Distribution and antibiotic resistance of Gram-negative non-fermentative bacteria isolated in hospital environment

  Objective  To examine the distribution and antibiotic resistance of Gram-negative non-fermentative bacteria isolated in medical environment.

  Methods  We collected 176 public facility and medical equipment surface swab samples and 22 swab samples of medical staff's hands after disinfection in 11 grade A tertiary hospitals in Heilongjiang province during October 2017. The identification and drug sensitivity test of the isolated bacterial strains were performed using the French MERIE Vitek microorganism identification instrument. Kirby-Bauer disk diffusion method was used in antibiotic resistance test for the isolated strains.

  Results  The total detection rate of Gram-negative non-fermentative bacteria was 7.58% for the facility and equipment surface swab samples. The main isolated bacteria were Sphingomonas paucimobilis, Acinetobacter baumannii, and Pseudomonas oryzihabitans; all the isolated strains were multiple antibiotic resistant and the highest antibiotic resistance rate (80%) was detected in the stains resistant to nitrofurans and folic acid metabolic pathway inhibitors. No Gram-negative non-fermentative bacteria positive samples was detected for the swabs of medical staff's hands.

  Conclusion  Antibiotic resistant Gram-negative non-fermentative bacteria are prevalent in medical service environment and the situation should be concerned in antibiotic selection in clinical practice.