Protective effects of extracts from leaves of Ginkgo biloba on TCE-induced oxidation insult in human keratinocytes

  Objective   To fur ther assess the effects of extracts from the leaves of Ginkgo biloba on Trichloroethy lene (TCE)induced normal human epidermal keratinocytes(NHEK)oxidation insult.

  Methods   Cytotoxicity was determined by netrualred uptake(NRU)and lactate dehydrogenase release(LDH)test, lipid peroxidation of cells was evaluated by malon dialdehyde(MDA)and superoxide dismutase(SOD), respectively.

  Results   Cytotoxicity asays yielded the concentration producing 50% reduction of Netrual Red Uptake(NR50)for TCE was 4 53 mmol/L.Compared with control, treatment of with various concentration of TCE caused a great decrease in cell viability(P < 0.05).A concentration and time dependent release of LDH and a concentration dependentincrease of MDA levels were observed.In contrast, SOD activity was inhibited by concentration dependent mode.Ginkgo biloba 10~200 mg/L dose dependently attenuated the cytotoxic effect of TCE.Ginkgo biloba also inhibited TCE(0.5 mmol/L)induced LDH release, elevation of lipide peroxidation and decline of antioxidant enzyme activities.

  Conclusion   Ginkgo biloba protected NHEK from TCE induced cytotoxicity.