Objective To study the effects of tea polyphenols on activator protein 1 and cell cycle of human liver cancer cell.
Methods An activator protein 1 luciferase reporter gene was constructed and the activities of activator protein 1 were measured.The expression changes of cyclin D1 and cyclin-denpendent kinase 4 were studied by the immunity organization chemistry method.cell cycle was analyzed by flow cytometry.
Results The activities of activator protein 1 were repressed, the experession of cyclin D1 and cyclin-denpendent kinase 4 was reduced, G1 arrest was induced by tea polyphenols in cell line.
Conclusion Tea polyphenols possibly repressed the expression of cyclin D1 through down-regulating the activities of activator protein 1;It had nothing to activities of activator protein 1 that tea polyphenols repressed the expression of cyclin-denpendent kinase 4.