Construction of Rotavirus Vaccine Plant Cell Expression Vector and Expression of GUS Gene

Objective Plant cell expression system would be established to express rotavirus structural protein.Methods VP4 gene fragment of rotavirus were amplified by RT-PCR,and were cloned into plant cell expression vector RG-2.The positive clones were selected by dothybridization with Dig-labeled probe.In this study,β-glucuronidase(GUS) were transmitted into potato cells.Results VP4 gene was 960bp as long as designed,and four positive clones were detected and blue matters were found in GUS gene transmitted plant cells.Conclusion GUS activity could be detected in potato cells and a fine transgenic method was established.This gene expression system would be useful for preparing new rotavirus vaccine.