Study on IgM capturing ELISA kit for detecting hemorrhagic fever with renal syndrome

  Objective   To develop a sensitive, stable and simple diagnostic reagent for rapidly detecting epidemic hemorrhagic fever with renal syndrome(HFRS).

  Methods   The sheep anti-human μ chain was coated in polystyrene plate.The specific McAb of anti-nucleocapsid protein(NP)of Hantaan virus was purified and labeled with horseradish peroxidase (HRP).Recombined nucleocapside protein(rNP)antigen(Ag)was purified by chromato graphy.Both labeled McAb and rNP combined with enzyme substrate system(TMB/H₂O₂)play as a tracer.IgM capture ELISA kit of detecting anti-HFRS virus IgM was established.

  Results   Compaed with indirect ELISA, the total coincident rate of the results of simultaneous determination of 92 serum samples with epidemic hemorrhagic fever was 100%.The specificity, stability and repeatability of IgM capture ELISA kit were excellent.

  Conclusion   IgM capturing ELISA kit is sensitive, reliable and simple.It is applicable to hospitals and institutes detecting epidemic hemorrhagic fever with renal syndrome.It can be used for early diagnosis of epidemic hemorrhagic fever and epidemioligical studies.