Effect of Deltamethrin on EROD activity and protein expression in rat brain

The EROD activity in rat brain was determined by flurophotometry under the treatment of DM in vivo and vitro.The results showed:in vivo,DM could markedly inhibit EROD activity in rat brain microsome after rats had been received DM(12.5mg·kg^-1·d^-1,ip)for 5 days.The inhibitory rate in whole brain,cerebral cortex were 32.0% and 37.7% respectively.However,in vitro, under the concentration of 2×10^-8～2×10^-4mol/L,there was no obvious change of EROD activity in rat brain.Moreover,Western blot analysis indicated that DM could significantly reduce the expression of C YP1A1/1A2 protein.So the mechanism by which DM inhibit EROD activity in rat brain was releated to the reduction of C YP1A1/1A2 protein expression by DM.