Effect of bergenin on D-galactosamine-induced acute liver injury in rats

XU Jie; GAO Ya; JI Yan-ping

doi:10.11847/zgggws1131528

Volume 38 Issue 1

Jan. 2022

Turn off MathJax

Article Contents

Article Navigation > Chinese Journal of Public Health > 2022 > 38(1): 70-74

XU Jie, GAO Ya, JI Yan-ping, . Effect of bergenin on D-galactosamine-induced acute liver injury in rats[J]. Chinese Journal of Public Health, 2022, 38(1): 70-74. doi: 10.11847/zgggws1131528

Citation:

XU Jie, GAO Ya, JI Yan-ping, . Effect of bergenin on D-galactosamine-induced acute liver injury in rats[J]. Chinese Journal of Public Health, 2022, 38(1): 70-74. doi: 10.11847/zgggws1131528

XU Jie, GAO Ya, JI Yan-ping, . Effect of bergenin on D-galactosamine-induced acute liver injury in rats[J]. Chinese Journal of Public Health, 2022, 38(1): 70-74. doi: 10.11847/zgggws1131528

Citation:

XU Jie, GAO Ya, JI Yan-ping, . Effect of bergenin on D-galactosamine-induced acute liver injury in rats[J]. Chinese Journal of Public Health, 2022, 38(1): 70-74. doi: 10.11847/zgggws1131528

PDF( 689 KB)

Effect of bergenin on D-galactosamine-induced acute liver injury in rats

doi: 10.11847/zgggws1131528

College of Pharmacy, Guilin Medical University, Guilin, Guangxi Zhuang Autonomous Region 541004, China

Received Date: 2020-07-07
Available Online: 2021-08-12
Publish Date: 2022-01-20

Abstract

Abstract

Objective To study protective effect and mechanism of bergenin on D-galactosamine (D-GalN)-induced acute liver injury in rats. Methods Totally 60 Sprague-Dawley (SD) rats were randomly assigned into 6 groups (10 in each group): a control and a model group with saline, a silymarin group (120 mg/kg), and low, moderate and high bergenin groups (20, 40, 80 mg/kg). All the treatments were carried out by intragastric administration (10 mL/kg) once a day consecutively for 7 days. Two hours after the last administration, D-GalN (700 mg/kg) was intraperitoneally administered for all the rats to establish acute liver injury model, excepted for the control rats. Sixteen hours after the D-GalN injections, all rats′ blood and liver tissue specimens were collected. Biochemical methods were used to detect serum content or activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total antioxidant capacity (T-AOC), total bilirubin (TBIL), total superoxide dismutase (T-SOD), malondialdehyde (MDA), and glutathione peroxidase (GSH-Px); enzyme-linked immunosorbent assay (ELISA) was adopted to measure contents of interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in liver tissues; and Western blot analysis was performed to determine phosphorylated c-Jun N-terminal kinase (p-JNK), JNK, c-Jun, phospharylated adenosine monophosphate-activated protein kinase (p-AMPK), and AMPK protein expressions in liver tissues, respectively. Pathological changes of liver tissues were observed with hematoxillin-eosin (HE) staining. Results Compared with those in the control rats, serum activity of AST, ALT, TBIL and MDA were significantly increased in the model rats (all P < 0.01). In contrast to those in the model rats, serum AST, ALT, TBIL, and MDA were significantly reduced in the rats of the three bergenin groups (all P < 0.05) and the activity of serum T-AOC, T-SOD, and GSH-Px were significantly increased in the rats of moderate and high bergenin groups (all P < 0.01); in addition, significantly decreased TNF-α, IL-1β, IL-6 and increased IFN-γ (all P < 0.01), down-regulated p-JNK and c-Jun expressions (both P < 0.05) but up-regulated p-AMPK expression (P < 0.01) were detected in liver tissues of the rats of moderate and high bergenin groups. Conclusion Bergenin possesses a protective effect on D-GalN-induced acute liver injury in rats and the mechanism of the effect may be related to the inhibition of oxidative stress and the regulation of AMPK/JNK signaling pathway.

FullText(HTML)

References(24)

References

[1]	张红阳, 李雪溦, 姚雪莲, 等. 肝损伤的分子机制及其中药药理研究进展[J]. 中药新药与临床药理, 2016, 27(3): 448 – 455.
[2]	李萍萍, 杨生超, 曾云恒. 岩白菜素药源植物资源研究进展[J]. 中草药, 2009, 40(9): 1500 – 1505. doi: 10.3321/j.issn:0253-2670.2009.09.051
[3]	夏晓旦, 普天磊, 黄婷, 等. 岩白菜的化学成分、含量考察与药理作用研究概况[J]. 中国药房, 2017, 28(16): 2270 – 2273. doi: 10.6039/j.issn.1001-0408.2017.16.30
[4]	吴雅锋, 龙翠珍, 舒远辉, 等. 大麻素受体 – 2激动剂AM1241对急性肝损伤小鼠肝组织炎性体NLRP3影响[J]. 中国公共卫生, 2019, 35(5): 571 – 573. doi: 10.11847/zgggws1122242
[5]	Shu Y, He D, Li W, et al. Hepatoprotective effect of Citrus aurantium L. against APAP-induced liver injury by regulating liver lipid metabolism and apoptosis[J]. International Journal of Biological Sciences, 2020, 16(5): 752 – 765. doi: 10.7150/ijbs.40612
[6]	Peng XW, Yang YQ, Tang L, et al. Therapeutic benefits of apocynin in mice with lipopolysaccharide/D-galactosamine-induced acute liver injury via suppression of the late stage pro-apoptotic AMPK/JNK pathway[J]. Biomedicine and Pharmacotherapy, 2020, 125: 110020. doi: 10.1016/j.biopha.2020.110020
[7]	Yoshida K, Ushida Y, Ishijima T, et al. Broccoli sprout extract induces detoxification-related gene expression and attenuates acute liver injury[J]. World Journal of Gastroenterology, 2015, 21(35): 10091 – 10103. doi: 10.3748/wjg.v21.i35.10091
[8]	张立海, 慈慧. 水飞蓟素保肝的药理作用及临床应用[J]. 首都医药, 2012, 19(8): 47 – 48.
[9]	李家妮, 李梓萌, 高雅, 等. 老鹳草素对D – 氨基半乳糖诱导的肝损伤小鼠的保护作用及机制[J]. 中国实验方剂学杂志, 2019, 25(15): 116 – 121.
[10]	Zhu YH, Chen XB, Rao XL, et al. Saikosaponin a ameliorates lipopolysaccharide and D-galactosamine-induced liver injury via activating LXRα[J]. International Immunopharmacology, 2019, 72: 131 – 137. doi: 10.1016/j.intimp.2019.03.049
[11]	袁成福, 李志红, 彭帆, 等. 火棘多糖对CCl₄所致大鼠肝损伤保护作用[J]. 中国公共卫生, 2016, 32(11): 1485 – 1487. doi: 10.11847/zgggws2016-32-11-09
[12]	Lim HK, Kim HS, Choi HS, et al. Effects of acetylbergenin against D-galactosamine-induced hepatotoxicity in rats[J]. Pharmacological Research, 2000, 42(5): 471 – 474. doi: 10.1006/phrs.2000.0730
[13]	Chen XL, Wu W, Gong BM, et al. Metformin attenuates cadmium-induced neuronal apoptosis in vitro via blocking ROS-dependent PP5/AMPK-JNK signaling pathway[J]. Neuropharmacology, 2020, 175: 108065. doi: 10.1016/j.neuropharm.2020.108065
[14]	Yuan DT, Huang S, Berger E, et al. Kupffer cell-derived Tnf triggers cholangiocellular tumorigenesis through JNK due to chronic mitochondrial dysfunction and ROS[J]. Cancer Cell, 2017, 31(6): 771 – 789. doi: 10.1016/j.ccell.2017.05.006
[15]	Han RI, Zhang F, Wan C, et al. Effect of perfluorooctane sulphonate-induced Kupffer cell activation on hepatocyte proliferation through the NF-κB/TNF-α/IL-6-dependent pathway[J]. Chemosphere, 2018, 200: 283 – 294. doi: 10.1016/j.chemosphere.2018.02.137
[16]	Xiang S, Chen K, Xu L, et al. Bergenin exerts hepatoprotective effects by inhibiting the release of inflammatory factors, apoptosis and autophagy via the PPAR-γ pathway[J]. Drug Design, Development and Therapy, 2020, 14: 129 – 143. doi: 10.2147/DDDT.S229063
[17]	Chen MS, Cao LJ, Luo YJ, et al. Paeoniflorin protects against concanavalin A-induced hepatitis in mice[J]. International Immunopharmacology, 2015, 24(1): 42 – 49. doi: 10.1016/j.intimp.2014.11.006
[18]	穆凌云, 李金霞, 张秋云, 等. 截断逆挽方对慢加急性肝衰竭模型大鼠血清TNF-α、肝组织p-JNK及c-Jun的影响[J]. 首都医科大学学报, 2017, 38(2): 282 – 288. doi: 10.3969/j.issn.1006-7795.2017.02.023
[19]	张万国, 胡晋生, 蔡溱. 桑黄诱生γ-干扰素与抗肝纤维化[J]. 中医药学报, 2002, 30(6): 22 – 24. doi: 10.3969/j.issn.1002-2392.2002.06.012
[20]	Huang LM, Hu QQ, Huang XX, et al. Preconditioning rats with three lipid emulsions prior to acute lung injury affects cytokine production and cell apoptosis in the lung and liver[J]. Lipids in Health and Disease, 2020, 19(1): 19. doi: 10.1186/s12944-019-1137-x
[21]	Wang Z, Ka SO, Han YT, et al. Dihydropyranoaurone compound damaurone D inhibits LPS-induced inflammation and liver injury by inhibiting NF-κB and MAPK signaling independent of AMPK[J]. Archives of Pharmacal Research, 2018, 41(3): 314 – 323. doi: 10.1007/s12272-017-1001-3
[22]	Li LL, Chen JT, Lin L, et al. Quzhou Fructus Aurantii extract suppresses inflammation via regulation of MAPK, NF-κB, and AMPK signaling pathway[J]. Scientific Reports, 2020, 10(1): 1593. doi: 10.1038/s41598-020-58566-7
[23]	Cai L, Hu K, Lin L, et al. AMPK dependent protective effects of metformin on tumor necrosis factor-induced apoptotic liver injury[J]. Biochemical and Biophysical Research Communications, 2015, 465(3): 381 – 386. doi: 10.1016/j.bbrc.2015.08.009
[24]	Hao R, Su GY, Sun XL, et al. Adiponectin attenuates lipopolysaccharide-induced cell injury of H9c2 cells by regulating AMPK pathway[J]. Acta Biochimica et Biophysica Sinica, 2019, 51(2): 168 – 177. doi: 10.1093/abbs/gmy162