Detection of human herpesvirus 8 from kaposi's sarcoma by nested-PCR

  Objective   To confirm the relationship between Human herpesvirus 8(HHV8)and the pathogeny of Kaposis sarcoma(KS)in Xinjiang.

  Methods   Nested-PCR was applied to detect HHV8 DNA that extracted from KS serum(or biopsy)and non KS serum.

  Results   HHV 8 DNA was detected in 25 out of 29(86.21%)KS serum, and 41 out of 43 KSbiopsy.The difference between the two type of samples was not significant(χ²=1.90, P=0.173).And in non KS controls, 14 out of 68(20.59%)were positive.The differences were statistically significant between the KS group and non KS group (χ²=72.15, P < 0.001).

  Conclusion   These data imply that HHV 8 is the pathogeny of KS, and a more sensitive method was established to detect HHV 8 DNA from serum by nested-PCR.