Relationship between human 8-hydroxyguanine glycosylase Ser326Cys gene polymorphism and esophageal cancer

  Objective   To determine the association of the human 8 hydrox yguanine glycosylase(hOGG1)Ser326Cys polymorphism and esophageal cancer risk in Huaian.

  Methods   hOGG1 genot yping was performed by diallele specific amplification with artificially modified primer(diASA-AMP)analysis of genomic DNA isolated from 106 pairs of esophageal cancer cases and non cancer controls individually matched by residence, sex and age.The association of the genetic polymorphisms and cancer risk was evaluated by a multivariate analysis.

  Results   The frequency of Ser/Ser, Ser/Cys, and Cys/Cys genoty pesin the control subjects were 19.8%, 47.2% and 33.0% respectively, and there was no significant difference in the distribution of the hOGG1 Ser326Cys polymorphism between this studied group and other reported Chinese groups.The hOGG1 genotypes of 10 random samples were verified with DNA sequencing.No significant difference was observed in the frequency of hOGG1 geno types between controls and cases(χ²=1.439;P=0.696).No significantly increased risk for esophageal cancer was observed for those carry ing 326Cys allele with Logistic regression analysis(OR=1.385;95% CI=0.678~2.823).And also no statistically significantinter actions between hOGG1 Ser 326Cys polymorphism and smoking or other possible confounding factors were observed for esophag eal cancer risk.

  Conclusion   hOGG1 Ser326Cys polymorphism is unlikely to be as sociated with the susceptibility to esophageal cancer.The polymorphisms play no role in the risk of smoking for esophageal cancer.The diASA-AMP method in an available technique for single nucleotide polymorphism(SNP)rapid determination with its ideal specificity.