Time-Course Changes of Metallothionein Gene Expression Induced by Cadmium Metallothionein in Mice’s Kidney

ObjectiveTime-course of gene expression of metallothionein(MT1 and MT2)in kidney conex from mice were measured.MethodsFollowing an initial high CdMT dose and four subsequent low doses(50+4×25μg/kg)administered subcutaneously at an interval of 2h,with semi-quantitative R T-PCR.ResultsThe MTI gene expression level was higher at 12 h and returned its basal level at 168h after first injection.The MT2 gene expression level was significantly higher at 12 and 24h and also returned its basal level at 168h.The MT1 and MT2 gene expression level reached to the maximum at 24h.ConclusionMT1 and MT2 genes in mice's kidney cortex from mice could be induced by multiple short-interval CdMT injections.Time course of MT1 and MT2 gene expression was parallel.