Preventive effect of cristata L flavonoid on osteoporosis in ovariectomized rats

  Objective   To study the effect of cristata L flavonoid on urine mineral and the expression of bone morphogenetic protein 2(BMP2), and phagocytosis function of mononuclear macrophage in ovariectomized rats.

  Methods   Twenty one female SD rats were randomly divided into three groups: Sham, ovariectomized(OVX), ovariectomized with cristata L flavo noidCRIST, 100 mg/(kg°d).The content of urinary calcium, sodium, potassium were measured after ten weeks.The Streptavidin-Peroxidase Histostain^TM-Plus Kits was used to detect the expression of BMP2 in bone.And phagocytosis test of mononuclear macrophage in vitro, carbon clearance measurement were carried out to determine the phagocytotic functions of mononuclear macrophage.

  Results   Compared with Sham group the calcium, sodium in urine expression of ovar iectomized group were higher and the potassium of urine and the expression of BMP2, and the phago-percent age, and phagocytic index and the carbon clearance index were significantly decreased in OVX group(P < 0.05 or < 0.01).Compared with OVX group the urine calcium, sodium expression, and the phago-percentage, carbon phagocytic index were significantly in CRIST group, but the bone BMP2 expression was significantly higher than that of OVX group(P < 0.05 or < 0.01).

  Conclusion   The cristal L flavonoid could decrease the urinary calcium loss, and increased the expression of BMP2, and increased the phagocytotic functions of mononuclear macrophage.It might be advantageous to prevent the postmenopausal Osteoporosis, and to facilitate health care in menopause women.