01);role emotional,17.1±8.3 vs 61.9±30.1(P<0.01);mental health,48.6±8.3 vs 64.5±11.5(P<0.01);reported health transition,42.7±9.1 vs 64.4±16.9(P<0.05),respectively.There were better therapeutic efficacies in patients with dual chamber pacemaker than those with single lumen pacemaker.ConclusionThe quality of life of patients with permanent pacemaker is improved in physiologic and psychosocial function after the operation.It is important to select physiological pacing mode according to patient's condition to enhance the quality of life of the patients after the operation.
Relationship between mental health status of pregnant women and low birth weight neonates
JIANG Xiao-min, TAO Fang-biao, DONG Juan
2009, 25(4): 438-440. doi: 10.11847/zgggws2009-25-04-28
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ObjectiveTo describe mental health status in pregnant women and to estinate whether there is an association between psychosocial stress and low birthweight.MethodsA voluntary sample of 1 519 pregnant women who had the forcoming birth in a hospital in Hefei was selected.Their social demography and mental health infounanon were assessed by a questionnaire survey and were compared between women who delivered low birth weight neonates and women who deliverect nounal birth weight neonates.Chi square test relative risks and 95% confidence intervals were calculated by means of non-conditional Logistic regression.ResultsLow birth weight rates were different in different maternal registered peonanent residence, age and educational degree, and the differences were of significance(P<0.05 for all comparisons).There was no significant association between maternal anxiety and a low birth weight delivery, and the analysis of maternal depression, trait coping style, social support yielded the same results(P>0.05 for all comparisons).After adjusting formaternal social demogmphy and complicated disease factors, modemte or severe life events(odds ratio=3.619, 95% confidence interval: 1.863-7.028) was significantly associated with low birth weight delivery(P=0.000).ConclusionThis study provides evidence thatmoderate or severe psychosocial stress in pregnantwomenmay have adverse effect on fetal growth.
Influences of gender and age on correlation of BMI and BF% in adult
HE Ying-jian, TAO Qiu-shan, LI Xiao-ting
2009, 25(4): 441-443. doi: 10.11847/zgggws2009-25-04-29
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ObjectiveTo explore the correlation between body mass index (BMI) and percentage of body fat(BF%) and the inpact of gender and age on the correlation. MetbodsData from 78, 257 participants in 2006 annual health screening were used for data mining, correlation analysis, linear regression and agreement analysis. Results There was a moderate correlation between BMI and BF% (r=0.641, P<0.001).However, close correlations in bothmale and female(r=0.808, r=0.925, P<0.001, respectively) were found.It suggested based on partial correlation coefficient that gender had much more inportant inpact on the correlation coefficient between BMI and BF% than age. The linear regression analysis suggested that the cut-off points of BMI for ovenv eight and obesity were 22 and 24.5 in male, 19.5 and 22 in female aged less than 30; 24 and 27 in male, 21 and 25 in female aged more than 30, respectively. The agreement rate was 59.2% when original diagnostic standard of BMI was used, with a Kappa coefficient of 0.379±0.003(P<0.001).The agreement rate was 73.6% when new diagnostic standard of BMI was used, with a Kappa coefficient of 0.588±0.002(P<0.001).Twice more ovenveightpopulation could be screened out by the new standard than the original one. ConclusionBMI would be a betterm easurement of body fatty ith new cut-off points established for different gender and age.
2009, 25(4): 443-444. doi: 10.11847/zgggws2009-25-04-30
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Study on genetic association between the polymorphism of KPNB1, KPNB2 and schizophrenia
LIU Li-bo, JU Gui-zhi, SHI Jie-ping
2009, 25(4): 445-447. doi: 10.11847/zgggws2009-25-04-31
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ObjectiveTo investigate the association between schizophrenia and thepolymorphism of karyopherin beta1 (KPNB 1) and karyopherin beta-2(KPNB 2).MethodsPCR-restriction fragment length polymorphism (RFLP) was used to examine two single nucleotide polymorphisms(SNPs) of rs11871606 at the KPNB1 locus and rs266443 at the KPNB 2 with 233 nuclear family trios of schizophrenia.The Hardy-Weinberg equilibrium for genotyp is distribution was estimated by the goodness-of-fit test.The UNPHASED program was applied to perfoun the transmission disequilibrium test (TDT) and the combined effect analysis of the paired SNPs.ResultsThe genotype frequency distributions of hvo SNPs were in accordance with the Hardy-Weinberg equilibrium.The results of TDT indicated that the allelic frequency tmnsm fitted from the heterozygote parents did not deviate from 50%.The results of the combined effect analysis showed that rs11871606-rs266443 was not associated with schizophrenia.ConclusionAs far as Chinese Han schizophrenia concerned in this paper, we may not be assured the association between the polymorphism of KPNB 1 or KPNB 2 and schizophrenia.
Effects of hypoxia exposure on sperm quality and intervention of compound Codonopsis pilosula
CUI Jian-hua, WANG Yin-hu, GAO Liang
2009, 25(4): 447-448. doi: 10.11847/zgggws2009-25-04-32
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ObjectiveTo investigate the effects of hypoxia exposure on speun quality and intervention of compound Codonopsis pilosula.MethodsThirty-seven youth inm igrated to high altitude(5200m)for 1 year(HA group) were examfined for speun iation quantity, speun density, colliquation time, activity rate, motoricity, seminal plasm a fructose,α-glucosiBase, AC-PH, acrosomal enzyme, zinc and compared with the Champaign group (n=13).Subjects in HA group were divided into compound Codonopsis pilosula group (n=18) and placebo group(n=19) then taken compound Codonopsis pilosula or placebo twice per day for 3 months Speun samples were collected and detected after the drug with drawal.ResultsSpeun activity rate, density, sem final plasm a fructose,α-glucosidase, AC-PH, acrosomal enzyma and zinc in HA group were significandy lower than those of Champaign group (P<0.01 or P<0.05).Speun activity rate, plasm a fructose and acrosomal enzyme in placebo group were significantly lower than those of Champaign gomp(P<0.01).Acrosomal enzyme and zinc increased significantly in compound Codonopsis pilosula group compared with the placebo group(P<0.05).ConclusionSpeun quality can degrade after living at high altitude for 1 year and can't complete recovery after returning to plain for 3 months and compound Codonopsis pilosula has some effect on sperm quality recovery.
Atorvastatin inhibits inflammation in experimental diabetes mellitus rats
DU Jing, YANG Chao, LU Hong
2009, 25(4): 449-451. doi: 10.11847/zgggws2009-25-04-33
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ObjectiveTo investigate the effects of atorvastatin on the inflammation in streptozotocin(STZ)-induced type I diabetic rats.MethodsDiabetes mellitus was induced in 8 weeks old male Sprague Dawley(SD)rats by two times intraperitoneal injection of STZ.Low dose of atorvastatin,which had no effects on the serum lipid profile,were administered to diabetic rats.Fasting plasma glucose and serum concentrations of triglyceride,total cholesterol,low density and high density lipoprotein,tumour necrosis factor-α(TNF-α),C-reaction protein(CRP)and interleukin 1β(IL-1β)were measured.In aortic tissue,the expression of TNF-α,IL-1β,vascular cell adhesion molecule-1(VCAM-1)and intercellular adhesion molecule-1(ICAM-1)mRNA were analyzed respectively.ResultsThe levels of serum TNF-α,CRP and IL-1β in STZ-induced type I diabetic SD rats(SD-STZ)group were 2.118±0.153 μg/L,2.857±1.032 mg/L and 0.2347±0.1264 μg/L respectively,which were higher than those of SD control(SD-Co)group(P<0.05).In aortic tissue,the expression of TNF-α,IL-1β,VCAM-1 and ICAM-1 mRNA were significantly upregulated compared to SD-Co group(P<0.05).After atorvastatin administration,the levels of serum TNF-α,CRP and IL-1βwere greatly decreased which were 1.602±0.145 μg/L,2.263±0.691 mg/L and 0.156 5±0.037 μg/L,respectively.The expressions of TNF-α,IL-1β,VCAM-1 and ICAM-1 mRNA in aortic tissue were significantly downregulated compared to SD-STZ group(P<0.05).ConclusionLow dose of atorvastatin inhibits the inflammation in STZ-induced type I diabetic rats.Anti-inflammatory effects is not related to the cholesterol-lowering action of atorvastatin.
Association of leptin receptor gene mutation with BMI and serum lipid levels in hyperpietic population of China
LIU Hong, GAO Zhi-dong, CHEN Da-fang
2009, 25(4): 451-453. doi: 10.11847/zgggws2009-25-04-34
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ObjectiveTo investigate the association of leptin receptor gene(LEPR)A223G polymorphism with BMI and serum lipid levels in hyperpietic population of China.MethodsThe subjects were collected in Huangpu district in Shanghai,China.All subjects were subjected to a standard questionnaire and physical examination.PCR-RFLP method was used to detect the genotypes of A223G mutation.Obesity was classified as BMI≥24 kg/m2.ResultsNine hundred and two hypertension sufferers were eligible for the analysis.TG and TG/HDL levels were significantly higher and HDL level was significantly lower in obesity group than those in normal body weight group(P<0.05).The interaction of A223G mutation genotype and obesity were statistically significant in HDL(95% CI=0.0-0.1,P=0.042).ConclusionThe results indicate that LEPR A223G mutation is associated with serum lipid levels and it can modulate the relationship between obesity and serum HDL level.
Study on joint effect of welding fume and noise on hearing impairment
WU Lei, ZHU Chang-cai, YE Fang-li
2009, 25(4): 454-455. doi: 10.11847/zgggws2009-25-04-35
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ObjectiveTo evaluate the joint effect of welding fume and noise exposure on hearing.MethodsThe audition test was conducted among three groups of workers,including 65 workers exposed to both weding fume and noise environment,59 workers only exposed to noise and 55 unexposed workers.ResultsThe incidence of hearing impairment in joint group was 61.54%.The high-frequency threshold,speech hearing threshold,hearing impairment level Ⅰ,Ⅱ,Ⅲ and above hearing loss rates were 60.00%,35.38%,16.92%,23.08% and 20.00%,respectively.All differences between the joint group and the control group were statistically significant(P<0.05).Comparing the mean of hearing threshold in each group at different frequency band,the threshold of audibility on 0.5,1,2,3,4,6kHz was 22.98±6.28dB(A),21.38±7.88dB(A),22.04±10.19dB(A),26.55±16.69dB(A),31.52±18.86dB(A)and 33.75±19.71dB(A),respectively.The threshold of audibility on 0.5,1,2,3,4,6kHz was significantly increased in the joint group than those in noise group and control group(P<0.05).ConclusionWelding fume and noise exposure might increase hearing impairment in workers.
Influencing factors of IPSS and Qmax in middle aged and old male patients with essential hypertension
ZHANG Xiao, MAO Guang-yun, LIU Hai-peng
2009, 25(4): 456-458. doi: 10.11847/zgggws2009-25-04-36
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ObjectiveTo explore the influencing factors of international prostate syndrome score(IPSS)and maximum flow rate(Qmax)in middle aged and old male patients with essential hypertension in rural community.MethodsA population-based epidemiological investigation was conducted to collect 435 hypertensive male patients of 50 to 75 years old in Anqing,Anhui.Logistic regression was used for analysis of influencing factors.ResultsLogistic regression analysis showed that the main influencing factors of IPSS were age(OR 2.25,95% CI=1.45-3.48)and post void residual(OR 2.98,95% CI=1.92-4.63);the main influencing factors of Qmax were age(OR 2.22,95% CI=1.43-3.45),prostate volume(OR 2.39,95% CI=1.42-4.04),post void residual(ORR 2.09,95% CI=1.34-3.25)and smoking(OR 0.56,95% CI=0.31-0.99).ConclusionAge and post void residual are main influencing factors of IPSS.Main influencing factors of Qmax are age,prostate volume,post void residual.Cigarette using may reduce the risk for the decreased level of Qmax.
Association between polymorphisms of phase Ⅱ metabolic gene NQO1 and susceptibility to lung cancer
SU Jia, NIU Run-gui, HAN Xiao-you
2009, 25(4): 458-460. doi: 10.11847/zgggws2009-25-04-37
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ObjectiveTo explore the relationship between the polymorphisms of phase Ⅱ metabolic gene NQO1 codon 187 and the susceptibility to lung cancer and to evaluate possible interaction between NQO1 and sm oking with the risk of lung cance.MethodsA case-control study including 396 new diagnosed lung cancer cases and 465 healthy resident controls was conducted The PCR-based restriction fragment lengthpolymorphism (RLFP) techniquewas applied to analyze the NQO1 polym orphism at Pro187 Ser loci.The data were analyzed with logistic regression model.ResultsThe frequency of allele Pro and Ser in case group was 50.88% and 49.12%,respectively; while in the control group, it was 56.13% and 43.87%.A statistical significant difference was observed(P=0.0296).The carriers of Ser/Ser had higher risk of lung cancer than Pro/Pro carriers(adjusted odds ratio [AOR]=1.53, 95% confidence interval[95% CI]=1.O1-2.32).Moreover, the carriers of Ser/Ser had a much higher risk of adenocareinom a than Pro/Pro carriers (AOR=2.16, 95% CI=1.16-4.02).The smokers who carried mutative genotype of Ser/Ser possessed a 1.5 tines higher risk of lung cancer than the smokers who carried at least one wfill allele Pra.Furthermore, significant synergistic effect was seen between smoking and mutative genotype of NQO1 (Ser/Ser) in the occurrence of adenocarcinom a (AOR=2.74, 95% CI=1.00-7.49).Conclusion.The results suggested that genetic polym orphism of phase Ⅱ metabolic gene NQO1 colon 187 might play an inpor tant role in the development of lung cancer.Furthermore, smoking might hold synergistic effect with the polym orphisms of NOO1 colon 187 in the occurrence of lune cancer.
Study on protective effects of lutein on acute lung injury induced by lipopolysaccharide in mice
PEI Ling-peng
2009, 25(4): 461-463. doi: 10.11847/zgggws2009-25-04-38
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ObjectiveTo study the protective effects of lutein against acute lung injury induced by lipopolysaccharide and its possible mechanism in mice.MethodsRandomly distributed 60 male Kunming mices into control group,lipopolysaccharide-induced acute lung injury model group(ALI),dexamethasone(DXM)group(5 mg/kg)and low,middle,high dose groups of lutein(10,15,20 mg/kg).After 30d with different dosage of lutein,control group was given physiological saline,ALI model group,lutein administrated groups and DXM group were injected with lipopolysaccharide(LPS)(6.0 mg/kg)to induce ALI.Six hours after the injection,abdominal aorta blood was collected for measuring of lymphocyte subpopulations(CD3+,CD4+,CD8+),tumour factor-α(TNF-α),leckocyte interpose-8(IL-8),malondialdehyde(MDA)content,superoxide dismutase(SOD),glutathione perioxidase(GSH-Px)activities.And lung wet weight/dry weight ratio,neutrophil MPO activity of lung TNF-α,leckocyte interpose-10(IL-10)content of lung were measured.ResultsTreatment with different dosage of lutein could significantly decrease serum TNF-α(model group:475.29±93.12;lutein groups from low dose to high dose:390.10±81.50,374.20±80.09,340.18±84.39);IL-8(model group:124.56±20.21;lutein groups from low dose to high dose:111.13±16.30,107.33±15.39,103.39±14.36);MPO(model group:6.02±1.06;lutein groups from low dose to high dose:5.12±1.02,5.03±0.80,4.48±0.73);MDA content(model group:11.28±2.14;lutein groups from low dose to high dose:9.20±0.62,8.29±1.30,7.10±1.21).There was a reduction of IL-10 in lung(model group:60.13±20.28;lutein groups from low dose to high dose:71.23±22.39,78.28±21.27,85.18±28.15).The concentrations of SOD were(model group:74.52±24.40;lutein groups from low dose to high dose:114.30±41.50,130.53±40.23,149.19±41.77).GSH-Px activities were(model group:62.24±10.13;lutein groups from low dose to high dose:77.70±12.15,85.20±12.03,90.47±13.12).The lymphocyte subpopulations of the blood was improved.ConclusionLutein showed protective effects on the acute lung injury induce by LPS in mice.
Influence of UVB irradiation on oxidative damage of experimental silicosis rat
SONG Qi-ru, LI Hong-cheng, WANG Fa-xuan
2009, 25(4): 464-466. doi: 10.11847/zgggws2009-25-04-39
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ObjectiveTo explore the influence of UVB irradiation on oxidation damage of experimental silicosis rat. MethodsThirty two SD rats were randomly divided in to four groups.Rats in the experiment were injected with SiO2 and irradiated with UVB.The rats were killed and their blood and lung tissue were collected.The contents of SOD,MDA and HYP in 10% homogenate of lung tisue and the contents of CP in serum were detected by colourometry.Body weight gain and organ weights were determined.ResultsAfter in jecting SiO2 ,the activities of SOD in lung tissues homogenate were decreased(P<0.01)and the contents of HYP of lung were increased(P<0.01).After injecting SiO2,the serum CP activities and the contents of HYP were higher than non injected rats(P<0.05).After irradiated with UVB,the activities of SOD in lung tissues homogenate were decrea sed(P<0.01).The changes of MDA ,HYP and CP were not statistically significant(P>0.05).After the exposure of UVB and silica particles,SOD and HYP in lung tissue homogenate and CP in serum showed interaction with the exposure(P<0.01).MDA in lung tissues homogenate showed interactions with the treatment(P<0.05).ConclusionSiO2 has oxidation effect on pumlonary tissue of experimental silicosis rats and can cause pumlonary fibrosis.But UVB exposure could decrease the oxidation effect and mitigate pumlonary fibrosis.
Curative effect of EDTA on kidney injury caused by chronic lead poisoning
SU Su-hua, LIAO Rui-qing, NONG Kang
2009, 25(4): 466-467. doi: 10.11847/zgggws2009-25-04-40
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ObjectiveTo study the protective effect of sodium calcium edetate(EDTA)on kidney injury in chronic lead poisoning patients.MethodsThe 52 chronic lead poisoning workers were treated with EDTA for 3 years.The blood lead(PbB),erythrocyte zinc protoporphyrin(ZPP),urine β2-microglobulin(U-β2-MG),urine retinal conjugated protein(U-RBP),urine β-N-acetylyluco-samidase(U-NAG)of the workers were dynamically detected.ResultsAll of the indices of poisoning worker were higher than that of control group before EDTA treatment(P<0.01).After continuously treatment of EDTA,the values of the indices decreased year by year and the differences in the values between each year were significant(P<0.01).ConclusionThe EDTA treatment is protective to the kidney injury in chronic lead poisoning patients.
Study on germicidal and related proprties of ortho-phthalaldehyde disinfectant
HU Guo-qing, XU Yan, PAN Xie-shang
2009, 25(4): 468-469. doi: 10.11847/zgggws2009-25-04-41
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ObjectiveTo study the geunicidal efficacy of ortho-hthaladehyde disinfectant and its related properties.MethodsB actericidal quantitative suspension test, stinulating practical conditions and field test, stability test and metal corrosion testwere used.ResultsThe logarithm reduction of Escherichia coli and Staphylococcus aureus exposed to the disinfectant solution containing 0.53% ortho-phthalaldehyde fort.5 min were larger than 5.00, and that of Candida albicans and M.Chelonae subsp, abscessus were larger than 4.00.It could reach a high disinfection level in endoscope disinfection stinulating practical test and field testes ith 0.53% ortho-phthalaldehyde for 5m in.There was no corrosion for stainless steel, carbon steel, copper and alum inum after inmersing in the disinfectant solution containing 0.53% ortho-phthalaldehyde for 72h.The content of ortho-phthalaldehyde declined 2.08% after the product was stored in 37℃ temperature and constant humidity condition for 90 days.The validity duration was at least 2 years.ConclusionThe endoscope disinfection of 0.53% or tho-phthalaldehyde for 5m in could satisfy high level disinfection dem and The disinfectant was stable and ahost no corrosion effect, so it could be used for endoscopic disinfection.
2009, 25(4): 470-471. doi: 10.11847/zgggws2009-25-04-42
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2009, 25(4): 471-472. doi: 10.11847/zgggws2009-25-04-43
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2009, 25(4): 473-474. doi: 10.11847/zgggws2009-25-04-44
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Epidemiological investigation on congenital heart diseases in Hainan region of Qinghai province
WANG Xiao-qin, LIU Feng-yun, GUO Bao-hong
2009, 25(4): 474-475. doi: 10.11847/zgggws2009-25-04-45
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ObjectiveTo investigate the distribution of congenital heart diseases(CHD)in children aged 4-17 in Hainan region of Qinghai province.MethodsHeart auscultation and echocardiography examination on 53827 children from Hainan region of Qinghai province were conducted.ResultsThe overall morbidity of CHD was 5.68‰,with 3.95‰ in males and 7.66‰ in females.Significant difference of prevalence rates was found between different sex(χ2=32.681,P<0.001).Atrial septal defect(32.35%)was noticed as the most common lesion.The next was ventricular septal defects,patent ductus arteriosus and others with significant difference(χ2=40.566,P<0.001).Morbidity rates in different regions were 8.90‰ in Xinghai,7.20‰ in Tongde,5.88‰ in Guinan,5.78‰ in Gonghe,and 3.80‰ in Guide.There was no significant difference in morbidity rates with the increase of altitude.Morbidity rates in different ethnic groups were as follows:6.37‰ in Han,3.70‰ in Mongolian,6.42‰ in Tu,4.05‰ in Tibetan,7.28‰ in Hui.Tibetan had a different morbidity rate compared to Han and Hui(χ2=13.414,P<0.001;χ2=10.904,P<0.005).ConclusionThere were significant regional,sex and ethnic differences in the morbidity of CHD in Hainan region of Qinghai province.
2009, 25(4): 476-477. doi: 10.11847/zgggws2009-25-04-46
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2009, 25(4): 478-478. doi: 10.11847/zgggws2009-25-04-47
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2009, 25(4): 479-479. doi: 10.11847/zgggws2009-25-04-48
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Study on health status and medical care available among Three Gorges migrants
WAMG Jian-wei, GUO Ji-zhi, JIANG Liang-mei
2009, 25(4): 480-481. doi: 10.11847/zgggws2009-25-04-49
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ObjectiveTo study the health status and medical care available among the Three Gorges migrants.MethodsThree hundred and twenty Three Gorges migrants in Shandong were tested with general questionnire.ResultsThe health status of the Three Gorges migrants was lower than that of the local inhabitants.The health status of the subjects was different after the mignition.After the migration, the medical security and medical care available for the Three Gorges migrants was better than before.ConclusionThe mignints have poor health compared with the health status before the migration and more attention should be paid by the government.
Association between daily ambient average temperature and daily respiratory system disease mortality in Shenyang city
WANG Jue, YU Lian-zheng, MU Hui-juan
2009, 25(4): 481-482. doi: 10.11847/zgggws2009-25-04-50
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ObjectiveTo quantitatively evaluate the impact of short-term changes of daily average temperature on residents' daily mortality rate of respiratory diseases in Shenyang city.MethodsAdjusting for long-term trends of air pressure and seasonal factors,the semi-parametric generalized additive model was used to study the relationship between short-time daily average temperature and daily respiratory system disease mortality of the residents from Jan.1,1992 to Dec.31,2000 in Shengyang.ResultsA graphically significant "J" shape relationship between mortality caused by respiratory diseases and ambient temperature was found.The optimal temperature corresponding to low mortality was 22.3℃.The level and length of daily death mortality curve in low temperature(<22.3℃)was higher and longer than that of in high temperature(>22.3℃).The elderly were more sensitive to temperature changing.The mortality caused by COPD(ICD9:490.0-496.9)showed a monotone increase as the temperature decreasing.And the mortality caused by pneumonia(ICD9:480-486)was more sensitive to temperature changing at high temperature.ConclusionThe graphically relationship between ambient daily average temperature and daily respiratory system disease mortality of townsman is a significant "J" shape.
Investigation on epidemic situation of hepatitis B virus and vaccination among children in Jiangsu province
WANG Zhi-guo, MA Fu-bao, TANG Fen-yang
2009, 25(4): 483-485. doi: 10.11847/zgggws2009-25-04-51
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ObjectiveTo analyze the situation of hepatitis B virus(HBV)infection and vaccination and to evaluate the immunization effects after hepatitis B vaccine involved in the expanded program on immunization(EPI).MethodsA survey for 1 894 children aged 1-14 years old was carried out in national surveillance sites using multistage cluster randomized sampling method.Every child's hepatitis B immunization history and main risk factors were investigated through questionnaire.Venous blood samples were collected and tested for HBsAg,anti-HBs and anti-HBc by ELISA method.ResultsAmong the children the rates of hepatitis B vaccine inoculation and timeliness of birth dose in urban area were 97.13% and 78.43%,but the rates in rural area were 95.15% and 57.96%,respectively.The age standardized positive rates of HBsAg,anti-HBs,anti-HBc and HBV infection were 1.10%,70.02%,3.40% and 5.53%,respectively.ConclusionAchievement in hepatitis B control and prevention has been made in Jiangsu province since hepatitis B vaccine was brought into EPI.The positive rate of HBsAg was below 1% for children aged less than 5 years old.The rates of hepatitis B vaccine inoculation and timeliness of birth dose were increased.
2009, 25(4): 485-486. doi: 10.11847/zgggws2009-25-04-52
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Study on prevalence of metabolic syndrome in adults of Jiangxi province
YAN Qu-ru, LIAO Bing-rong, YANG Jian-ping
2009, 25(4): 487-488. doi: 10.11847/zgggws2009-25-04-53
Abstract(1149) HTML (144) PDF 476KB(187)
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ObjectiveTo describe the prevalence of metabolic syndrome(MS)in adults of Jiangxi province,and to provide a scientific basis for MS prevention.MethodsThe MS definition of Chinese Diabetes Association in 2004 and multistage cluster sampling method were used.ResultsThe prevalence of MS was 10.3%.Man had higher prevalence than woman(χ2=4.549,P<0.05).The prevalence of MS increased with age.Apparent increase of the prevalence started at age of 55 in woman.The MS prevalences in different levels of economic area were significantly different(χ2=39.728,P<0.01)with higher MS prevalence rate in high economic level area.ConclusionThe prevalence of MS is high in adults of Jiangxi province.Clustering of MS components is common.
Study on cost and benefit of hepatitis B vaccination in Shenzhen city
SHAN Fu-xiang, LIU Gang
2009, 25(4): 489-490. doi: 10.11847/zgggws2009-25-04-54
Abstract(1259) HTML (210) PDF 778KB(130)
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ObjectiveTo learn the effect and economic benefit of hepatitis B(HB)vaccination and provide basis for middle-long prevention plan for HB.MethodsThe yearly incidence of HB from 1990-2004 and the cost and benefit study were analyzed.ResultsCost benefit study showed that discounted cost was $79 299 043;production benefit was $1 265 454 819 and net benefit was $1 186 155 776.Ratio of cost and benefit was 15.96.Social benefit analysis showed that 25 217 acute HB patients and 12 609 chronic HB patients were prevented from 1993 to 2004.ConclusionHB vaccination has significant economic and social benefit.
2009, 25(4): 490-491. doi: 10.11847/zgggws2009-25-04-55
Abstract(789) HTML (161) PDF 444KB(142)
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Study on burden of diseases among rural people in Dafeng county, Jiangsu province
ZHOU Hua, CHEN Jia-ying, SHA Wen-biao
2009, 25(4): 492-493. doi: 10.11847/zgggws2009-25-04-56
Abstract(925) HTML (109) PDF 253KB(97)
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ObjectiveTo measure the burden of diseases (BOD)among nzral people in Dafeng county with disabilityadjusted life year(DALY) and to identify major health problems and provide evidence for policy malong.MethodsIndirect estimation was used to measure BOD in Dafeng during 1997 and 2006.Time trend of BOD was analyzed.ResultsThe average DALY loss per 1000 people per year was 152.85 in 1997 to 2006.BOD of non-comm unicable diseases (NCD)accounted for 86.08% of total DALYs with 4.72% of communicable diseases and other conditions, and 9.2% of injuries During the ten years, DALY per 1000 people for three major groups of diseases showed a decreasing tendency.Among the first five causes of BOD, psychiatric illnesses, respiratory diseases and unintentional injuries presented significant decreasing trend.The BOD of malignant neoplasms appeared slightly decreasing.However, the BOD of cardiovascular diseases tended to increase.ConclusionHealth status of Dafeng ruralpeople is certainly lower than that of the developed areas in China.NCD such as psychiatric illnesses, malignant neoplasms, and cardiovascular diseases are the major health problems.It is urgent for government to make better community-based health plan and take more effective interventions on NCDs.
Ssurvey on HIV and syphilis infection among drug users in detoxification center in Shenzhen
CHEN Yan-qing, LIN Li-zhu, YU Qi-hui
2009, 25(4): 494-495. doi: 10.11847/zgggws2009-25-04-57
Abstract(1563) HTML (199) PDF 373KB(99)
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ObjectiveTo explore HIV and syphilis infection among drug users in a detoxification center in Shenzhen.MethodsA face to face questionnaire investigation was conducted among 357 drug users, and the subjects were tested for HIV antibody and syphilis RPR.ResultsThe HIV positive rate was 2.24% (8/357) and the syphilis positive rate was 10.08% (36/357) among the subjects There was no statistical difference in HIV positive rate between the male and the fema1e(P=0.9738),but the infection rate for syphilis in women was significantly higher than in men(P<0.0001).There were no statistical difference in positive rate for HIV and syphilis in drug users with different way of drug using(P1= 0.6756, P2=0.8441).In the drug users, the rates of sharing needle and commercial sexual behavior were 20.45%, 15.13%,respectively.Only 11.11% of the subjects with commerial sexual behavior used condom every time.The average age of the drug users was 29.4 years old, and the youngestwas 12 5.88% of the drug users were from local area, the rest were from other places.ConclusionThe age of drug users tends to be younger and most of them are immigrants with high risk behavior of HIV and STD.The management of migmntworkers and health education on STD in adolescents should be enhanced.
Study on antibiotic resistance analysis with microbial source tracking method
YE Xun, GU Ling, LI Wei-wei
2009, 25(4): 495-496. doi: 10.11847/zgggws2009-25-04-58
Abstract(1046) HTML (196) PDF 562KB(120)
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ObjectiveTo set up an antibiotic resistance analysis with microbial source tracking method and track the source of the fecal pollution in a reservoir of the Huaihe River.MethodsFecal samples were collected from known pollution sources around the reservoir and indicator bacteria isolated from the samples were transferred onto the Trypticase Soy A gar plates with different antibiotics.The proper concentrations for the growth of the indicator bacteria were discrin inated with JMP 7.0 sofrivare.Water samples were collected for pollution source tracking.ResultsThe average rates of correct classification from the selected concentrations of antibiotics were 93.05% (two sources),85.75(three sources) and 78.53% (nine sources).The fecal pollution in the water sump les were mainly from swine, cow and chicken with the rate of 36 %,17% and 16%,respectively.ConclusionThe selected concentrations of antibiotics can be used to find the sources of the indicator bacteria.Antibiotic resistance analysis with microbial source tracking method can be applied to track the source of water bacterial pollution.
Survey on knowledge, attitude of anti-earthquake and disaster mitigation among residents of disaster area
ZHENG Shan, CHEN Yong-cong, WANG Yu
2009, 25(4): 497-498. doi: 10.11847/zgggws2009-25-04-59
Abstract(964) HTML (169) PDF 296KB(78)
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ObjectiveTo provide a way for restoration and reconstruction of disaster area through surveying the knowledge and attitude of disaster resistance among residents of disaster area.MethodsA face-to-face interview was conducted among the urban and rural residents in Kangxian county to collect data on their knowledge and attitude of disaster resistance.ResultsOf all the investigated people,610 people(65.4%)didn't know how to self-rescue after the earthquake,which had a significant difference between urban and rural residents(P=0.000).258 people(27.7%)didn't know there was likely to appear epidemic of infectious diseases after earthquake.Over 80.0% of interviewees thought they were satisfied with the efficiency of emergency actions and health services provided by local government.703 people(75.3%)believed that capital scarcity was the major hamper for the reconstruction of their home.505 people(54.1%)had full confidence to reconstruct home.And mass media were found to be the main way for the residents to acquire the disaster knowledge.ConclusionThe cognition rate of anti earthquake and disaster mitigation among the residents of Kangxian county is low,so it is very necessary for people to improve their knowledge and skills of disaster resistance,and meanwile for government to improve and complete the operational capability and system against the emergent natural disaster.
2009, 25(4): 499-500. doi: 10.11847/zgggws2009-25-04-60
Abstract(1150) HTML (317) PDF 386KB(180)
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Effect of iron deficiency duing pregnancy on fetus development in rat
LIU Min, MA Ya-jing, CHENG Jiang
2009, 25(4): 500-501. doi: 10.11847/zgggws2009-25-04-61
Abstract(991) HTML (209) PDF 380KB(94)
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ObjectiveTo establish a nutritional iron deficiency model in pregnancy rats and to study the effect of iron deficiency anemia on fetus development.MethodsSix-month healthy female Wistar rats were randomly divided into two groups.The rats in experimental group and control group were fed with low iron or normal diet,respectively.After four weeks,the rats were mated with healthy adult male rats.Before the mating,levels of hemoglobin,erythrocyte hemoglobin distribution width,ferritin were detected.After the offspring were born,the conception rate,the development and growth of the newborn until weaning were observed.ResultsHemoglobin and weight were lower in experimental group than those in control group(P<0.05)before the mating.Erythrocyte hemoglobin distribution width and free erythrocyte protoporphyrin were higher in experimental group.The conception rates of two groups were the same(83.3%).There were significant differences in offspring survival rate,birth rate,and growth rate between the two groups(P<0.05).ConclusionThe nutritional iron deficient model in pregnancy rats was established successfully.Pregnant rats iron deficiency anemia can affect their fetus growth.
2009, 25(4): 502-503. doi: 10.11847/zgggws2009-25-04-62
Abstract(905) HTML (157) PDF 361KB(104)
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Effect of sleep restriction on concentration and memory diversification
WU Hui-li, CHEN Gang, LU Xue-liang
2009, 25(4): 503-504. doi: 10.11847/zgggws2009-25-04-63
Abstract(899) HTML (242) PDF 366KB(171)
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ObjectiveTo evaluate the changes of the human memory and concentration after two nights of sleep restriction.MethodsTwelve tests(6 memory tests and 6 typing tests)were conducted in two groups of medical students(n=13),druing the 2 nights of sleep restriction.Scores of the memory game and quantity of the typing test were used for the memory and concentration evaluation.Coupling t analyses on the results of last 5 tests with that of first were conducted.ResultsThere was significant difference in tests in tests'score between first test and the last 5 tests in A group(P<0.05).There was no significant difference in group B(P>0.05).ConclusionMemory game and typing test can be used to evaluate the changes of human memory and concentration after two nights of sleep restriction.
2009, 25(4): 505-505. doi: 10.11847/zgggws2009-25-04-64
Abstract(1010) HTML (151) PDF 134KB(112)
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2009, 25(4): 506-506. doi: 10.11847/zgggws2009-25-04-65
Abstract(909) HTML (186) PDF 171KB(74)
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2009, 25(4): 507-508. doi: 10.11847/zgggws2009-25-04-66
Abstract(1026) HTML (130) PDF 410KB(108)
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2009, 25(4): 509-511. doi: 10.11847/zgggws2009-25-04-67
Abstract(787) HTML (149) PDF 220KB(116)
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Effects of exercise on iron regulatory protein-2 gene expression in brain aging mice
YUN Shao-jun, HE Xiao-juan, ZHANG Shu-hua
2009, 25(4): 511-512. doi: 10.11847/zgggws2009-25-04-68
Abstract(906) HTML (123) PDF 431KB(101)
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ObjectiveTo explore the effects of exercise on the expression of iron regulatory protein-2(IRP-2)gene in brain aging mice.MethodsForty mice were randomly divided into four groups:control group,exercise group,brain aging group,brain aging+exercise group.The experimental duration was 10 weeks.Morris water maze test was used to measure the learning and memory ability.Reverse transcription polymerase chain reaction(RT-PCR)was used to detect the brain IRP-2 gene expression.ResultsIRP-2 gene expression decreased in brain aging mice(0.738±0.221)and reached to the extent of statistical significance(P<0.05).There was no difference in IRP-2 gene expression between exercise group(2.595±0.822)and control group(3.925±2.357).Compared with the control group,there was no remarkable effect in brain aging+exercise group(2.938±0.468),but the IRP-2 gene expression in brain aging+exercise group was higher than that in brain aging group(P<0.05).ConclusionExercise can prevent the decreasing of IRP-2 gene expression in brain aging mice.