Effect of fish oil and transmembrane TNFα eukaryotic expression vector on breast cancer xenograft growth in nude mice

  Objective   To explore the effectof fish oil and transmembrane TNFA eukaryotic expression vector on breastcancerx enograft growth in nude mice.

  Methods   MCF-7 cells were transplanted subcutareously to BALB/c nude mice to produce tumor.The nude mice with x enograftwere randomly divided intofour groups:control group, fish oil treated group, TNFA transfacted group and fish oil+TNFA transfacted group.The xenog rafttumor growth in mice was observed.The cell cycle was measured by using flowcy to meter.The expression of transmembrane TNFAmRNA was deter mined by RT-PCR.

  Results   Fish oil significantly inhibited the tumor growth in vivo.The cell cycle analysis showed thatfish oil blocked the tumor cells atthe G1/G0 phage so as to reduce the ratio of Sphage.These chang es were more obviously in fish oil+TNFA transfacted group than those of fish oil treated group.The expression of transmembr ane TNFA mRNA was increased in fish oil +TNFA transfacted group.

  Conclusion   Fish oil could induce expression of transmembrane TNFA in xenog rafttumor transfacted with pPPRE-tk-tmTNFA, and this could promote the anti-tumor activity of fish oil in vivo.