Effect of tea polyphenols on cell cycle of human liver cancer cell

  Objective   To study the effects of tea polyphenols on activator protein 1 and cell cycle of human liver cancer cell.

  Methods   An activator protein 1 luciferase reporter gene was constructed and the activities of activator protein 1 were measured.The expression changes of cyclin D₁ and cyclin-denpendent kinase 4 were studied by the immunity organization chemistry method.cell cycle was analyzed by flow cytometry.

  Results   The activities of activator protein 1 were repressed, the experession of cyclin D₁ and cyclin-denpendent kinase 4 was reduced, G₁ arrest was induced by tea polyphenols in cell line.

  Conclusion   Tea polyphenols possibly repressed the expression of cyclin D₁ through down-regulating the activities of activator protein 1;It had nothing to activities of activator protein 1 that tea polyphenols repressed the expression of cyclin-denpendent kinase 4.