Objective To analyze the characteristics of Asian Zika virus (ZIKV) genome sequence and to establish a real-time fluorogenic quantitative PCR method for ZIKV detection.
Methods The whole genome sequence of Asian ZIKV was analyzed and the conserved region of Asian ZIKV was used to design primers and TaqMan probes. A standard curve was established and its specificity and sensitivity were assessed.
Results Detection of designed PCR primers and probes were of good specificity, with a lower detection limit of 3.415 × 100 copies/μL. There were no cross reactions between African ZIKV, hepatitis C virus and type 1 – 4 dengue virus.
Conclusion A fluorescence quantitative PCR technique for the detection of Asian Zika virus was established, which could be used in etiologic typing, early diagnosis and prevention of Asian Zika virus.
DownLoad: