Stndy of Celular Tumor-Suppressor p53 Inhibit the Expression of Hepatitis B Virus X Gene in Vitro

Purpose Hepatitis BVirus(HBV) is a major risk factor for human hepatocellular carcinoma.The oncogenicity of HBV is dependent on the intigration of the HBVX gene into the hepatocellular DNA and transactive function to the cellular gene.The cellular turmor-suppressor gene P⁵³ can negatively regulate the transactive function of X gene.To examine the expression of the X gene in hepatoma cells and the inhibiting function of P 53 gene to the X gene.This test was conducted.Methods The methods were analysing the production of transient expression of transfected HBVDNA,HBVX gene and P 53 using human hepatocellular carcinoma HuH-7 and HeG 2 cells,as a recipient by cell culture in vitro and southern hybridization.Results The 2.6,2.2 and 0.9 kilobase transcripts were observed.We also found that wild-type P⁵³ markedly reduced the expreseion of HBVS gene and X gene as complexed they to transfect the hepatocellular carcinoma cells.Conclustins Results suggest that wild-type P⁵³ inhibit the transactive function of HBVX gene in the liver cells.