Determination of bongkrekic acid in urine with high performance liquid chromatography

  Objective  To establish a method for the determination of bongkrekic acid in urine samples.

  Methods  The urine sample was centrifuged with 12 000 r/min at low temperature for 10 minutes; then the supernatant was directly injected into high performence liqiud chromatography for determination. After separated on a C18 column using methanol and acetic acid water (pH 2.5) as a mobile phase, bongkrekic acid was detected with diode array detector and quantified with blank matrix standard curve.

  Results  The linear range for bongkrekic acid detection was 0.5 – 20.0 μg/mL; the correlation coefficients were all above 0.998. The detection limit of the method was 0.3 g/mL and the quantitive limit of the method was 1.0 μg/mL. The recovery ranged from 87.5% to 113.4%, with a relative standard deviation (RSD) of less than 8.4%.

  Conclusion  The established method is simple, rapid, accurate and suitable for the determination of bongkrekic acid in urine sample.