Study on Gene Type of Orientia Tsutsugamushi Isolated Strains by PCR/SSCP

ObjectiveUsing PCR/SSCP to analyse the gene type of the six Orientia stustugamushi(Ot)strains isolated from Liaoning and Jilin areas and making a comparision between the isolated strains of Ot and the international reference strains.MethodsThe six strains from Liaoning and Jilin appeared two different kinds spectrum of SSCP:The strains HCAa9202,HCAp9203 and HCM 9501 had the same gene type with the reference strain Gilliam;strains KDCt9402,KDCp9403 and KDCt9404 had the same gene type with reference strain Karp.ResultOt strains isolated from Liaoning and Jilin areas had at least two different types identified by PCR/SSCP analysis.ConclusionThe method of PCR/SSCP was proved to be simple,economic,time-saving and accurate.