Objective To examine the situation of Japanese encephalitis virus (JEV) infection in domestic swine in some areas of Southern China for the prevention and control of JE.
Methods A total of 465 blood samples were sterilely collected from apparently healthy swines without JE vaccine inoculation at slaughter houses in Southern China (Guangdong, Hainan and Jiangxi provinces) during January to August 2013 and detected for nucleic acid, antigen (Ag) and immunoglobulin G (IgG) antibody of JEV using TaqMan real-time reverse transcription (RT)-PCR test and enzyme-linked immunosorbent assay (ELISA) kits.
Results No JEV-RNA positive samples was observed for the 437 samples detected with TaqMan real-time RT-PCR. But for 465 samples detected using ELISA, 43 and 445 were JEV Ag and IgG antibody positive, with the positive rates of 9.2 % and 95.7 %, respectively.
Conclusion The results indicated that JEV infection is prevalent in swines in Guangdong, Hainan and Jiangxi province and attention should be paid to the situation for the prevention and control JEV infection.
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