Variance of antibiotic resistance and virulence gene of Vibrio cholerae O139 in Hangzhou city

  Objective   To investigate the variance of antibiotic resistance and the virulence gene ctxA and tcpA of Virbrio cholerae(VC)O139 strains in Hangzhou from 1994 to 2003.

  Methods   90 VCO139 strains were used to detect the antibiotic resistance and the virulence gene by disc diffusion test(Kirby-Bauer mehtod)and PCR.

  Results   There was no amikacin-resistant strain and 13 strains were both norfloxacin-resistant and ciproflox acin-resistnat.The 100% antibiotic resistance happened to amplicillin in 5 years and SMZ-TMP in 6 years.7 antibiotics were included in the resistance spectrum in 2002 and 2003.And the rate of the strains which carried both ctxA and tcpA was 87.8%.

  Conclusion   The situation of increasing antibiotic resistance is serious.There is obvious difference of antibiotic resistance between VCO139 with ctxA, tcpA and VCO139 without ctxA, tcpA.