Study on damage of germ cells induced by inhalation carbon disulfide in mice

  Objective   To study the damage effect of inhalation carbon disulfide on germ cells of mice.

  Methods   The damage of germ cells of mice exposed to carbon disulfide by 2 h static inhalation per day at different concentrations(0, 200, 400, 800 mg/m³)for 5 weeks was analyzed with single cell gel electrophoresis(SCGE)and flow cytometry(FCM).

  Results   In four groups, the rate of DNA damage were 48.50%, 71.00%, 86.50% and 23.50% respectively, there was significant difference between the experiment group and control(P < 0.01);the percentage of head of the coment were(62.9949±8.6242)%, (56.6580±10.5990)%, (36.5432±12.0002)% and(86.8926±5.0219)% respectively; there was significant difference between the experiment group and control and every experiment groups(P < 0.001);the tail length in every groups were(8.6812±4.333), (14.0392±4.168), (28.6601±8.369)and(5.9913±1.924)m respectively, there was significant difference between middle, high dosage groups and control(P < 0.001).The rate of apoptosis in all groups were 12.53%, 14.93%, 29.09% and 11.97% respectively, and only high dosage group had significant difference compared with the control.

  Conclusion   The sub chronic carbon disulfide exposed can induce DNA damage of germ cells.