Effect of Reduping on lipid over oxidation and apoptosis in peritoneal macrophages of mice under hyperthermia

  Objective   To investigate the protection effect of Reduping against lipid over oxidation and apoptosis in peritoneal macrophages of mice in heat stroke and to provide experimental evidence for the treatment of heat stroke.

  Methods   To establish the heat stroke model under circumstances of dry bulb temperature(34.5±0.5)℃ and(60±5)% relative humidity and 50 mice were randomly divided into five groups(nomal, Reduping, escipient, control and heat strock model groups), superoxide dismutase(SOD)and malondialdehyde(MDA)gel electrophoresis and the apoptosis in peritoneal macrophages were measured by DNA gel electrophoresis and flow cytometer.

  Results   SOD and MDA of Reduping group were higher and lower redpectively than those of any other groups whose difference was significant but similar to normal group; DNA gel electrophoresis of Reduping group had not the ladder of apoptosis; the percentage of apoptosis of Reduping group was lower than that of any other groups but similar to normal group.

  Conclusion   Reduping which can against effectively lipid over oxidation and decrease apoptosis of peritoneal macrophages caused by hyperthermia is an effective medicine against heat stroke.