Phylogenetic Analysis of Partial cDNA Sequence of Hepatitis C Virus Derived from a Patient with HCV Infectinn in China

Objective To clone and sequence partial cDNA of Hepatitis C virus(HCV)genome of Shanxi China,constructed phylogenetic tree.Methods A 878bp fragment in envelope region was amplified by reverse transcr iption nested polymerize chain reaction(RT-PCR).The fragment was cloned into PGEMT-vector,then sequenced by auto-sequencing instrument.Phylogenetic tree were constructed from the 5'NCR sequene of 252bp,C sequence of 436bp and E sequence of 878bp by HCV-XA and other 36 strains registered in Genbank respectively.Results Phylogenetic tree constructed from the 5'NCR indicates HCV-XA belonged to type 2;from C and Eindicate it belonged to subtype 2a.Conclusion The isolated strain belonged to type 2a.The variation of the subgenome affected the result of phylogenetic analyses for the same pathogen.