Correlation analysis on telomere regulating factor tankyrase and telomerase reverse transcriptase in gastric adenocarcinoma

  Objective   To detect the expression level of telomere regulating factor Tankyrase and human telomerase reverse transcriptase(hTERT)in gastric adenocar cinoma and further elucidateits correlation.

  Methods   Quantitative real-time PCR was used to measure expression of Tankyrase and hTERT mRNA in canerous and normal adjacent tissues from 16 patients with gastric adenocar cinoma.

  Results   The expression of Tankyrase mRNA in gastric adenocarcinomas(1.44×10^-2, 3.88×10^-5~0.4847)was significantly higher than that in normal adjacent mucosas(1.0134×10^-2, 0~41.933×10^-2)(P < 0.05).The expression of hTERT mRNA in gastric adenocarcinomas(1.235×10^-4, 0~1.330 1)was also significantly higher than that in normal adjacent mucosas(0, 0~4.38×10^-5)(P < 0.01).The expression of Tankyrase correlated positively with hTERT(Spearman correlation was 0.553, P < 0.05).But no significant differences were seen between different clinical stages and different pathological grades.

  Conclusion   Tankyrase is overexpressed in gastric adenocarcinoma and correlated positively with hTERT.It may be one of the impor tant factors in gastric adenocarcinomas.