Comparing studies of blood sample treatments for ApoE gene polymorphism analysis

  Objective   For the larger scale epidemiological study on apolipoprotein E(ApoE)gene polymorphism analysis.To compare the different blood sample treatments.

  Methods   ApoE allele poly mor phism analysis enthod was established in our lab, and the different trace DNA extraction methods of destilled waterextr action method, blood cell lysis method and Tris EDTA extraction method were compared.

  Results   The most sensitive method was distilled waterextract ion method.As to the effect of blood dryness on filter, the best result could be achieved from over 24 h drying.As to economy and convenience of the methods, this laboratorys ApoE analysis system had the same results with the import reagent kit but cheaper.

  Conclusion   The distilled waterextraction method for ApoE allele polymor phism analysis was proper for larger scale epidemiological study, which had the advantages of quick, convenient and sensitive characters.