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2015 Vol. 31, No. 3

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Contraceptive choices among married child-bearing women in China
TIAN Xiao-bo, BAI Fu, LI Bo-hua.et al
2015, 31(3): 257-259. doi: 10.11847/zgggws2015-31-03-01
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ObjectiveTo investigate contraceptive knowledge and choices among married child-bearing women in China.MethodsWe conducted this survey during October 2010 in 30 counties.Population proportional to size sampling method was employed in sampling.A total of 6 753 married child-bearing women were interviewed face-to-face.Information on demographic characteristics,contraceptive knowledge and choices were obtained.ResultsThere was a high awareness rate of oral contraceptive pills,condom,intrauterine device and female sterilization,but a relatively low awareness rate of other contraceptive methods.Among the 6 568 women who knew the condom,over 1/4 believed the condom could took off after the penis being flaccid;over 30% believed air leak checking by blowing the condom need to be conducted before using or oily lubricant could be used;89.1% reported experiencing at least one contraceptive method and 82.7% using at least one contraceptive method during the period before the survey and commonly used contraceptive methods reported by the women were intrauterine device(56.7%),female sterilization(21.0%),and condom(18.5%).ConclusionThe contraceptive rate is high but the awareness and using rate of contraceptive methods other than intrauterine device,female sterilization and condom are relatively low among married child-bearing women in China,and some of the women have misunderstandings on condom use.
Utilization of gynecological examination service and its related factors among floating women
ZHU Lin, LIU Hong-yan, WU Ji-lei.et al
2015, 31(3): 260-263. doi: 10.11847/zgggws2015-31-03-02
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ObjectiveTo examine the utilization of health care service and its related factors among floating women of childbearing age and to provide evidences for improving the quality of reproductive health for the women.MethodsWith the data from the reproductive health survey in floating population conducted in Beijing,Nanjing,Xiamen,Shen-zhen,and Chongqing municipality in 2005,the status of gynecological examination(GE)among 5 399 floating women was observed.Multivariate logistic regression was performed to analyze the factors related to the utilization of GE.ResultsThe results of regression showed that the proportion of having GE among married women and women with other marital status(widowed and divorced)was 7.46-fold(95% confidence interval[95%CI]=5.45-10.21)and 4.86-fold(95%CI=2.33-10.15)higher than that among unmarried women.The proportion of having GE in the women with one parity was 1.47-fold(95%CI=1.13-1.90)higher than that in the women without childbearing history.The proportion of having GE in the women with the certificate of marriage and fertility for floating population was 1.41-fold(95%CI=1.24-1.61)higher than that in the women without the certificate.The proportion of having GE in the women who had signed family planning management agreement for floating population was 1.33-fold(95%CI=1.17-1.52)higher than that in the women did not signed the agreement.The proportion of having GE in the women participating in the education program organized by family planning departments was 1.56-fold(95%CI=1.38-1.76)higher than that in the women not participating in the program.The proportion of having GE in the women with self-reported normal or poor health condition was 1.25-folder(95%CI=1.08-1.44)and 1.80-folder(95%CI=1.25-2.60)higher than that in the women with self-reported good health condition.ConclusionMarried or ever married,having less parities,with certificate of marriage and fertility for floating people,with family planning management agreement for floating population,taking education programs organized by family planning departments,and with self-reported poor health are protective factors for utilization of GE in floating childbearing women.
Psychological resilience and its influencing factors among breast cancer patients
ZHANG Ting, LI Hui-ping, WANG De-bin
2015, 31(3): 263-267. doi: 10.11847/zgggws2015-31-03-03
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ObjectiveTo investigate psychological resilience and its influencing factors among breast cancer patients.MethodsTotally 381 hospitalized breast cancer patients were recruited from 2 grade 3 hospitals in Anhui province and investigated with General Information Questionnaire,Resilience Scale(RS-14),Perceived Social Support Scale(PSSS),Self-Esteem Scale(SES),General Self-Efficacy Scale(GSES),Revised Life Orientation Test(LOT-R),Health-Related Hardiness Scale(HRHS),and Mastery Scale for Chinese version(MSC)from October 2013 through May 2014.ResultsThe average score of the participants was 2.68±0.46 for self-efficacy,21.35±3.40 for self-mastery,15.23±3.98 for self-esteem,17.45±3.23 for optimistic tendency,73.50±7.89 for social support,103.73±9.00 for hardiness personality,and 76.82±10.90 for resilience,respectively.The level of psychological resilience among the patients belongs to superior middling,with an average overall score of 5.49±0.78.There existed a difference in RS-14 scores among the patients with different living residence,education level,status of employment,form of payment,and disease cognition(all P<0.01).Univariate analysis indicated that the RS-14 scores were positively correlated with scores of SES,GSES,PSSS,LOT-R,HRHS and MSC(r=0.105-0.608, all P<0.01).The results of multivariate linear regression analysis showed that disease cognition,form of payment,optimism,social support,self-efficacy,and self-mastery were main influencing factors of psychological resilience among the patients.ConclusionThe level of psychological resilience should be enhanced among breast cancer patients and disease cognition,form of payment,optimism,social support self-efficacy,and self-mastery are related to psychological resilience among breast cancer patients.
Factors associated with antenatal care service utilization among women of childbearing age in rural areas of Shaanxi province:a structural equation model analysis
ZHU Zhong-hai, CHENG Yue, LIU Dan-li.et al
2015, 31(3): 268-272. doi: 10.11847/zgggws2015-31-03-04
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ObjectiveTo study current status and direct and indirect influencing factors of antenatal care service utilization among women of childbearing age in rural areas of Shaanxi province,and to provide evidences for making policy on health care of the women.MethodsA population-based cross-sectional survey was conducted with a questionnaire survey about health care of the women during perinatal period in rural areas of Shaanxi province.Stratified multistage random sampling was adopted to obtain a sample of 4 779 women giving birth to their children during 2007-2009.Confirmatory factor analysis was used to construct measure models.Parameters and final model were fitted and revised by generalized weighted least square technique.ResultsOf all the women,63.5% had their first antenatal care check within 12 gestation weeks;43.8% received 5 times or more antenatal care checks;30.2% of them completed all required examinations;89.3% attended township hospitals or higher level health facilities for their antenatal care checks;but only 14.2% had qualified antenatal care checks.Structural equation modeling analysis showed that family characteristics,women's social-demographic characteristics,and health knowledge had effect on antenatal care utilization,with the effectiveness percentages of 62.1%,24.4%,and 12.5%,respectively.ConclusionThe quality of antenatal care for pregnant women living in rural areas of Shaanxi province needs to be improved.Health promotion on antenatal care in these areas should target on childbearing women who are from poor families,with lower educational level,and at elder age.
Effect of pre-pregnancy BMI and parity on hypertensive disorders among pregnant women
ZHU Ruo-ping, HAO Jia-hu, ZHU Peng.et al
2015, 31(3): 272-275. doi: 10.11847/zgggws2015-31-03-05
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ObjectiveTo explore whether adverse effects of pre-pregnancy body mass index(BMI)on hypertension disorders in pregnancy are moderated by gravidity.MethodsA total of 1 633 pregnant women who had antenatal care in their early pregnancy and delivered from February 2011 to December 2012 were selected as study subjects.The associations of parity and pre-pregnancy BMI with hypertension disorders in pregnancy were evaluated with Chi-square analysis and logistic regression.ResultsThe average pre-pregnancy BMI for the pregnant women was 20.20(2.418 kg/m2).Based on the pre-pregnancy BMI,367 women were classified as emaciation(22.5%),1 153 as normal(70.6%),and 113 as overweight or obese(6.9%).Of the women, 936(57.3%)were nulliparous and 697(42.7%)were multiparity.Univariate analysis showed that the risk of hypertension disorders among pre-pregnancy overweight or obese pregnant women increased with the odds ratio(OR)of 2.722(95% confidence interval[95%CI]=1.074-6.900,P=0.035)and 4.356(95%CI=1.865-10.171,P=0.001).After adjusting for confounding factors such as pregnant chronological age,education level,and job category,multivariate analysis showed that parity was a risk factor of hypertension disorders in pregnancy for pre-pregnancy overweight or obese pregnant women(for multiparity:OR=3.535,95%CI=1.472-8.491;P=0.005).ConclusionPre-pregnancy overweight or obese pregnant women who had more parity are at a higher risk of hypertensive disorders in pregnancy.
Risk factors of stroke among urban community residents aged 50 years and older in Shanghai:a cross-sectional study
ZHU Wei-hong, HUANG Jiu-yi, GUAN Yang-tai
2015, 31(3): 276-279. doi: 10.11847/zgggws2015-31-03-06
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ObjectiveTo describe risk factors of stroke and the relationship between cerebral hemodynamic index(CVHI)abnormality and stroke risk among urban community residents aged ≥50 years in Shanghai and to provide references for stroke intervention.MethodsA questionnaire survey,physical examination and CVHI measurement were conducted among 2 031 residents at the age of 50 years or older selected with cluster sampling from Changbai and Wujiaochang communities of urban Shanghai.ResultsAmong the participants,the prevalence rates for conventional risk factors of stroke were 51.1% for hypertension,30.3% for cardiovascular disease,18.2% for diabetes mellitus,21.6% for hyperlipidemia,26.9% for familial stroke history,46.3% for overweight or obesity,11.6% for smoking,and 8.0% for alcohol consumption,respectively.There were 42.0% of the participants were at a high risk of stroke(with the integral CVHI score of<75)and the percentage of the individuals with the CVHI score of<75 increased significantly with the increment of age among the participants(P<0.01).ConclusionThe prevalence of stroke risk factors is at a high level and the number of persons at a higher stoke risk increases with the increment of age among urban community residents at their age of 50s and over in Shanghai.
Attitude towards premarital sex and its influence factors among unmarried females in Luzhou city
XIONG Wei, YE Yun-li, LEI Zhang-heng.et al
2015, 31(3): 279-281. doi: 10.11847/zgggws2015-31-03-07
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ObjectiveTo explore the attitude towards premarital sex and its influence factors among unmarried females in Luzhou city,Sichuan province,and to provide scientific evidences for reproductive health education.MethodsWith multi-stage random sampling,a self-administered anonymous questionnaire survey was conducted among 2 119 unmarried females aged ≥16 years in Luzhou city.ResultsOf 1 989 participants with valid information,59.83% were not against premarital sex and 24.73% reported premarital sex experience. Multivariate logistic regression analysis showed that the participants at the age of 20-24 and ≥25 years were less likely to oppose premarital sex than those aged ≤20 years old,with the odds ratios(OR)of 0.336(95% confidence interval[95%CI]=0.211-0.537)and 0.460(95%CI=0.288-0.737);compared with those never receiving sexual education,the participants receiving sexual education frequently and occasionally were less likely to oppose premarital sex,with the OR of 0.472(95%CI=0.289-0.770)and 0.622(95%CI=0.408-0.950);the participants with the knowledge about safe period for contraception were less likely to oppose premarital sex than those without the knowledge,with the OR of 0.611(95%CI=0.472-0.789);the participants having their first menstruation at the age of 13-15 years and ≥ 16 years were more likely to oppose premarital sex than those at the age 10-12 years,with the OR of 2.794(95%CI=1.313-5.949)and 1.636(95%CI=0.783-3.419).ConclusionAge,condition of receiving sexual education,whether having the knowledge about safe period for contraception,and the age at first menstruation are influence factors of attitude towards premarital sex among unmarried females in Luzhou city.
Prevalence and influencing factors of premarital pregnancy among married women of child-bearing age
JIAN Min-ting, LI Xiao-ning, ZHOU Shu-dong.et al
2015, 31(3): 282-284. doi: 10.11847/zgggws2015-31-03-08
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ObjectiveTo examine the prevalence of premarital pregnancy among married women of child-bearing age and analyze its influential factors to provide evidences for the improvement of reproductive health in the women.MethodsStratified cluster sampling was applied to select 937 local and 358 migrant married women of child-bearing age in Xiqiao town of Foshan city for a questionnaire survey.ResultsAmong all the women,the rate of premarital pregnancy was 32.43%.Among the 420 women with premarital pregnancy history,48(11.43%)gave births to their children; 262(62.38%)married because of the premarital pregnancy; 60(14.29%)had abortion; 15(3.57%)had both induced abortion and giving the birth to the children; and 35(8.33%)had induced abortion and got married because of the premarital pregnancy.The premarital pregnant rate was higher among floating women(37.43%),the women aged 20-29 years(46.95%)and 30-39 years(34.03%),and the women with junior high school education(34.81%) and senior high school or vcational school education(38.93%),with the odds ratio of 1.634,4.711,2.447,2.487,and 2.144,respectively.ConclusionThe rate of premarital pregnancy is high among the married women of child-bearing age in Foshan city,suggesting health education on reproductive health needs to be promoted among the women,especially in migrant and young married women at child-bearing age.
Physical activity and its influencing factors among working pregnant women in Minhang district of Shanghai city
ZHOU Re-na, YU Feng, HUANG Xiao-xia.et al
2015, 31(3): 285-287. doi: 10.11847/zgggws2015-31-03-09
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ObjectiveTo examine the status and influencing factors of physical activity(PA)among working pregnant women in Minhang district of Shanghai city.MethodsA questionnaire-based survey was conducted among 2 170 pregnant women residing in Shanghai≥6 months and with stable employment during the pregnancy from May 2011 through May 2012.ResultsThe most prevalent PA among the working pregnant women was transportational PA but only 26.77% of the women participating transportational PA and the ratio of the women taking working PA,transportational PA,and leisure-time PA actively were 4.38%,9.26%,and 1.11%,respectively.The average daily sedentary time of the women was about 7 hours.Among the women,age was positively associated with the participation of leisure-time moderate physical activity(MPA),while negatively with that of working MPA;the participation of transportational PA was positively associated with age,but negatively with gestational weeks and family economic level;and the women being primipara were more likely to take transportational PA,but not MPA during working.ConclusionThe overall PA was at a low level,with a relatively higher level of transportational PA,and PA during pregnancy was associated with age,gestational weeks,family economic level,and whether being primipara among the working pregnant women in Shanghai.
Effect of evidence-based nursing on labor outcomes among puerperae with natural childbirth in China:a meta-analysis
CHENG Li-nan, CUI Wen-xiang, CUI Ying-shan
2015, 31(3): 288-291. doi: 10.11847/zgggws2015-31-03-10
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ObjectiveTo systematically assess the effect of evidence-based nursing on labor outcomes among Chinese puerperae with natural childbirth and to provide evidences for clinic nursing.MethodsRelevant literatures were searched through Chinese databases such as Chinese National Knowledge Infrastructure(CNKI),Wanfang,and Weipu via internet according to the inclusion and the exclusion criteria.Note Express software was used in screening and duplicate checking of the literatures and RevMan 5.2 software was adopted in the analyses.ResultsA total of 14 studies were included in the study,with totally 2 028 participants in the intervention group and 1 984 in the control group.The results of meta-analysis showed that evidence-based nursing could shorten the time(in hour) of first stage of labor,with a mean difference(MD)of-2.3 and its 95% confidence interval(95%CI)of -2.60--2.01),the time of second stage of labor(MD=-0.41,95%CI:-0.54--0.28),and the time of third stage of labor(MD=-0.05,95%CI:-0.05--0.04).Moreover,the implementation of evidence-based nursing could significantly decrease the rate of neonatal asphyxia,with an odds ratio of 0.36(95%CI=0.27-0.4). ConclusionApplication of evidence-based nursing can shorten parturient labor time of natural childbirth and reduce the incidence of neonatal asphyxia and its clinical application should be promoted.
Long-term change trend in physique development among Tujia and Miao children and adolescents,1985-2010
ZHANG Tian-cheng, CAI Chen, ZHANG Fu-lan.et al
2015, 31(3): 292-295. doi: 10.11847/zgggws2015-31-03-11
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ObjectiveTo explore long-term change trend in physique development among Tujia and Miao children and adolescents during the period of 1985-2010 and to provide bases for improving and enhancing the growth and development of minority children and adolescents in destitute areas.MethodsThe data on height and weight of Tujia and Miao school students aged 7-18 years were collected from three cross-sectional surveys on physique and health among Chinese students conducted in 1985,2000,and 2010.Body mass index(BMI),maximum increment age in height(MIA-H),and maximum increment age in weight(MIA-W)of the Tujia and Miao students at different years were calculated and analyzed.ResultsDuring the period of 25 years,the average height increment of the students at the age of 7 to 18 years was 3.38 cm and 3.22 cm for Tujia males and females and 3.88 cm and 3.60 cm for Miao males and females;the average weight increment was 4.12 kg and 2.52 kg for Tujia males and females and 3.17 kg and 2.62 kg for Miao males and females;the average BMI increment was 1.22 kg/m2 and 0.55 kg/m2 for Tujia males and females and 0.71 kg/m2 and 0.61 kg/m2 for Miao males and females,respectively.A trend of slowing at younger age and accelerating at elder age in growth speed and a trend of sturdy in physique development were observed among the Tujia students;whereas a trend of accelerating at younger age and slowing at elder age in growth speed and a trend of slender body in physique development were observed among the Miao students.The key population for overweight and obese surveillance among the children and adolescents was the students at the age of 10-13 years.For all the students,the MIA-H and MIA-W increased by 0.40-3.98 years,except for the Miao males.A minor acceleration in height increment was observed in the students aged 18 years,with a gender difference of less than 13 cm in height increment.ConclusionThere is a long-term increasing trend in physique development among Tujia and Miao children and adolescents,with a rapid increasing stage for the Tujia and an initial stage for the Miao;there are also ethnic and gender differences in the long-term increasing trend among Tujia and Miao children and adolescents.
Public opinions on governmental measures for H7N9 avian influenza control and prevention:a survey through microblog
ZHANG Xiao-hua, HOU Qian, WANG Hui.et al
2015, 31(3): 296-298. doi: 10.11847/zgggws2015-31-03-12
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ObjectiveTo study the public satisfaction to governmental measures for H7N9 avian influenza control and prevention.MethodsSystematic sampling was used to select participants among microblog users of 12320 Health Hotline via Sina and Tencent service for a questionnaire survey.ResultsOf the 1 166 participants with valid response,67.2% were satisfied with the measures for H7N9 avian influenza prevention and control taken by the government in the previous one month;79.8% considered the dissemination of the epidemic information being timely;93.4% were satisfied with the knowledge provided by the microblog account of 12320 Health Hotline;81.1% regarded the government having the capability to control the epidemic completely;and 44.5% reported the changes in their health habits after the epidemic.The participants at elder age were more satisfied with governmental measures to prevent and control the epidemic and the timely dissemination of epidemic information compared to the participants at younger age.The results of logistic regression showed that the participants living in the epidemic area,worrying about being infected by the virus,and regarding the government having the capability to control the epidemic were more likely to change their health habits related to the epidemic compared to those living in non-epidemic area,worrying about the virus infection a little(not worrying at all),and not considering the government having the capability.ConclusionThe governmental information dissemination and knowledge promotion for H7N9 avian influenza epidemic are effective and the public is satisfied with the information on the epidemic control and prevention provided by the microblog account of 12320 Health Hotline.
Characteristics of smoking intensity and inhalation among urban and rural cigarette smokers in Liaoning province
YU Li-ya, LI Yan-xia, LIU Li.et al
2015, 31(3): 299-302. doi: 10.11847/zgggws2015-31-03-13
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ObjectiveTo analyze the characteristics of detailed smoking behavior among urban and rural cigarette smokers in Liaoning province and to provide evidences for smoking control.MethodsThe data of 2 771 smokers were extracted form a stratified random cluster sample of 8 612 urban and rural residents involved in a questionnaire survey on risk factors of non-communicable chronic diseases in Liaoning province in 2009;the length of a cigarette rod smoked and the depth of smoke inhalation during smoking among the smokers were analyzed.ResultsThe prevalence of smoking among the residents was 32.2% and the smoking prevalence of the male residents was significantly higher than that of the female(53.7% vs.11.6% ;χ2=1 746.708,P<0.01).Of the smokers,22.63%,33.63%,and 43.74% reported the number of cigarette smoked per day of <10,10-19,and ≥20;the proportion of the smokers reporting the length of cigarette rod smoked once of <1/2,1/2-3/4,and all of a cigarette were 7.49%,27.76%,and 64.75% ;and the proportion of the smokers reporting almost no inhalation,slight,moderate,and deep inhalation were 8.47%,21.25%,35.41%,and 34.87%,respectively.Compared to the female smokers,the male smokers had higher proportions of consuming cigarettes ≥20 per day(45.99% vs.33.79%),with 1/2-3/4 length of cigarette rod smoke once(28.77% vs.23.33%),and inhaling a lot of cigarette smoke(36.59% vs.27.25%)(all P<0.01).Correlation analysis showed that the amount of cigarette smoked per day associated positively with cigarette rod length smoked once(r=0.192,P<0.01)and inhalation depth of cigarette smoke(r=0.234,P<0.01),and the cigarette rod length smoked once was positively correlated with the inhalation depth of cigarette smoke(r=0.339,P<0.01).ConclusionThere are significant differences in cigarette rod length smoked once and cigarette smoke inhalation depth among the smokers of different gender and smoking intensity and interventions on smoking behaviors could reduce smoking-related health risk among smokers in Liaoning province.
Smoking prevalence and its change trend among adults in Xuecheng district of Zaozhuang city
ZHANG Yi-cheng, ZHOU Cheng-chao, GUO Xiao-lei.et al
2015, 31(3): 303-305. doi: 10.11847/zgggws2015-31-03-14
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ObjectiveTo examine the change trend of smoking among adults in Xuecheng district of Zaozhuang city from 2004 to 2010 and to provide references for effective tobacco control.MethodsFace-to-face questionnaire surveys on smoking behaviors were carried out among 420,320,and 660 urban or rural residents aged ≥ 18 years selected with stratified cluster random sampling in 2004,2007,and 2010 in Xuecheng district of Zaozhuang city,Shandong province.Multivariate logistic regression was used in data analyses.ResultsThe smoking rate was 17.86%,29.06%,and 25.30% and the passive smoking rate was 34.52%,61.88%,and 11.97% among the participants in 2004,2007,and 2010,with significant between-year differences in both the smoking and passive smoking rate(all P<0.05).For the smokers among the participants,the age at starting smoking was 23.95(standard deviation:±8.12),22.17±5.70,and 22.45±5.47 years and the average number of cigarette consumed per day were 18.68±13.75,21.30±12.58,and 19.73±10.84 in 2004,2007,and 2010,without significant between-year differences in the age and the amount of cigarette smoking(all P>0.05).The results of multivariate logistic regression showed that gender and age were influencing factors of current smoking rate among the participants.ConclusionSmoking rate,passive smoking rate,and the number of cigarette consumed per day by the smokers are at a higher level and the smoking rate rises with the increment of age among the adults in Xuecheng district of Zaozhuang city.
Establishment of immortal cell lines from Shui and Gelao ethnic populations in Guizhou province
LI Ming, ZHANG Guang-chao, SUN Liang-xian.et al
2015, 31(3): 306-308. doi: 10.11847/zgggws2015-31-03-15
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ObjectiveTo establish an immortal cell line from Shui and Gelao ethnic populations in Guizhou province and to provide a permanent research material for life science research.MethodsTotally 144 peripheral venous blood samples from Shui ethnic people in Sandu county and 124 from Gelao in Wuchuan county in Guizhou province were collected.B lymphocytes isolated from the blood samples were transformed using Epstein-Barr(EB) virus infection and cyclsoporine A(CyA) treatment.The morphology of the transformed cells was observed and direct counting method was used to make cell growth curve and to calculate cell doubling time of the transformed cells.Passage,frozen storage,and recovery of the transformed cells were performed and culture was conducted to detect mycoplasma contamination.ResultsTotally 138 immoral B lymphocyte cell lines from Shui ethnic group and 115 from Gelao ethnic group were successfully established with the conversion rates of 95.8% and 92.7%,respectively.The transfored B lymphocytes present typical cell deformation,cloning growth characteristics,and normal growth after recovery,without mycoplasma contimination.ConclusionPeripheral B lymphocytes from Shui and Gelao ethnic populations were transformed into immortal cell lines successfully for further researches.
Protective effect of ginsenoside Rg1 on immune-mediated liver injury in mice
YANG Yi, QIN Xiao-yan, GUO Zhe.et al
2015, 31(3): 309-311. doi: 10.11847/zgggws2015-31-03-16
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ObjectiveTo explore protective effects and mechanism of ginsenoside Rg1(Rg1) on immune-mediated liver injury in mice.MethodsHigh,medium and low Rg1 dose(60,30,15 mg/kg) group were established by gavage once a day for 15 days and mice liver injury model was established by tail vein injection of 20 mg/kg concanavalin A(Con A).Serum alanine transaminase(ALT) and aspartate aminotransferase(AST) of the mice were examined by using automatic biochemical analyzer.Serum interferon gamma(IFN-γ) and tumor necrosis factor alpha(TNF-α) were determined with enzyme-linked immunosorbent assay(ELISA),and liver tissue was histopathologically examined with hematoxylin-exsin(HE)staining.ResultsCompared with the control group,serum AST(235.5±79.9 U/L),ALT(262.1±63.9 U/L),TNF-α(46.3±13.3 pg/mL),and IFN-γ(165.3±86.1 pg/mL)were significantly increased in model group(P< 0.01 for all).Compared with the model group,serum AST(57.1±19.9 U/L)ALT(44.1±16.2 U/L),TNF-α(12.9±6.1 pg/mL),and IFN-γ(55.06±29.5 pg/mL)were obviously decreased(P<0.01 for all)in high dose Rg1 treatment group.Histological observation showed that the degree of liver injury in the mice of each Rg1 dose group was obviously lighter than that of the model group.ConclusionRg1 has certain protective effect on immune-mediated liver injury and its mechanism may be related to the reduction of inflammatory cytokines and toxic effect of T lymphocytes.
Establishment of an oxidative damage model induced by hydrogen peroxid in TM3 cells
ZHAO Jian, REN Shi, ZHAI Ling-ling.et al
2015, 31(3): 312-314. doi: 10.11847/zgggws2015-31-03-17
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ObjectiveTo establish an oxidative damage model with hydrogen peroxide(H2O2)in mouse Leydig(TM3)cells for studying possible mechanism of decreased reproductive function in obese males.MethodsAfter 4 hours' treatment with different concentration of H2O2, the cell viability was determined with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT)method;the testosterone level was determined with enzyme-linked immunosorbent assay(ELISA);the contents of reactive oxygen species(ROS),total antioxidant capacity(TAOC)and activities of superoxide dismutase(SOD) and catalase(CAT)were detected with spectrophotometry.ResultsThe viability of TM3 cells (86.7±4.3% and 46.3±2.0%)of 300 and 600 μmol/L H2O2 exposure groups decreased significantly compared to that of the control group(100±1.7%)(all P<0.05).The testosterone level(188.93±7.06,179.23±6.66,and 131.59±11.26 pg/mL)of 150,300,600 μmol/L H2O2 exposure groups were significantly lower than that of the control group(250.09±26.70 pg/mL)(all P<0.05).The activity of SOD(2.42±0.17,2.69±0.30,and 0.44±0.41 U/mg protein)and CAT(56.76±12.17,23.45±2.17,and 24.55±18.05 U/mg protein)of 150,300,and 600 μmol/L H2O2 exposure groups decreased significantly compared to those of the control group(all P<0.05).ConclusionThe TM3 cell model of oxidative injury was established successfully with the administration of H2O2.
Formononetin-induced apoptosis in bladder cancer cells and its mechanism
ZHANG Xing, LIANG Mei-hua, HUANG Wen-jun.et al
2015, 31(3): 314-317. doi: 10.11847/zgggws2015-31-03-18
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ObjectiveTo explore the underlying mechanism responsible for formononetin-induced apoptosis of bladder cancer cells in vitro.MethodsHuman bladder cancer cells T-24 and BIU-87 were exposed to different doses of formononetin(20,40,and 80 μmol/L).Thiazolyl blue tetrazolium bromide(MTT)assay was applied to determine the effect of formononetin on proliferation of T-24 and BIU-87 cells;the apoptosis was assessed with flow cytometry and Hoechst 33258 staining.Next,the expression level of Bcl-2 mRNA in T-24 cells was analyzed with real-time reverse transcription polymerase chain reaction(RT-PCR).The expressions of p-p38 and Bcl-2 proteins in T-24 cells were determined with Western blot.ResultsCompared with the control group,the formononetin at the doses of 40 and 80 μmol/L significantly suppressed the proliferation of T-24 and BIU-87 cells,while the apoptotic rate increased significantly.The Hoechst 33258 staining results showed that T-24 cells exhibited typical apoptotic morphology.Compared with the control group,formononetin effectively reduced the expression of Bcl-2 mRNA(0.701±0.029 for 40 μmol/L group and 0.408±0.036 for 80 μmol/L group)in T-24 cells(all P<0.01).Compared with the control group,formononetin effectively increased the expression of P38 protein(0.483±0.026 for 20 μmol/L group and 0.569±0.031 for 80 μmol/L group)in T-24 cells(all P<0.01).Compared with the control group,formononetin effectively reduced expression of Bcl-2 protein(0.998±0.034 for 20 μmol/L group,0.591±0.030 for 40 μmol/L group,and 0.426±0.027 for 80 μmol/L group)in T-24 cells(all P<0.01).ConclusionFormononetin could inhibit the growth of bladder cancer cells by induction of apoptosis,which may be related to the inhibition of Bcl-2 expression and activation of P38 protein.
Impact of equol on apoptosis and proliferation of vascular endothelial cells
JIN Lian-hai, ZHU Jie, ZHANG Chen-hao.et al
2015, 31(3): 318-320. doi: 10.11847/zgggws2015-31-03-19
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ObjectiveTo study the effect of equol(Eq),a metabolite of soybean isoflavones,on apoptosis and proliferation of vascular endothelial cells.MethodsVascular endothelial cells(ECV-304)were cultured in vitro and treated with different concentrations of Eq (0, 6.25,12.5,25,50,and 100 μmol/L) for 24 hours and then the cells were collected.Reactive oxygen species(ROS),apoptosis and cell cycle were determined with flow cytometry.Apoptosis-related proteins(Bax,Bcl-2,and caspase-3)were detected with immunohistochemical method.Eq-related cell proliferation inhibition rate(IR)was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)reduction assay.ResultsCompared with that of the control group,lower concentration of Eq(6.25 and 12.5 μmol/L)reduced intracellular ROS,whereas the higher concentration(100 μmol/L)of Eq increased intracellular ROS.The apoptosis rates of the cells treated with Eq of higher concentration(25,50 and 100 μmol/L)were increased(9.67%,12.17%,and 12.87%).Lower concentration of Ep did not affect the cell cycle also,while higher concentration of Ep caused G1 arrest.The IR of the ECV-304 cells treated with different concentrations of Eq(6.25,12.5,25,50,and 100 μmol/L) were -8.23±2.21%,-3.29±1.07%,6.75±1.73%,25.46.23±4.82%,and 36.93±4.66%,respectively.Lower concentration of Eq promoted but the higher concentration inhibited the proliferation of the cells.Compared with the control group,the expression of Bax and caspase-3 in the cells treated with Eq(25 μmol/L)for 24 hours increased,while the expression of Bcl-2 decreased.ConclusionLower concentration of Eq could reduce intracellular ROS,promote cell proliferation,while higher concentration of Eq could induce cell apoptosis and cell cycle arrest.
Effects of lignans on spermatogenesis in mice exposed to lead acetate
SHEN Nan, WANG Yan-chun, FAN Hong-yan.et al
2015, 31(3): 321-323. doi: 10.11847/zgggws2015-31-03-20
Abstract(1034) HTML (226) PDF 1031KB(92)
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ObjectiveTo study effects of lignan on spermatogenesis and its mechanism in mice with lead acetate exposure.MethodsSixty Kunming male mice were randomly divided into a control group,model group,positive drug group,and lignans group.Except for those in the control group,all the mice were injected lead acetate for 10 days at the dosage of 40 mg·kg-1·d-1 to establish spermatogenesis disorder model; two days after lead acetate injection,the mice in positive drug group were treated with human chorionic gonadotropin(500 IU·kg-1·d-1) via intraperitoneal injection and those in lignans group were given lignans(200 mg·kg-1·d-1) via gavage; the mice in the control group and model group were given normal saline; the experiment lasted for 30 days.Twenty-four hours after the last treatment,all the mice were sacrificed and testis and epididymis of the mice were sampled.Superoxide dismutase(SOD),lactate dehydrogenase(LDH),Mg2+ ATP enzyme and Ca2+ ATP enzyme in testicular tissue were detected and sperm viability was compared between the groups and the morphology of testicular tissue were observed with hematoxylin-eosin staining.ResultsCompared with the control group,sperm motility was reduced; SOD,Mg2+ ATPase and Ca2+ ATPase contents were decreased; and LDH level was increased for the mice of the model group(P<0.05,P<0.01).Compared with the model group,sperm motility was increased,the level of SOD was increased,and that of LDH was decreased in the mice of lignans group(P<0.05,P<0.01).ConclusionLignans could protect spermatogenesis from lead acetate-induced damages in mice and the mechanism of the effect may related to anti-oxidativation of lignans.
Comparative study on utilization of two cell lines in liver cell oxidative damage model
JIN Ming, WANG Yu-jiao, JIN Mei-hua.et al
2015, 31(3): 324-326. doi: 10.11847/zgggws2015-31-03-21
Abstract(1255) HTML (1174) PDF 937KB(158)
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ObjectiveTo compare the utilization of HepG2 and Chang liver cell lines in hydrogen peroxide(H2O2)-induced hepatotoxicity cell models in vitro.MethodsMethylthiazolyldiphenyl-tetrazolium bromide(MTT)assay was used to evaluate the inhibitory effect of H2O2 on cell proliferation.Lactate dehydrogenase(LDH),alanine amino-transferase(ALT),and aspartate aminotransferase(AST)in culture solution and malondialdelyde(MDA),superoxide dismutase(SOD),and reduced glutathione(GSH)in the cells were detected with spectrophotometric method.ResultsH2O2 at the concentrations of 75-600 μmol/L and with the treatment time of 0.5 to 4 hours inhibited the proliferation of the two cell lines and resulted in the leakage of cellular LDH,ALT and AST into culture solution in a concentration- and time-dependent manner;furthermore,H2O2 increased MDA formation and reduced GSH level in the cells of the two cell lines.The results suggested that H2O2 could induce cellular oxidative injury in both HepG2 and Chang liver cell line.With the optimal H2O2 concentration of 300 μmol/L and treatment time of 4 hours,the proliferation inhibitory ratio,the activities of ALT,AST and LDH in the culture solution,and the concentration of MDA in the cells were 62%,18.2±0.2 U/L,34.2±4.6 U/L and 544.2±26.8 U/L,and 8.6±1.1 nmol/mg prot for the model utilizing HepG2 cell line,and those indicators were 76%,19.1±0.1 U/L,30.3±2.5 U/L and 536.8±22.3U/L,and 7.8±0.9 nmol/mg prot for the model utilizing Chang liver cell line.ConclusionThe two cell lines could be utilized in the construction of H2O2-induced cellular oxidative damage.
Cloning expression and sequence analysis of heat shock protein 20 of Musca domestica
PENG Chuan-lin, WANG Yu, WU Jian-wei.et al
2015, 31(3): 330-333. doi: 10.11847/zgggws2015-31-03-23
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ObjectiveTo analyze the structural characteristics of heat shock protein 20(HSP20) from Musca domestica and to clone and express the novel gene HSP20.MethodsUsing bioinformatics software package and tools of bioinformatics at web sites of National Center for Biotechnology Information(NCBI),ExPaSy,and combining other bioinformatics software packages,we identified full-length genes encoding HSP20 proteins from the Musca domestica genome library of GenBank,then predicted and analyzed the characteristics of the deduced proteins.The genes encoding HSP20 was amplified with PCR,and cloned into a prokaryotic expression vector PEASY-E1.The recombinant plasmids were transformed into E.coli OrigmiB/DE3 and then the expression of the proteins was induced by isopropyl-beta-D-thiogalactopyranoside(IPTG).The recombinant proteins were purified by Ni-IDA affinity chromatography and tested with sodium dodecyl sulfate polyacrylamide gel electophoresis(SDS-PAGE).ResultsThe full length sequence was 865 bp,containing an open reading frame(ORF)of 567 bp,encoding 188 amino acids with a predicted molecular weight of 21443.2 Da and an isoelectric point of 5.96.PCR,double enzyme digestion and DNA sequencing confirmed that the recombinant expression plasmid was successfully constructed.With SDS-PAGE,the recombinant plasmid PEASY-E1-SP20 was expressed and purified in OrigmiB/DE3,and the molecular weight of 21443.2 Da was noted in HSP20 protein bands.The highest amount of protein expression was generated 12 hours after the induction.The size of protein obtained by SDS-PAGE corresponded with target protein.ConclusionA novel gene coding HSP20 of Musca domestica was cloned,expressed,purified successfully.The purified protein of HSP20 will be of importance for further research in biological function of the gene.
Inhibitory effect of luteolin on aminopeptidase N in glioma cells and its mechanism
GAO Chun-yan, NIE Zhen-gui, WANG Jun-ya.et al
2015, 31(3): 334-337. doi: 10.11847/zgggws2015-31-03-24
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ObjectiveTo study inhibitory effect of luteolin on aminopeptidase N(APN)in glioma cells and its mechanism.MethodsEnzyme inhibitory kinetics was adopted to analyze the inhibitory effect and the kinetic constant of luteolin on APN.Growth inhibition test was conducted to assess the apoptosis of U87 glioma cells.ResultsAs a competitive inhibitor of APN,luteolinn could induced apootosis in U87 glioma cells,with a half inhibition concentration(IC50)of 70.85 μmol/L and an inhibition constant(Ki)of 24.83 μmol/L.Inactivation time process analysis showed that mignonette element could react with APN and reduce the activity of APN rapidly.ConclusionLuteolin is a competitive APN inhibitor and could reduce the activity of APN,playing an important role in the apoptosis of U87 glioma cells.
Inoculation rate and knowledge, attitude and practice about influenza vaccine among grassroots medical staff in Qingdao, China
HUANG Ling-yu, HAO Xiao-ning, BO Tao.et al
2015, 31(3): 337-340. doi: 10.11847/zgggws2015-31-03-25
Abstract(1227) HTML (278) PDF 926KB(103)
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ObjectiveTo investigate the awareness of influenza,influenza vaccine,and inoculation rate of influenza vaccine and its related factors among community medical staff and to provide a basis for promoting influenza vaccination.MethodsWe conducted a questionnaire survey in 233 health care workers(HCWs)from 7 first level hospitals in Qing-dao city selected with cluster random sampling and obtained general information, knowledge about influenza and influenza vaccine, influenza vaccination during the winter season of 2012-2013, and the willingness to accept influenza vaccination of in the winter season of 2013-2014 among the HCWs.ResultsThe coverage rate of influenza vaccination in the HCWs was 12.88%.Only 13(5.58%) of the HCWs were very likely to accept influenza vaccination.The status of knowledge, attitude and practice(KAP)of influenza and influenza vaccination was not good.The awareness rate of influenza virus was 19.31% and that of the best time for influenza vaccination was 75.97%.The status of KAP of influenza and influenza vaccination in the vaccinated group were generally better than those in the unvaccinated group.ConclusionThe coverage rate of influenza vaccination was low;the willingness to accept influenza vaccination was not strong and cognition on influenza and influenza vaccination was not good enough among grassroots HCWs.The results suggest comprehensive intervention on influenza vaccination needs to be promoted among the HCWs.
Trends and characteristics of gastric cancer mortality in China,2006-2012
GUO Kang, ZHOU Mai-geng, PAN Xiao-ping
2015, 31(3): 341-343. doi: 10.11847/zgggws2015-31-03-26
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ObjectiveTo analyze distribution features and variation trends of gastric cancer mortality in China,2006-2012.MethodsThe data on gastric cancer mortality from 2006 to 2012 were collected from National Disease Surveillance System and analyzed to estimate gastric cancer mortality by gender,urban and rural areas,and geographic locations.The changes in standardized mortality of gastric cancer were described and the annual change percentages in age-specific standardized mortality were estimated using log linear regression method.ResultsIn China,the standardized gastric cancer mortality showed a downward trend in 2006-2012.In 2006,the gastric cancer standardized mortality was 31.84/100 000 among males and 13.32/100 000 among females; whereas in 2012,the standardized gastric cancer mortality of males and females reduced to 26.51/100 000 and 10.95/100 000,respectively.The annual decrease rate of gastric cancer mortality was 3.73% for male and 4.40% for female,and the annual decrease rate was the highest among females in rural areas(4.69%) and the rate was 3.82% among rural males.The decrease rate was the lowest among urban females(3.34%).ConclusionThe gastric cancer mortality presented a downward trend,especially in the rural areas,from 2006 to 2012 in China.
Prevalence and risk factors of H-type hypertension among community residents in Jinzhou city
LI Dan, LI Xiu-hua, LIU Kun.et al
2015, 31(3): 343-345. doi: 10.11847/zgggws2015-31-03-27
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ObjectiveTo explore the prevalence and common risk factors of hypertension comorbid with hyperhomocysteinemia(H-type hypertension)among community residents in Jinzhou city.MethodsWe selected residents aged more 18 years from two communities in Jinzhou city with random cluster sampling and conducted a questionnaire survey on risk factors of H-type hypertension.ResultsTotally 2 457 residents participated the survey and the response rate was 98.45%.Of all the participants,681 were hypertensives and the hypertension prevalence rate was 28.15%,and 475 were H-type hypertension patients and the prevalence rate of H-type hypertension was 19.63%.The results of univariate analyses demonstrated that the factors related to H-type hypertension included at the age of 50-60 years,male gender,obesity,abdominal obesity,tobacco smoking,meat-eating dietary habit,lack of exercise,comorbid with coronary heart disease and metabolic syndrome,and familial history of H-type hypertension and stroke(all P<0.05).Multivariate logistic regression analyses showed that the significant risk factors of H-type hypertension were at the age of 50-60 years(odds ratio[OR]=2.870, 95% confidence interval[95%CI]=2.049-3.270),abdominal obesity(OR=2.511,95%CI=1.838-2.942),tobacco smoking(OR=3.175,95%CI=2.308-3.365),comorbid with coronary heart disease(OR=3.225, 95%CI=2.165-3.513),and with familial history of stroke (OR=3.225, 95%CI=2.165-3.513).ConclusionThe prevalence of H-type hypertension probably is closely related with aging,abdominal obesity,tobacco smoking,coronary heart disease,and familial history of stroke among community residents in Jinzhou city.
Current situation and determinants of organizational commitment among health workers in township hospitals
ZHAO Shi-chao, MENG Qing-yue, FU Ya-qun.et al
2015, 31(3): 346-348. doi: 10.11847/zgggws2015-31-03-28
Abstract(1141) HTML (241) PDF 908KB(122)
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ObjectiveTo analyze current situation and work-related determinants of organizational commitment among health workers in township hospitals,and to provide evidences for improving the stability of rural health workers.MethodsTotally 803 health workers from 45 township hospitals were selected to fill out a questionnaire about organizational commitment and its motivators.ResultsThe rank of scores for various dimensions of organizational commitment from high to low was normative commitment(14.92±2.71),affective commitment(14.08±2.92),ideal commitment(12.87±3.03),economic commitment(12.13±2.96),and opportunity commitment(11.17±2.93).Most of the health workers were satisfied with the relationship with patients,coworkers,and the leaders,but not satisfied with the income,welfare,training opportunity,and career development.The results of multivariate linear regression revealed significant incentive factors for various dimensions of organizational commitment,with the regression models of economic promise=0.365 x income,affective commitment=0.247 x income+0.508 x living environment+0.501 doctor-patient relationship+0.284 x workload+0.588 x leadership ability and style,ideal promise=0.408 x welfare+0.417 x living environment+0.358 x training opportunity+0.415 x management system+0.461 x scope of demonstrating personal ability,chance promise=0.448 x income+0.373 x training opportunity-0.382 x colleague relationship,and normative commitment=0.392 x working conditions+0.309 x doctor-patient relationship+0.426 x leadership ability and style,respectively(all P<0.05).ConclusionMost health workers have a positive attitude towards their organization and both financial and nonfinancial incentive should be improved to enhance organizational commitment in rural health workers.
Behavioral and linguistic characteristics of microblog users with various suicide ideation level:an explanatory study
GUAN Li, HAO Bi-bo, CHENG Qi-jin.et al
2015, 31(3): 349-352. doi: 10.11847/zgggws2015-31-03-29
Abstract(1255) HTML (389) PDF 923KB(111)
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ObjectiveTo compare the differences in linguistic and behavioral features of microblog users with various suicide ideation level and to identify the users with high suicide ideation for possible real-time assessment of suicide probability through social network platform.MethodsMicroblog users were recruited to participate an online survey and 982 respondents completed the survey.The respondents were divided into either high or low suicide ideation group according to their scores measured with the Suicide Probability Scale.The differences between the two groups in 10 behavioral and 88 linguistic features in microblog activities were analyzed.ResultsThe average Suicide Probability Scale score was 69.7±11.9 for all respondents;compared to the male respondents,the female had a higher subscale score of hostility(13.2±2.6 vs.12.7±2.4,P<0.05)and scaled a lower subscale score of desperation(24.4±4.7 vs.25.0±4.7,P<0.05).Suicide ideation level was negatively correlated with social activeness and the frequency of future word use(r=-0.082,-0.073,all P<0.05)and positively correlated with nocturnal activeness and frequent use of third person singular and negative word(r=0.081,0.077,0.066,all P<0.05).The respondents of high suicide ideation were less social interactive,expressed themselves less in first person plural,used "future words" less frequently,were more active at night,and used "death words" more frequently than those of low suicide ideation group(P<0.05).ConclusionMicroblog users with higher suicide ideation demonstrate significant differences from those with lower suicide ideation in terms of linguistic and microblog behavioral features.The study supports further exploration on online identification of suicidal individuals.
Association of perception of personal discrimination with coping style among left-behind children
MIAO Li-jun, XU Xiao-fang, SHENG Shi-ming
2015, 31(3): 353-355. doi: 10.11847/zgggws2015-31-03-30
Abstract(1212) HTML (265) PDF 918KB(116)
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ObjectiveTo study the association of perception of personal discrimination with coping style among left-behind children and to provide evidences for mental health promotion among the children.MethodsUsing a self-developed perception of personal discrimination questionnaire(PPDQ)and coping style questionnaire(CSQ),we surveyed 159 left-behind children and 146 non left-behind children.ResultsCompared to the non left-behind children,the left-behind children had significantly higher domain scores of PPDQ for overall(1.37±0.41),idea(1.21±0.43),contact behavior(1.31±0.59),adverse influence contribution(1.38±0.59),mistake-making tendency(1.31±0.58),and the way to be treated(1.64±0.67)perception of personal discrimination and higher domain scores of CSQ for self-blame(0.41±0.28)and rationalization(0.45±0.24)(all P<0.05).The overall perception of personal discrimination showed positive function in predicting self-blame(β=0.269,P=0.000)and rationalization(β=0.228,P=0.002)and negative function in predicting solving problems(β=-0.178,P=0.016);the perception of personal discrimination for the way to be treated had positive function in predicting self-blame(β=0.252,P=0.001)and rationalization(β=0.238,P=0.001);the perception of personal discrimination for contact behavior had negative function in predicting seeking help(β=-0.169,P=0.022);the perception of personal discrimination for adverse influence contribution had negative function in predicting solving problems(β=-0.152,P=0.040).ConclusionThe perception of personal discrimination has negative influence on coping style among left-behind children.
Influences of different anticoagulants, preparation and preservation methods on detection of circulating microparticles
YANG Yong, FENG Yan-hua, QIN Chen-hao.et al
2015, 31(3): 356-358. doi: 10.11847/zgggws2015-31-03-31
Abstract(1070) HTML (414) PDF 915KB(133)
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ObjectiveTo investigate effects of anticoagulant,sample preparation,storage temperature and time on detection of circulating microparticles(MPs).MethodsThe blood samples of 40 systemic lupus erythematosus and 41 rheumatoid arthritis inpatients from the Second Affiliated Hospital of Soochow University,and 52 healthy donors were collected.MPs of the samples treated with different anticoagulants(sodium citrate,ethylenediaminetetraacetic acid-dipotassium[EDTA-K2],heparin),preparation methods(washed platelet-poor plasma[PPP],and directed PPP),storage temperatures(-4 ℃,-20 ℃,-80 ℃),and times(1,3,7,10,and 14 days)were detected with flow cytometer and compared.ResultsThe content of MPs for the samples treated with sodium citrate plasma,EDTA-K2,heparin,and without anticoagulant were 1 527.0±620.4,981.4±247.9,877.7±176.2,and 480.8±112.2 μL, respectively,with significant differences(F=9.11, P<0.01).The MPs content of directed PPP samples from systemic lupus erythematosus patients,rheumatoid arthritis patients,and healthy donors were 1 972.4±2 850.7,3 347.8±3 431.5,and 2 157.7±1 901.1 μL,respectively,and were all higher than those of washed PPP samples(1 406.4±2 205.7,2 375.6±2 500.2,and 1 502.8±1 337.1μL)(all P<0.01).Compared with those of the fresh plasma samples,the MPs content decreased significantly for the samples stored at the temperature of-4 ℃,-20 ℃,and-80 ℃ and stored for 1,3,7,and 10 days (all P<0.05),but the content showed no significant difference when stored for 14 days at the temperature of-4 ℃,-20 ℃,and-80 ℃(all P>0.05);there were also no significant differences in MPs content among the samples with various storage duration(1-14 days)at the three different temperatures(all P>0.05).ConclusionMPs content should be detected for fresh samples treated with EDTA-K2 and without of repeated washing and the storage temperature of-4 ℃,-20 ℃,and-80 ℃ could selected for short time preservation of plasma sample.
Risk factors of hemorrhagic infraction among the elderly:a case-control study
CHEN Dong-quan, ZHANG Hong, DONG Zhi
2015, 31(3): 359-361. doi: 10.11847/zgggws2015-31-03-32
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ObjectiveTo explore the risk factors of hemorrhagic infarction(HI)among senile people and to provide evidences for the prevention of HI among the elderly.MethodsIn this study,96 HI patients confirmed by head computer tomography(CT)or magnetic resonance image(MRI)and treated in a hospital from October 2011 to October 2012 were selected as HI group and 101 acute cerebral infarction patients with hemorrhage were selected as the control group.The data on clinical manifestation at the hospital admission and disease history of the cases and controls were collected and analyzed with descriptive method and multivariate logistic regression.ResultsFor the cases,the ratio of massive cerebral infarction and cerebral embolism was 62.5% and 43.75% ;the most common hemorrhagic site was cerebral cortex(75.00%),followed by basal ganglia temporal lobe(9.38%),parietal lobe of basal ganglia(9.38%),and cerebellum(9.38%);87.5% of the patients had patchy hemorrhage bleeding and 12.5% had hematoma formation.Univariate analysis revealed that the risk factors of HI were cerebral embolism,cortical infarction,extensive infarction,diabetes mellitus,high blood sugar,history of hypertension,history of heart disease,Trial of Org 10172 in Acute Stroke Treatment(TOAST)classification,and Glasgow Coma Scale(GCS) score (all P<0.05).Logistic regression analysis showed that cerebral embolism,cortical infarction,extensive infarction,hyperglycemia at the admission,and hypertension were independent risk factors for the onset of HI.ConclusionThe incidence of HI is related to the type of cerebral infarction,the size of cerebral infarction,the location of cerebral infarction,blood pressure,and blood sugar.
Health effects of long-term exposure to low-dose occupational radiation among radiology workers
ZHANG Qi-bo, JIAO Yong-fa, LIU Jing-wei.et al
2015, 31(3): 362-364. doi: 10.11847/zgggws2015-31-03-33
Abstract(1134) HTML (407) PDF 915KB(141)
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ObjectiveTo study health effects of long-term exposure to low-dose ionizing radiation among radiology workers.MethodsCross-sectional survey and physical examination,including general health status,hemogram,lymphocyte micronucleus,chromosomal aberration,lens turbidity,and thyroid function,were conducted among 624 radiology workers in 6 class-three hospitals in Linyi city in 2013.ResultsThe average annual dosage of occupational radiation was less than 5 mSv for 97.28% of the workers surveyed;the rate of lymphocyte micronucleus and chromosomal aberration were 57.85% and 53.21% among the workers.The hemogram changes were significantly influenced by the years,annual average and accumulated dose of radiation exposure(all P<0.05)and significant differences were also observed in levels of thyroid hormones,the rates of lymphocyte micronucleus,chromosomal aberration,and lens turbidity among the radiology workers with different years and accumulated dose of occupational ionizing radiation(all P<0.05).ConclusionLong-term low-dose occupational radiation may cause certain radiation injures and radiation protection should be improved among radiology workers.
Effect of vitamin A supplementation on iron nutrition among healthy adults:an intervention study
YAN Xu-biao
2015, 31(3): 365-366. doi: 10.11847/zgggws2015-31-03-34
Abstract(1065) HTML (254) PDF 910KB(74)
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ObjectiveTo explore the effect of supplementary vitamin A(VA)on nutrition status and inadequate intake of iron among healthy adults.MethodsA total of 120 healthy volunteers were recruited and randomly divided into a control group and three VA intervention groups(taking a tablet with different doses VA once per 5 day for six months).Fasting venous blood samples of the participants were collected before and after the intervention for related detections.ResultsThere were no significant differences in age,body mass index,serum total cholesterol,serum triglyceride,low-density lipoprotein,dietary VA intake,and iron intake(all P>0.05)but significant differences in the variation of serum retinol concentration after the intervention(P<0.05)among the 4 groups.The concentration of hemoglobin did not change significantly after the intervention among all the participants(P>0.05).After the intervention the serum iron nutrition indexes changed significantly for the participants in the group with high dose VA intervention(P<0.05)but not for those in other three groups(P>0.05).ConclusionHigh dose VA supplementation without dietary iron intervention could improve iron nutritional status among healthy adults.
Effects of pregnancy diet nutrition guidance on nutrition condition and pregnancy outcome among pregnant women
SUN Ming
2015, 31(3): 367-368. doi: 10.11847/zgggws2015-31-03-35
Abstract(1178) HTML (251) PDF 908KB(110)
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ObjectiveTo explore effects of diet nutrition guidance on nutrition status and pregnancy outcome among pregnant women.MethodsDuring the period of October 2012 through October 2013,500 healthy pregnant women participated in this study.According to the principle of voluntary,the pregnant women were randomly divided into an observation and a control group;the women in the observation group were given routine health guide and standardized individualized-dietary nutrition guidance and those in the control group were given routine prenatal health guidance.Then we analyzed the differences in nutritional status in late pregnancy and pregnancy outcome among the women of the two groups.ResultsCompared to the women in the control group,the women in the observation group had significantly lower weight gain during pregnancy and higher intakes of protein,folic acid,calcium,iron,and zinc(all P<0.05);the incidence of pregnancy-induced hypertension,macrosomia,and intrauterine growth delay were also significantly lower among the women in the observation group than among those in the control group(all P<0.05).ConclusionStrengthening dietary nutrition guidance during pregnancy among pregnant women could improve nutritional status and reduce the occurrence of adverse pregnancy outcome among the women.
Association between RETN gene polymorphisms and type 2 diabetes mellitus
XIU Liang-chang, REN Han, YANG Jie.et al
2015, 31(3): 369-372. doi: 10.11847/zgggws2015-31-03-36
Abstract(1009) HTML (320) PDF 938KB(98)
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ObjectiveTo evaluate the association between two single nucleotide polymorphisms(SNPs)located in RETN gene and type 2 diabetes mellitus(T2DM)in Han population in Guangdong province.MethodsTwo tag SNPs(rs7408174 and rs3219175)were picked out from RETN gene.Then SNP genotyping was performed using SNPscanTM Kit in 595 cases and 725 controls.Chi-square test,logistic regression model were employed in statistical analyses.ResultsThe frequencies of TT,CT and CC of rs7408174 were 329(55.3%),230(38.7%),and 36(6.1%)in the cases and 366(50.8%),308(42.8%),and 46(6.4%)in the controls,respectively.The frequencies of GG,GA and AA of rs3219175 were 377(63.4%),200(33.6%),and 18(3.0%)in the cases and 442(61.0%),253(34.9%),and 30(4.1%) in the controls,respectively.No association between SNP of rs7408174 and T2DM was found in allelic association analysis and genotypic association analysis after adjustment for sex,body mass index and triglyceride.The other SNP of rs3219175 was not associated with T2DM either.ConclusionNeither rs7408174 nor rs3219175 of RETN gene was associated with T2DM.
Epidemic of severe fever with thrombocytopenia syndrome in Dalian city,20112013
LIANG Yu-hong, QI Shang, ZHOU Ling.et al
2015, 31(3): 373-374. doi: 10.11847/zgggws2015-31-03-37
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ObjectiveTo analyze epidemiological characteristics of severe fever with thrombocytopenia syndrome(SFTS)and to provide a basis for the development of effective prevention and treatment strategies.MethodsWith descriptive epidemiological method,data on 55 SFTS cases reported in Dalian city during the period of 2011—2013 were collected and analyzed.ResultsThe cases were distributed in 7 administrative areas,with obviously different incidences across various areas.The cases occurred from May to October in a year and 67% of the cases were reported from July to August.The male to female ratio of the cases was 1.29:1.The age distribution of the cases was 34 to 89 years,with a mean age of 64.02 years.The majority of the cases were farmers,housework workers,and the unemployed.ConclusionThe SFTS incident has an obvious seasonality,which may be related to exposures to ticks,in Dalian city.
Determination of trace barium in drinking water by optimized GFAAS
ZHANG Xin, MA Jin-bo, FU Ying.et al
2015, 31(3): 375-376. doi: 10.11847/zgggws2015-31-03-38
Abstract(1002) HTML (332)
Abstract:
ObjectiveTo optimize the instrumentation condition and matrix modifiers of graphite furnace atomic absorption spectrometry(GFAAS)to determine trace barium in drinking water.MethodsThe drinking water samples were mixed and filtered,with calcium nitrate(Ca[NO3]2)as the matrix modifier;then the content of barium in the samples was analyzed with optimized GFAAS.ResultsThe lowest detection limit of the method was 3.1×10-12 g;the recovery of standard addition was 94.0% -98.0% ;the relative standard deviation(RSD)was less than 7.6% ;and the linear range was 0-50 μg/L,with a correlation coefficient of 0.9996.ConclusionThis method is sensitive,accurate for determination of trace barium in drinking water.
Pretreatment method for determination of aluminum in meat and meat products with ICP-OES
HUA Zheng-gang, YI Ping, FENG Jing.et al
2015, 31(3): 377-378. doi: 10.11847/zgggws2015-31-03-39
Abstract(1063) HTML (324) PDF 1578KB(99)
Abstract:
ObjectiveTo establish a pretreatment method for the determination of aluminum(Al)in meat and meat products with inductively coupled plasma optical emission spectrometry(ICP-OES).MethodsSixty meat samples were pretreated with wet digestion,microwave digestion,and dry ash method and the experimental conditions for the three methods were optimized.ResultsThe experimental results show that dry ash method is best for the determination of Al in meat and meat products and an additional digestion by adding a moderate a mount of nitric acid when necessary.The detection limit is 0.6 mg/kg,the range of recovery rate is 86.0% -105%,and the relative standard deviation(RSD)is 8.8% for the Al detection under dry ash pretreatment.ConclusionThe method for determination of Al in meat samples is better than the methods currently used.
Health effects of drought:a review of recent studies
WANG Ning, LI Jie, LI Xue-wen.et al
2015, 31(3): 379-382. doi: 10.11847/zgggws2015-31-03-40
Abstract(1363) HTML (415) PDF 936KB(151)
Abstract:
Drought causes marked negative impact on human health.In this article,the influences of drought on human health in the aspects of nutritional status,digestive diseases,respiratory diseases,vector-borne diseases,mental health,death,cancer,and even gynecological diseases were summarized.Some currently accepted and controversial conclusions on the health effects of drought were discussed and the possible and uncertain influence factors were demonstrated.And then,the problems need to be solved in future research were pointed out.This article provides basic information for the study on the impact of drought on human health.
Identification and drug sensitive test of bacteria isolated from clinical specimens:a hospital-based analysis
LI Yu-mei, AN Hong-zhi
2015, 31(3): 383-384. doi: 10.11847/zgggws2015-31-03-41
Abstract(1339) HTML (418) PDF 902KB(101)
Abstract:
ObjectiveTo investigate the results of bacterial culture and drug sensitivity test for clinical specimens collected in the Central Hospital of Chaoyang City during the first half of 2012.MethodsIdentification and drug sensitive test were conducted for bacterial isolations of various clinical specimens from out- and in-patients of the hospital.ResultsTotally 1 024 bacterial strains were isolated from 2 764 clinical specimens,of which,705(68.8%)were gram-negative bacilli and 238(23.2%)were gram-positive cocci.The main gram-negative bacilli isolated were Pseudomonas aeruginosa(20.4% of the all isolated strains),Bauman Acinetobacter(17.5%),Escherichia coli(14.7%),and Klebsiella pneumoniae genus(13.9%) and the major gram-positive cocci were coagulase negative Staphylococcus(12.7%) and Staphylococcus aureus(9.96%),respectively.The gram-negative bacilli isolated were sensitive to amikacin,imipenem,meropenem,cephalosporin cefepime,and piperacillin/tazobactam and the gram-positive cocci were sensitive to minocycline,vancomycin,and rifampin.ConclusionMonitoring on clinically isolated bacteria and their drug resistance is important to rational use of antimicrobial agents and effective control of infection in clinical practices.
论文
Resvertrol improves memory in AD rats by down-regulating expression of -APP in hippocampus
LI Na, CHENG Xue-jiao, WANG Qian.et al
2015, 31(3): 327-329. doi: 10.11847/zgggws2015-31-03-22
Abstract(1228) HTML (330) PDF 940KB(119)
Abstract:
ObjectiveTo evaluate whether long-term resvertrol(Res)treatment could result in the attenuation of spatial memory impairment in Alzheimer's disease(AD)rats and its mechanism.MethodsSixty female Wistar rats were divided into an AD model group(treated with ovariectomy[OVX]plus D-galactose[Gal] at 100 mg/kg),low,moderate,and high Res groups(OVX,Gal and Res at 20,40,80 mg/kg),estradiol valerate group(OVX,Gal and estradiol valerate at 0.8 mg/kg),and sham control group.At the end of 12 weeks,spatial memory was checked with Morris water maze test(MWM) and the expression of β amyloid precursor protein(β-APP)in hippocampus of the rats was test with Western blot.ResultsThe escape latency to find the submerged platform significantly declined in the rats of all groups.Moreover, the latency in the rats of 80 mg/kg Res(16.49±4.18 s)and estradiol valerate treatment group(17.05±2.71 s)decreased significantly,while the times of crossing over the platform site for the rats in 80 mg/kg Res(3.90±0.97)and estradiol valerate treatment group (3.44±0.63)increased markedly compared with that of the model group(1.22±0.45)(P<0.05).The time of stay in the target quadrant for the rats in all of the Res treatment groups was longer than that of the model group(P<0.05).The expression of β-APP in hippocampus in the rats of 20,40 and 80 mg/kg Res treatment groups were 0.57±0.054,2.63±0.50,and 0.28±0.043,respectively,with significant decrease compared with that of the model group(0.27±0.057)(P<0.05).ConclusionThe results suggest that Res is helpful in protecting AD rats from developing memory decline by decreasing the expression of β-APP in hippocampus.